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JBC Papers in Press. Published on August 9, 2006 as Manuscript M605394200 The latest version is at http://www.jbc.org/cgi/doi/10.1074/jbc.M605394200

BISANDROGRAPHOLIDE FROM ANDROGRAPHIS PANICULATA ACTIVATES TRPV4 CHANNELS* Paula L. Smith1, Katherine N. Maloney2, Randy G. Pothen1, Jon Clardy2, David E. Clapham1 From the (1) Howard Hughes Medical Institute, Department of Cardiology, Children’s Hospital, Boston, Massachusetts 02115 and (2) Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115 Running Title: Bisandrographolide Activates TRPV4 Address correspondence to: David E. Clapham, Howard Hughes Medical Institute, Department of Cardiology, Children’s Hospital, 1309 Enders Research Building, 320 Longwood Avenue, Boston, Massachusetts 02115; Tel. 617-919-2680; Fax. 617-731-0787; E-Mail: [email protected]

INTRODUCTION TRPV4a is a member of the transient receptor potential superfamily of ion channels. Presumably, the assembly of four polypeptide subunits into a pore permeable to cations forms TRP channels. The pore opens and closes (gates) to allow cations to cross the membrane, and gating is usually controlled by one or more stimuli (e.g., ligand binding). Many TRP channels including TRPV4 are involved in sensing properties of the extracellular or intracellular environment (e.g., temperature, acidity, osmolarity, etc.). These characteristics provide a general description of TRP channels; however, a universal function for the TRP channel superfamily has yet to be found. TRPV4 is one of six members of the vanilloid TRP subfamily; the first member of this group of channels is TRPV1, a channel gated by multiple stimuli including capsaicin, protons, heat and anandamide (1-3). TRPV4 is most closely related to TRPV1, TRPV2 and TRPV3 as determined by sequence homology. In addition these four channels are functionally similar because they are all activated by increases in temperature. TRPV5 and TRPV6 are notably different from the other four TRPV channels as determined by sequence homology and channel function; both TRPV5 and TRPV6 are highly

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possibility that activation of TRPV4 by BAA could play a role in some of the effects of Andrographis extract described in traditional medicine.

Many transient receptor potential channels (TRP) are activated or blocked by various compounds found in plants; two prominent examples include the activation of TRPV1 channels by capsaicin and the activation of TRPM8 channels by menthol. We sought to identify additional plant compounds that are active on other types of TRP channels. We screened a library of extracts from fifty Chinese herbal plants using a calcium-imaging assay to find compounds active on TRPV3 and TRPV4 channels. An extract from the plant Andrographis paniculata potently activated TRPV4 channels. The extract was fractionated further, and the active compound was identified as bisandrographolide A (BAA). We used purified compound to characterize the activity of BAA on certain TRPV channel subtypes. Although BAA activated TRPV4 channels with an EC50 of 790-950 nM, it did not activate or block activation of TRPV1, TRPV2 or TRPV3 channels. BAA activated a large TRPV4-like current in immortalized mouse keratinocytes (308 cells) that have been shown to express TRPV4 protein endogenously. This compound also activated TRPV4 currents in cell-free outside-out patches from HEK293T cells overexpressing TRPV4 cDNA suggesting that BAA can activate the channel in a membrane delimited manner. Another related compound, andrographolide, found in abundance in the plant Andrographis was unable to activate or block activation of TRPV4 channels. These experiments show that BAA activates TRPV4 channels, and we discuss the

