Complete Chiral Solutions

SIMPLIFIED CHIRAL SEPARATIONS

Complete Chiral Solutions HPLC

•

SFC

•

Process

Amylose-2: Unique chlorinated phase widens the range of enantioselectivity Cellulose-2: Novel chlorinated phase improves your chiral screen success rate Cellulose-1: Very cost-effective alternative to *Chiralcel® OD-H®

Simplify chiral separations, from analytical to process scale. All at sensible cost! Lux™ chiral columns and media simplify the separation process in three ways: • Unique and traditional phases that increase the success rate of your chiral screen • Complete compatibility in Normal Phase, Polar Organic, SFC, and Reversed Phase methods – no switching columns! • Availability in 3 µm and 5 µm packed columns as well as 20 µm bulk media for process scale purification Chirality can be complex, but its separation shouldn’t be.

If you are not completely satisfied with Lux™ analytical columns, send in your comparative data to a similar product within 45 days and KEEP THE COLUMN FOR FREE. 2


Choosing the Right Chiral Stationary Phase Features

Lux™ Cellulose-2

Lux™ Cellulose-1

Lux™ Amylose-2

Broad chiral recognition For screening of new chiral compounds Cellulose backbone Amylose backbone High loading capacity Scalable 3 μm and 5 μm media Process media- 20 µm

†

Low MS bleed Pressure stable up to 300 bar pH stable 2-9 Stable in NP, Polar Organic, SFC and RP conditions Novel selectivity Cost-effective, guaranteed alternative to *CHIRALCEL® OD-H® Satisfaction guaranteed

Table of Contents High Efficiency for Improved Separations Analytical...................................................................4 Preparative and Process...........................................5 Applications Lux™ Amylose-2........................................................6 Lux™ Cellulose-2.....................................................14 Lux™ Cellulose-1.....................................................20 SFC.........................................................................24 Preparative and Process.........................................25 Ordering Information............................................................26

Trademarks

Lux, Axia, SecurityGuard and Phenex are trademarks of Phenomenex, Inc. *CHIRALCEL, CHIRALPAK, OD-H, and AD are registered trademarks of DAICEL Chemical Industries, Ltd.

Disclaimer

Please inquire for 20 µm Lux Amylose-2 media. Comparative separations may not be representative of all applications. Subject to Phenomenex Standard Terms & Condi-

†

tions, which may be viewed at www.phenomenex.com/TermsAndConditions. © 2009 Phenomenex, Inc. All rights reserved.

Phenomenex | Web: www.phenomenex.com

3

3 µm High Efficiency for Improved Resolution Lux™ 3 µm phases offer unmatched efficiencies for improved resolution of difficult separations. 18218

Nisoldipine 2

H3C

200

App ID 18218

Lux™ Cellulose-1 Rs = 1.78

300

CH3

1

3 µm

400

H N

H3C

mAU

100

O CH3

O O

O NO2

0 18219 0

2

4

6

8

10

12

14 min

mAU 400


200

1

App ID 18219

5 µm

300

2

100

Conditions for both columns: Dimensions: 250 x 4.6 mm Mobile Phase: 0.1 % Diethylamine in Hexane / 0.1 % Diethylamine in Isopropanol (90:10) Flow Rate: 1 mL/min Detection: UV @ 220 nm Temperature: Ambient

0

0

2

4

6

8

10

12

14 min

17469

Mephenesin

CH 3

mAU

3 µm


400

200

O 2

1

17470 0 0

2

4

6

8

10


200

1

2

App ID 17470

5 µm

400

OH

12 min

mAU

600

OH

App ID 17469

600


0 0 17471

2

4

6

8

10

12 min

Mianserin

mAU 600

3 µm


200

1

2

N

App ID 17471

400

0

N

17477 0

2

4

6 min

mAU 600

1

5 µm

200

App ID 17477


400

2

0

0

4

2


4

6 min

Conditions for both columns: Dimensions: 250 x 4.6 mm Mobile Phase: 0.1 % Diethylamine in Acetonitrile / 0.1 % Diethylamine in Isopropanol (95:5) Flow Rate: 1 mL/min Detection: UV @ 220 nm Temperature: Ambient

CH3

Higher Purity Preparative Separations


With award-winning Axia™ technology, analytical-like efficiency is achieved in a preparative column format. 18283

High Efficiency trans-Stilbene Oxide from Analytical to Preparative 4.6 mm ID N = 70,664 a = 1.19

App ID 18283

1

2

18284 0

Column: Dimensions: Mobile Phase: Detection: Temperature:

Lux™ 5 µm Cellulose-2 250 x 4.6 mm Hexane / Isopropanol (90:10) UV @ 220 nm Ambient

18 min

21.2 mm ID N = 77,180 a = 1.22

App ID 18284

1

2

18285 0


Lux™ 5 µm Cellulose-2 250 x 21.2 mm Hexane / Isopropanol (90:10) UV @ 220 nm Ambient


Lux™ 5 µm Cellulose-2 250 x 50 mm Hexane / Isopropanol (90:10) UV @ 220 nm Ambient

