diptera: psychodidae

N° 26 (1) 2015 Edición especial

ISSN 1666-4612

Boletín de la SEA Sociedad Entomológica Argentina

Encontranos en: http://seargentina.myspecies.info/ Facebook: Sociedad-Entomologica-Argentina http://www.museo.fcnym.unlp.edu.ar/

De los Editores Estimados lectores: Este número especial del Boletín, está íntegramente dedicado al VIII International Symposium on Phlebotomine Sandflies (ISOPS VIII). Esta publicación incluye 249 resúmenes de los trabajos presentados por los participantes del simposio, que fueron sometidos a arbitraje oportunamente. Los resúmenes están agrupados en dos bloques, uno correspondiente a las mesas redondas y ponencias orales, y otro a los posters. En todos los casos se abarcan diferentes temáticas, incluyendo taxonomía, filogenia, genética, ecología, control de vectores, entre otros. Las búsquedas se podrán realizar a través de un índice temático y/o por autor. Por último se muestra una tabla con información referida a la cantidad de autores por país y a la de presentaciones según cada modalidad. Esperamos que este número sea del interés de los lectores y agradecemos a los organizadores del Simposio la elección de nuestro Boletín para publicar los contenidos del mismo. Hasta el próximo número Las editoras

Dirección y edición Nora Cabrera Fabiana Gallardo Edición y diseño Julia Rouaux

Dirección Boletín de la SEA. División Entomología, Museo de La Plata. Paseo del Bosque s/n. La Plata CP(1900), Buenos Aires, Argentina.

A los autores A todos aquellos que deseen enviar contribuciones para el Boletín por favor dirigirse previamente a Nora Cabrera: [email protected] para recibir las pautas editoriales.

Dear Friends and Colleagues, It is a great privilege for us to host and organize the VIII International Symposium on Phlebotomine Sandflies in Puerto Iguazú, Argentina. This time the ISOPS features a Keynote Lecture by Dr. Eunice A. Bianchi Galati, 8 speakers at Round Tables and 73 Oral Presentations. In addition, 249 abstracts were presented, belonging to 813 authors from 35 countries. As many of you know, because you have participated in this process, the first ISOPS took place in Rome in 1991 due to the impulse of Dr. Robert Killick-Kendrick and Dr. Michele Maroli, honoring the 300th Anniversary of the first-known illustration of a Phlebotominae sand fly by Filippo Bonanni. After this former event, ISOPS has alternated between the Old and the New World in Venezuela, France, Brazil, Tunisia, Peru, and Turkey. In 2014, this original idea reaches the eight Symposium in Argentina. After seven successful ISOPS we recognize ourselves as a growing community, but without losing the feeling of a periodical gathering of friends. Now we must preserve the heritage but also face the new challenges, and look forward with the main objective to reduce the sand fly-borne diseases burden in a changing world. New generations and methodologies are being incorporated; new voices were added to the multilateral global complexity; new knowledge and so new questions come with each ISOPS. As an ISOPS group, we can constitute a consolidated voice of sandfly researchers that could give evidencebased opinions, asked for or given to health policy makers. The consensual outcomes should include the voice of leading experts but also the amazing experience of worldwide distributed groups. As the ISOPS event, we can periodically produce a document that updates research-based evidence, gaps and priorities, beyond objectives narrowed by individual, laboratory, country or 'technological fashion' interests. As the ISOPS community, we can strength the collaborative network by searching for multi-partner projects and cross-training empowerment internships, according to the priorities defined in the ISOPS document. We can also establish common ground to define basic research protocols in order to make the results of different groups more comparable, and so achieve faster conclusions and stronger recommendations. We thought of many ways to homage the respected and beloved ISOPS friends that we have lost in the last years: Dr. Bob Killick Kendric, Dr. Italo Sherlock, Dr. Richard Titus, and our friend Dr. Alexandre Peixoto. Finally, we realized that the better way to make so is to keep the ISOPS going on and growing. Therefore, we can further discuss the ideas presented here during ISOPS VIII. We are very thankful to the Ministry of Health of Argentina, to all conference sponsors for their contributions, to the members of the Honorary and Scientific Committees for their active participation, and to you for your willing attendance. We hope you have a fruitful ISOPS, and a pleasant stay in Puerto Iguazú, one of the seven Natural Wonders of the World. On Behalf of the Organizing Committee and the Argentinean Network for Research on Leishmaniasis (REDILA)

Oscar Daniel Salomon

Maria Gabriela Quintana

Maria Soledad Santini

Honorary President Eunice A B Galati HONORARY COMMITTEE Añez Néstor Killick Kendrick Mireille Marolli Michele Özbel Yusuf Pérez Enrique Rioux Jean-Antoine Ready Paul D Shaw Jeffrey

