Conservation

Conservation: 3b60_tmd_3 gi_298293017_ref_YP_00369 gi_90422317_ref_YP_530687 gi_163737496_ref_ZP_02144 gi_114320526_ref_YP_74220 gi_114706019_ref_ZP_01438 gi_91976040_ref_YP_568699 gi_84685326_ref_ZP_010132 gi_221640363_ref_YP_00252 255764467 gi_304393004_ref_ZP_07374 gi_310814550_ref_YP_00396 gi_99077981_ref_YP_611240 gi_121602516_ref_YP_98958 254780917 2hyd_tmd_3 254780193 gi_254473364_ref_ZP_05086 gi_190891096_ref_YP_00197 gi_299133618_ref_ZP_07026 gi_146341592_ref_YP_00120 254780576 Consensus_aa: Consensus_ss: FIGURE S4

1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1

5 5 6 6 8 5 6 5 5 5 5 8 5 5 5 5 5 -------------------------------------------------------WQTFRRLWPTI-----APFKAGLIVAGIALILNAASDTFMLSLLKPLLDDGFGKTD------------RSVLLWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRRRLFGHMMGMPVAFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMMFYY-SWQLSIILVVLAPIVSIAIRVVSKRFRSISKNMQNTMGQVTTSAEQMLKGHKEVLIFGGQEVETKRFDKVSNKMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM--DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFAILDSEQEKD------------------------------------------------------------------------------------------------------MTQAADATPTATTSRRP-----RLSPLMALWPHI-----RRYRGRAGAALVALVVAAVATLVVPIAVRRMIDFGFDERH------------AGFIDRYFGVMIGVVAVLAVASAARYYLVMTLGERIVADLRSQVFAHVLTLDGGFYDSAKSGELISRLTADTTQIKSAVGASASIALRNLVLFFGAAVMMVIT-SPWLSATLLIAIPAIVAVLLGAGRGVRKRSRSAQDTLAEASAYAVEAIGAVRSLQANTAEGAASRHFAGEVEHSFNAASDAVRARAWLTGFAIFLIFASVVGILWAGANAVLA--GTMTAGTLGQFVLYAVLAASGLGQLGEVWGEISQAAGATERIAELLAERPKVARPADPLPLPEPPQGSVTFANVAFAYPTRPDLPALSDVSFTVQPGERVAVVGPSGAGKSTLF---------MIQKVRPDTKSKGTVIALSLEAIAESEKVIPAGRPAPKAEPLTDAAAKPAAKHGRLRPLLLLAPYV-----DRYRWRATFAFVALTVAAATTLLVPLAVRRLIDFGFSPEG------------IALINSYFLVMVLIVAVLAMASAARYYLVMTIGERIVADLRHDVFAHLTRLSPSFFDSARSGELVSRLTADTTQIKSAVGASVSIALRNMMLFIGAASMMVIS-SPRLSGFVLAAIPLIVLPLVGFGRWVRRLSRNAQDTLADASAYASELVSSIRTVQAYTNEGLSNARFGREVEQAYEAARSSTGARAVLTAIIIFIVFSSVVAILWVGSHDVVS--GEISAGRLSQFVMYAAFAAAGLGQLSEVWGEVAGASGAAERLFEILRVKPDIAAPASPRAFPVPPRGDVVFDKVSFA----------------------------------------------------------------------------------MAGNQAVPTADVEREKSRKVGVLSALWPFM-----RPYRVLMAMATLALVLTAGLSLTLPLAVRRVVDNFRISE-------------AALLNQYFAAALVIAALLAVGTGLRYALVTRLGERVVADIRKAVFDRVIGMSPAFFEQIMTGEVLSRITTDTTLIQSVLGSSVSIALRNMLIFIGGLVLMLLT-SAKLTSLVLLIVPAVIVPILVLGRRLRKISRENQDWIAASSGNAGEALGAVQTVQAYTHEVASRSEFSRLTETAFDVSMRRIQTRAFLTVIVIFLVFSGVVGVLWMGANDVRA--GIMSEGTLVQFVIYAVLVAGSVAALSEIWSELQRAAGATERLVELLTAVDQVQDPYAPRALAAPVRGEIRFDDVSFRYPARPQIPALVDVSLTVQPGETVAFVGPSGAG-------------------------------------------------------------MTVAGPKRLEALRPLLAFL-----RRYRLRVTAAVAALLVAAGSVLTLGQALRLVVDRGFLGED------------PAELNRLLLLAMTVILIMALASALRYYLVSWIGERVAADLRTAVFDHVTHLEPAFFELNGVGEIQSRLTTDTSVLQTILGSSFSIAVRNLLLLAGAVVMLFIT-SPRLTAFVLLSLPLVLIPILYFGRRVRRLSRASQDRVADVGSYAGETLHAIRTVQAFGHEAADRRRFRRHVDAAFATAVRRVRQRAWLVGMAMMLAFTAVGIILWQGGHDVLA--GRMSAGELAAFVFYAVLAAGATGAVSEVVGELYRAAGATERLTELLRNQPRIRAPARPVPPPQPLRGAISLEGVAFAYPGRPQQRVLDRVDLRVAAGERVALVGPSGAGKSTVFALL-----------------------------------------------------MAKKRKKLKSGKALMTEKI-----GRYRGLAIFLLLLSGVINVLALTGAFYMLQIYDRALTSGS------------VPTLVAISVLAIGLYLFQGFFDVLRSQILIRFGARMDARLAPLAHEVVVEMP----RYGYSTTEALERGRDVDTVRNFVASPAPGALFDLPWMPLFLIFVFML-HPLLGLLTCAGALVLIALTTLTGALTKNSAAEVQKAAVQRGNLADCHARNADILRAMGFAGRAVARFEAANEKHLQLQTRTSDISGSFAGLSKVLRMMLQSALLGLGAYLTIQ--GDLSAGAIIACSVASARALAPIDVILGQWKNMVSARRSFDRLQETIEAMDDDS---KFLELP-APTKSIKVENITIVAPAT-GAILVSDVSFEVKAGQALGLIGPSGGGKTSLAKG------------------------------------------------------MAKPKELSSAKRRNLAELT-----RGLRGVAIFLFLVSGMINILALTGSLYMMQIYDRALTSGS------------IPTLLLLSALAAGLYLFQGCFDVIRSQVLVRVGARLDKKIAPLAHRVAIDMP----RFGFSTSEALERGRDVDTVRGFLGSQGPSALFDLPWMPIYLVFIYFL-HPLLGALTFAGAFVLTIMTVVSEVLTKRLSIATRKAAMARNTIADCNARNAEVLKAMGFADRAVSRFNIANGEHLELQTKANDITGNFAAVSRVLRMLLQSAVLGLGAYLTIK--NELSAGAIIACSVASARALAPVDLAIGNWKSFIAARMAYHRLRDTVVALASAD---EPMQLP-PPKNRLTVEKATIAVPGT-GQIVLSDVSFELEAGQALGVIGPSAGG---------------------------------------------------------------MSLDTTVSGRTELRAVL-----RENRGLFWAVGIFSVFMNVLMLTGPLYMLQVYDRVLGSRS------------EETLIALTILMAFLFAMMGILDFVRGRVLARIGAKFQAKLDHRVFTAVLDYDS--RTNRKSGNSENGL-KELEAIRRLLSSPVFGALFDLPFTPVFLAGILLF-HPYLGILALVGGGVLVTIAILNQISTSAPARQANAATMQSDNVAEQLRGEAEMVRALGMSDAAFSRWYRQRHAALADAILSADRSGAYTSLSKTLRLLLQSAMLGLGAYLVLE--GHVTPGVMIAASILLGRALAPVDLLIGQWQIVQAGRRAWRTLSALLSEVARPG---PRTALP-RPRAVLTAENLTVVPPGT-TQASLRMVNFDVQPGEALGVIGPSGAGKSTLARAITGVWNPA -------------------------------------------------MTRSDNRKGLEELRAVR-----RSSMGALTSVFLFSIFVNLLMLTGPLYMLQVYDRVLGSRS------------EETLLALSLLVTFLFVAMGVLDHVRARVMARVGAGFQEKLDRRVFEAAVRRLS----LAPGDPSAVAAQRDLESVQRLWASPVLIALFDIPWTPFFLAAIFVF-HPYMGWLAIGGGVVLVIVTILNQRLSEGPMQKANIVALQADRFAENLKSESEVVQALGMAGNGFDRWHKARAAALDANMSAADLTGAFGTLTKTLRLFLQSAMLGLGAWLVLQ--QELSAGAMIAGSILMGRALAPIESAIGQWALVQRASEGWRRLGELLTRQPVEP---PRIALP-RPRALIEAQNLSVVPPGE-AVAVLRGVSFRLDPGQALGVIGPSGSGKSTLARA--------------------------------------------------------------MYHLKYSANFM-----KIYFSFFCITILPSFFINILYLTNPLYMMHIYDSVIGTQS------------KINLIALSTITLFLYALFFGFDLIRSRLLIESSHLIERFFKPYIITVTKKYE-------MDGTSLFSIISSLDQLKQFITSPVLPALLDILFTPIFIILSFCI-HPILGSWAILSGILLLTITILFQANNERLERRYKKSRQDEINFGKAILHNSEYIYAPSTRDFLLTYWNKKRATSQEYQSLIAKKYYFGISVTKTLRMTLQSSILGIGAWLVIH--QKLSAGAIIATSIITARAMAPLEQIINSKKSLNVGLKSLKYLINLNDFTTLLN---SSDSKK--EPISMRLS---------------------------------------------------------------------------------------------------MKNPQDMGIFQRMVSENF-----SHYRRDYAWAVFCLVIMAGITAFSAWLMGPVVKDVFYGND------------MNQAVFLSSVVAAIFLVKGAVSYFQAVILNRIGNNMVARYQRRLFQHLLDLDVKFFAKQHSVSLIAQLNQNTNAVRDLLNNVVLGYARDLVTLIGLVGVMLYR-DPYLALAILVIGPIALTTLARYARRVKVIAREEVHINSQVATAMQEVAHGIPVVKAFTMEEQLRVKLDALTVKAEGRSNNIARITARTSPLMETLSGLAIAAVISYGGWRVIT--QGYEPSDLTSFMTALLLAYDPAKKLAKLRVTLERSMVNARMIYEVLDTEIASKEAEAGVDLV-MDGGAITFDNVTFSYDE----LPVEGATLRAPLP----VIRD--------------------------------------------------------MQLTQKIMSRFVKDDVDGNVVKRLLQYGV----ATQGRYYAIGILAMVIVATSAGLTAWSMEMIINAMSNPND------------RAQLATVSMMVVGIFALRGIGAYVQAVAMAKAGNGIIADQQRNIFNKLMEQGVDFFNLRESSDTLMRVTQSAQAARGLIDIIATSAVRDSLTLISLLVVMVYQ-QPVLTLAAMTVGPMAFLMLRTLVRRVRDVTSRQMMSLAEILRVLQETSAGIRIVKIFALEDLMRGRMSAAVRAVEKRSNAVTRLESITMPVMDILAGLTIAGILWLSTWNIVGGADGASAGQLMSFITAMLMCYDPARRLSQMRVRAEAMLVGVRMLFDLIDMKTSLNENPDGPALK-PGPAHIRLEDVTFAYGE---RKVL--------------------------------------------------------------------------------MIARSWDRWRSSYIGRLVSESM-----RSQGWRYGVAALAMIIVAASSAGTAWMMEKIVDAMTTPEL------------RSKVYLVAGAVIGLFFTKALASYVQALFLARAGNRIVASQQEKLYRQLLRRGVGFFGTTDSSDLLMRVTHGAQAARELIDVLMTGFVRDTLTLIGLTIVMVYQ-QPVLSMLFILIGPIALLGVRHLLKSVRNIMRAEMASLAEIIRVLQETSGGIKIIKVFALEDRMVERMDTAIGQVEKRANDIAKLQSIASPLMELLAGVAIASILVVSTMGIANS-EPPTAGQLMSFITALLMAYEPAKRLSRMRVGIETCMVTVSMMFELLDQDEAIQERAGAVNLE-PGNGSIHFNNVTFGYHS--DFPIIKDMNLSF----------------------------------------------------------------------MVKKQTS---SFDKHLITRLLRENF-----QKHVHWYSAAIVSMIIISLTTATSAWIMRDVVNHIIDAKN------------FNVIIFISGAIAFIFTLKGIATFAQTYFLSKAGNSIVAEQQRKIYARLMQQGVSFYHNNTSSDLLIRVTHNATEARIVIDTIITTFVRDLLSVIGLLFVMFIQ-NFTLMTISLTVGPLAFLGVRIALKRVRNLMEKELHSLGEIIKVMQETAIGIQVIKAFSLEELMKKRMNEAISDVEQKANSISTLEAATSPIMETLAGIAIAVVICFSGYLANQ--HAGVQGELMSFIVALLLAYEPAKRLANVRVKIESGLVSIRTMFEILDYPLTVIEHKKAKNLN-KTQHSIRFEHVSFAYKD--DQEVLNDINLEI----------------------------------------------------------------MRNFFFVLDKVMS---PSDREMLSRLIRENI-----HKHVTWYSMSIVAMITVSLMTSCSAWIMRDVMNAMVASTD------------IFHVIVVSSTVAGIFIVKGIASFVQNYYLSCAGNSIIAEQQRKIYRRLLQYGMEFYDSNQSSELQVRFTHATQSVRSVVDIFITSFLRDFLTLVGLIVVMFLQ-EPTLSLCTIIIGPLCILGVRILVKRVRHIMEQGMVAIGHIIQNLQETVIGIRIVKSFAMEEVMNQRMCNMIKEVEDRLNKVAKIESATSPIMETISGLSIAGIILFSGFLMSKK-GTSNAGEIMSFITALLMAYEPAKRIARTRIILEGGMVGVRCMFSLLDHPIMIEESPHAVNLP-VG-------------------------------------------------------------------------------------------------------------------MIKRYLQFV-----KPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGVINNHALTTDE-----KVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYNHLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFL-DVKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAIEDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAIS--GSITVGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE-------------------------------------------------------------MVNKI--------------------------------------THKNL----NENISLFKFQILYL-----RHCWWFLTGELCAILLYSGITLLIPVMTSVLLDKRGVSPLMTSV------FLDKRDVSLYLWGMFVLLLLAVASSLRYYCATMLGERMILFMQRDVIAKIIKLSPSFFNSRGHGEIFSILVKDTERIKAIIGIGMSIVLRSLLMGFGAMCMMFVI-SIKLSLIVVSIVTLFLMVMIKFS---RKKTNTLYADEKKLLNIFSDMIKSVMVLQSFNAGKEAIRLYDIQSQKTYKGFLRSALIRTCIACFVIFFVSCGIFIVFLIGAHYVAT--AEMPRGKLAEFVVYAFIFFASIRSCLGFIGGISQAIASLRRLRELIVCKLDISSPISSRTFP-SP---------------------------------------------------------------------------------------MTATSKKQQEKSAPALDADD---KGLLRSLKTLWVYMWPDARPELKKRVVLALAALIVAKIITVFAPYLYAWATDALTDNSVLPNWLP----PILIAPIMLVIAYNVARTMAVGFNQIRDAIFSSVGLHAVRELANLTFQHLHALSLRYHLSRRTGGLTRVIDRGVKGIEGIVRFTILSGIPTVVEFAMMAVVIWFQFGFWYVVVVGVTVFAYVYYTVVASDKRIQIRRDMNNADNDANSKSVDSLLNFETVKYFGNEEMERGRFDKAMRGYERAAIKTWTSLSWLNFGQALILGVGMTTCMVMSAMAIQR--GEQTIGDFVMINALLMQLSVPLNFIGFIYREIRQGIADLEAMFSVLAVEPEIQDEPLAENLK-VKEATIKFEDVHFHYDE--ERPILKGVNFEVPAG------------------------------------------------------------------MANGKTVSE---SHLLRTLLNLWPYMWPAGRPDLKMRVVWASVYLLISKFVLLLVPYFFKWSTDALNGRMDLAGTVP----PLLAGAIALVIAYNLTRLIQLGLNQLRDALFASVGQHAVRQLAYRTFVHMHELSLRFHLERKTGGLSRIIERGTKGIETIVRFTILNSVPTVIEFLLTAAIFWWGYGFSYLAVTALTVWAYIWFTIRASDWRIAIRRSMNDSDTDANTKAIDSLLNFETVKYFGNEEMEAKRFDKSMERYEKAATDVWTSLGWLNFGQGVIFGIGTTIMLVLSALAVQR--GEQTVGDFVFVNSMLLQLSAPLNFIGFVYREIRQGLTDIEQMFDLLEVKAEVKDAPDATELR-SGQGAISFKDVHFAYD---------------------------------------------------------------------------MTASDTPDQNRPARAVAN---ASLTATLVQLWPYIWPGERADLKMRVVWSMVLLVIAKALTMIVPFTFKWAIDALNGQGSAPVAPSNWMMWLLASPIILTVSYGATRVLMAVFTQWRDGIFAKVAMHAVRKLAYLTFVHMHELSLRFHLQRKTGGLTRVLERGRTGIETIVRLAVQQLAPTVVELTMMMGVLLYQFDWRYVLITVGTVLAYMWFTYVATEWRIDIRRRMNESDTEANTKAIDSLLNYETVKYFGAETREAQRYDASMERYERASTKTYTSLAWLNTGQAVIFSFGITATMVLCAIGIRN--GTQSVGDFVMINAMMIQLYQPLNFMGFVYREIKQAIIDIEMMFGVLSRNPEVKDRPGAKPLQ-VTSGTVRFEDVHFHYDP--DREILKGITFEVP-----------------------------------------------------------MMPPDSSPHHLATDAPASPEQGTLAGTLRHLWPFIWPSDRADLKMRVLWSVVLLLAAKVATLVLPFTFKWTTDALTGANTAPIKPDDWAFWLVASPIVMTASYGAMRVLMAVLTQWRDGIFARVAMHAVRKLATITFVHMHELSLRFHLERKTGGLTRVLERGRNAIETIVRMVILQLIPTIVEVSLLTAVLLWQFDWRYVLVTLTMVGAYLFYTYIATEWRIEIRRRMNDSDTDANTKAIDSLLNYETVKYFGAEAREAERYDRSMERYERASVKTYTSLAVLNAGQAVIFTIGLTIAMLMCAHGVRQ--GRNTVGDFVMINSMMIQLYQPLNFMGMVYREIKQAIIDIEKMFGVLTRDAEVKDAPRAKPLV-VTAGHVRFEDVRFAYEP--ERPILQGLS----------------------------------------------------------------------------MAFAR---FPSLRTLKKLWPYMWPANRWDLKVRIIGAMFSVIASKFVILGIPFLLKWVTESLIEQSTASKTTS----YLTIGIVILITSYGTMRVVNLISNHIRDFLYVEIGQGATCTLYHQVVSHVYKLSQRFHINYKVGKLSSAISNGIRSIEMIIRIIVVHLIPTILEFAISMAFLWYSYGDIYVSIMAVTVILYVWFTIITSNWRVGLSKKMNNLLHECHAKVFDTLINFETIQYFNSEKFEINRLEKYISKYKESAISVATSLGWLNFGQGLIFSIGMVITMIISSHAVYI--GEQTVGDFVFINTLLTQLAIPLNILGTIYRDSRQSFIEIEELLNILNEKIEIQNAVNSKDLK-IK----------------------------------------------------------..........................................................h..hh..h.......h...hhhhhlh.hh.....hh.shhh..hhc.hh...s...............hhhh..hhh.hhhh.shhs.hp..hh..ht..h..ph...h@..hhphs..F@.p..stp....l.pshp.lc.hl...h..hl.shh.h..hhhhhhh..p..hshhhhh.s.hhhh.h.hh....p...pp...s..p....h.p.h.shphlphhs.......chp.....hbp...p.hp..thhssh..hl..h..s.hh.htth.h.......osG.hh.h.hhhh.hh.shp.h......hp.t..shc.h.plls..........s.sh.......lphp.................................................... 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