Dimensions: Mobile Phase: Flow Rate: Detection: Temperature: Injection:

100 x 4.6 mm Methanol / Isopropanol (90:10) 1 mL/min UV @ 220 nm Ambient 5 mg in 2 µL





2006 R&D 100 Award Recipient We invite you to view the award winning high efficiency packing process at: www.phenomenex.com/info/Axia

18 min

50 mm ID N = 75,156 a = 1.19

App ID 18285

1

2

0

18 min

17780

Direct Scale Up of Methylcarbamol on Lux™ Cellulose-1 1 2

App ID 17780

5 µg a = 1.93 17781 0

1

2 1

min

Increase ID

32 mg a = 1.80

App ID 17781

2

17782 0

1

2

11

Increase Length

80 mg a = 1.84

0

1

min

2

3

4

App ID 17782

22

5

6

7

min


5

Amylose-2 Phenyl rings contribute to π-π interactions.

Cl

Lux™ Amylose-2 uses the unique chiral selector amylose tris(5-chloro-2-methylphenylcarbamate)

O NH group provides a balance of intramolecular hydrogen bonding with solute hydrogen bonding.

H

H C

Cl

O C

H

C H

H C

H

O

C

C

N

N

O

H

H H

C

H

C

C

H

H

C

O

H

O

H

O

Cl

N C O

H

C

H H

H

Amylose forms helical chains, providing unique steric interactions through various grooves and cavities. Chlorine atoms have strong electronegativity, contributing to dipole interactions.

Unique Selectivity = More Possibilities 6


This unique chlorinated phase widens the range of enantioselectivity Lux Amylose-2 ™

The chiral selector is an amylose tris(5-chloro-2-methylphenylcarbamate) The selectivity of Lux™ Amylose-2 is an excellent complement to your chiral screen. Using a unique derivitization, it offers a broad range of enantioselectivity, previously unavailable commercially. Studies show that for a set of 14 racemates of varying properties, the 5-chloro-2-methylphenylcarbamate derivative of amylose was the only phase to separate all 14 compounds with acceptable selectivity, compared to 11 other chiral amylose phases.1 Lux™ Amylose-2 provides a balanced ratio of free NH groups to intermolecular hydrogen bonded NH groups, which contributes to the high chiral recognition abilities of this phase.1 Research has also supported that ortho substitutions of methyl or chloro groups on amylose phenylcarbamate derivatives have strong chiral resolving power.1 1. Bezhan Chankvetadze, Eiji Yashima, Yoshio Okamoto, J. Chromatography A, 694 (1995) 101-109


7

Lux Amylose-2 vs. *ChiralPAK AD -H ™

®

®

Lux™ Amylose-2 offers novel selectivity, improving chances of chiral resolution during multiple column screening processes. 18098

2-Phenylcyclohexanone mAU

1

Lux™ 5 µm Amylose-2 a = 1.24

App ID 18098

2

100

0

O

18097 0

2

4

6

8

10

12

min

mAU

*CHIRALPAK® 5 µm AD®-H a = 1.04

App ID 18097

100

1 2


0 0

2

4

6

8

10

12

min

18116

Acebutolol

O

CH3 OH

mAU

Lux 5 µm Amylose-2 a = 1.41 ™

mAU

2

18115

0

2

*CHIRALPAK® 5 µm AD®-H

1000

4

CH3

O

200

0

H N

O

1

App ID 18116

400

6

8

CH3

N H

min

1,2

App ID 18115

a = 1.00

H 3C


0 0

2

4

6

8

min

18103

Chlorpheniramine

CI

CH3 mAU


H3C

1

N

2

0

App ID 18103

200

18104

0

2

4

6

8

N

min

mAU


400

1

2

App ID 18104

a = 1.24

200

0 0

8

2

4

6

8


min

Phenomenex | Web: www.phenomenex.com *CHIRALPAK and AD are registered trademarks of DAICEL Chemical Industries, Ltd. Comparative separations may not be representative of all applications.


18106

Dimethindene maleate 1

mAU


CH3

2

N CH3

App ID 18106

200

100

H3C

0 18105 0

2

4

6

8


mAU

1

App ID 18105

a = 1.08 100

N

min

2


0

0 18108

2

4

6

8

min

Mephenesin mAU


CH 3

1

App ID 18108

200 2

18107

O

OH OH

0 0

2

4

6

8

min

mAU


a = 1.09

1

2

App ID 18107

200


0 0

2

4

6

8

min

18112

Pheniramine mAU


2

App ID 18112

1

200

N

N

CH3

0 18111 0

CH3 2

4

6

8

min

mAU


800

1

2

App ID 18111

a = 1.05 400

0 0

2

4

6

8

min


Additional applications are available upon request.

Phenomenex | Web: www.phenomenex.com *CHIRALPAK and AD are registered trademarks of DAICEL Chemical Industries, Ltd. Comparative separations may not be representative of all applications.

