in anaerobic chemostat cultures. A. M. Breure, H. H. Beeftink, J. Verkuijlen, and J. G. van Andel. Laboratorium voor Microbiologie, Laboratorium voor Chemische ...
Acidogenic fermentation of protein/carbohydrate mixtures by bacterial populations adapted to one of the substrates in anaerobic chemostat cultures A. M. Breure, H. H. Beeftink, J. Verkuijlen, and J. G. van Andel Laboratorium voor Microbiologie, Laboratorium voor Chemische Technologie, Universiteit van Amsterdam, Nieuwe Achtergracht 127, 1018 WS Amsterdam, The Netherlands
Summary. The influence of the adaptation procedure on the simultaneous fermentation of glucose and gelatin by putatively carbon-limited mixed anaerobic bacterial populations was investigated. In one series of experiments glucose, dissolved in a mineral salts solution, was fed to mixed populations of bacteria in anaerobic carbon-limited chemostat cultures maintained at different pH values and at 30 ° C. When, after reaching a steady state, the carbon substrate was switched to gelatin, growth ceased. However, when gelatin was added to the medium as a second carbon substrate, it was found in all cases that hydrolysis and fermentation of the protein proceeded to a limited extent (