SCIENTIFIC COMMITTEE Bates Paul Brazil Reginaldo Depaquit Jérôme Elnaiem Dia-Eldin Feliciciangeli Dora Ferro Cristina Galati Eunice Gallego Montserrat Lawyer Phillip G Léger Nicole Pimenta Paulo Rangel Elizabeth Valenzuela Jesús Volf Petr Warburg Alon Yaghoobi-Ershadi Reza

ORGANIZING COMMITTEE President Oscar Daniel Salomón

Collaborators Soraya Acardi Mariana Manteca Acosta

Secretaries

Analia Araujo

Ma. Gabriela Quintana

Alicia Paola Benitez Ibalo

Ma. Soledad Santini

Pablo Berrozpe Christina McCarthy

Treasury Nicolás Silvero

José Direni Mancini Magalí Giulinai Javier Liotta

Accreditation and Diffusion

Beatriz Oscherov

Denise Fuenzalida

Julia Portnoy

Enrique Szelag

Cristina Remondegui

Mariela Martínez

Lucrecia Villarquide

Translations Sergio Casertano

Graphic Design Claudia Nose

María Eugenia Utgés Website Design Editorial and Publications Juan Rosa María Soledad Fernández Matías Parra

Martín Abelardo

ISOPS VIII Puerto Iguazú Argentina

Content & Index

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

CONTENT ROUND TABLES AND ORAL SESSIONS

Phlebotomine Sand Flies (Diptera: Phlebotominae) in Brazil”

ID 1-O Albert Picado; Murari Lal Das; Mark Rowland; James Austin; Elisa De Lazzari. “Evaluation of Different Nets Against Phlebotomus Argentipes, The Vector of Visceral Leishmaniasis In Nepal” ID 2-O Rajesh B Garlapati, Sachidananda Samantaray, Mutum Ingobi Singh, Trey Barresi, Dylan Burruss and Richard Poche. “Multiple Fipronil Doses in Dairy Cattle for Vector Control in Bihar, India: Residue Study and Efficacy Determination Against the Sand Fly Phlebotomus Argentipes” ID 3-O M Derbali, L Polyakova, A Boujaâma, D Burruss, S Cherni, W Barhoumi, I Chelbi, R Poch, E Zhioua. “Laboratory and Field Evaluation of Rodent Bait Treated with Fipronil for Feed Through and Systemic Control of Phlebotomus papatasi”

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ID 14-O V Carter, DP Bray, G Borges-Alves, E Dilger, O Courtenay, R Brazil and JGC Hamilton. “Sex Pheromones as a Pre-Mating Species Isolation Barrier in Brazil” ID 15-O Chukwunonso Onyemaechi Nzelu, Abraham G Cáceres, Ken Katakura, Yoshihisa Hashiguchi and Hirotomo Kato. “Identification of Peruvian Sand Fly Species (Diptera: Psychodidae) through DNA Barcodes” ID 16-O Randrianambinintsoa Fano José; Léger Nicole; Boyer Sébastien; Robert Vincent; Depaquit. Jérôme. “Origins and Affinities of Malagasy Phlebotomine Sandflies” ID 17-O Israel Cruz, Rosa Gálvez, Oihane Martín, Ivonne Pamela Llanes-Acevedo, Carolina Arcones, Rocío Checa, Ana Montoya, Carmen Chicharro, Silvia Miguelañez, Guadalupe Miró. “DNA Barcoding of Mediterranean Phlebotomine Sandflies Based on the Sequence Analysis of the Mitochondrial Cytochrome Oxidase I Gene”

ID 4-O Mehmet Karakuş; Samiye Demir; Hüseyin Çetin; Suha Kenan Arserim; Önder Ser; Seray Töz; I Cüneyt Balcioğlu; M Kirami Ölgen; Bahtiyar Yilmaz; Yusuf Özbel.“Natural Infection and Insecticide Susceptibility Status of Wild Caught Sand Flies in Rural Areas of Antalya, Mediterranean Region of Turkey”

ID 18-O Jérôme Depaquit, Maria Dolores Bargues, Santiago MasComa. “A Molecular Phylogeny of the Phlebotominae”

ID 5-O David M Poché, William E Grant, Hsiao-Hsuan Wang. “Sand Fly Control in Bihar, India: Modeling Alternative Control Strategies Using Systemic Insecticides Orally Administered to Livestock”