calcium selective in contrast to most other TRP channels (4,5). Similar to TRPV1, TRPV4 can be activated by a wide range of stimuli including low osmolarity solutions, heating to warm temperatures, metabolites of arachidonic acid (epoxyeicosatrienoic acids or EETs) and 4-αphorbol-12,13-didecanoate (4αPDD), a synthetic phorbol ester often used as a negative control for phorbol-12,13-didecanoate (6-10). The channel is widely expressed and can be found in kidney, skin, brain, lung, smooth muscle, vascular endothelium, liver and a number of other areas (4). Functional studies, channel localization and analysis of TRPV4 knockout mice, suggest that the channel may play a role in osmosensation, nociception, and heat sensation; however, the mechanistic details about these roles are still being investigated (11-15). Plant compounds activate several TRP channels. For example, TRPV1 is activated by capsaicin, the ‘hot’ ingredient in chili peppers; TRPV3 is activated by camphor from an evergreen tree, carvacrol in oregano and eugenol in thyme (16,17); TRPM8 is activated by menthol, the cooling compound in peppermint (18,19), and TRPA1 is activated by allyl isothiocyanate, the pungent compound in mustard oil (20). These are several prominent examples, but other compounds with activity on TRP channels also have been described. To search for novel compounds active on TRP channels, we used the Starr collection, a library of prefractionated extracts of fifty plants used in traditional Chinese medicine (TCM), which was assembled by the Osher Institute at Harvard Medical School. We used a calciumimaging assay to screen the library for activity on TRPV3 and TRPV4 channels. Hits from the calcium-imaging screen were analyzed further in an electrophysiology assay. Extracts displaying activity in the electrophysiology assay were purified, chemically analyzed and tested to identify the active compound in the extract. We show that a compound called bisandrographolide A (BAA) contained in extracts of the plant Andrographis paniculata activates TRPV4 channels. We speculate about the possibility that activation of TRPV4 by BAA might play a role in some of the reported effects of Andrographis extract in traditional medicine.

EXPERIMENTAL PROCEDURES

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Cell culture - HEK293T cells were maintained at 37 °C in media containing 90% DMEM/Ham’s F12 (1:1), 10% fetal bovine serum, 100 U/mL penicillin and 100 µg/mL streptomycin in 5% CO2. 308 cells from a mouse keratinocyte cell line were maintained at 37 °C in media containing 90% DMEM, 10% fetal bovine serum, 100 U/mL penicillin and 100 µg/mL streptomycin in 5% CO2. We received the 308 keratinocytes from S.H. Yuspa at the National Cancer Institute; this cell line is a papilloma-derived keratinocyte cell line from adult BALB/c mouse skin (21). Calcium imaging - 35 mm dishes of HEK293T cells were transiently transfected with 3 µg of either human TRPV3 or mouse TRPV4 cDNA and 0.25 µg of dsRed2 cDNA (Clontech) using Lipofectamine 2000 (Invitrogen). After 24 hours transfected cells were subcultured into 96 well plates. 48-72 hours after transfection cells were loaded with the calcium indicator dye fluo-4. 5 µM fluo-4, AM (acetoxymethyl ester, Molecular Probes) and 0.2% Pluronic F-127 (Molecular Probes) dissolved in Opti-mem (Invitrogen) were added to cells and incubated at 37 °C in 5% CO2 for 45 minutes. Cells were rinsed twice with a standard extracellular saline solution (135 mM NaCl, 5 mM KCl, 2 mM CaCl2, 1 mM MgCl2, 10 mM HEPES and 10 mM glucose) and allowed to incubate at room temperature for 15-30 minutes. Calcium imaging was performed on an Olympus microscope with a cooled Hamamatsu CCD camera. 10-50 transfected cells in the field were selected by red fluorescence, and average fluo-4 fluorescence was measured in these cells over time. Images were recorded at 3-second intervals. Cells that did not remain in the field for the duration of the experiment were eliminated from analysis. The first five points in the experiment were averaged to find the baseline fluorescence (Fbaseline), and ∆F/F ((Ft-Fbaseline)/Fbaseline) was calculated for each cell at all time points. Average ∆F/F of all cells in the field was calculated and plotted versus time. Error bars ± SEM. Electrophysiology - 35 mm dishes of HEK293T cells were transiently transfected with 2 µg (Figures 1 and 5) or 3 µg (Figure 4) of mouse TRPV4 cDNA or 1 µg of rat TRPV1, rat TRPV2

