ATTI XLII Congresso SIBM

Vol. 18 - fasc. 1 - 2011

ATTI XLII Congresso SIBM OLBIA 23-28 Maggio 2011

Biologia Marina Mediterranea (Biol. Mar. Mediterr. / ISSN 1123-4245) Direttore Responsabile/Editor in chief Giulio Relini, Univ. di Genova – e-mail: [email protected]

Editori Associati/Associate Editors GIACCONE G., Univ. di Catania - e-mail: [email protected] GUIDETTI P., Univ. di Lecce - e-mail: [email protected] OCCHIPINTI A., Univ. di Pavia - e-mail: [email protected] PRONZATO R., Univ. di Genova - e-mail: [email protected] Comitato di Redazione/Editorial Advisory Board

BELLAN Gérard, (Marseille, France) BELLAN SANTINI Denise (Marseille, France) BELLUSCIO Andrea (Roma, Italy) BERTRAND Jacques (Nantes, France) BORTONE Stephen (Tampa, USA) DAVENPORT John (Cork, Ireland) GAMBI Maria Cristina (Napoli, Italy) GENOVESE Lucrezia (Messina, Italy) HALLEGRAEFF Gustaaf M. (Hobart, Australia) HAWKINS Stephen J. (Bangor, U.K.)

JENSEN Antony (Southampton, U.K.) KALLIANIOTIS Argyris (Kavala, Greece) NEVES dos SANTOS Miguel (Olhão, Portugal) PAPACONSTANTINOU Costas (Athens, Greece) RYLAND John (Swansea, U.K.) SCHEMBRI Patrick (Msida, Malta) SEAMAN William (Gainesville, USA) SERENA Fabrizio (Livorno, Italy) SOCAL Giorgio (Venezia, Italy)

Segreteria di Redazione/Editorial Assistants E. Massaro, S. Queirolo, R. Simoni Redazione/Editorial Office S.I.B.M. c/o Dip.Te.Ris., University of Genova, Viale Benedetto XV, 3 – 16132 Genoa, Italy Tel. e fax 0039 010 357888 e-mail: [email protected] Editore Stampatore/Production Editor/Publisher Erredi Grafiche Editoriali S.n.c., Via Trensasco, 11 – 16138 Genova Abbonamenti ed arretrati/Subscription and back issues Erredi Grafiche Editoriali S.n.c. tel. 0039 010 8356880 e-mail: [email protected] Biologia Marina Mediterranea è la rivista ufficiale della Società Italiana di Biologia Marina e viene repertoriata da ASFA e da Zoological Record. Biologia Marina Mediterranea is a journal of Italian Society for Marine Biology and is currently indexed and abstracted by ASFA and Zoological Record. Biologia Marina Mediterranea è una rivista che tratta ogni aspetto della biologia marina. Al momento pubblica solo gli atti di congressi e simposi organizzati dalla SIBM, o sintesi su argomenti specifici. Come supplementi della rivista sono pubblicati anche manuali e guide. Tutti i lavori sono sottoposti a qualificati referee nazionali ed internazionali. Biologia Marina Mediterranea is an international journal dealing with all aspects of marine biology. At present only proceedings of workshops and congresses organized by SIBM or synthesis on special subjects are published. Guides and manuals are printed as supplements of the Journal. All papers are rigorously peer reviewed by national and international experts.

Periodico della S.I.B.M. edito dalla Erredi Grafiche Editoriali, Genova – Autorizzazione Tribunale di Genova n. 34/94 del 13 dicembre 1994

ATTI

del XLII Congresso della

SOCIETÀ ITALIANA DI BIOLOGIA MARINA

OLBIA (OT) 23 - 28 maggio 2011

Ente Organizzatore Consorzio di Gestione Area Marina Protetta di tavolara punta coda cavallo ministero dell’ambiente e della tutela del territorio e del mare

COMITATO ORGANIZZATORE Augusto NAVONE Sarah CARONNI Graziella DEDOLA Giovanna SPANO Gavino CANU Pieraugusto PANZALIS Stanislao LEDDA Francesco PIRAS Salvatore VITALE Giulia CECCHERELLI Andrea COSSU SEGRETERIE ORGANIZZATIVE Segreteria Tecnica SIBM c/o DIP.TE.RIS. Università di Genova Viale Benedetto XV, 3 16132 GENOVA Tel. e fax 010 357888 e-mail: [email protected] Consorzio di gestione dell’Area Marina Protetta Tavolara Punta Coda Cavallo Via Dante, 1 07026 Olbia (OT) Tel. 0789 203013 Fax 0789 204514 e-mail: [email protected]

Le opinioni espresse negli articoli non necessariamente rappresentano quelle ufficiali della S.I.B.M.

