significant association between A allele at -1490 and T allele at -1461 of. PRDX6 gene and asthma, especially when haplotyped with AT/AT. S198 Abstracts.
S198 Abstracts
Correlation between Exhaled Nitric Oxide (eNO) and Symptoms, Medication Use and Quality of Life in Patients Diagnosed with Mild to Moderate Persistent Asthma J. Kaczmarek, M. Bochenska-Marciniak, ´ M. Kupczyk, P. Górski, P. Kuna; Department of Pneumonology and Allergy, Medical University of Lodz, Lodz, POLAND. RATIONALE: Nitric oxide has been suggested as one of the markers of chronic inflammation in the airways which can be used for non-invasive asthma diagnosis. The aims of the study were to find the possible correlation between the eNO levels and quality of life, symptom score, medication use, morning PEF in patients with mild to moderate persistent asthma. METHODS: The data about medical history, basic spirometry (FEV1, FVC, PEF, FEV1/FVC) and reversibility test of 120 patients referred to the Outpatient Clinic of the Div of Pneumonology and Allergy with symptoms suggesting mild to moderate asthma were collected. During 14 days of the study phase patients received only short acting agonist - salbutamol and monitored the morning PEF, symptoms and rescue medication use, eNO level was measured using NIOX and standard technique and AQOLQ (Juniper) were filled in. RESULTS: 84 patients, mean age 36,39±12,5 years, 36 males, with mean asthma duration 7,36±8,02 years, with mean FEV1 2,99±0,88 l/s, % of predicted value 82,09±7,42 and reversibility 20,83±5,14 (% of dilatation) completed the study. Mean eNO value was 34,35±31,52 ppb. Mean morning PEF 421,39±135,74 l/min, mean number of inhalations of rescue medication 3,89±1,3 per day, mean asthma symptom score 1,51±0,56 per day and mean score of AQOLQ 4,63±0,84. We did not find correlation neither between eNO and AQOLQ mean value (r=-0,05 p=0,626) nor between eNO and morning PEF (r=0,012 p=0,9), rescue medication inhalation number (r=-0,19 p=0,08) and symptoms (r=0,024 p=0,82). CONCLUSIONS: Further trials are necessary to study the eNO usefulness in diagnosing and monitoring chronic asthma. Funding: Medical University of Lodz
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Sex Differences in Effects of Breastfeeding on Asthma and Allergy in Relation to Parental Atopy P. J. Mandhane, J. M. Greene, M. R. Sears; McMaster University, Hamilton, ON, CANADA. RATIONALE: The influence of breast-feeding on developing asthma and atopy may differ between males and females with and without parental atopy. METHODS: A New Zealand birth cohort of 1037 children was followed to age 26. We examined asthma (at 9 years) and atopy (at 13 years) in males and females in relation to maternal atopy, paternal atopy and breastfeeding. Interactions were explored in logistic regression models. RESULTS: For male children, breast-feeding (OR 3.36, 95%CI 2.18 4.53) and maternal atopy (OR 4.14, 95%CI 2.83 - 5.46) but not paternal atopy (OR 2.23, 95%CI 0.48 - 3.98) were significantly associated with asthma. Breast-feeding increased the risk for asthma among males with paternal atopy (OR 3.79, 95%CI 2.13 - 5.46) compared with non-breastfed males with paternal atopy, but did not further increase risk among those with maternal atopy. For female children, breast-feeding (OR 2.09, 95%CI 0.98 - 3.20) was associated with asthma, but neither maternal atopy (OR 0.47, 95%CI 0.00 - 2.64) nor paternal history (OR 0.85, 95%CI 0.00 - 3.04) were independent risk factors. However, breast feeding markedly increased the risk for asthma in females with maternal atopy (OR 14.67, 95%CI 12.57 - 16.77) compared with non-breast-fed females with maternal atopy, but not among those with paternal atopy. A similar pattern of results were obtained for the outcome of atopy at age 13. CONCLUSIONS: Maternal and paternal histories of atopy affect male and female children differently with respect to development of asthma and atopy. Breast-feeding influences these relationships in a sex-specific manner. Funding: Health Research Council of New Zealand
