occurrence of CFP in the Philippines is anticipated as a consequence of global ..... s ipjac tuna; 3, Spanish mac erel; 4, barracuda; 5, blue marlin; 6, red snapper; ...
Journal of Environmental Science and Management 50-55 (Special Issue 1-2013) ISSN 0119-1144 2 2
ABSTRACT
gastrointestinal and cardiac symptoms, and in some cases, chronic neurologic symptoms. In the Philippines, diagnosis
experienced temperature reversal sensation. Cigua-Check Kit screening done by the Bureau of Fisheries and mass spectrometry, which showed masses corresponding to those of CTX-1 and CTX-3B/3C. Since an increased occurrence of CFP in the Philippines is anticipated as a consequence of global climate change, validation of CFP by other methods like mass spectrometry will be useful for more effective diagnosis and management of CFP. As
INTRODUCTION Lewis 1986
Gambierdiscus
Figure 1
Bagnis et al. 1979 Swift and Swift 1993
MATERIALS AND METHODS
ciguatoxins (CTX). (A) P-CTX-1, (B) C-CTX-1 (Adapted from Lewis 2001).
Lutjanus campechanus maya-maya
Bottein et al.
Poli et al. 1997 Pocsidio and Cabrera 1999
RESULTS Cephalopholis aurantia Montojo et
Detection of Ciguatera poisoning
DISCUSSION
Bagnis et al. 1979
Glaziou and Martin 1993
Figure 2
2 2
2
SerK001 #11 RT: 0.15 AV: 1 SM: 11B T: + p ESI Full ms [850.00-1300.00]
NL: 2.74E4 1123.67
100 95 90 85 80
1023.67
75 70
1223.53
Relative Abundance
65 60 55 50 45 40 35 924.00
30 25 20 15 10 5
868.93
902.80
962.87
1037.40
1085.53
1160.00 1193.47
0 900
950
1000
1050
1100
1150
1200
m/z
Figure 2. Electrospray mass spectra of extract from blood sample collected from patient who was poisoned upon the ingestion of red snapper (Latjanus campechanus, locally known as mayamolecule (m/z = 18) is a characteristic fragmentation pattern for CTX-1 (Caillud et al. 2010, Chinain et al. 2010).