ID 19-O Aysheshm Kassahun, Kristýna Hlavačková, Vít Dvořák, Asrat Hailu, Alon Warburg, Jan Votýpka and Petr Volf. “Distribution, Phylogeny and Taxonomy of Sergentomyia in Ethiopia”

ID 6-O Diwakar Singh Dinesh, Faizan Hassan, Vijay Kumar and Pradeep Das. “Development of Resistance Against DDT to Phlebotomus argentipes (Diptera: Psychodidae) and Search for Alternative Insecticide”

ID 20-O Vit Dvořák, Jérôme Depaquit, Valentin Pflüger, Petr Halada, Kristýna Hlavačková, Veronique Lehrter, Jorian Prudhomme, Sonja Zapata, Petr Volf, Alexander Mathis. “Rapid Identification of Phlebotomine Sand Flies by Maldi-Tof Mass Spectrometry”

ID 7-O Erika Santamaría, Olga Lucía Cabrera, Raul H Pardo. ”Toxic and Behavioural Effects in Experimental Huts of Long Lasting Insecticide Treated Nets Against Lutzomyia Longiflocosa in the Sub-Andean Rural Zone of Colombia”

ID 21-O Vit Dvorak, Ozge Erisoz Kasap, Jerome Depaquit, Bulent Alten, Jan Votypka, Petr Volf. “Two New Species of the Subgenus Transphlebotomus Revealed by Molecular Taxonomy”

ID 8-O Waleed Al Salem, Louise Kelly-Hope, Maha Abdeladhim, Salah Balgonaeem, Mohammed Al Zahrani, Jesús Valenzuela, Álvaro Acosta-Serrano. “A control strategy for Old World cutaneous leishmaniasis outbreaks”

ID 22-O Ivonne Pamela Llanes-Acevedo, Carolina Arcones, Rosa Gálvez, Oihane Martín, Rocío Checa, Ana Montoya, Carmen Chicharro, Silvia Miguelañez, Susana Cruz, Guadalupe Miró, Israel Cruz. “DNA Sequence Analysis Suggests that Cytb-Nd1 Pcr-Rflp may not Be Applicable to Sandfly Species Identification in the Whole Mediterranean Region”

ID 9-O Raúl H Pardo, Olga Cabrera, Catalina Marceló, Erika Santamaría. “Long Lasting Insecticide Treated Nets Decrease Sandfly Abundance and Blood Feeding Success Indoors in the SubAndean Region of Colombia: Results of a Hamlet Vector Control Trial after One Year Follow Up” ID 10-O Olga Lucía Cabrera, Erika Santamaría, Raúl H Pardo. “First Experimental Hut to Study The Effect of Insecticide Control Measures Against Sandflies (Diptera: Psychodidae)” ID 11-O Manteca Acosta M; Molina J; Utgés M E; Mastrangelo AV; Pérez AA; Santini M S; Salomón OD. “Efficacy Of Impregnated Bednets And Species Composition In Experimental Henhouses” ID 12-O Domenico Otranto; Filipe Dantas-Torres; Emanuele Brianti; Fabrizio Solari Basano; Dorothee Stanneck; Katrin Deuster.“Prevention of Canine Leishmaniosis in Naturally Exposed Dogs Using a 10% Imidacloprid/ 4.5% Flumethrin Collar (SERESTO®)” ID 13-O Andrey J de Andrade, Paloma H F Shimabukuro, Eunice A B Galati. “A New Proposal for the Taxonomic Identification of

ID 23-O Reale S, Torina S, Zaffora G, Cosenza M, Pitti R, D'Agostino R, Intile S., Piazza M, and Vitale F. “Multilocus Real Time PCR Identification of Phlebotomine Sand Flies Captured in Southern Italy” ID 24-O Nicole Léger and Jérôme Depaquit. “The Genus Idiophlebotomus (Diptera; Psychodidae)” ID 25-O Añez Néstor, Rojas Agustina, Yépez José Yancarlos. “Sand Fly Species Transmitting Visceral Leishmaniasis at the Semiarid Region of Venezuela” ID 26-O Luis G. Estrada, Horacio Cadena, Edgar Ortega, Luz A Acosta, Andrés Vélez-Mira, Rafael J Vivero, Eduar E Bejarano, Sandra Uribe, Iván D Vélez. “Natural Breeding Sites of Lutzomyia Evansi (Diptera: Psychodidae) in the Urban Zone of Ovejas, Sucre – Colombia” ID 27-O Perez JE, D Rado, D Quispe, A Quispe, F Toccas, M Chacon, B Valencia, A Llanos, M Quispe, E Aguilar, R Pacheco & E Ogusuku. “Lutzomyia Faunas (Diptera: Psychodidae) in the East Side if the

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

Andean Cordillera in Cuzco, Peru”

Özbel, P Volf, B Alten. “Phlebotomine Sand Flies Crossing Anatolia: Do Borders Limit their Distribution?”