9

Lux Amylose-2 Increases Chiral Success ™

Lux™ Amylose-2 offers complementary selectivity to Lux™ Cellulose-1 and Lux™ Cellulose-2, increasing the chances of successful chiral resolution. 18121

F3C

Halofantrine

OH

mAU


1000

CH3

N

1 2

App ID 18121

CH3

18122

0

0

1

2

3

4

mAU

5

CI

CI

min

1,2

Lux™ 5 µm Cellulose-2 a = 1.00 App ID 18122

1000


0 0

1

2

3

4

5

min

18118

Bisoprolol

mAU


CH 3 H 3C

200

1

App ID 18118

400

CH 3

O

O

CH 3

2

18127

N H

O OH

0

mAU

0

10

min

Lux™ 5 µm Cellulose-2 a = 1.00

1,2

App ID 18127

400

200


0 0

10

min

18120

Disopyramide

H3C

N

mAU

N


NH2

2

CH3 O

0 18119

0

2

4

6

8

10

12

14

min

mAU 1,2

100

App ID 18119


0

0

2

4

6

CH3

1

App ID 18120

100

10

CH3

8


10

12

14

min



18124

Metomidate mAU


1

App ID 18124

100

2

0

18123 0

mAU

2

4

6

1


10

12

14

2

4

6

H3O N

2

0

0

N

min

App ID 18123

100

8

H3C O

8

10

12

14


min

18126

Nimodipine

H3C

H N

CH3


1 2

App ID 18126

mAU

O

O

H3C

O

CH3

CH3

O

0

NO2

18125

0

10

20

mAU

min

1,2

Lux 5 µm Cellulose-2 a = 1.00 ™

400

App ID 18125

200

0 0

10


min

18112

Pheniramine Lux™ 5 µm Amylose-2 a = 1.27

N

1 2

200

App ID 18112

mAU

N

CH3

CH3

0 18128 0

2

4

6

8

min

mAU 800


App ID 18128

1,2

400


0 0

2

4

6

10

min


11

Lux Amylose-2 Increases Chiral Success (continued) ™

Lux™ Amylose-2 offers complementary selectivity to Lux™ Cellulose-1 and Lux™ Cellulose-2, increasing the chances of successful chiral resolution. 18130

Acebutolol

O

CH3 OH

mAU 1000

Lux 5 µm Amylose-2 a = 1.41 ™

800

H N

O

CH3

O

1

CH3

App ID 18130

600 400 2

200 18129

0

0

2

4

6

8

10

12

14

H3C

N H

min

mAU 1000


800

1,2

App ID 18129

600 400 200

Conditions for both columns: Dimensions: 250 x 4.6 mm Mobile Phase: 0.1 % Diethylamine in Acetontrile / 0.1 % Diethylamine in Isopropanol (95:5) Flow Rate: 1 mL/min Detection: UV @ 220 nm Temperature: Ambient

0 0

2

4

6

8

10

12

14

min

18132

Betaxolol Lux™ 5 µm Amylose-2 a = 1.28

200

1

2

100

0

18131

0

2

O

CH3

App ID 18132

mAU

4

6

8

H3C

N H

O OH

min

mAU


1,2

App ID 18131

200

100


0 0

2

4

6

8

min

O

18136

O

Indapamide HN

mAU

Lux™ 5 µm Amylose-2 1 a = 1.39

CH3

2

400

18135

0

2

4

mAU

6

8

min

1,2

App ID 18135


800

400

0 0

12

2

4

NH 2

N

App ID 18136

800

0

O S

6


8

min


Cl


18138

Ornidazole mAU

OH CI

2

App ID 18138

Lux™ 5 µm Amylose-2 1 a = 2.42

200

100

N

O2N

0

CH3 N

18137 0

mAU

2

4

6

8

min

Lux™ 5 µm Cellulose-2 1,2 a = 1.00 App ID 18137

400

200

0 0

2

4

6

8

Conditions for both columns: Dimensions: 250 x 4.6 mm Mobile Phase: 0.1 % Diethylamine in Methanol / 0.1 % Diethylamine in Isopropanol (90:10) Flow Rate: 1 mL/min Detection: UV @ 220 nm Temperature: Ambient

min

18140

Temazepam Lux™ 5 µm Amylose-2 a = 1.37

200

App ID 18140

mAU

1

2

100

O

H3C N

O H N

0

18139

0

Cl 2

4

6

8

min

mAU


1

2

App ID 18139

200

100


0

0

2

4

6

8

min

18144

Tetramisole mAU


H

2 200

0

N

S

1

App ID 18144

400

N

18143 0

2

4

6

8

min

mAU 1

2

App ID 18143


300

200

100

0

0

2

4

6

8

min


Additional applications are available upon request.


13

Cellulose-2 Phenyl rings contribute to π- π interactions.

Cl

Lux™ Cellulose-2 uses the novel chiral selector cellulose tris(3-chloro-4-methylphenylcarbamate)

H

O Electronegative atoms, such as oxygen, nitrogen, and chlorine are responsible for hydrogen bonding.

