435/233; 530/303; 435/200; 530/395; 530/400; (75) Inventors: Franz Grus, Vreden (DE); Nils Boehm,
435/189; 435/194
lngelheim (DE); Norbert Pfeiffer,
(57)
MamZ (DE); Kathanna Bell’ MamZ (DE)
The invention concerns a ?rst diagnostic method for glau coma based on an analysis of autoimmune reactivity in body
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_
ABSTRACT
?uids against at least one sample of at least partially puri?ed
(73) Asslgnee? M'LAB AG, Zolhkon (CH)
ocular antigens, Wherein the autoimmune reactivity against
(21)
Appl' NO‘:
individual antigens is measured and transformed into a glau coma score to determine the diagnostic result. Further aspects
(22)
PCT Flled'
(86)
.
13/641’009 _
of the invention include antigen carrying elements carrying at
Apr‘ 13’ 2011
least one sample of the at least partially puri?ed ocular anti
_ PCT NO"
PCT/CH11/00077
gens and kits for diagnosis of glaucoma. Further aspects include methods of collecting a body ?uid such as tears for the
§ 371 (c)(1), (2), (4) Date:
Mar. 20, 2013
use in the diagnostic method for glaucoma.Yet further aspects include ocular antigens serving as diagnostic markers and/or for preparing pharmaceutical compositions for treatment of
Related U_s_ Application Data _ _
(60)
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glaucoma. The invention further concerns a second diagnos tic method for glaucoma comprising the steps of a) providing
an in vitro culture of cells; b) incubating a body ?uid from a test individual With the in vitro culture of cells or incubating components, Which are fractionated from the body ?uid or from a body specimen of the test individual With the in vitro
culture of cells; 0) analyzing protein expression of the cells (51) (52)
and/or analyzing the viability of the cells after treatment according to step b); and d) comparing the results of the analysis in step c) With standard data to determine a diagnos tic result.
