MICRORNA-200B AS PREDICTIVE BIOMARKER OF CHRONIC. KIDNEY ... analysis of miR-200b which has been previously described as a mediator of renal.
Nephrology Dialysis Transplantation 30 (Supplement 3): iii472–iii489, 2015 doi:10.1093/ndt/gfv191.19
CHRONIC KIDNEY DISEASE. PATHOPHYSIOLOGY, PROGRESSION & RISK FACTORS - 2 SP299
MICRORNA-200B AS PREDICTIVE BIOMARKER OF CHRONIC KIDNEY DISEASE (CKD) IN PATIENTS UNDERGOING RADICAL NEPHRECTOMY
Francesco Trevisani1,2, Mchele Ghidini1, Paolo Manunta2, Jens Hahne1, Andrea Lampis1, Chiara Braconi1, Maria Teresa Sciarrone Alibrandi2, Laura Zagato2, Lorena Cittero2, Giacomo Dell'antonio3, Roberto Bertini4, Andrea Salonia4, Fabio Benigni4, Giovambattista Capasso5, Patrizio Rigatti6, Andrea Cestari6, Massimo Rugge7, Matteo Fassan7 and Nicola Valeri1 1 ICR, Molecular Pathology, London, United Kingdom, 2San Raffaele Institute, Nephrology, Milan, Italy, 3San Raffaele Institute, Pathology, Milan, Italy, 4San Raffaele Institute, Urology, Milan, Italy, 5Second University of Naples, Nephrology, Naples, Italy, 6Istituto Auxologico Italiano, Urology, Milan, Italy, 7University of Padua, Surgical Pathology, Padua, Italy
(>3cm) to the RCC was used for the analysis. Renal cortex (10 glomeruli) and medulla were marked and isolated by manual microdissection. miR extraction was performed using Ambion (LifeTechnologies) Recover All Isolation Kit. MiR expression analysis was performed by Nanostring (nCounter Technology) and by Real-Time PCR (LifeTechnologies). Results: Pts in the discovery cohort were stratified according to renal function calculated at 12 months from surgery: CKD pts (if eGFR ≤ 60 ml/min) and Normal Kidney Function (NKF) pts (if eGFR > 60 ml/min). No significant differences in miR-200b expression among cortex in CKD, NKF and donors were observed ( p=0.26). On the contrary miR-200b was significantly overexpressed in medulla of CKD vs. NKF patients (Figure 1; p
