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ABCD - PLOS
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ABCD - PLOS
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specific lysis. BHRF1-specific CD8+ T cell clones. D. EBNA2-specific CD8+ T cell clones. 0. 0.5. 1.
QPR
. IFNγ (ng/ml). 0. 1. TSS. MVA EBNA2. MVA EBNA3B.
B IFNg (ng/ml)
Peptide pool # HLA-A 1 2 25 68 1 2 1 3 2 24 2 11 2 24 1
-B 39 40 15 39 8 39 7 40 27 35 40 51 44 55 8 57
14.1 14.2 14.3 14.4 14.5
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
IFNg (ng/ml)
Donor 8 0.4 0.3 0.2 0.1 0
BHRF1 Donor 9 0.8 0.6 0.4 0.2 0 1 2 3 4 5 6 Peptide pool #
Peptide #
-C 0304 12 0303 12 7 0304 7 2 4 0304 15 0303 16 6 8
HLA-A 68
-B 44
Peptide #
-C 7
3 68 35 44 4 5 1 2 44 55 3 5 12 2 8 44 3 11
2 11 15 40 3 7 0 1 2 3 IFNg (ng/ml)
0 0.5 1 1.5 2 2.5 IFNg (ng/ml)
Peptide sequence 14.2 KPGGPWRPEPNTSSPSMPEL 14.3 WRPEPNTSSPSMPELSPVLG 14.4 NTSSPSMPELSPVLGLHQGQ TSSPSMPEL No peptide
C
3.1 3.2 3.3 3.4 3.5 3.6
EBNA2
A
EBNA2-specific CD8+ T cell clones
IFNg (ng/ml)
Peptide sequence
IFNg (ng/ml)
0.45
3.1 NSETFTETWNRFITH ETFTETWNR No peptide
0.57 0.6 0.0
0.6 0.09
D
BHRF1-specific CD8+ T cell clones vBHRF1
MVA EBNA2 MVA EBNA3B
TSS 0
0 0.5 1 IFNg (ng/ml)
vTK-
QPR
1
ETF
SRV
0 10 20 30 40 0 10 20 % specific lysis
30
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