A Microfluidic Device for Temporally Controlled Gene Expression and

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Jan 23, 2008 - electric control valves to switch media (see Fig. S1). We could ...... Costanzo M, Nishikawa JL, Tang X, Millman JS, Schub O, et al. (2004).

A Microfluidic Device for Temporally Controlled Gene Expression and Long-Term Fluorescent Imaging in Unperturbed Dividing Yeast Cells Gilles Charvin1*, Frederick R. Cross2, Eric D. Siggia1 1 Center For Studies in Physics and Biology, The Rockefeller University, New York, New York, United States of America, 2 The Rockefeller University, New York, New York, United States of America

Background. Imaging single cells with fluorescent markers over multiple cell cycles is a powerful tool for unraveling the mechanism and dynamics of the cell cycle. Over the past ten years, microfluidic techniques in cell biology have emerged that allow for good control of growth environment. Yet the control and quantification of transient gene expression in unperturbed dividing cells has received less attention. Methodology/Principal Findings. Here, we describe a microfluidic flow cell to grow Saccharomyces Cerevisiae for more than 8 generations (

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