Recombinant fragment of Human USP34 with tag: LALLLSVHTP KQLNPALIPT.
LQELLSKCRT CLQQRNSLQE QEAKERKTKD DEGATPIKRR RVSSDEEHTV.
Product datasheet
Anti-USP34 antibody ab68343 1 Abreviews
4 Images
Overview Product name
Anti-USP34 antibody
Description
Mouse monoclonal to USP34
Tested applications
Suitable for: WB, ICC/IF, IHC-P, ELISA
Species reactivity
Reacts with: Human Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog
Immunogen
Recombinant fragment of Human USP34 with tag: LALLLSVHTP KQLNPALIPT LQELLSKCRT CLQQRNSLQE QEAKERKTKD DEGATPIKRR RVSSDEEHTV DSCISDMKTE TREVLTPTST SDNETRDSSI I (NP_055524, 3296 a.a. ~ 3396 a.a). Run BLAST with
Positive control
Run BLAST with
HeLa cells. A549 cell lysate. Human seminoma.
Properties Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None Constituents: 1X PBS, pH 7.2
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Applications Our Abpromise guarantee covers the use of ab68343 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews
Notes
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 387 kDa. 1
Application Abreviews
Notes
ICC/IF
Use a concentration of 10 µg/ml.
IHC-P
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. (ie. Place sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes, cool sample subsequently).
ELISA
Use at an assay dependent concentration.
Target Function
Ubiquitin hydrolase that can remove conjugated ubiquitin from AXIN1 and AXIN2, thereby acting as a regulator of Wnt signaling pathway. Acts as an activator of the Wnt signaling pathway downstream of the beta-catenin destruction complex by deubiquitinating and stabilizing AXIN1 and AXIN2, leading to promote nuclear accumulation of AXIN1 and AXIN2 and positively regulate beta-catenin (CTNBB1)-mediated transcription. Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins.
Tissue specificity
Expressed in brain at low level.
Sequence similarities
Belongs to the peptidase C19 family.
Post-translational modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Images Immunoperoxidase of monoclonal antibody to USP34 ab68343 on formalin-fixed paraffinembedded human seminoma. Antibody concentration 3ug/ml.
Detection limit for recombinant tagged USP34 is approximately 1ng/ml as a capture antibody.
Sandwich ELISA - USP34 antibody (ab68343)
Anti-USP34 antibody (ab68343) at 1 µg/ml + immunogen at 0.1 µg
Secondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 387 kDa Western blot - USP34 antibody (ab68343)
Observed band size : 43 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards 3
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors