Sequence information of a variety of peptides that were described in the literature to be able to penetrate throught cellular membranes were collected and the ...
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Cell penetrating peptides: a comparative transport analysis for 474 sequence motifs Katrin Ramaker, Maik Henkel, Thorsten Krause, Niels Röckendorf, Andreas Frey Drug Delivery, January 2018, Taylor & Francis DOI: 10.1080/10717544.2018.1458921
Cell Penetrating Peptides: A comparative study on uptake e ciency What is it about? Sequence information of a variety of peptides that were described in the literature to be able to penetrate throught cellular membranes were collected and the respective peptides were modi ed with a N-terminal carboxy uorescein (FAM) tag that is traceable in living cells. An assay system for comparing the abilities of the crude peptide conjugates to carry FAM as a model cargo into con uent HeLa cells was elaborated and all candidate sequences were evaluated in this system by quanti cation of cellular FAM uorescence intensities. Drastic di erences in the ability of the peptides to carry the uorophore into the cells were observed and the peptides were ranked accordingly. A strong correlation between sequence length and uptake characteristics was observed.
Why is it important? In many studies the cell penetrating peptides (CPPs) under investigation are not compared to a large number of other CPPs, and di erent cell types and culture conditions were implemented in the studies leading to a poor comparability of di erent peptides. Standardization of methodologies at di erent sites in most cases is a di cult task, we therefore wanted to device a methodology to render the comparison of di erent CPPs in a de ned system possible.
Perspectives Niels Röckendorf (Author) Research Center Borstel Datasets included in the study represent stucture activity relationships which might be useful as training sets in developing software tools for the prediction of cell penetrating peptides. Multiple peptide test systems described might be expandet to other kinds of compound libraries or di erent cell lines.
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