ID 28-O Karina Mondragon-Shem; Waleed Alsalem; Louise KellyHope; Maha Abdeladhim; Mohamed Alzahrani; Ziad Memish; Jesus Valenzuela; Alvaro Acosta-Serrano.”Differential Exposure to Sand Fly Bites in Saudi Arabia” ID 29-O Paulo Silva de Almeida, Alan Sciamarelli, Josué Raizer, Jaqueline Aparecida Menegatti, Sandra Cristina Negreli Moreira Hermes, Maria do Socorro Laurentino de Carvalho, Andrey José de Andrade, Rodrigo Gurgel-Gonçalves. “Predicting the Geographical Distribution of Leishmaniasis Vector Species in Central West Brazil” ID 30-O Raquel Gonçalves, Daniela Cristina Soares, Ricardo Guimarães, Walter Souza Santos, Gilberto Cesar de Sousa, Anadeiva Portela Chagas, Lourdes Maria Garcez. “Ecology of Sandflies in Risk Zones for Tegumentary Leishmaniasis Transmission in Santarém Municipality, Western Pará State, Brazil” ID 31-O Dia-Eldin A Elnaiem. “Phlebotomus Orientalis and the Fall and Rise in Visceral Leishmaniasis in East Africa” ID 32-O Bilel Chalghaf, Amine Toumi, Sadok Chlif, Jihene Bettaieb, Mariem Harrabi, Goze Bertin Bénié, Afif Ben Salah. “Distribution of Leishmaniasis Vectors in the Mediterranean Basin in the Light of Climate Change” ID 33-O Aviad Moncaz, Oscar Kirstein, Araya Gebresellassie, Wossenseged Lemma, Solomon Yared, Teshome Gebre-Michael, Asrat Hailu, Moshe Shenker and Alon Warburg. “Characterization of Breeding Sites of Phlebotomine Sand Flies in the Judean Desert and North-West Ethiopia”. ID 34-O Oscar David Kirstein, Araya Gebreselassie, Aviad Moncaz, Asrat Hailu, Teshome Gebre-Michael and Alon Warburg. “Fine Scale Analyses of Factors Contributing to the Transmission of Visceral Leishmaniasis in North Ethiopian Villages: An Eco-Epidemiological Approach” ID 35-O Wossenseged Lemma, Habte Tekie, Meshesha Balkew, Teshome Gebre-Michael3, Alon Warburg, Asrat Hailu. “SeroPrevalence L. donovani in Labour Migrants Population dnd Entomological Risk Factors in Extra-Domestic Habitats of MetemaHumera Lowlands - Kala-Azar Endemic Areas in the Northwest Ethiopia” ID 36-O Araya Gebresilassie1, Ibrahim Abbasi2, Oscar David Kirstein2, Aviad Moncaz2, Habte Tekie1, Meshesha Balkew3, Alon Warburg2, Asrat Hailu4, and Teshome Gebre-Michael3 “Host Preference Patterns of Phlebotomus (Larroussius) orientalis Parrot, 1936 (Diptera: Psychodidae) in the Visceral Leishmaniasis Endemic Area of Tahtay Adiyabo District, Northern Ethiopia” ID 37-O Esayas Aklilu, Habte Tekie, Meshesha Balkew, Alon Warburg, Asrat Hailu, Teshome Gebre-Michael. “Bionomics of Phlebotomine Sandflies in a Highland Kala-Azar Focus in LiboKemkem District, Northwestern Ethiopia”