C

Cl

H H

C H

O C

N

O

O

C

C

N

C

H

H H

H

H

H

H

C

H C

H

C

H

O

Cellulose backbones form linear chains, creating various grooves and cavities which provide steric and inclusion interactions.

C

O O

H N

C H

O

C H

H

Cl Chlorine atoms have strong electronegativity, contributing to dipole interactions.

Novel Selectivity = More Possibilities 14


This universal, novel chlorinated cellulose phase improves your chiral screen success rate Lux Cellulose-2 ™

The chiral selector is a cellulose tris(3-chloro-4-methylphenylcarbamate) The unique phase of Lux™ Cellulose-2 makes it an ideal choice for all chiral screening protocols. It provides excellent complementary selectivity to Lux™ Cellulose-1 and Lux™ Amylose-2. The introduction of either electron-donating or electronwithdrawing groups, such as chlorine, tends to improve the optical resolution abilities of chiral stationary phases (CSPs) with phenylcarbamate derivatives. In addition, substitution at the meta or para position on the phenyl moiety is considered more beneficial than at the ortho position to establish higher chiral recognition.2 Studies show that for a set of 14 racemates of varying properties, the 3-chloro-4-methylphenylcarbamate derivative of cellulose was one of two derivatives to separate all 14 compounds with reasonable selectivity.2 2. Bezhan Chankvetadze, Eiji Yashima, Yoshio Okamoto, J. Chromatography, 670 (1994) 39-49


15

Lux Cellulose-2 vs. *Chiralcel OD-H ™

®

®

Lux™ Cellulose-2 offers novel selectivity, improving chances of chiral resolution during multiple column screening processes. 17309

Bupivacaine mAU


H 3C

200

2

H 3C

N

App ID 17309

1

O

N H CH 3

0 0

17310

2

4

6

8

10

min

mAU

*CHIRALCEL® 5 µm OD-H® a = 1.12

2

App ID 17310

200

1


0 0

2

4

6

8

10

min

17323

Tolperisone mAU


600

O 1

400

App ID 17323

2

200

N CH 3

H 3C

17322 0 0 mAU

2

4


600

6

min

2

1

App ID 17322

400

200


0 0

17367

2

4

6

min

Miconazole

mAU


800

N 1 2

400

200

0 17368

Cl App ID 17367

600

0

2

4

mAU

1


800

6

App ID 17368

200 0

16

4

O Cl

2

400

2

Cl

min

600

0

N

6


min

Phenomenex | Web: www.phenomenex.com *CHIRALCEL and OD-H are registered trademarks of DAICEL Chemical Industries, Ltd. Comparative separations may not be representative of all applications.

Cl


Tramadol

17330 mAU


H 3C

1

N

2

App ID 17330

400

200

H H 3 CO OH

0 17331 0

4

2

6

*CHIRALCEL® 5 µm OD-H®

mAU

1

min

2

a = 1.13 App ID 17331

400

CH 3

200


0 0

17349

2

4

6

min

Mephenytoin

mAU


400

1

H N

App ID 17349

2

200

H 3C

N O

17350 0 0

2

4

6

8

O

C H3

min

mAU


a = 1.19

1 2

App ID 17350

400

200


0 0

17388

2

4

6

8

min

N-(3,5-Dinitrobenzoyl)-a’-methylbenzylamine

mAU


1400 1200 1000

O N+

2

600 400 200 0 0 17387

5

O

–

App ID 17388

800

1

10

15

20

min

H N

O

–

N+ O

O


1200

a = 1.69

1000 800

App ID 17387

mAU 1400

1

600 2

400 200


0 0

5

10

15

20

min


17


®

®

(continued)

Lux™ Cellulose-2 offers novel selectivity, improving chances of chiral resolution during multiple column screening processes. 17395

Econazole

mAU


1000 800

1

N

2

Cl App ID 17395

600 400 200

N Cl

17396 0 0

2

4

6

mAU

1

1000

App ID 17396

600 400 200 0 0

17420

2

Cl

2


800

O

min

4

6


min

Terfenadine

C(CH 3 ) 3

mAU

Lux 5 µm Cellulose-2 a = 1.28 ™

200

N App ID 17420

1 2

OH OH

0 17421 0

2

4

6

min

mAU

200

1

App ID 17421

*CHIRALCEL® 5 µm OD-H® a = 1.39 2


0 0

17461

2

4

min

Etozolin

mAU

1

2000

0 17462

6


0

S

N

O

App ID 17461

2

2

4

6

8

N O

O CH 3

min

mAU


2000

1

App ID 17462

2


0 0

18

2

4

6

8

min


CH 3


17455

Nomifensine

mAU


NH 2 1

N

App ID 17455

2

200

CH 3

0 0

2

4

min

17456 mAU


a = 1.11

1

App ID 17456

2

200


0 0

17458

2

4

min

Mirtazapine

mAU


1 2

App ID 17458

200

N N

N

0 17459

0

mAU

2

4

6 1


min

2

App ID 17459

a = 1.24 200


0 0

17464

2

4

6

min

Bifonazole

mAU

N

600


N

1 2

200

App ID 17464

400

0 0

2

4

6

8

10

min

17465 mAU


600

1 2

a = 1.07

App ID 17465

400

200


0 0

2

4

6

8

10

min


19

Cellulose-1 Lux™ Cellulose-1 uses the chiral selector cellulose tris(3,5-dimethylphenylcarbamate)

Electronegative atoms, such as oxygen and nitrogen, are responsible for hydrogen bonding.