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was not feasible because the quantity of BAA was limited. For these experiments we diluted BAA to the indicated concentration and added 500 µL of solution directly to the bath; bath volume was ~250 µL. Plant Material - Fifty herbs widely used in TCM were selected and obtained by Dr. David Eisenberg and his colleagues at the Osher Institute at Harvard Medical School. Extraction and Prefractionation - Extraction and prefractionation steps were performed at the Instituto Nacional de Biodiversidad (INBio) in Costa Rica. Gram quantities of each of the Chinese medicinal plants were extracted using methanol with grinding. The extract from each plant was evaporated in a minimum volume of methanol and adsorbed onto Diaion HP20 (Mitsubishi Chemical). The resin was washed with deionized water and extracted with ethanol. This solution was adjusted to 20% aqueous ethanol and loaded onto a 500 mL RP-C18 column, where it was separated into 48 fractions. Fractions of sufficient weight, as judged by evaporative light scattering detection, were evaporated, reconstituted in DMSO, arrayed in 96-well plates, and shipped to the Institute of Chemistry and Cell Biology-Longwood (ICCB-L) at Harvard Medical School for integration into their screening platform. Isolation and characterization of BAA - The active fraction from Andrographis was separated by HPLC on an Agilent 1100 chromatograph (Agilent Technologies) using a semi-preparative Discovery HS-C18 column (Supelco, 250 x 10 mm, 5 mm particle size) with an acetonitrile-water gradient to afford 0.4 mg of pure, active compound. 1D 1H and 2D (double quantum filtered 1 1 H- H COSY (dqfCOSY), 1H-13C HMQC, 1H-13C HMBC, and NOESY) 2D (double quantum filtered 1H-1H COSY (dqfCOSY), 1H-13C HMQC, and NOESY) NMR spectra were obtained on a Varian Oxford NMR AS500 spectrometer with standard pulse sequences operating at 500 MHz. CD3OD was used as solvent. Offline processing was conducted using Mestre-C NMR Software (Mestrelab Research). The NMR data revealed a diterpene dimer with the bisandrographolide carbon skeleton shown in Figure 1E. 1H chemical shifts were identical to literature values for BAA A (22) (Supplementary Figure 1 and

or human TRPV3 (Figure 2) using Lipofectamine 2000. Channel DNA was cotransfected with 0.25 µg of either eGFP or dsRed2 cDNA as a marker for transfection. After 6-12 hours transfected cells were subcultured onto glass coverslips, and recordings made 24-72 hours after transfection. We visualized eGFP or dsRed2-positive cells with a fluorescence microscope (Olympus, 40x, N.A. 0.9) and recorded currents using an Axopatch 200B amplifier and pClamp8 software (Axon Instruments). During voltage ramps, currents were sampled at 10 kHz and the recordings were filtered at 2 kHz. For all experiments the membrane potential was held at -60 mV. Borosilicate glass pipettes with resistances of 2-4 MΩ were used for recording. For recording HEK293T cells, the bath solution contained 135 mM NaCl, 5 mM CsCl, 2 mM CaCl2, 1 mM MgCl2, 10 mM HEPES, 10 mM glucose, pH 7.4. For recording 308 cells we used a low chloride bath solution containing 135 mM sodium gluconate, 10 mM NaCl, 5 mM cesium methanesulfonate (CsMES), 2 mM calcium gluconate, 1 mM MgSO4, 10 mM HEPES, 10 mM glucose, pH 7.4. For recording cell-free patches we used a nominally Ca2+-free external solution containing 135 mM NaCl, 5 mM CsCl, 1 mM MgCl2, 10 mM HEPES, 10 mM glucose, pH 7.4. In all recordings measuring TRPV4 currents the pipette solution contained 100 mM CsMES, 10 mM CsCl, 3 mM MgATP, 0.2 mM NaGTP, 10 mM Cs4BAPTA, 80 µM CaCl2, 10 HEPES, 20 mM mannitol; pH 7.3. Free [Ca2+] in the pipette solution was ~1 nM as calculated by MaxChelator. For measurement of TRPV1, TRPV2 or TRPV3 currents the pipette solution contained 100 mM CsMES, 4 mM CsCl, 3 mM MgATP, 0.2 mM NaGTP, 10 mM Cs4BAPTA, 3.2 mM CaCl2, 10 HEPES, 20 mM mannitol; pH 7.3. Free [Ca2+] in the pipette solution was ~100 nM as calculated by MaxChelator. Junction potentials were corrected in all experiments. Reagents - Fraction H11 (from the Starr collection), BAA, 4αPDD (Calbiochem), andrographolide (Calbiochem), capsaicin (Sigma), 2-aminoethoxydiphenyl borate (2-APB, Calbiochem) were dissolved in DMSO at high concentrations then diluted in the appropriate extracellular buffer before addition to the bath. Final DMSO concentrations were 0.1-0.5%. Continuous perfusion during BAA experiments

