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due to excessive diversion of carbon and energy to support PHB synthesis. ...... detector (Agilent RID, G1362A) set on positive polarity and optical unit temperature of ... Agilent RNA Nit following the manufacturer's instructions. ...... Somleva, M.N., Snell, K.D., Beaulieu, J.J., Peoples, O.P., Garrison, B.R. and Patterson, N.A..
                        

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Page 20 of 39

3DJHRI 

Plant Biotechnology Journal Proof

Page 21 of 39

ZĞĨĞƌĞŶĐĞƐ  ůƚƉĞƚĞƌ͕&͕͘:ƵŶŐ͕:͘,͕͘͘;ϮϬϬϬͿdƌĂŶƐŐĞŶŝĐƌĂďŝĚŽƉƐŝƐƉůĂŶƚƐĐĂŶ ĂĐĐƵŵƵůĂƚĞƉŽůLJŚLJĚƌŽdžLJďƵƚLJƌĂƚĞƚŽƵƉƚŽϰйŽĨƚŚĞŝƌĨƌĞƐŚǁĞŝŐŚƚ͘WůĂŶƚĂϮϭϭ͕ϴϰϭͲ ϴϰϱ͘ ƌŽƵƋƵŝƐƐĞ͕Z͕͘'ĂƵĚŝůůĞƌĞ͕:͘W͘ĂŶĚZĂLJŵŽŶĚ͕W͘;ϭϵϵϴͿ/ŶĚƵĐƚŝŽŶŽĨĂĐĂƌďŽŶͲƐƚĂƌǀĂƚŝŽŶͲ ƌĞůĂƚĞĚƉƌŽƚĞŽůLJƐŝƐŝŶǁŚŽůĞŵĂŝnjĞƉůĂŶƚƐƐƵďŵŝƚƚĞĚƚŽ>ŝŐŚƚͬĂƌŬĐLJĐůĞƐĂŶĚƚŽ ĞdžƚĞŶĚĞĚĚĂƌŬŶĞƐƐ͘WůĂŶƚWŚLJƐŝŽůϭϭϳ͕ϭϮϴϭͲϭϮϵϭ͘ ƌŽƵƋƵŝƐƐĞ͕Z͕͘:ĂŵĞƐ͕&͕͘WƌĂĚĞƚ͕͘ĂŶĚZĂLJŵŽŶĚ͕W͘;ϭϵϵϮͿƐƉĂƌĂŐŝŶĞŵĞƚĂďŽůŝƐŵĂŶĚ ŶŝƚƌŽŐĞŶĚŝƐƚƌŝďƵƚŝŽŶĚƵƌŝŶŐƉƌŽƚĞŝŶĚĞŐƌĂĚĂƚŝŽŶŝŶƐƵŐĂƌͲƐƚĂƌǀĞĚŵĂŝnjĞƌŽŽƚƚŝƉƐ͘

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WůĂŶƚĂϭϴϴ͕ϯϴϰͲϯϵϱ͘

ƌŽƵƋƵŝƐƐĞ͕Z͕͘:ĂŵĞƐ͕&͕͘ZĂLJŵŽŶĚ͕W͘ĂŶĚWƌĂĚĞƚ͕͘;ϭϵϵϭͿ^ƚƵĚLJŽĨŐůƵĐŽƐĞƐƚĂƌǀĂƚŝŽŶŝŶ ĞdžĐŝƐĞĚŵĂŝnjĞƌŽŽƚƚŝƉƐ͘WůĂŶƚWŚLJƐŝŽůϵϲ͕ϲϭϵͲϲϮϲ͘