H

H C

H

H

C H

O

N C C H

H

C

H C

H

N

O

H

C

C H

H

O

C

C

H

H

C

O H

H

Phenyl rings contribute to π- π interactions.

H

C

O O

H

O H

Cellulose backbones form linear chains, creating various grooves and cavities which provide steric interactions.

H

H

H

H

C

N

H

C O

H

C

H

H

Guaranteed replacement for CHIRALCEL® OD-H® 20


Use for new methods or as a guaranteed* replacement for ANY method on *CHIRALCEL OD-H Lux Cellulose-1 ®

®

™

The derivative is cellulose tris(3,5-dimethylphenylcarbamate) Lux™ Cellulose-1 not only is your cost-effective alternative to CHIRALCEL® OD-H®, but it also can provide greater resolution for many applications, as shown in this brochure. This particular chiral selector has well established abilities in resolving a wide range of racemates. Lux™ Cellulose-1 provides enhanced resolution for a variety of applications compared to similar CSPs. This derivative of cellulose has a well established history of broad enantioselectivity. The substitution of the electron donating methyl groups at the 3 and 5 position enhance the activity of the phenyl group. This leads to enhanced enantioselectivity for aromatic and conjugated compounds.

*

If you are not completely satisfied with Lux™ analytical columns, send in your comparative data to a similar product within 45 days and KEEP THE COLUMN FOR FREE.

Phenomenex | Web: www.phenomenex.com *CHIRALCEL and OD-H are registered trademarks of DAICEL Chemical Industries, Ltd.

21


®

®

Lux™ Cellulose-1 offers exceptional separations for your existing polysaccharide methods. 17314

Naringenin

mAU


1000 800

1

OH 2

400 200 0

O

HO

App ID 17314

600

17316 0

2

4

6

OH

min

O

mAU 1 1000


600

a = 1.24

2

App ID 17316

800

400 200 0 0

2

4

6

Conditions for both columns: Dimensions: 250 x 4.6 mm Mobile Phase: 0.1 % Formic acid in Hexane / 0.1 % Formic acid in Isopropanol (60:40) Flow Rate: 1 mL/min Detection: UV @ 220 nm Temperature: Ambient

min

Orphenadrine

17327

mAU 1


600

CH3 2

App ID 17327

400

200

0 17328 0

2

4

6

O

N

C H3

C H3

min

mAU 1


600

a = 1.94

2

App ID 17328

400

200


0 0

2

4

6

min

Bisoprolol

17319 mAU


1

2

200

0

O

CH 3 App ID 17319

400

CH 3 H 3C

N H

O OH

17320 0

2

4

6

8

min

mAU 1 400


a = 1.83

App ID 17320

2

200

0 0

22

2

4

6

8

CH 3

O


min



17341

Mebeverine

mAU


600

200

0 17342 0

2

4

6

8

10

CH 3

O

2

App ID 17341

400

1

12

H 3CO

N

O

CH 3 H 3CO

OCH 3

min

mAU 1

600

2


App ID 17342

a = 1.24

400

200

0 0

2

4

6

8

10

12


min

Toliprolol 17372 mAU


1

OH 2

H 3C

O

H N

CH3

App ID 17372

400

200

CH3

0 17373

0

1

2

3

4

min

mAU


400

1

a = 1.50

App ID 17373

2

200

0 0

1

2

3

4


min

Dimethindene 17376 mAU


CH3

1

2

App ID 17376

400

6

min

200

0 17377

N CH3

H3C 2

0

4

N

mAU 1 400


a = 1.20

App ID 17377

2

200

0 0

2

4

6

min

Conditions for both columns: Dimensions: 250 x 4.6 mm Mobile Phase: 0.1 % Diethylamine in Methanol / 0.1 % Diethylamine in Isopropanol (90:10) Flow Rate: 1 mL/min Detection: UV @ 220 nm Additional Temperature: Ambient

applications are available upon request.


