comunidades Maya-Chontales del municipio de Nacajuca, Tabasco. Tesis Doctoral,. Universidad Juarez Autonoma de Tabasco. Mahboubi M, Haghi G, ...
COMPARATIVE PHYTOCHEMICAL EVALUATION AND BIOLOGICAL ACTIVITY SCREENING OF Murdannia nudiflora and Tradescantia pallida
Submitted By Student ID:11146004 Batch: Spring 2011
Department of Pharmacy BRAC University March, 2015
Comparative Phytochemical Evaluation and Biological Activity Screening of Murdannia nudiflora and Tradescantia pallida
The dissertation is submitted to the Department of Pharmacy of BRAC University in partial fulfillment of the requirement for the Degree of Bachelor in Pharmacy.
Submitted By Student ID: 11146004 Batch: Spring 2011
Department of Pharmacy BRAC University March, 2015
Abstract: The study was aimed to evaluate the phytochemicals and pharmacological investigation on commelinaceae family which have high rate of growth and found as weeds of notably important therapeutic values. The plants were chosen from different genus, one indigenous, Murdannia nudiflora from the genera Murdannia and the other exotic, native to Mexico and south American countries Tradescantia pallida from the genera Tradescantia which are mainly used as a ornamental plant to find out the presence of notable compounds present and to justify the claims provided in traditional medicine in a more precise and accurate approach of science. Preliminary phytochemical screening shows the presence of carbohydrate, alkaloids, tannins , saponins and flavonoids. Cytotoxic analysis with in vitro brine shrimp lethality assay showed LC 50 at a higher concentration of 158.638 µg/ml for Murdannia nudiflora and 833.85 µg/ml for Tradescantia pallida, quite low in comparison to the activity of vincristine sulphate. For toxicokinetic study, the extract shows degree of moderate lethality of more than 60% for Murdannia nudiflora and more than 50% for Tradescantia pallida at a concentration of 640µg/ml.. The percentage of DPPH radical scavenging for antioxidant studies in Murdannia nudiflora ranges from 96.825% to 82.539%, while that of Tradescantia pallida ranges from 95.238% to 84.127% and that of ascorbic acid ranges from 96.825% to 87.302%. The IC 50 values for ascorbic acid was 4.209x1013 µg/ml, for Murdannia nudiflora was 1.51x10-10µg/ml and for Tradescantia pallida was 5.48x10-10µg/ml. Analgesic activity was evaluated using in vivo acetic acid induced writhing method were 0.7% acetic acid used for the induction of pain. This study is used to evaluate both central and peripherial pain and the potential is evaluated in terms of inhibition of writhing. The mean inhibition of writhing by the samples (Murdannia nudiflora and Tradescantia pallida) were found out to be of statistically significant (p