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ID 40-O M R Yaghoobi-Ershadi, M Darvishi, F Shahbazi, AA Akhavan, R Jafari, M Khajeian, H Soleimani, H Darabi, M H Arandian. “Epidemiological Study on Sand Flies in an Endemic Focus of Cutaneous Leishmaniasis, Bushehr City in Southwestern Iran” ID 41-O Jeffrey Jon Shaw. “Eco-Epidemiology of Leishmaniases: Vectors and New Scenarios of Transmission.” ID 42-O Alon Warburg. “Emerging Cutaneous Leishmaniasis in Israel: A Review of The Last Two Decades” ID 43-O Ns Singh; Doris Phillips-Singh; Ipe M Ipe; Shubham Pandey; Dinesh Lal. “Studies on the Sand Fly Fauna (Diptera: Psychodidae) in High-Transmission Areas of Visceral Leishmaniasis in North India, Special Emphasis on Uttaranchal Region” ID 44-O W Fares, RN Charrel, K Dachraoui, L Bichaud, W Barhoumi, M Derbali, S Cherni, I Chelbi, X de Lamballerie, E Zhioua. “Infection of Wild Sandflies Collected from Different BioGeographical Areas of Tunisia with Phleboviruses” ID 45-O Bongiorno G, Lisi O, Severini F, Vaccalluzzo V, Khoury C, Di Muccio T, Gradoni L, Maroli M, D'urso V, Gramiccia M, “Investigations On Sand Fly Bionomics and Leishmania Natural Infections in Eastern Sicily, Italy, with Particular Reference to Phlebotomus Sergenti” ID 46-O Alveiro Pérez-Doria, Margaret Paternina, Lily Martinez, Luis Paternina, Daniel Verbel-Vergara, Suljey Cochero, Eduar E. Bejarano, “Natural Infection of Lutzomyia Cayennensis Cayennensis (Diptera: Psychodidae) with Leishmania (Viannia) spp., Potential Vector in the Enzootic Cycle of Leishmaniasis in Urban And Rural Habitats of The Colombian Caribbean” ID 47-O Paul D Ready “Priorities for Progressing from Assessments of Vector Competence to the Transmission Modelling and Control of Leishmaniasis” ID 48-O Paulo F P Pimenta, Vanessa C Freitas, Nágila F C Secundino. “Leishmania Chagasi in its Natural Vector Lutzomyia longipalpis.” ID 49-O Jovana Sadlova, Veronika Hrobarikova, Jan Votypka, Alon Warburg and Petr Volf. “Xenodiagnosis of Leishmania donovani Infections in Asymptomatic Mice Using Phlebotomus orientalis Sand Flies ID 50-O V Seblova-Hrobarikova, J Sadlova, S Carpenter, P. Bates and P Volf. “Development of Leishmania Parasites in Biting Midges, Culicoides nubeculosus and Culicoides sonorensis (Diptera: Ceratopogonidae) and Implications for Screening Vector Competence”

ID 38-O Solomon Yared, Meshesha Balkew, Alon Warburg, Asrat Hailu and Teshome Gebre-Michael. “Species Composition, Abundance and Seasonal Dynamics of Phlebotomus Species in a Visceral Leishmaniasis Endemic Area of Northwest Ethiopia”

ID 51-O Luis Roberto Romero, Jorge Luis Rodríguez, Alveiro PérezDoria, José G. Vergara, Ana Montesino, Lily Paola Martínez, Luis Cortés, Suljey Cochero & Eduar Elías Bejarano. “The Sand Fly Lutzomyia evansi (Diptera: Psychodidae), as a Permissive Vector of Leishmania Infantum and Le. braziliensis In Bolívar, Colombia”

ID 39-O O Kasap Erisoz, A Belen, C Alkan, F Gunay, V Dvorak, K Ergunay, S Aydın, J Votypka, A-L Banuls, R Charrel, A Özkul, Y

ID 52-O Ana Clara Araújo Machado Pires, Paulo Filemon Paolucci Pimenta and Nágila Francinete Costa Secundino. “Experimental

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

Susceptibility of Lutzomyia (L.) Longipalpis to Different Species of Leishmania”

ID 65-O Anayansi Valderrama Cumbrera, Mara Garcia Tavares, Ingrid Murgas, José Andrade Dilermando. “Genetic Assessment of Sandfly Populations in Panama”

