Oct 26, 2015 - Heiner Boeing, Nuthetal ... Hannelore Daniel, Freising-Weihenstephan on behalf .... Bacteroides group increased in obese rats that received B.
Ann Nutr Metab 2015;67(suppl 1):1–601 DOI: 10.1159/000440895
Published online: October 20, 2015
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12th European Nutrition Conference (FENS) Berlin, Germany, October 20–23, 2015
Abstracts Guest Editors
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Heiner Boeing, Nuthetal Helmut Oberritter, Bonn Hannelore Daniel, Freising-Weihenstephan on behalf of the German Nutrition Society
149/888. Effects of three probiotic strains on the intestinal microbiota composition of Zucker rats
Conclusions: The highest concentrations of leptin were associa-
Author(s): (1) Julio Plaza Díaz; (2) Candido Robles Sanchez; (3) Luis Fontana; (4) Natalia Chueca; (4) Jose Angel Fernandez-Caballero; (5) Federico Garcia; (6) Maria Jose Saez-Lara; (7) Fernando Romero; (8) Angel Gil; (8) Carolina Gomez-Llorente.
Keywords: (maximum 5): leptin, smoking, behavior addictive,
Affiliation: (1) Ph D in Biochemistry and Molecular Biology/ Dept.
ted with greater craving and difficulty in achieve abstinence.
substance withdrawal syndrome.
149/878. Betaine homocysteine methyltransferase exerts metabolic effects beyond controlling methionine homeostasis. Author(s): (1) Thomas U.R. Gantert; (2) Adriano Maida; (2) Stephan Herzig; (2) Adam J. Rose. Affiliation: (1) Nutritional Scientist, German Center Research Center Heidelberg, Germany; (2) Scientist Metabolism, German Cancer Research Center Heidelberg, Germany.
Introduction: Betaine homocysteine methyltransferase (Bhmt) catalyses methionine production from homocysteine in liver. Methionine can be converted to S-adenosyl methionine (SAM), a methyldonor for a multitude of methylation reactions. Bhmt deficient mice have been shown to have altered concentrations of liver and serum lipids. Upon dietary protein restriction (DPR) Bhmt expression is paradoxically downregulated in spite of a proposed methionine sparing effect of Bhmt. Objectives: The aim of the study was to elucidate the effects of a reintroduction of Bhmt in a natural setting of low Bhmt expression, i.e. during DPR, and evaluate its metabolic effects. Method / Design: Adenoviral mediated overexpression of Bhmt in male C57Bl6/N mice fed a standard protein diet (20% protein) or a protein restricted diet (5% protein): Eight week old mice received either Ad-Bhmt or Ad-NC. Experimental diets were switched the day after and given ad libitum for eight consecutive days until sacrifice. Six mice were assigned to each of the four conditions (n=24). Results: Overexpression of Bhmt in the liver of mice results in liver enlargements by ~15-20%. Liver SAM, SAH and SAM:SAH ratio as well as plasma and liver methionine and homocysteine were not affected. Similarly, lipid metabolites such as triglycerides and choline containing phospholipids were largely unaffected by overexpression of Bhmt. However, plasma levels of glycine and serine were reduced in protein restricted mice overexpressing Bhmt. Conclusions: Bhmt overexpression resulted in only subtle changes in metabolic outcomes normally associated with the function of Bhmt. During DPR Bhmt may help to regulate plasma glycine and serine levels, a novel function of Bhmt beyond controlling methionine homeostasis. Keywords: (maximum 5): Bhmt, amino acid metabolism, die-
tary protein
12th European Nutrition Conference 2015
Biochemistry & Molecular Biology II, School of Pharmacy, and Institute of Nutrition and Food Technology “José Mataix”, Biomedical Research Center, University of Granada, Spain; (2) Biochemist/Dept. Biochemistry & Molecular Biology II, School of Pharmacy, and Institute of Nutrition and Food Technology “José Mataix”, Biomedical Research Center, University of Granada, Spain; (3) Ph D./Dept. Biochemistry & Molecular Biology II, School of Pharmacy, and Institute of Nutrition and Food Technology “José Mataix”, Biomedical Research Center, University of Granada, Spain; (4) Biologist/Dept. Microbiology, Complejo Hospitalario Universitario de Granada, Instituto de Investigacion Biosanitaria (IBS), Granada, Spain.; (5) Ph D/Dept. Microbiology, Complejo Hospitalario Universitario de Granada, Instituto de Investigacion Biosanitaria (IBS), Granada, Spain.; (6) Ph D/Dept. Biochemistry & Molecular Biology I, School of Sciences, University of Granada, Spain; (7) Ph D/R&D Dept., Hero Spain S. A., Murcia, Spain; (8) Ph D/Dept. Biochemistry & Molecular Biology II, School of Pharmacy, and Institute of Nutrition and Food Technology “José Mataix”, Biomedical Research Center, University of Granada, Spain.
