Laboratorio Central de Líquido Cefalorraquídeo (LABCEL). Facultad de Ciencias Médicas “Miguel Enríquez”, Universidad de. Ciencias Médicas de La Habana.
MASP-3 aggregation and its blood to cerebrospinal fluid diffusion. Alexander Ariel Padrón-González, Cristóbal González-Losada, Jairo Lumpuy-Castillo, José Alejandro Rodríguez-Pérez, Alejandro Ramos-Robledo, William Castillo-González, Alberto Juan Dorta-Contreras. Laboratorio Central de Líquido Cefalorraquídeo (LABCEL). Facultad de Ciencias Médicas “Miguel Enríquez”, Universidad de Ciencias Médicas de La Habana. Figure 1 Purpose Define MASP-3 aggregation cerebrospinal fluid diffusion.
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Methods MASP-3 and albumin from serum and cerebrospinal fluid were quantified by immune-enzimatic test in a 60 control group without inflammatory process. Q (cerebrospinal fluid/serum) ratio was performed. QMASP-3 was order and its relative frequency was calculated.
Results The different inflexion points of the relative frequency represent five different molecular orders and the aggregation forms (Figure 1). This results are associated with the molecular flow of this structures throughout blood-cerebrospinal barrier (Table 1).
Conclusion The structure that diffuses more rapidly should be the 105 KDa native structure and it is possible to associate this aggregation with native multiple structures and its molecular flow.
Table 1 Aggregation forms from its blood-cerebrospinal fluid diffusion. Aggregation order Fractions Mean molecular size (KDa) * Qalbumin (10-3) Native structure 1-7 105 2-5 2 8-28 150-200 5-9 3 29-42 300-400 10-14 4 43-57 450-600 15-19 5 57-60 600-800 20-37 *taking into account the reported molecular complexes found in serum (J Immunol Methods. 2010 Sep 30;361 (1-2):37-50. doi: 10.1016/j.jim.2010.07.006)