ID 53-O Shaden Kamhawi, Fabiano Oliveira and Jesus Valenzuela “The Prospect for a Sand Fly Saliva-Based Vaccine Against Leishmaniasis in Humans” ID 54-O Pitaluga AN, Tinoco B, Di Blasi T, Silva AM, Tempone A, Traub-Csekö YM. “Characterization of Lutzomyia Longipalpis Immune Responses with Emphasis in Leishmania Infection” ID 55-O Matthew E Rogers, Emilie Giraud, Rod J Dillon, Paul A Bates, Tamsyn Derrick, Oihane Martin, Ingrid Műller. “Promastigote Secretory Gel from Infected Sand Flies Subverts the Wound Healing Response in an Insulin Growth Factor1-Dependent Manner” ID 56-O Ranadhir Dey, Hamide Aslan, Amritanshu Joshi, Claudio Meneses, Pradeep K. Dagur, John Philip McCoy, Robert Duncan, Jesus G. Valenzuela, Hira L. Nakhasi and Shaden Kamhawi, “VectorTransmission of Leishmania donovani Triggers a Distinct Immune Response that Favors Parasite Survival and Dissemination” ID 57-O Wafa Rebai-Kammoun, Ikbel Naouar, Darragh Duffy, Valentina Libri, Milena Hasan, Amine Toumi, Afif B. Salah, Matthew Albert, Hechmi Louzir, Amel Meddeb-Garnaoui. “Cytokine Levels in Response to Salivary Gland Extracts and to Leishmania major Parasites in Individuals Living in Endemic Areas for Zoonotic Cutaneous Leishmaniasis in Tunisia” ID 58-O Ikbel Naouar, Amel Meddeb-Garnaoui, Amine Toumi, Nabil Belhadj Hamida, Afif B Salah, Hechmi Louzir. Presenting author at the event: Wafa Rebai Kammoun. “Prospective Study of a Cohort of Individuals at Risk of Zoonotic Cutaneous Leishmaniasis in Tunisia to Evaluate Clinical and Immunological Parameters and Identify Correlates of Protection.” ID 59-O Tatiana Di-Blasi; Antonio Jorge Tempone; Erich Loza Telleria; Fabricio K Marchini; José Marcelo Ramalho-Ortigão; Yara Maria Traub-Csekö. “The Possible Role of a Flagellar Protein from Leishmania sp. (FLAG) in the Parasite - Sand Fly Interaction” ID 60-O Votypka J, Pruzinova K, Hlavacova J, Volf P. “The Effect of Temperature and Avian Blood on Leishmania Development in Sand Flies”

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ID 66-O Noteila M Khalid, Marium A Aboud, Fathi M Alrabba, DiaEldin A Elnaiem and Frederic Tripet. ”Evidence for Genetic Differentiation at the Microgeographic Scale in Phlebotomus papatasi Populations from Sudan” ID 67-O Hector M Diaz-Albiter, Caroline d Moraes, Maiara d Faria, Mauricio R Sant'Anna, Roderick J Dillon and Fernando A. Genta. “Gene Expression Profiling and Silencing of Key Digestive Enzymes in Lutzomyia Longipalpis” ID 68-O Remoli ME, Bongiorno G, Fortuna C, Marchi A, Bianchi R2, Khoury C, Ciufolini MG, Gramiccia M. ” Evaluation of Collection Methods for Phlebotomus-Borne Viruses Detection: Isolation and Viral RNA Integrity Performance” ID 69-O Andrea Martins da Silva, Antonio Jorge Tempone, André N Pitaluga, Fabricio K Marchini, Barbara Burleigh, Yara M TraubCseko. “Conditioned Media and Exosomes from Lutzomyia longipalpis LL5 Cells dsRNA-Transfected are able to Induce NonSpecific Antiviral Response in LL5 Naive Cells” ID 70-O Meneses C, Morales E, Rhodes C, Oliveira F, Valenzuela JG and Kamhawi S. “CD-Rom Sand Fly Fellas: The First Complete Video-Based Sand Fly Rearing Guide in High Definition” ID 71-O Vicente Estevam Machado; Thais Marchi Goulart; Arlene Gonçalves Corrêa; Mara Cristina Pinto. “Comparison of Volatile Compounds Emitted by Animals or Present in Plants on Attractiveness of Nyssomyia neivai (Diptera:Psychodidae) ID 72-O Ifhem Chlebi, Elyes Zhioua, and Gordon Hamilton. “Responses of the Sand Fly Phlebotomus papatasi Scopoli (Diptera: Psychodidae) to an Oviposition Pheromone Associated with Conspecific Eggs” ID 73-O Vivero J Rafael; Cadavid Gloria; Moreno H Claudia Ximena; Posada-López Laura; Sandra Uribe S. “Study on the Bacterial Midgut Microbiota Associated to Immature Stage of Genus Lutzomyia (Diptera: Psychodidae) Isolated from Natural Breeding Sites”

ID 61-O Mary Ann McDowell and the Sand Fly Genome Consortium. “Two Sand Fly Genomes: Phlebotomus papatasi And Lutzomyia longipalpis”

POSTERS

ID 62-O Gloria I Giraldo-Calderón, Scott J. Emrich, Daniel Lawson, Frank H Collins and the members of the VectorBase consortium. “The Database Vectorbase and the Genomes of Lutzomyia longipalpis and Phlebotomus papatasi”

ID 1-P Sirlei Franck Thies, Ana Lucia Maria Ribeiro, Jorge Senatore Rodrigues Vargas, Edelberto Santos Dias and Amilcar Sabino Damazo “Sandfly fauna (Diptera: Psychodidae) of urban area in the city of sinop, Mato grosso, Brazil”

ID 63-O Samuel Tadros, Mariha Wadsworth, Mallory Hawksworth, RJ Nowling, Mary Ann McDowell, Gloria I Giraldo-Calderón. “Manual Annotation of G Protein–Coupled Receptors (GPCRs) in the Genomes of Lutzomyia longipalpis and Phlebotomus papatasi”

ID 2-P Sirlei Franck Thies, Marinês Browers “Sandfly fauna (Diptera: Psychodidae) from regional of health in Sinop, Mato Grosso, Brasil, year 2013.”