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J ALLERGY CLIN IMMUNOL FEBRUARY 2006
The Follow of Changes in Inflammatory Markers in Induced Sputum of Patients with Bronchial Asthma and Allergic Rhinitis in Relation to Exposure to Pollen Allergens V. Hrasko, I. Malkusova, J. Hanzlikova, P. Panzner; Medical Faculty Hospital, Plzen, CZECH REPUBLIC. RATIONALE: We intended to verify the hypothesis, that eosinophilic inflammation in the lower airways is present not only in patients with asthma but also in patients with seasonal rhinitis without asthma. METHODS: Sputum was induced and processed according to Pizichini. We evaluated changes in cell counts (eosinophils, macrophages, neutrophils) and humoral marker (ECP). Cell count was evaluated by means of Hemacolor (DCC) and immunocytochemistry (ICC), ECP in supernatant by means of enzymoimmunoassay. RESULTS: Sputum ECP levels before the pollen season in patients with asthma were higher compared to patients with rhinitis. The levels of ECP in patients with asthma and rhinitis during the pollen season were elevated in comparison to the results before the season. ECP levels after the season in patients with asthma were higher compared to patients with rhinitis. Sputum eosinophil counts (both by DCC and ICC) before the pollen season patients with asthma showed tendency to higher values compared to rhinitis patients and control group. During the season the tendency to higher numbers of sputum eosinophils was seen both in patients with asthma and patients with rhinitis. Eosinophil counts after the season in patients with asthma showed again tendency to higher values compared to patients with rhinitis and control group. CONCLUSIONS: Differences in sputum ECP levels and eosinophil counts in patients with asthma before, during and after the pollen season were expected, it corresponds with persistent inflammation of lower airways. Elevated ECP levels in rhinitis patients during the pollen season indicate persistent inflammation in spite of absence of asthma symptoms. Funding: VZ 111006/MSM0021620812
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MONDAY
Association between Peroxiredoxin (PRDX) VI Gene Polymorphism and Asthma and Its Intermediate Phenotypes T. B. Kim, Y. S. Cho, C. S. Park, K. Y. Lee, H. B. Moon; Department of Medicine, Clinical Research Center for Chronic Obstructive Airway Disease, Asan Medical Center, University of Ulsan College of Medicine, Seoul, REPUBLIC OF KOREA. RATIONALE: It has been suggested that the oxidative stress may play an important role in the pathogenesis of asthma. We performed this study to evaluate the association between the genetic variation of PRDX VI and the development of asthma and its intermediate phenotypes, e.g. atopy and airway hyperresponsiveness in Korean general population. METHODS: A total of 2,863 subjects aged from 10 to 18 years living in a rural area were recruited. Questionnaire reporting regarding current wheeze and nocturnal cough, methacholine bronchial provocation test, skin prick test to common aeroallergens, and measurement of serum total IgE were performed. The single nucleotide polymorphisms (SNPs) of PRDX VI gene were analyzed by high throughput single base pair extension method. RESULTS: Two informative SNPs out of total 7 were detected in the PRDX VI gene. One was a novel SNP at -1490A>G, with the allele frequency of 56.6% for AA, 35.7% for AG, and 7.4% for GG respectively. The other was at -1461C>T, with 31.2% for CC, 47.5% for CT, and 20.7% for TT, respectively. Significant associations were found between the -1461C>T and asthma [p=0.038, OR (95% CI) =1.82 (1.026-3.239)], between the -1490A>G and nonatopic asthma [p=0.028, OR (95% CI) = 4.26 (1.491-12.176)], and AT/AT haplotype with asthma [p=0.035, OR (95% CI) =1.839 (1.035-3.268)]. CONCLUSIONS: We discovered a novel SNP at -1490A>G of PRDX6 gene in Korean general population. These data suggest that there is a significant association between A allele at -1490 and T allele at -1461 of PRDX6 gene and asthma, especially when haplotyped with AT/AT.
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