RESEARCH ARTICLE
Differential MicroRNA Expression in Human Macrophages with Mycobacterium tuberculosis Infection of Beijing/W and NonBeijing/W Strain Types Lin Zheng1, Eric Leung1, Nelson Lee2, Grace Lui2, Ka-Fai To3, Raphael C. Y. Chan1, Margaret Ip1*
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1 Department of Microbiology, The Chinese University of Hong Kong, Hong Kong SAR, China, 2 Department of Medicine & Therapeutics, The Chinese University of Hong Kong, Hong Kong SAR, China, 3 Department of Anatomical & Cellular Pathology, The Chinese University of Hong Kong, Hong Kong SAR, China *
[email protected]
Abstract OPEN ACCESS Citation: Zheng L, Leung E, Lee N, Lui G, To K-F, Chan RCY, et al. (2015) Differential MicroRNA Expression in Human Macrophages with Mycobacterium tuberculosis Infection of Beijing/W and Non-Beijing/W Strain Types. PLoS ONE 10(6): e0126018. doi:10.1371/journal.pone.0126018 Academic Editor: Selvakumar Subbian, Public Health Research Institute at RBHS, UNITED STATES Received: December 12, 2014
Objectives The role of microRNAs in association with Mycobacterium tuberculosis(MTB) infection and the immunology regulated by microRNAs upon MTB infection have not been fully unravelled. We examined the microRNA profiles of THP-1 macrophages upon the MTB infection of Beijing/W and non-Beijing/W clinical strains. We also studied the microRNA profiles of the host macrophages by microarray in a small cohort with active MTB disease, latent infection (LTBI), and from healthy controls.
Accepted: March 27, 2015 Published: June 8, 2015 Copyright: © 2015 Zheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: The data discussed in this publication have been deposited in NCBI's Gene Expression Omnibus and are accessible through GEO Series accession number GSE65810 for the human miRNA and GSE65811 for THP-1 cells miRNA profiles (http://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE810; http://www.ncbi.nlm.nih. gov/geo/query/acc.cgi?acc=GSE811) respectively. Additional Tables and Figures are in Supporting Information (Tables S1 and S2, Figures S1, S2, and S3.
Results The results revealed that 14 microRNAs differentiated infections of Beijing/W from non-Beijing/W strains (P