ID 64-O Gabriela Vela, Gabriel Trueba, Jérôme Depaquit, Sonia Zapata. “Genetic Variation of Nyssomyia trapidoi (Diptera: Psychodidae) in Three Endemic Zones of Leishmaniasis in the Ecuador”

ID 3-P Lorena G. Caligiuri, Enrique A. Sandoval, María Soledad Santini, Soraya Acardi, Oscar D. Salomón, Lilian Tartaglino and Christina B. McCarthy “Testing the versatility of a Psychodiella sp. diagnostic assay in field surveys” ID 4-P Inés Martín-Martín, Ricardo Molina and Maribel Jiménez “Kinetics of anti-Phlebotomus perniciosus saliva antibodies in mice and rabbit será”

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Boletín de la Sociedad Entomológica Argentina

ID 5-P Laura W. Juan, Ignacio T. Gould, Oscar D. Salomón, Juan Molina, Raul A. Alzogaray and Eduardo N. Zerba “Field evaluation of an insecticide formulated with active ingredients acting on larval and adult stages of Lutzomyia longipalpis”

Visceral Leishmaniasis in northeast of Brazil: Fortaleza, state of Ceará, Brazil”

ID 6-P Douglas De Almeida Rocha, Thaís Oliveira Coelho, Rodrigo Gurgel Gonçalves, Andrey José de Andrade and Marcos Takashi Obara “Characterization of susceptibility of populations of Lutzomyia longipalpis (Diptera: Psychodidae) to alphacipermetrin” ID 7-P Tobin Rowland, Silas Davidson, Kevin Kobylinski and Edgar Rowton “Effects of Leishmania major Infection on Sand Fly Feeding Responses to DEET and or permethrin treated uniform material” ID 8-P Horacio Cadena, Edgar Ortega, Luz A. Acosta, Andrés Vélez-Mira, Rafael J. Vivero,Eduar E. Bejarano, Sandra Uribe, Hernando Gómez, Luis G. Estrada and Iván D. Vélez “Soil analysis and vegetation associated with the breeding sites of Lutzomyia evansi in the municipality of Ovejas-Sucre a focus of leishmaniasis in Colombia”

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ID 17-P Fernandes Brilhante A., Galati E. A. B, de Oliveira Cardoso C., Progênio dos Santos N., Ferreira de Souza J. and Vidal J. “Phlebotominae captured in area of transmission of American cutaneous leishmaniasis (ACL) in Xapuri municipality, Acre state, Western Brazilian Amazonian. Preliminary results” ID 18-P dos Santos Afonso M. M., de Miranda Chaves S. A., de Avelar Figueiredo Mafra Magalhães M., Garrido R., Graser C., de Carvalho B. M., Costa S. M. and Rangel E. F. “The geographic expansion of American Visceral Leishmaniasis associated with deforestation in Tocantins State, Brazil” ID 19-P dos Santos Afonso M. M., de Miranda Chaves S. A., de Pita Pereira D., da Costa Rego T. A., de Santana A. L. F., dos Santos Silva J., de Carvalho B. M., da Costa S. M., Giordano C. and Rangel E. F.“Lutzomyia (Lutzomyia) longipalpis feeding habits and natural infection by Leishmania (Leishmania) infantum chagasi in some Brazilian endemic areas of American visceral leishmaniasis”

ID 9-P David Poché, Alex Gendernalik, Larisa Polyakova, Richard Poché, Madina Kozhahmetova, Batirbek Aimakhanov, Ziyat Abdeliyev and Bakhit Atshabar “The use of systemic insecticides to control Phlebotomus spp. sand flies in southern Kazakhstan”

ID 20-P Stramandinoli Moreno E., Posteraro Freire M., Cordeiro Nascimento L. O., Zanini V., Ribeiro Alves V. , Fernandes Shimabukuro P. H. “Sand flies (Diptera, Psychodidae, Phlebotominae) collected in the Wajãpi Indigenous Reserve, Eastern Amazon, Brazil”

