Adverse pregnancy outcomes and imbalance in angiogenic ... - PLOS

2 downloads 0 Views 1MB Size Report
Jul 17, 2018 - Archer Turpin3☯, Richard K. D. Ephraim4‡, Linda Ahenkorah ..... using Chi-square test for two by three or more contingency and Fisher's exact.
RESEARCH ARTICLE

Adverse pregnancy outcomes and imbalance in angiogenic growth mediators and oxidative stress biomarkers is associated with advanced maternal age births: A prospective cohort study in Ghana Enoch Odame Anto1,2☯*, William K. B. A. Owiredu1☯, Samuel Asamoah Sakyi1☯, Cornelius Archer Turpin3☯, Richard K. D. Ephraim4‡, Linda Ahenkorah Fondjo1‡, Christian Obirikorang1‡, Eric Adua2‡, Emmanuel Acheampong1‡

a1111111111 a1111111111 a1111111111 a1111111111 a1111111111

1 Department of Molecular Medicine, School of Medical Sciences, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana, West Africa, 2 School of Medical and Health Sciences (SMHS), Edith Cowan University (ECU), Perth, Western Australia, Australia, 3 Department of Obstetrics and Gynaecology, Komfo Anokye Teaching Hospital, Kumasi, Ghana, West-Africa, 4 Department of Medical Laboratory Technology, University of Cape Coast, Cape Coast, Ghana, West Africa ☯ These authors contributed equally to this work. ‡ These authors also contributed equally to this work. * [email protected]

OPEN ACCESS Citation: Odame Anto E, Owiredu WKBA, Sakyi SA, Turpin CA, Ephraim RKD, Fondjo LA, et al. (2018) Adverse pregnancy outcomes and imbalance in angiogenic growth mediators and oxidative stress biomarkers is associated with advanced maternal age births: A prospective cohort study in Ghana. PLoS ONE 13(7): e0200581. https://doi.org/ 10.1371/journal.pone.0200581 Editor: Cheryl S. Rosenfeld, University of Missouri Columbia, UNITED STATES

Abstract Background Advanced maternal age (AMA) has been associated with negative pregnancy outcomes. Oxidative stress (OS) and defective placental dysfunction are contributing factors. This study determined the association between AMA and adverse pregnancy outcomes, OS biomarkers and angiogenic growth mediators (AGMs) in normal pregnancies.

Received: April 29, 2017

Methods

Accepted: July 1, 2018

This prospective cohort study conducted at the Obstetrics and Gynaecology (O&G) Department of the Komfo Anokye Teaching Hospital (KATH) finally included 175 normal pregnant women comprising, 58 AMA (35–45 years), 55 (30–34 years) and 62 optimal childbearing age (20–29 years). Venous blood samples were collected at 28–32 weeks for soluble fmslike tyrosine kinase-1 (sFlt-1), placental growth factor (PIGF), 8-epiprostaglandinF2-α (8epi-PGF2α) and total antioxidant capacity (TAC) assays.

Published: July 17, 2018 Copyright: © 2018 Odame Anto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper. Funding: The authors received no specific funding for this work. Competing interests: The authors have declared that no competing interests exist.

Results Pregnancies of AMA had a significantly higher levels of sFlt-1, 8-epi-PGF2α and 8-epiPGF2α: PIGF ratio but a reduced level of PIGF, TAC and PIGF: sFlt-1 ratio compared to 20–29 years (p