Unique Research Journal of Medicine and Medical Sciences Vol. 4(5), pp. 038-050, September, 2016 Available online@http://www.uniqueresearchjournals.com/URJMMS ISSN 2333-6935 ©2016 Unique Research Journals
Unique Research Journal of Medicine and Medical Sciences
Full Length Research Paper
Determination of 25-OH vitamin D in human serum using the enzyme immunoassay method Olasehinde Oluwaseun Ruth1, Owoseni Joseph Sina2*, Akawa Oluwole Bidemi1 and Akintehinse O. V.1 1
College of Medicine and Health Sciences, Afe Babalola University, Ado Ekiti, Ekiti State, Nigeria. 2 Sociology Department, Ekiti State University, Ado Ekiti, Ekiti State, Nigeria. *Corresponding author. E-mail:
[email protected]. Accepted 22 September, 2016
Vitamin D is a steroid hormone involved in the intestinal absorption of calcium and in the regulation of calcium homeostasis. Serum concentration of 25-hydroxyl Vitamin D is considered to be the most reliable measure of overall vitamin D status. This research work focused on the quantitative determination of the 25-OH Vitamin D in the serum of young people aged 20 to 30 years and adults aged 45 to 65 years, using Enzyme-Immuno-Assay (EIA) (a competitive protein binding assay). The results showed that 23.5%young people were deficient in 25(OH)D (
