Development and Validation of a Two-Site ... - Semantic Scholar

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(IRMA) for human atrial natriuretic .... IRMA, immunoradiometric assay,. ANF, atrial natriuretic factor, h, human; r, rat; and ...... J, Mbanya. J-C, Thomas TH, Baylis.
CLIN. CHEM. 35/6, 953-957

(1989)

Development and Validation of a Two-Site Natriuretic Factor n Unextracted PIama Helen M.

Wendy A. Retcllffe,2

Lewis,’

RIchard

Immunoradiometric

monoclonal antibody is radioiodinated, and the igG fraction of the antiserum is coated onto wells of a microtiter plate. Plasma or standard hANF99-1 26 (150 4) is Incubated with the radioligand in coated wells for 24 h. The detection limit is pg per well, corresponding to 2 pmol/L, with a working range (CV