RESULTS We screened a library of extracts from fifty plants used in TCM using a Ca2+ imaging assay. The assay could detect compounds that opened or blocked TRPV3 channels and those that opened TRPV4 channels. Approximately 10 µg/mL of each extract was added to HEK293T cells transfected with either TRPV3 or TRPV4 cDNA and loaded with the Ca2+ indicator fluo-4. Hits from the Ca2+ imaging screen were tested further in an electrophysiological assay to verify activity. Although one extract displayed blocking activity for TRPV3 in the Ca2+ imaging assay, the effect could not be repeated in the electrophysiology assay. Several fractions 2+ stimulated Ca influx in TRPV4 transfected cells (Supplementary Figure 2). In the electrophysiology assay 10 µg/mL of Fraction H11 from the Starr collection caused activation of a large current in HEK293T cells overexpressing TRPV4 channels (Figures 1A and 1B). NMR analysis showed that Fraction H11 contained several compounds; H11 was separated further by reverse phase HPLC, and the fractions were tested in the electrophysiology assay to identify a single active compound. This compound was identified as bisandrographolide A (BAA) by NMR and mass spectrometry (Figure 1E). No other compounds in Fraction H11 activated TRPV4 current. We applied purified BAA to TRPV4expressing cells to confirm that the compound activated TRPV4 channels similar to Fraction H11 (Figures 1C and 1D). We also obtained a dose response curve for activation of TRPV4 channels by BAA. Figure 1F shows average maximum inward current density measured at -110 mV plotted against concentration of BAA; data are fitted with a Hill equation (EC50 = 950 nM BAA, Hill coefficient = 1.4). A dose response curve plotting average I/Imax (current normalized to the maximum inward current measured at -110 mV in 4