23

Pressure Rated for SFC From analytical to Axia™ packed preparative columns, Lux™ chiral columns offer complementary selectivities, high efficiency, and pressure stability up to 300 bar (4300 psi) for your SFC separations. 18203

trans-Stilbene Oxide

mAU

Lux™ 5 µm Cellulose-1

300

App ID 18203

200 150 100 50

0

1

2

100

3

1

60 40 20 0 0

0.1

0.2

0.3

0.4

0.5

Pfizer Analytical Research Center Lux™ 5 µm Cellulose-1 Ghent University 250 x 4.6 mm 00G-4459-E0 0.1 % Diethylamine with 0.1 % Trifluoroacetic acid in Methanol / Carbon Dioxide (25:75) Flow Rate: 2 mL/min Detection: UV @ 220 nm Temperature: Ambient

Column: Dimensions: Part No.: Mobile Phase:

min

App ID 18205

80

4

2

Lux™ 3 µm Cellulose-1 OPTIMIZED FOR SPEED

0.6

0.7


Lux™ 3 µm Cellulose-1 50 x 4.6 mm 00B-4458-E0 0.1 % Diethylamine with 0.1 % Trifluoroacetic acid in Methanol / Carbon Dioxide (25:75) Flow Rate: 4 mL/min Detection: UV @ 220 nm Temperature: Ambient

min

18107

Bendroflumethiazide

mAU 1

2

App ID 18207

Lux™ 5 µm Cellulose-2 200

0 18206 0

2

4

6

8 1

mAU

min

App ID 18208

300

200

100

0 0

0.1

0.2

0.3


Lux™ 5 µm Cellulose-2 250 x 4.6 mm 00G-4457-E0 0.1 % Diethylamine with 0.1 % Trifluoroacetic acid in Methanol / Carbon Dioxide (30:70) Flow Rate: 2 mL/min Detection: UV @ 273 nm Temperature: Ambient

2


400

0.4

0.5



min

Benzoin 18209 1

mAU

2

Lux™ 5 µm Cellulose-2

200

App ID 18210

150

100

50

0 18211 0

1

2

3

4

5

6

7

8

min

Lux™ 5 µm Cellulose-2 250 x 4.6 mm 00G-4457-E0 0.1 % Diethylamine with 0.1 % Trifluoroacetic acid in Methanol / Carbon Dioxide (15:85) Flow Rate: 2 mL/min Detection: UV @ 254 nm Temperature: Ambient

2

100 80 60

App ID 18211


120

40 20 0 -20 0

24


1

mAU

0.1

0.2

0.3

0.4

0.5

0.6

0.7

”

Dr. Melissa Dunkle

2

mAU

We were very pleased with how the Lux Cellulose-1 and Lux Cellulose-2 columns performed for chiral separations using SFC

1

250

0 18205

“

0.8

0.9



min

Phenomenex | Web: www.phenomenex.com Data courtesy of Dr. Melissa Dunkle, Pfizer Analytical Research Center, Ghent University. SFC separations performed on a SandraSelerity SFC system.

Screen for Preparative Purifications


Increased resolution for more cost-effective preparative and process purifications. 18188

Bupivacaine mV

mV

220 nm ALP

10

30

0

20

-10

10

App ID 18187

20

40

-20

220 nm ALP

0

-10

-30 2

Column: Dimensions: Part No.: Mobile Phase: Flow Rate: Detection: Polarimeter: Temperature:

18189

4

6

8

10

min

0

220 nm ALP

30

60

10

40

0

20

App ID 18189

80

-10

-30

18191

6

min

mV

50

2

Flow Rate: Detection: Polarimeter: Temperature:

4

Lux™ 5 µm Cellulose-2 250 x 4.6 mm 00G-4457-E0 0.1 % Diethylamine in Hexane / 0.1 % Diethylamine in Isopropanol (90:10) 1 mL/min UV @ 220 nm PDR-CHIRAL ALP Ambient

Nomifensine

mV



18190

Econazole

0

2


Lux™ 5 µm Cellulose-2 250 x 4.6 mm 00G-4457-E0 0.1 % Diethylamine in Hexane / 0.1 % Diethylamine in Isopropanol (90:10) 1 mL/min UV @ 220 nm PDR-CHIRAL ALP Ambient

4

6

8

220 nm ALP

App ID 18190

0

mV

Tramadol

App ID 18188

18187

0

-10

min

0

2


Lux™ 5 µm Cellulose-2 250 x 4.6 mm 00G-4457-E0 0.1 % Diethylamine in Methanol / 0.1 % Diethylamine in Isopropanol (90:10) 1 mL/min UV @ 220 nm PDR-CHIRAL ALP Ambient


18191

Naringenin

mV

220 nm ALP

4

6

Indapamide 220 nm ALP

40

80

min

Lux™ 5 µm Cellulose-2 250 x 4.6 mm 00G-4457-E0 0.1 % Diethylamine in Acetonitrile / 0.1 % Diethylamine in Isopropanol (95:5) 1 mL/min UV @ 220 nm PDR-CHIRAL ALP Ambient

20 60

0 -20

20

0

-40

App ID 18193

App ID 18191

40

-60 -80 -100

-20 0

2


4

Lux™ 5 µm Cellulose-1 250 x 4.6 mm 00G-4459-E0 0.1 % Formic acid in Hexane / 0.1 % Formic acid in Isopropanol (60:40) 1 mL/min UV @ 220 nm PDR-CHIRAL ALP Ambient

