Page 1. n o n e. P. /O. = 1 q. (g lc)/q. (O. 2. ) = 2. /3 q. 0. 2 m a x. = 1. 1 .5 q. 0. 2 m a x. = 1. 4 .7. 5 m a in te n a n c e b o u n d s. N. A. D. P. H a ll c o n s tra in ts n o.
anaerobic, NO3 R1 R2 R3 R4 R5 R6 R7 R8 R9 R10
R1 R2 R3 R4 R5 R6 R7 R8 R9 R10
electron acceptor present
aerobic R1 R2 R3 R4 R5 R6 R7 R8 R9 R10
anaerobic
Computational split ratio
max ATP max ATP per flux unit max biomass per flux unit
none P/O=1 q(glc)/q(O2) =2/3 q02 max = 11.5 q02 max = 14.75 maintenance bounds NADPH all constraints
min ∑ v2 none P/O=1 q(glc)/q(O2) =2/3 q02 max = 11.5 q02 max = 14.75 maintenance bounds NADPH all constraints
none P/O=1 q(glc)/q(O2) =2/3 q02 max = 11.5 q02 max = 14.75 maintenance bounds NADPH all constraints none P/O=1 q(glc)/q(O2) =2/3 q02 max = 11.5 q02 max = 14.75 maintenance bounds NADPH all constraints
none P/O=1 q(glc)/q(O2) =2/3 q02 max = 11.5 q02 max = 14.75 maintenance bounds NADPH all constraints
additional constraint
max biomass
Objective / constraint combination
Supplementary Information Figure 5 Specific agreement ρ between computational and experimental split ratios for the five objective functions considered in Figure 3 and 5 and the three batch cultures. In vivo results are compared to computational predictions and the specific agreement indicating the individual accuracy is quantified. Increasing shading signifies increasing agreement, i.e. black is a perfect fit. Split ratios, experimental conditions and objective/constraint combinations are indicated on the x- and y-axis, respectively.