each experiment) against concentration of BAA yielded a similar result: EC50 = 790 nM BAA and Hill coefficient = 1.8 (data not shown). To determine if the activity of BAA was specific for TRPV4, we applied BAA to other closely related vanilloid TRP channels. As shown in Figure 2 BAA did not activate TRPV1, TRPV2 or TRPV3 channels overexpressed in HEK293T cells even though these channels were robustly activated by known agonists (100 nM capsaicin, 500 µM 2-aminoethoxydiphenyl borate (2-APB) or 100 µM 2-APB, respectively, n=5 for each experiment). In addition, we tested whether the compound could block activation of TRPV1, TRPV2 or TRPV3 channels; however, we did not observe block after application of 5 µM BAA (data not shown). We did not test other TRP channel subtypes. To test whether BAA activates endogenously expressed TRPV4 channels similar to heterologously expressed channels, we investigated currents in 308 cells, a mouse keratinocyte cell line previously shown to express both TRPV3 and TRPV4 channels (23). We could stimulate large TRPV4 currents after addition of 1 or 10 µM 4αPDD in approximately half of the cells we recorded (n=7 of 16 cells); current activated by 10 µM 4αPDD in a 308 cell is shown in Figure 3A. All recordings from 308 cells were performed in a low chloride bath solution to decrease the magnitude of a very large Ca2+activated chloride current that was activated as a result of Ca2+ influx through open TRPV4 channels. The 4αPDD-activated current most likely resulted from opening of homomeric TRPV4 channels. Western blots (not shown) confirmed a previous study demonstrating that TRPV4 and TRPV3 subunits do not coassemble to form heteromeric channels (24; see also 23). Similar to the action of 4αPDD, when 5 µM BAA was applied to 308 cells, large TRPV4 currents were recorded in approximately half of the cells (Figure 3B, n=6 of 11 cells). Although the time to peak current shown in the figure was rather slow, the current begins to activate shortly after application of BAA. Also, the time to peak TRPV4 current varied a great deal from cell to cell even when measured from heterologously expressed TRPV4 channels. We note that the reversal potential of the current activated by BAA

Supplementary Table I). LCMS of the active compound was obtained using a Micromass Platform LC-Z spectrometer, equipped with a Waters 2690 LC system and Waters 2690 photodiode array detector, and processed using MassLynx software (Waters Corporation), and revealed a molecular ion [M+1]+ of 665.53, consistent with BAA.

DISCUSSION

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In this study we have identified a novel activator of TRPV4 channels from the plant Andrographis paniculata used in traditional medicine in many regions of Asia. The compound, bisandrographolide A, has an EC50 of approximately 790 - 950 nM for TRPV4 activation and is not active on other closely related members of the vanilloid TRP subfamily (TRPV1, TRPV2 and TRPV3) at concentrations that fully activate TRPV4 channels. We showed that BAA activated endogenous TRPV4 channels in keratinocytes. We also demonstrated that BAA activated TRPV4 channels in cell free outside-out patches, suggesting that activation is membrane-delimited and not dependent on diffusible internal factors. Furthermore, we tested the activity of the monomer andrographolide on TRPV channels, and found that andrographolide neither activated nor blocked TRPV1, V2, V3 or V4 channels. TRPV4 is a nonselective cation channel that can be activated by a wide range of stimuli, like several other transient receptor potential channels. Activation causes opening of a pore that allows Na+, K+ and Ca2+ ions to cross the membrane. The resulting increases in internal free [Ca2+] and changes in transmembrane voltage potentially can affect cell signaling. TRPV4 has been implicated in osmoregulation, nociception, regulation of vascular tone, and heat sensation. The plant Andrographis paniculata is used in traditional medicine in various parts of Asia for a wide array of ailments. Extracts are typically used as an anti-inflammatory agent or immunostimulant. Indications include upper respiratory tract infections, diarrhea, fever, tonsillitis, snakebite, and many others (26). How the extract is used seems to vary widely depending on the region. Extracts of Andrographis are used as an herbal remedy for the common cold in Sweden where pills with a standardized amount of Andrographis extract and another herb (Eleutherococcus senticosus) are made and sold under the name Kan Jang. Due to the wide spread use of Andrographis extract in traditional medicine, there have been a relatively large number of studies investigating the effects of Andrographis extract as well as compounds purified from the extract. Many papers have focused on andrographolide, the most abundant compound in Andrographis. In one study andrographolide was shown to inhibit

is shifted slightly to the left of 0 mV. We believe this results from residual chloride current activated by Ca2+ entering through TRPV4 channels during prolonged activation. These results support the hypothesis that BAA is an effective activator of endogenous TRPV4 channels. To determine whether this compound activates TRPV4 in a membrane-delimited manner we applied 5 µM BAA to cell-free outside-out patches excised from HEK293T cells overexpressing TRPV4 cDNA (Figure 4). These experiments were performed in an external solution with no added Ca2+ in order to slow inactivation of the current. 5 µM BAA can activate a substantial current in excised patches with properties similar to whole cell currents under similar conditions. The reversal potential, rectification of the current through the channel, and the time course of activation are similar to TRPV4 current measured in whole cells bathed in nominally Ca2+ free external solution. We note that the reversal potential of the current in Figure 4A is also shifted to the left of 0 mV. In this instance we attribute the shift to the lack of external Ca2+. The ability of BAA to activate TRPV4 in patches suggests that the action of BAA does not require signaling through soluble internal factors. The most abundant compound isolated from Andrographis paniculata is andrographolide (Figure 5A), the monomer of BAA. Andrographolide has been shown previously to block NF-κB activation with an IC50 of approximately 10 µM and to display antiinflammatory properties in mouse models of inflammation (25). We applied high concentrations of andrographolide to TRPV4 expressing cells to determine if andrographolide has activity similar to BAA. However, application of 100 µM to 1 mM andrographolide did not activate TRPV4 channels although a large TRPV4 current could be elicited by the agonist 4αPDD (Figures 5B and 5C). High concentrations of andrographolide also did not activate or block activation of TRPV1, TRPV2 or TRPV3 channels (data not shown).

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experiments from Earley and colleagues support the hypothesis that Ca2+ influx through TRPV4 channels in arterial smooth muscle cells increases the number of Ca2+ sparks that activate largeconductance Ca2+-activated K+ channels, and the resulting increase in potassium current causes hyperpolarization and arterial dilation. Andrographis extract containing BAA presumably could act on vascular smooth muscle by the same mechanism to cause arterial dilation and a decrease in blood pressure. According to a database of information on compounds found in plants used in TCM (26) Andrographis also is used to treat snake bite and eczema; two conditions relating to the skin. Unfortunately there is little research on the efficacy of Andrographis extracts for treating these conditions. There are, however, some hints that TRPV4 might play a role in skin function. TRPV4 is highly expressed in skin, particularly in keratinocytes (10,33,34). As shown previously as well as in these experiments, very large currents can be elicited in keratinocytes by TRPV4 agonists (35). The Ca2+ influx through TRPV4 channels could play a role in skin function since it is known that Ca2+ signaling plays an important role in the proliferation and terminal differentiation of keratinocytes (36). In addition, there is some evidence that EET signaling may be involved in keratinocyte cornification (37). Although there is evidence that TRPV4 is present in mouse and human skin, we do not know enough about its functional role to speculate further on how activation of the channel could affect injury to the skin. These experiments show that TRPV4 is activated by BAA, a compound contained in extracts of the herbal medicine Andrographis paniculata, with an EC50 of 790-950 nM. Andrographis extract is used as a traditional medicine in various parts of Asia for a wide variety of illnesses. It is possible that activation of TRPV4 by BAA could play a role in some of the effects of Andrographis extract that have been reported previously.

NF-κB activation and reduce inflammatory response in a variety of mouse models (25). This mechanism may account for some of the antiinflammatory effects of Andrographis. In addition to andrographolide, many other compounds have been identified in Andrographis extract. BAA was first identified in 1994 in a screen of naturally occurring substances that induce differentiation of mouse myeloid leukemia (M1) cells (22). The authors identified 18 compounds found in methanol extracts of Andrographis paniculata, including BAA and three other andrographolide dimers. The study also showed that BAA and two stereoisomers stimulated phagocytosis and inhibited growth in M1 cells suggesting that these compounds can induce differentiation of these cells. Although several other compounds found in the Andrographis extract had similar effects on M1 cells, BAA and its stereoisomers had the strongest effects. Many experiments on Andrographis have focused on its anti-inflammatory and immunostimulant properties; however, there have been a small number of studies on the cardiovascular effects of Andrographis. These studies were prompted by the use of Andrographis extract for hypertension in Malaysian traditional medicine. The authors found that administration of Andrographis extract transiently reduced the blood pressure of rats. Furthermore, they found that administration of pure andrographolide did not produce a similar reduction in blood pressure suggesting that other compounds were eliciting the effect (27,28). Further studies by this group investigated how 14-deoxyandrographolide and 14-dideoxy-11,12-didehydroandrographolide, two compounds also found in Andrographis extract, might be responsible for the observation (29-31). Based on our results and several other studies on the role of TRPV4 in the vascular system, it is possible that activation of TRPV4 by BAA also might contribute to the observed reduction in blood pressure. A recent paper suggests that TRPV4 activation may result in smooth muscle hyperpolarization and arterial dilation (32). The

