Anti-USP34 antibody - Abcam

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Recombinant fragment of Human USP34 with tag: LALLLSVHTP KQLNPALIPT. LQELLSKCRT CLQQRNSLQE QEAKERKTKD DEGATPIKRR RVSSDEEHTV.
Product datasheet

Anti-USP34 antibody ab68343 1 Abreviews

4 Images

Overview Product name

Anti-USP34 antibody

Description

Mouse monoclonal to USP34

Tested applications

Suitable for: WB, ICC/IF, IHC-P, ELISA

Species reactivity

Reacts with: Human Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog

Immunogen

Recombinant fragment of Human USP34 with tag: LALLLSVHTP KQLNPALIPT LQELLSKCRT CLQQRNSLQE QEAKERKTKD DEGATPIKRR RVSSDEEHTV DSCISDMKTE TREVLTPTST SDNETRDSSI I (NP_055524, 3296 a.a. ~ 3396 a.a). Run BLAST with

Positive control

Run BLAST with

HeLa cells. A549 cell lysate. Human seminoma.

Properties Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None Constituents: 1X PBS, pH 7.2

Purity

Protein A purified

Clonality

Monoclonal

Isotype

IgG2a

Light chain type

kappa

Applications Our Abpromise guarantee covers the use of ab68343 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews

Notes

WB

Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 387 kDa. 1

Application Abreviews

Notes

ICC/IF

Use a concentration of 10 µg/ml.

IHC-P

Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. (ie. Place sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes, cool sample subsequently).

ELISA

Use at an assay dependent concentration.

Target Function

Ubiquitin hydrolase that can remove conjugated ubiquitin from AXIN1 and AXIN2, thereby acting as a regulator of Wnt signaling pathway. Acts as an activator of the Wnt signaling pathway downstream of the beta-catenin destruction complex by deubiquitinating and stabilizing AXIN1 and AXIN2, leading to promote nuclear accumulation of AXIN1 and AXIN2 and positively regulate beta-catenin (CTNBB1)-mediated transcription. Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins.

Tissue specificity

Expressed in brain at low level.

Sequence similarities

Belongs to the peptidase C19 family.

Post-translational modifications

Phosphorylated upon DNA damage, probably by ATM or ATR.

Images Immunoperoxidase of monoclonal antibody to USP34 ab68343 on formalin-fixed paraffinembedded human seminoma. Antibody concentration 3ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) - USP34 antibody (ab68343)

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Immunofluorescence of monoclonal antibody to USP34 ab68343 on HeLa cells. Antibody concentration 10 ug/ml.

Immunocytochemistry/ Immunofluorescence USP34 antibody (ab68343)

Detection limit for recombinant tagged USP34 is approximately 1ng/ml as a capture antibody.

Sandwich ELISA - USP34 antibody (ab68343)

Anti-USP34 antibody (ab68343) at 1 µg/ml + immunogen at 0.1 µg

Secondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size : 387 kDa Western blot - USP34 antibody (ab68343)

Observed band size : 43 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards 3

If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team.

Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors

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