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ŚĂĐŬŽ͕͕͘ĂƌŬĞƌ͕͘:͕͘ĞĂŐůĞLJ͕͘Ͳ͕͘dŝŵŵŝŶƐ͕E͕͘͘ŚƌLJƐĂŶƚŚŽƉŽƵůŽƐ͕W͕͘:ĂĐŽď͕^͘^͕͘ 'ƌĂLJ͕W͘ĂŶĚEŝĞůƐĞŶ͕>͘ĞĂ͕W͘ĂŶĚDŝĨůŝŶ͕͘;ϭϵϴϬͿdƌĂŶƐƉŽƌƚĂŶĚŵĞƚĂďŽůŝƐŵŽĨĂƐƉĂƌĂŐŝŶĞĂŶĚŽƚŚĞƌŶŝƚƌŽŐĞŶ ĐŽŵƉŽƵŶĚƐǁŝƚŚŝŶƚŚĞƉůĂŶƚ͘/Ŷ͗dŚĞďŝŽĐŚĞŵŝƐƚƌLJŽĨƉůĂŶƚƐ;WĞĂ͕W͘:͕͘ZŽďŝŶƐŽŶ͕^͘͘ĂŶĚ^ƚĞǁĂƌƚ͕'͘Z͘;ϭϵϵϬͿdŚĞĞŶnjLJŵŽůŽŐLJĂŶĚŵĞƚĂďŽůŝƐŵŽĨ ŐůƵƚĂŵŝŶĞ͕ŐůƵƚĂŵĂƚĞ͕ĂŶĚĂƐƉĂƌĂŐŝŶĞ͘/Ŷ͗dŚĞďŝŽĐŚĞŵŝƐƚƌLJŽĨƉůĂŶƚƐ;W͕͘͘WĞƚƌĂƐŽǀŝƚƐ͕>͕͘͘^ŶĞůů͕͕͘͘DŝƌŬŽǀ͕d͕͘͘ĚĂ^ŝůǀĂ͕ :͕͘:ŝĨŽŶ͕:͕͘ZĂŝ͕D͕͘^ĐŚŶĞůů͕Z͘:͕͘ƌƵŵďůĞLJ͕^͘D͕͘>ĂŬƐŚŵĂŶĂŶ͕W͕͘ŽŵƐƚŽĐŬ͕:͘͘ĂŶĚ WĂƚĞƌƐŽŶ͕͘,͘;ϮϬϬϲͿ^ƵŐĂƌĐĂŶĞ/ŵƉƌŽǀĞŵĞŶƚƚŚƌŽƵŐŚƌĞĞĚŝŶŐĂŶĚŝŽƚĞĐŚŶŽůŽŐLJ͘ WůƌĞĚZĞϮϳ͕ϭϱͲϭϭϴ͘ DŽƌĂŶĚŝŶŝ͕W͘;ϮϬϭϯͿŽŶƚƌŽůůŝŵŝƚƐĨŽƌĂĐĐƵŵƵůĂƚŝŽŶŽĨƉůĂŶƚŵĞƚĂďŽůŝƚĞƐ͗ďƌƵƚĞĨŽƌĐĞŝƐŶŽ ƐƵďƐƚŝƚƵƚĞĨŽƌƵŶĚĞƌƐƚĂŶĚŝŶŐ͘WůĂŶƚŝŽƚĞĐŚŶŽů:ϭϭ͕ϮϱϯͲϮϲϳ͘

3DJHRI 

Plant Biotechnology Journal Proof

Plant Biotechnology Journal Proof

EĂǁƌĂƚŚ͕͕͘WŽŝƌŝĞƌ͕z͘ĂŶĚ^ŽŵĞƌǀŝůůĞ͕͘;ϭϵϵϰͿdĂƌŐĞƚŝŶŐŽĨƚŚĞƉŽůLJŚLJĚƌŽdžLJďƵƚLJƌĂƚĞ ďŝŽƐLJŶƚŚĞƚŝĐƉĂƚŚǁĂLJƚŽƚŚĞƉůĂƐƚŝĚƐŽĨƌĂďŝĚŽƉƐŝƐƚŚĂůŝĂŶĂƌĞƐƵůƚƐŝŶŚŝŐŚůĞǀĞůƐŽĨ ƉŽůLJŵĞƌĂĐĐƵŵƵůĂƚŝŽŶ͘WƌŽĐEĂƚůĐĂĚ^Đŝh^ϵϭ͕ϭϮϳϲϬͲϭϮϳϲϰ͘ EĞů͕^͘;ϮϬϭϬͿdŚĞƉŽƚĞŶƚŝĂůŽĨďŝŽƚĞĐŚŶŽůŽŐLJŝŶƚŚĞƐƵŐĂƌĐĂŶĞŝŶĚƵƐƚƌLJ͗ƌĞLJŽƵƌĞĂĚLJĨŽƌ ƚŚĞŶĞdžƚĞǀŽůƵƚŝŽŶ͍/Ŷƚ^ƵŐĂƌ:ϭϭϮ͕ϭϭͲϭϲ͘ EŝĞůƐĞŶ͕d͘,͕͘ZƵŶŐ͕:͘,͘ĂŶĚsŝůůĂĚƐĞŶ͕͘;ϮϬϬϰͿ&ƌƵĐƚŽƐĞͲϮ͕ϲͲďŝƐƉŚŽƐƉŚĂƚĞ͗ĂƚƌĂĨĨŝĐƐŝŐŶĂů ŝŶƉůĂŶƚŵĞƚĂďŽůŝƐŵ͘dƌĞŶĚƐWůĂŶƚ^Đŝϵ͕ϱϱϲͲϱϲϯ͘ KŬĂŵŽƚŽ͕D͕͘sŝĚŵĂƌ͕:͘:͘ĂŶĚ'ůĂƐƐ͕͘͘D͘;ϮϬϬϯͿZĞŐƵůĂƚŝŽŶŽĨEZdϭĂŶĚEZdϮ'ĞŶĞ &ĂŵŝůŝĞƐŽĨƌĂďŝĚŽƉƐŝƐƚŚĂůŝĂŶĂ͗ZĞƐƉŽŶƐĞƐƚŽEŝƚƌĂƚĞWƌŽǀŝƐŝŽŶ͘WůĂŶƚĞůůWŚLJƐŝŽů

Fo

ϰϰ͕ϯϬϰͲϯϭϳ͘

KƌƐĞů͕D͕͘ƵůĞŶďƵƌŐ͕͕͘͘DĐYƵĂůƚĞƌ͕Z͕͘͘'ĞďďŝĞ͕>͕͘͘͘͘WƵƌŶĞůů͕D͘W͕͘EŝĞůƐĞŶ͕>͕͘͘͘ŚĂŽ͕>͕͘DĐYƵĂůƚĞƌ͕Z͕͘͘^ŶĞůů͕͘͘;ϮϬϭϭͿƐŽůƵďůĞĂĐŝĚ ŝŶǀĞƌƚĂƐĞŝƐĚŝƌĞĐƚĞĚƚŽƚŚĞǀĂĐƵŽůĞďLJĂƐŝŐŶĂůĂŶĐŚŽƌŵĞĐŚĂŶŝƐŵ͘:WůĂŶƚWŚLJƐŝŽů ϭϲϴ͕ϵϴϯͲϵϴϵ͘

ev

ZĞĚĚLJ͕͘Z͘ĂŶĚĂƐ͕s͘^͘Z͘;ϭϵϴϳͿDŽĚƵůĂƚŝŽŶŽĨ^ƵĐƌŽƐĞŽŶƚĞŶƚďLJ&ƌƵĐƚŽƐĞϮ͕ϲͲ

ie

ŝƐƉŚŽƐƉŚĂƚĞĚƵƌŝŶŐWŚŽƚŽƐLJŶƚŚĞƐŝƐŝŶZŝĐĞ>ĞĂǀĞƐ'ƌŽǁŝŶŐĂƚŝĨĨĞƌĞŶƚ>ŝŐŚƚ /ŶƚĞŶƐŝƚŝĞƐ͘:džƉŝŽůϯϴ͕ϴϮϴͲϴϯϯ͘

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ZŽƐƐĂƚŽ͕>͕͘>ĂŝŶĠ͕W͘ĂŶĚKƵƌƌLJ͕͘;ϮϬϬϭͿEŝƚƌŽŐĞŶƐƚŽƌĂŐĞĂŶĚƌĞŵŽďŝůŝnjĂƚŝŽŶŝŶƌĂƐƐŝĐĂ ŶĂƉƵƐ>͘ĚƵƌŝŶŐƚŚĞŐƌŽǁƚŚĐLJĐůĞ͗ŶŝƚƌŽŐĞŶĨůƵdžĞƐǁŝƚŚŝŶƚŚĞƉůĂŶƚĂŶĚĐŚĂŶŐĞƐŝŶ

On

ƐŽůƵďůĞƉƌŽƚĞŝŶƉĂƚƚĞƌŶƐ͘:džƉŽƚϱϮ͕ϭϲϱϱͲϭϲϲϯ͘

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1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Plant Biotechnology Journal Proof

WŚLJƐŝŽůŽŐLJ͕ŝŽĐŚĞŵŝƐƚƌLJ͕ĂŶĚ&ƵŶĐƚŝŽŶĂůŝŽůŽŐLJ;DŽŽƌĞ͕W͘,͘ĂŶĚŽƚŚĂ͕&͘͘ĞĚƐͿ͕ ƉƉ͘ϭϮϭͲϭϱϰ͘ŚŝĐŚĞƐƚĞƌ͕hƚĚ͘