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a

The abbreviations used are: TRP, transient receptor potential; TRPV, vanilloid TRP; TRPM, melastatin TRP; TRPA, ankyrin TRP; 4αPDD, 4-α-phorbol-12,13-didecanoate; HEK293T, human embryonic kidney 293T; DMEM, Dulbecco's modified Eagle's Medium; EET, epoxyeicosatrienoic acid, NF-κB, nuclear factor κB; HPLC, high performance liquid chromatography; NMR, nuclear magnetic resonance; CsMES, cesium methanesulfonate; BAPTA, 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; 2-APB, 2-aminoethoxydiphenyl borate; SDS, sodium dodecyl sulfate; PVDF, polyvinylidine difluoride; GFP, green fluorescent protein; TCM, traditional Chinese medicine; BAA, bisandrographolide A; dqfCOSY, double quantum filtered correlation spectroscopy; HMQC, heteronuclear muliple quantum coherence; HMBC, heteronuclear multiple bond correlation; NOESY, nuclear Overhauser effect spectroscopy; SEM, standard error of the mean. FIGURE LEGENDS

Figure 1. A compound from an extract of the plant Andrographis paniculata activates TRPV4 channels overexpressed in HEK293T cells. A, Current-voltage relationships measured in response to voltage ramps from –80 mV to +80 mV under control conditions (black trace) or after addition of 10 µg/mL of Fraction H11 to the bath (red trace). B, Time course of TRPV4 activation by Fraction H11 for the experiment shown in A. Symbols represent current at –80 mV (open circles) and +80 mV (filled triangles) measured during consecutive voltage ramps applied at 3.5 sec intervals. The solid red bar shows presence of Fraction H11 in the bath. C, Current-voltage relationships measured before (black trace) and after (red trace) addition of 2.6 µM purified BAA to the bath. BAA was purified from Fraction H11 by HPLC. D, Time course of TRPV4 activation by BAA. Symbols are same as in B. E, Structure of BAA. F, Dose-response curve for activation of TRPV4 current (EC50 = 950 nM). Number of trials at each dose is shown in parentheses. Error bars ± SEM. Figure 2. BAA does not activate closely related TRPV channels. Experiments showing application of 2.6 µM BAA to HEK293T cells transiently overexpressing: A, TRPV1; B, TRPV2 or C, TRPV3 cDNA.

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*This work was funded by the Howard Hughes Medical Institute (DEC) and the National Institutes of Health [CA24487](JC). P. Smith was supported by a training grant from the National Institutes of Health. K. Maloney was supported by an NSF Graduate Research Fellowship. Production of the plant extract library was funded by grants from the Starr Foundation and the National Institutes of Health [R21AT001979] to the Osher Institute at Harvard Medical School. S.H. Yuspa From the National Cancer Institute kindly provided 308 cells. Constructs for rat TRPV1 and rat TRPV2 were kindly provided by D. Julius. We thank D. Eisenberg and T. Kaptchuk of the Osher Institute for information about the plants used to make the library of extracts.