6

min

0

2


4

6

min

Lux™ 5 µm Cellulose-1 250 x 4.6 mm 00G-4459-E0 0.1 % Diethylamine in Methanol / 0.1 % Diethylamine in Isopropanol (90:10) 1 mL/min UV @ 220 nm PDR-CHIRAL ALP Ambient


25

Lux Ordering Information ™

3 μm Analytical Columns (mm) 50 x 2.0

Phases Cellulose-1 Cellulose-2 Amylose-2

150 x 2.0

SecurityGuard™ Cartridges (mm) 50 x 4.6

00B-4458-B0 00F-4458-B0 00B-4456-B0 00F-4456-B0 –– 00F-4471-B0

100 x 4.6

150 x 4.6

00B-4458-E0 00D-4458-E0 00B-4456-E0 00D-4456-E0 00B-4471-E0 ––

00F-4458-E0 00F-4456-E0 00F-4471-E0

250 x 4.6

4 x 2.0*

4 x 3.0*

00G-4458-E0 00G-4456-E0 00G-4471-E0 for ID:

/10pk AJ0-8402 AJ0-8398 AJ0-8471 2.0–3.0 mm

/10pk AJ0-8403 AJ0-8366 AJ0-8470 3.2–8.0 mm

5 μm Analytical Columns (mm) 50 x 2.0


50 x 4.6

00B-4459-B0 00B-4457-B0 ––

SecurityGuard™ Cartridges (mm) 100 x 4.6

00B-4459-E0 00B-4457-E0 00B-4472-E0

00D-4459-E0 00D-4457-E0 00D-4472-E0

150 x 4.6

00F-4459-E0 00F-4457-E0 00F-4472-E0

250 x 4.6

4 x 2.0*

4 x 3.0*

00G-4459-E0 00G-4457-E0 00G-4472-E0 for ID:

/10pk AJ0-8402 AJ0-8398 AJ0-8471 2.0–3.0 mm

/10pk AJ0-8403 AJ0-8366 AJ0-8470 3.2–8.0 mm

3 µm Method Screening Kits (save 10 % when purchased as a kit)

5 µm Semi-Prep Columns (mm)

50 x 4.6 mm

250 x 4.6 mm

KH0-8483

KH0-8482

Screening Kit*

150 x 10.0

Phases Cellulose-1 Cellulose-2

*Kit contains one column of each phase.

00F-4459-N0 00F-4457-N0

250 x 10.0

00G-4459-N0 00G-4457-N0 for ID:

SecurityGuard™ Cartridges (mm) 10 x 10.0‡

/3pk AJ0-8404 AJ0-8399 9-16 mm

Column Performance Check Standard Part No.

Description

AL0-8412

Chiral Test Mix No. 5 (Lux)

Unit

Price

ea

5 μm Axia™ Packed Preparative Columns (mm) 150 x 21.2


250 x 21.2

SecurityGuard™ Cartridges (mm)

250 x 30

00F-4459-P0-AX 00G-4459-P0-AX 00F-4457-P0-AX 00G-4457-P0-AX 00F-4472-P0-AX 00G-4472-P0-AX

00G-4459-U0-AX 00G-4457-U0-AX 00G-4472-U0-AX

250 x 50

15 x 21.2**

15 x 30.0+

00G-4459-V0-AX 00G-4457-V0-AX 00G-4472-V0-AX for ID:

/ea AJ0-8405 AJ0-8400 AJ0-8473 18– 29 mm

/ea AJ0-8406 AJ0-8401 AJ0-8474 30–49 mm

*SecurityGuard Analytical Cartridges require holder, Part No. : KJ0-4282 SemiPrep SecurityGuard Cartridges require holder, Part No. : AJ0-7220 **PREP SecurityGuard Cartridges require holder, Part No. : AJ0-8223 + PREP SecurityGuard Cartridges require holder, Part No. : AJ0-8277

‡

Bulk Media for Process Scale Purification Beyond our largest preparative column dimensions, Lux™ phases are available in bulk quantities for HPLC purification at the process, pilot, and commercial scale. The highly reproducible manufacturing process makes scaling to large scale purification extremely straight-forward.

20 µm Bulk Media Phases Cellulose-1 Cellulose-2 Amylose-2 †

26


100 g

1 kg

04G-4465 04G-4464

04K-4465 04K-4464

†

†

Please inquire for 20 µm Lux Amylose-2 media. ™

If you are not completely satisfied with Lux™ analytical columns, send in your comparative data to a similar product within 45 days and KEEP THE COLUMN FOR FREE. Use SecurityGuard™ to increase column lifetime Use Phenex™ Syringe filters for mobile phase solvent filtration

Phenex Syringe Filters ™

For Sample and Solvent Filtration Prior to Chromatography! • Less system downtime • More consistent, reproducible results • Increased column lifetime

Membrane Type/Size 0.45 µm Phenex-RC

(Regenerated Cellulose)

Phenex-PES 3

(Polyethersulfone)

Phenex-PTFE

6

(Polytetrafluoroethylene)

Phenex-NY (Nylon)

15-17 mm Diameter for 2 – 10 mL sample volumes

25-28 mm Diameter for 10 – 100 mL sample volumes

Part No.

