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Nov 3, 1989 - Kurt Weising, Franz Weigand3, Albert J.Driesell, Gunter Kahl, Hans Zischler2 and Jorg T.Epplen2. Plant Molecular Biology Group, University of ...

Nucleic Acids Research

Volume 17 Number 23 1989

Polymorphic simple GATA/GACA repeats in plant genomes Kurt Weising, Franz Weigand3, Albert J.Driesell, Gunter Kahl, Hans Zischler2 and Jorg T.Epplen2 Plant Molecular Biology Group, University of Frankfurt, 1DECHEMA, Frankfurt, 2Max-Planck-Institut fiir Psychiatrie, Martinsried, FRG and 3ICARDA, Aleppo, Syria Submitted November 3, 1989

simple quadruplet repeats (GATA)n and (GACA)n are constituents of repetitive DNA in most animal genomes investigated to date (1). Since restriction fragments harbouring these sequences show considerable polymorphism between individuals, GATA/GACA repeats have been successfully used for DNA fingerprinting of e.g. human DNA (2). In this report, we demonstrate the presence of tandemly arranged (GATA)n and (GACA)n repeats in the genome of chickpea (Cicer arietinum) and barley (Hordeum vulgare), a dicot and a monocot crop plant, respectively (Fig. 1). DNA was isolated according to (3) from leaves of individual plants derived from different accessions (collection of ICARDA, Syria), digested with Alu I or Taq I and electrophoretically separated on 1.2 % agarose gels. Gels were denatured, neutralized, dried and hybridized to 32P-labeled synthetic (GATA)4 or (GACA)4 oligonucleotide probes (2). As shown in Fig.1, both GATA- and GACA repeats are present in the genomes of chickpea and barley. Whereas band heterogeneity is limited in case of barley/GATA and chickpea/GACA, hybridization of chickpea DNA to the (GATA)4 probe and of barley DNA to the (GACA)4 probe resulted in considerable band diversity. These results confirm and extend recent data obtained by hybridizing human and M13 minisatellite probes to several plant DNAs (4,5). Furthermore, they demonstrate the ubiquitous presence and polymorphism of simple repetitive DNA motives in the eukaryotic kingdoms. The applicability of these probes for variety identification in crop plants is presently under investigation. The

Fig.l: Hybridization of Alu I- and Taq I-digested barley and chickpea DNA to (GACA)4 and (GATA)4. DNA (5ug per lane) was isolated from individual ...

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Refs.: (1) Epplen,J.T. (1988), J.Hered. 79, 409-417. (2) Ali,S., Muller,C.R., Epplen, J.T. (1986) Hum.Genet. 74,239243. (3) Saghai-Maroof, M.A., Soliman, K.M.,Jorgensen,R.A., Allard, R.W. (1984) Proc. Natl. Acad. Sci.USA 81, 8014-8018. (4) Dallas,J.F. (1988) Proc.Natl.Acad.Sci. USA 85, 6831-6835. (5) Rogstad, S.H., Patton II,J.C., Schaal, B.A. (1988) Proc.Natl.Acad. Sci. USA, 85, 9176-9178.

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