Current-voltage relationships (left panels) are in response to voltage ramps from –80 mV to +80 mV. Traces recorded under control bath conditions are shown in black; traces recorded after addition of 2.6 µM BAA to the bath are shown in red, and traces recorded after addition of a known agonist for the channel (A, 100 nM capsaicin; B, 500 µM 2-APB and C, 100 µM 2-APB) are shown in blue. Time course of the response in each experiment is shown in the right panels. Symbols are the same as in Figure 1B. The red bars indicate the presence of BAA in the bath solution; the blue bars indicate the presence of the known agonist for each type of channel.

Figure 4. BAA activates TRPV4 current in cell-free patches. A, Current-voltage relationship elicited by voltage ramps from –80 mV to +80 mV in outside-out patches excised from HEK293T cells overexpressing TRPV4 cDNA. Ramps were applied at 3 second intervals. Black trace recorded under control conditions; red trace recorded in presence of 5 µM BAA. B, Time course of response to BAA. Red bar indicates presence of BAA in the bath. Symbols are the same as in Figure 1B. Figure 5. Andrographolide does not activate TRPV4 channels. A, Structure of andrographolide. B, Current-voltage relationship recorded in response to voltage ramps from –80 mV to +80 mV in control bath solution (black trace), after addition of 500 µM andrographolide to bath (green trace) and in the presence of both 500 µM andrographolide and 10 µM 4αPDD (blue trace). C, Time course of TRPV4 activation for experiment shown in B. Symbols are the same as in Figure 1B.

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Figure 3. BAA activates endogenous TRPV4 channels. A, Whole-cell voltage clamp recording of a mouse 308 cell (from a keratinocyte cell line) after perfusion of 10 µM 4αPDD. Black trace recorded in response to a voltage ramp from –80 mV to 80 mV under control conditions; blue trace recorded after addition of 10 µM 4αPDD to the bath. Activation of a current in response to 4αPDD occurred in 7 of 16 cells. B, upper panel, Whole-cell voltage clamp recording of a mouse 308 cell after addition of 2.6 µM BAA to the bath. Black trace recorded under control conditions; red trace recorded after addition of BAA. Activation of a current in response to BAA occurred in 6 of 11 cells. Lower panel, Time course of activation by BAA. Symbols are same as in Figure 1B; the red bar indicates presence of 2.6 µM BAA in the bath.






Supplementary Table I. 1H and 13C NMR chemical shifts (į) of BAA 1

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 *

H

1.21 (m), 1.48 (dt, J = 13.4, 3.2) 1.13 (m), 1.74 (m) 3.3* (m) 1.2* 1.31 (m), 1.82 (m) 1.95 (m), 2.39 (m) 1.76 (m) 1.61 (m), 1.87 (m) 2.95 (m) 7.57 (dt, J = 0.9, 1.8) 4.87 4.49, 4.85 1.17 (s) 3.40 (d, J = 11.2), 4.06 (d, J = 11.2) 0.65 (s)

13

1

C 39.4

1’

1.2 , 1.5

37.8 80.6 43.2 56.6 25.1

2’ 3’ 4’ 5’ 6’

39.0 148.6 55.9 40.3 67.7 39.2 134.6 150.6 72.2 175.5 108.0 23.2 64.6

7’ 8’ 9’ 10’ 11’ 12’ 13’ 14’ 15’ 16’ 17’ 18’ 19’

15.0

20’

1.1*, 1.75 (m) 3.4* 1.28 (m) 1.42 (dd, J = 13.0, 4.3), 1.83 (m) 2.09 (m), 2.46 (m) 2.41 (m) 6.89 (dd, J = 10.3, 15.7) 6.16 (d, J = 15.7) 7.39 (d, J = 2.0) 5.24 (dd, J = 2.0, 4.3) 4.5*, 4.8* 1.23 (s) 3.36 (d, J = 11.0), 4.13 (d, J = 11.0) 0.86 (s)

Obscured by overlap with other signals.

16

*

*

H

13

C 39*

38* 80.8 43.2 55.5 24.0 37.5 149.8 62.5 40.5 137.4 122.2 130.5 148.2 83.0 173.5 109.0 23.1 64.8 16.0