Biol. Mar. Mediterr. (2011), 18 (1): 274-275

V. Pasquale1, M. Guida2, P. Cennamo3, V. Mastascusa1,4, M. Greco1, R. Sandulli1 Department of Environmental Sciences, “Parthenope” University, Naples, Italy. [email protected] Dept. of Biological Science, Section Physiology and Hygiene, “Federico II” University, Naples, Italy. 3 Faculty of Letters, University “Suor Orsola Benincasa”, Naples, Italy. 4 Department of Applied Sciences, “Parthenope” University, Naples, Italy. 1

2

CULTIVABLE HETEROTROPHIC BACTERIA ASSOCIATED TO CORALLIUM RUBRUM BATTERI ETEROTROFI COLTIVABILI ASSOCIATI A CORALLIUM RUBRUM Abstract - In our study some of the most representative components of the heterotrophic bacterial communities colonizing the surfaces of Corallium rubrum were isolated and identified. Among these, Actinomyces sp., Bacillus licheniformis, B. megaterium, B. mycoides, B. pumilus, Bacillus sp., Kocuria palustris, Kocuria sp., Pseudoalteromonas sp., Pseudomonas stutzeri, Pseudomonas sp., Staphylococcus equorum, Vibrio harvey, V. hepatarius and V. shilonii are the most abundant cultivable heterothrophic bacteria adherent to the surfaces of C. rubrum sampled. In particular, we identified the same species of Vibrionaceae that were considered the main cause of disease in other species of corals in previous studies. Key-words: Corallium rubrum, cultivable heterotrophic bacteria, Vibrio, Bacillus, Pseudomonas.

Introduction - Corals, including the Mediterranean red coral (Corallium rubrum L 1758) are associated with a multitude of different microorganisms (bacteria, algae, etc.) colonizing their surface mucus layer, tissue and calcium carbonate skeleton (Rosenberg et al., 2007). Koren and Rosenberg (2006) described several bacteria belonging to the family of Vibrionaceae associated to the hexacoral Oculina patagonica. However, some bacterial species, particularly Vibrio corallilyticus, V. harveyi, V. splendidus, and V. shilonii, have been recognized as a possible cause of some coral diseases, such as coral bleaching (Kushmaro et al., 1996; Vezzulli et al., 2010). To our knowledge, the ecological relationships between microorganisms and C. rubrum are still unknown. The aim of present study was to investigate the Cultivable Heterotrophic Bacteria (CHB) associated with the coenosarc of C. rubrum. Materials and methods - In June 2010, several colonies of C. rubrum were collected from seabeds surrounding Procida Island (Naples) during ROV divings. The surfaces of five selected colonies of red coral were washed with sterile saline solution. Surface and coral tissue samples were taken by sterile cotton swabs from coral surface of ca. 10 cm2, transferred to 2 mL sterile tubes with saline solution, and stored at 8-10 °C. The bacteria were suspended in saline solution by vortexing. After serial dilution (1:10), CHB were isolated by pour plate techniques in R2A agar (Oxoid, UK) supplemented with 1% NaCl. Plates were incubated, aerobically, at 20 °C until 10 days. CHB were expressed as CFU/cm 2 of coral surface including mucus and tissue. The identification of the isolates was accomplished by molecular techniques. DNA was extracted using a procedure described by Doyle and Doyle (1987). PCR amplification was carried out using the primer set for 16S rRNA (Diez et al., 2001). The sequences obtained were compared with available sequences in the GeneBank database. Results - Microbiological analysis showed an unequal concentration of the CHB associated with the surfaces (mucus and tissue) of the different samples of C. rubrum. The bacterial concentrations ranged between 2 and 3.4∙105 CFU/cm2 (Tab. 1). A bacterial concentration higher than 104 CFU/cm2 was only detected in sample 5, while samples 1, 2, and 3 had a very low bacterial density (