^ĂŬĂŬŝďĂƌĂ͕,͕͘ŝŐŚƚŽŶƚŚĞdžƉƌĞƐƐŝŽŶŽĨ'ůƵƚĂŵŝŶĞ^LJŶƚŚĞƚĂƐĞƐĂŶĚ&ĞƌƌĞĚŽdžŝŶͲĞƉĞŶĚĞŶƚ 'ůƵƚĂŵĂƚĞ^LJŶƚŚĂƐĞŝŶDĂŝnjĞ͘WůĂŶƚĞůůWŚLJƐŝŽůϯϯ͕ϭϭϵϯͲϭϭϵϴ͘ ^ĂƌƵƵů͕W͕͘^ƌŝĞŶĐ͕&͕͘^ŽŵĞƌƐ͕͘͘ĂŶĚ^ĂŵĂĐ͕͘͘;ϮϬϬϮͿWƌŽĚƵĐƚŝŽŶŽĨĂďŝŽĚĞŐƌĂĚĂďůĞ ƉůĂƐƚŝĐƉŽůLJŵĞƌ͕ƉŽůLJͲďͲŚLJĚƌŽdžLJďƵƚLJƌĂƚĞ͕ŝŶƚƌĂŶƐŐĞŶŝĐĂůĨĂůĨĂ͘ƌŽƉ^ĐŝϰϮ͕ϵϭϵͲϵϮϳ͘ ^ĐŚůƵƚĞƌ͕h͕͘ŽůŵƐĞĞ͕͕͘^ĐŚŽůnj͕h͕͘ƌĂƵƚŝŐĂŵ͕͕͘tĞďĞƌ͕͘W͕͘ĞůůĞƌŚŽĨĨ͕E͕͘ƵĐŚĞƌ͕D͕͘ &ĂŚŶĞŶƐƚŝĐŚ͕,͘ĂŶĚ^ŽŶŶĞǁĂůĚ͕h͘;ϮϬϭϯͿĚĂƉƚĂƚŝŽŶŽĨŵĂŝnjĞƐŽƵƌĐĞůĞĂĨ

3DJHRI 

Plant Biotechnology Journal Proof

Plant Biotechnology Journal Proof

ŵĞƚĂďŽůŝƐŵƚŽƐƚƌĞƐƐƌĞůĂƚĞĚĚŝƐƚƵƌďĂŶĐĞƐŝŶĐĂƌďŽŶ͕ŶŝƚƌŽŐĞŶĂŶĚƉŚŽƐƉŚŽƌƵƐ ďĂůĂŶĐĞ͘D'ĞŶŽŵŝĐƐϭϰ͕ϰϰϮ͘ ^ĐŚůƵƚĞƌ͕h͕͘DĂƐĐŚĞƌ͕D͕͘ŽůŵƐĞĞ͕͕͘^ĐŚŽůnj͕h͕͘ƌĂƵƚŝŐĂŵ͕͕͘&ĂŚŶĞŶƐƚŝĐŚ͕,͘ĂŶĚ ^ŽŶŶĞǁĂůĚ͕h͘;ϮϬϭϮͿDĂŝnjĞƐŽƵƌĐĞůĞĂĨĂĚĂƉƚĂƚŝŽŶƚŽŶŝƚƌŽŐĞŶĚĞĨŝĐŝĞŶĐLJĂĨĨĞĐƚƐŶŽƚ ŽŶůLJŶŝƚƌŽŐĞŶĂŶĚĐĂƌďŽŶŵĞƚĂďŽůŝƐŵďƵƚĂůƐŽĐŽŶƚƌŽůŽĨƉŚŽƐƉŚĂƚĞŚŽŵĞŽƐƚĂƐŝƐ͘ WůĂŶƚWŚLJƐŝŽůϭϲϬ͕ϭϯϴϰͲϭϰϬϲ͘ ^ŚĞŶƚŽŶ͕D͕͘&ŽŶƚĂŝŶĞ͕s͕͘,ĂƌƚǁĞůů͕:͕͘DĂƌƐŚ͕:͘d͕͘:ĞŶŬŝŶƐ͕'͘/͘ĂŶĚEŝŵŵŽ͕,͘'͘;ϮϬϬϲͿ ŝƐƚŝŶĐƚƉĂƚƚĞƌŶƐŽĨĐŽŶƚƌŽůĂŶĚĞdžƉƌĞƐƐŝŽŶĂŵŽŶŐƐƚŵĞŵďĞƌƐŽĨƚŚĞWWĐĂƌďŽdžLJůĂƐĞ ŬŝŶĂƐĞŐĞŶĞĨĂŵŝůLJŝŶϰƉůĂŶƚƐ͘WůĂŶƚ:ϰϴ͕ϰϱͲϱϯ͘

Fo

^ŝĞĐŝĞĐŚŽǁŝĐnj͕>ĂďŽƌĂƚŽƌLJŶĂůLJƚŝĐĂůWƌŽĐĞĚƵƌĞ͘фŚƚƚƉ͗ͬͬĚĞǀĂĨĚĐ͘ŶƌĞů͘ŐŽǀͬƉĚĨƐͬϵϯϲϬ͘ƉĚĨ͘ ^ŵŽůŬĞ͕͘͘ĂŶĚ^ŝůǀĞƌ͕W͘͘;ϮϬϭϭͿ/ŶĨŽƌŵŝŶŐŝŽůŽŐŝĐĂůĞƐŝŐŶďLJ/ŶƚĞŐƌĂƚŝŽŶŽĨ^LJƐƚĞŵƐ

ev

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