Combined glucosamine and chondroitin sulfate ...

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Sep 13, 2008 - Francisco Saraiva Silva Jr. &. Natalino Hajime ... F. S. Silva Jr. e-mail: .... and heparan sulfate (Sigma, St. Louis, MO, USA) were subjected to the ...

Clin Rheumatol (2009) 28:109–117 DOI 10.1007/s10067-008-0988-8

ORIGINAL ARTICLE

Combined glucosamine and chondroitin sulfate provides functional and structural benefit in the anterior cruciate ligament transection model Francisco Saraiva Silva Jr. & Natalino Hajime Yoshinari & Rondinelle Ribeiro Castro & Virgínia Cláudia Carneiro Girão & Margarida Maria Lima Pompeu & Judith Pessoa de Andrade Feitosa & Francisco Airton Castro Rocha

Received: 3 June 2008 / Revised: 3 July 2008 / Accepted: 21 July 2008 / Published online: 13 September 2008 # Clinical Rheumatology 2008

Abstract Evidence that combined glucosamine sulfate and chondroitin sulfate (Gluchon) or isolated glucosamine (Glu) modifies joint damage in osteoarthritis (OA) is still lacking. We studied joint pain and cartilage damage using the anterior cruciate ligament transection (ACLT) model. Wistar rats were subjected to ACLT of the right knee (OA) or sham operation. Groups received either Glu (500 mg/kg), Gluchon (500 mg/kg glucosamine +400 mg/kg chondroitin) or vehicle (non-treated—NT) per os starting 7 days prior to ACLT until sacrifice at 70 days. Joint pain was evaluated daily using the rat-knee joint articular incapacitation test. Structural joint damage was assessed using histology and biochemistry as the chondroitin sulfate F. S. Silva Jr. : N. H. Yoshinari Department of Rheumatology, Faculty of Medicine, University of São Paulo, São Paulo, Brazil F. S. Silva Jr. e-mail: [email protected] N. H. Yoshinari e-mail: [email protected] R. R. Castro Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Ceará, Brazil e-mail: [email protected] V. C. C. Girão : F. A. C. Rocha Department of Internal Medicine, Faculty of Medicine, Federal University of Ceará, Ceará, Brazil

(CS) content of cartilage by densitometry (microgram per milligram dried cartilage), comparing to standard CS. The molar weight (Mw) of the CS samples, used as a qualitative biochemical parameter, was obtained by comparing their relative mobility on a polyacrylamide gel electrophoresis to standard CS. Gluchon, but not Glu, significantly reduced joint pain (P

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