Introduction: Current attempts to manipulate the gastrointestinal microbiota focus on finding remedies for several health disorders. Probiotics are consumed as treatments for various gastrointestinal tract dysfunctions. However, their actual ability to affect gut microbiota is still under debate Objectives: We investigated the effects of L. paracasei CNCM I-4034, L. rhamnosus CNCM I-4036 and B. breve CNCM I-4035 feeding on the fecal microbiota composition in a genetic animal model of obesity Method / Design: Forty-eight Zucker-Leprfa/fa and 16 Zuckerlean+/fa male rats weighing 168-180 g were used. Eight Zucker-lean+/ fa and 8 Zucker-Leprfa/fa rats were euthanized (baseline). The remaining 40 Zucker-Leprfa/fa rats were randomly assigned to receive 1010 CFUs of one of the three strains, a mixture or a placebo by oral administration each day for 30 days. An additional group of 8 Zuckerlean+/fa rats received placebo for 30 days. Fecal samples were taken to perform fluorescence in situ hybridization (FISH) and 16S rRNA gene pyrosequencing. Lipopolysaccharide binding protein (LBP) was measured in serum Results: FISH analysis of the feces revealed changes in bacterial groups. Bacteroides group increased in obese rats that received B. breve, L. rhamnosus or the mixture of two probiotic strains. Closdridium perfringens and Closdridium difficile increased in the feces of both obese rats fed L. paracasei and lean rats fed the placebo. Compared with obese rats fed the placebo, 16S rRNA sequencing found the following reductions: Parabacteroides in rats fed L. rhamnosus, Leptospiraceae in rats fed L. paracasei, Halanaerobiaceae in rats fed B. Ann Nutr Metab 2015; 67(suppl 1) 279
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higher scores of QSU-Brief (Factor 2), after 1 month of treatment (n=15 - 53.6%; p=0.030). There was a positive correlation between leptin/BMI and leptin/%BF with the QSU-Brief (Factor 2) of 1 month in women who remained smokers (r=0.565; p=0.023) and the QSU-Brief (Factor 2) initial among the abstinent women (r=0.551; p=0.033).
Conclusions: Results obtained suggest that feeding Zucker-Leprfa/fa rats with these probiotic strains modified the fecal microbiota composition and reduced the serum levels of LBP. Funded by Hero Spain, S. A. through grant FEUGR-3945. Keywords: (maximum 5): Intestinal microbiota, probiotics
149/898. Energy expenditure in patients with short bowel syndrome: a doubly labeled water study Author(s): (1) Priscila Fassini; (2) Eduardo Ferriolli; (2) Júlio Marchini; (2) Karina Pfrimer. Affiliation: (1) MS. Nutritionist. University of São Paulo. Ribeirão
Preto. Brazil; (2) PhD. University of São Paulo. Ribeirão Preto. Brazil.
Introduction: Short Bowel Syndrome (SBS) is a set of signs and symptoms resulting from nutritional and metabolic alterations due to extensive resection of the small intestine, and represent one of the most severe states of malabsorption. Objectives: To compare the total energy expenditure (TEE) measured by the doubly labeled water (DLW) method with its estimate by an activity monitor in SBS volunteers and control group. Method / Design: Eleven volunteers (6 women, 5 men) were evaluated with SBS and a control group (CG) with volunteers without SBS, with similar characteristics (gender, age, ethnicity, BMI) of SBS group. Dose of DLW (2g of 10%18O-labelled and 0.12g of 99.9%deuterium-labelled water/Kg estimated total body water) was ingested by the volunteers after collecting a dose of basal urine. TEE measured (DLW) was compared with its estimate by activity monitor activPALTM (14 days). MET obtained by the monitor was multiplied by the resting energy expenditure (REE), measured by indirect calorimetry and was considered the thermic effect of food (10%). Pearson´s correlation and Intraclass Correlation Coefficient to compare energy expenditure (p˂0.05). Results: Age was 53±8 years for both groups. REE showed no significant differences. TEE (DLW) showed a significant difference between groups (p< 0.01): 1835±276kcal for SBS and 2396±448kcal for CG. Estimated TEE was 2058±301kcal for the SBS and 2199±340kcal for the CG without significant difference between groups. There was a moderate positive correlation between TEE (DLW) and estimated in both groups (r=0.726, p=0.01 in the GC; r=0.771, p=0.005 in SBS). Conclusions: TEE estimated overestimates energy expenditure by 9.8% of the measured value in SBS group, and underestimates at 8.2% in CG. Use of activPALTM monitor could be considered a good method for the determination of TEE in these individuals, once presented a good correlation with the values recorded by the DLW method. Keywords: (maximum 5): short bowel syndrome, energy ex-
penditure, doubly labeled water
280 Ann Nutr Metab 2015; 67(suppl 1)
149/909. Effect of maternal low quality protein diet on fetal growth and development Author(s): (1) Asli Akyol; (2) Arzu Kabasakal; (3) Halil Dasgin; (2) Atila Gulec; (4) Ilyas Onbasilar. Affiliation: (1) Nutritionist.Department of Nutrition and Dietetics.
Hacettepe University.Turkey; (2) Nutritionist.Department of Nutrition and Dietetics.Hacettepe University.Ankara.Turkey; (3) Nutritionist.Department of Nutrition and Dietetics.Kirikkale University. Kirikkale.Turkey; (4) Lecturer.Faculty of Medicine.Hacettepe University.Ankara.Turkey.
Introduction: A large body of evidence have shown the adverse effects of sub-optimal intra-uterine environment on fetal growth and development in epidemiological and animal studies. In this context many studies have indicated the influence of maternal low protein diets on fetal programming. However the effect of a maternal low quality protein diet on fetal growth and development is unknown. Objectives: The main objective of this study was to examine the effect of maternal low quality protein diet on fetal growth and development Method / Design: Wistar rats (11 weeks old) were mated and maintained on either chow diet with 20 % casein (n=6) as the control group (C), or low quality protein diet with 20 % wheat gluten as the experimental group (W). At the end of lactation offspring were kept on chow diet with 20 % casein until 20 weeks old. Food intake and body weight were recorded daily. Plasma and tissue samples were stored at -80°C after culling. Results: Maternal body weights were similar in both groups throughout the study (C:245.06±6.46 g, W: 249.28±5.46, P>0.05). Despite similar body weights, W group consumed significantly lower amount of food when compared to C (C:25.09±0.51 g, W: 23.59±0.43, P=0.03). Although birth weight of offspring was significantly higher in W group (C:5.65±0.06 g, W: 5.81±0.05, P