Unit

Part No.

Unit

AF0-2103-12 AF0-2103-52 AF2-5108-12 1 — AF0-2102-12

100/pk 500/pk 100/pk — 100/pk

AF0-8103-12 5 AF0-8103-52 5 AF0-8108-12 7 AF0-8108-52 7 AF0-1102-12

Price

AF0-2102-52

500/pk

AF0-1102-52

100/pk 500/pk 100/pk 500/pk 100/pk 500/pk

AF2-5107-12 1

100/pk

AF0-1107-12

100/pk

AF2-5107-52

1

500/pk

AF0-1107-52

500/pk

AF0-2203-12 AF0-2203-52 — — AF0-2202-12 AF0-2202-52 AF2-5207-12 1 AF2-5207-52 1

100/pk 500/pk — — 100/pk 500/pk 100/pk 500/pk

AF0-8203-12 5 AF0-8203-52 5 AF0-8208-12 7 AF0-8208-52 7 AF0-1202-12 AF0-1202-52 AF0-1207-12 AF0-1207-52

100/pk 500/pk 100/pk 500/pk 100/pk 500/pk 100/pk 500/pk

AF0-8515-12

7

100/pk

AF0-8515-52

7

500/pk

Price

0.20 µm Phenex-RC

(Regenerated Cellulose)

Phenex-PES

3

(Polyethersulfone)

Phenex-PTFE

6

(Polytetrafluoroethylene)

Phenex-NY (Nylon)

1.20 µm Phenex-GF

2,3

(Glass Fiber)

Prefiltration of heavily contaminated or highly viscous samples. When used in-series preceding a membrane filter, clogging of the membrane filter is prevented and sample clean up is optimized. Outlet connection is luer lock.

A special thank you to PDR-Chiral for supplying preparative applications on page 25.

Advanced Laser Polarimeter (ALP) Simplify your HPLC, SFC, and Bulk purification Measures the real-time rotation of a plane-polarized 670 nanometer laser beam as it passes through the flow cell to microdegree accuracy/resolution • Universal Optical Activity Detector • No Chromophore Required and No Absorbance Anomalies • Broad Applicability: Pharmaceuticals, Carbohydrates, Antibiotics, Proteins, Foods, Flavors, Pesticides, etc. • Good Sensitivity & Reproducibility, Large Dynamic Range • Fully Automatic, No User Adjustments or Stop Flow Scans • Consistent Assignment (+/-) for Enantiomers For further information on the Advance Laser Polarimeter, please contact PDR-Chiral, Inc.

Email: [email protected] Web: www.pdr-chiral.com

If Phenex™ Syringe Filters do not perform as well or better than your current syringe filter product of similar membrane, diameter and pore size, send in your comparative data within 45 days and keep the Phenex products for FREE! Syringe filters are non-sterile. Housing is made of medical-grade polypropylene (PP) and offer luer lock inlet/slip outlet connections, unless otherwise indicated. 1. 17 mm diameter. 2. Glass fiber filters are 28 mm diameter and made of borosilicate. They will remove 90 % of all particles >1.2 µm. 3. Housing material is methacrylate butadiene styrene (MBS) polymerisate. Also known as Cyrolite™. 4. Cellulose acetate is surfactant-free. 5. 26 mm diameter. 6. Hydrophobic membrane. Can be made hydrophilic by pre-wetting with IPA. 7. 28 mm diameter. 8. Additional dimensions and membrane types are available. Please contact your local Phenomenex technical consultant or distributor for availability or assistance. 9. Larger quantity purchases at significant savings are available. Cyrolite is a trademark of CY/RO Industries. Phenex is a trademark of Phenomenex, Inc.

Australia t: 02-9428-6444 f: 02-9428-6445

[email protected] Austria

t: 01-319-1301 f: 01-319-1300

[email protected] Belgium

t: +31 (0)30-2418700 f: +31 (0)30-2383749

[email protected] Canada

t: (800) 543-3681 f: (310) 328-7768

[email protected] Denmark

t: 4824 8048 f: 4810 6265

[email protected] France

t: 01 30 09 21 10 f: 01 30 09 21 11

[email protected] Germany

t: 06021-58830-0 f: 06021-58830-11

[email protected] Ireland

t: 01 247 5405 f: +44 1625-501796

[email protected] Italy

t: 051 6327511 f: 051 6327555

[email protected] Luxembourg

t: +31 (0)30-2418700 f: +31 (0)30-2383749

[email protected] Netherlands

t: 030-2418700 f: 030-2383749

[email protected] New Zealand

t: 09-4780951 f: 09-4780952

[email protected] Puerto Rico

t: (800) 541-HPLC f: (310) 328-7768

[email protected] United Kingdom

t: 01625-501367 f: 01625-501796

[email protected]

All other countries: Corporate Office USA t: (310) 212-0555 f: (310) 328-7768 [email protected]

www.phenomenex.com

BR67170109_I_1

Phenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at [email protected]