ID 10-P José Carlos C B Ribeiro, Antônio Menezes Filho, Daniela M Parente, Teresinha C Farias, Danielle A Zacarias, Jalthon C da Silva, Dorcas L. Costa, Ivete L Mendonça, Carlos H N Cost “Transmission of Leishmania infantum to Lutzomyia longipalpis by humans with and without HIV infection, symptomatic and asymptomatic”

ID 21-P Fernández M. S., Santini M. S., Cavia R. and Salomón O. D. “Lutzomyia longipalpis (Leishmania infantum vector) and Nyssomyia whitmani (Leishmania braziliensis vector) in the city of Puerto Iguazú, northeastern Argentina: seasonal abundance and relationship with domestic animals”

ID 11-P Quintana MG., Santini MS, Britez N, Rojas de Arias A, Thomaz-Soccol V, Bisetto A, Bazzani R, Yadon Z, Salomón OD and the IDRC Project #107577 team “Addressing the emergence and spread of leishmaniasis in the borders of Argentina, Brazil and Paraguay”

ID 22-P Machado C. A., Ribeiro Alves V. and Fernandes Shimabukuro P. H. “Geographical distribution of Nyssomyia Barretto, 1962 in Brazil” ID 23-P Rosa J. R., Parras M. A., Szelag E. A and Salomón O. D. “Vertical and horizontal distribution of sand flies in an area of Tegumentary Leishmaniasis in Chaco, Argentina”

ID 12-P Fitipaldi Veloso Guimarães V. C., Lemos Costa P., da Silva F. J., Lopes de Melo F., Dantas-Torres F., Gomes Rodrigues E. E. and Pinto Brandão-Filho S. “Molecular detection of Leishmania in phlebotomine sand flies in a cutaneous and visceral leishmaniasis endemic area in northeastern Brazil”

ID 24-P Parras M. A., Rosa J. R., Szelag E. A. and Salomón O. D. “Natural breeding sites of Phlebotominae (Diptera: Psychodidae) in a flood zone of Chaco, Argentina”

ID 13-P Menezes, J.A., Sousa A. M., Morais M. H. G., Andrade-Filho J. D., Lima F. P., Fux B. and Margonari C. “Study of sand fly fauna in recent area transmission of leishmaniasis in the state of Minas Gerais, Brazil”

ID 25-P Carvalho G.M.L., Quaresma P. F., Gontijo C. M. F., Rego F. D., Rugani J. M. N., Dias T. A. and Andrade Filho J. D. “Analysis of sand fly (Diptera: Psychodidae) bloodmeals using sequencing technique: test of sand fly experimentally fed”

ID 14-P de Andrade A. J. and Gurgel-Gonçalves R. “New record and update on the geographical distribution of Pintomyia monticola (Costa Lima, 1932) (Diptera: Psychodidae: Phlebotominae) in South America” ID 15-P Rocha Dos Santos C. F., Lima M. C., Da Rocha Neto G. B. and Moreira Macena A. L. “Entomological monitoring of the programme of visceral leishmaniasis in Maceió, Alagoas State, Brazil: indicators and environmental risk factors”

ID 26-P Acel A. M., Ribeiro A. L. M., Miyazaki R. D. and Senatore Vargas Rodrigues J. “Sandfly survey in ecotourism region in the district of Bom Jardim from the City of Nobres, Mato Grosso – Brazil” ID 27-P Ribeiro A. L. M., Senatore Vargas Rodrigues J., Parreiras de Almeida T., Marques de Souza E., Pereira Barreto Bettiol F. K., Acel A. M, Leal dos Santos F. A., Franck Thies S. and Dunjko Miyazaki R. “Leishmaniasis sandfly diversity in a rural area of the Cuiabá, Mato Grosso, Brazil”

ID 16-P Silva R. A., Bevilaqua C. M. L., Rangel E. F., Santos F. K.M., Sousa L. C., Donato L. E. and Pessoa G. C. D. “Aspects of the ecology of Lutzomyia longipalpis and Lutzomyia migonei (Diptera: Psychodidae: Phlebotominae) in area of intense transmission of

ID 28-P Dunjko Miyazaki R., Ribeiro A. L. M., Senatore Vargas Rodrigues J., Parreiras de Almeida T., Marques de Souza E., Pereira Barreto Bettiol F. K. and Acel A. M. “Incidence of sandflies at the zoological of Federal University of Mato Grosso, Cuiabá/MT-Brazil”

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ID 29-P Lemma W., Tekie H., Balkew M., Gebre-Michael, Warburg A. and Hailu A. “Population dynamics and habitat preferences of Phlebotomus orientalis in extra-domestic habitats of Metema Humera lowlands - kala-azar endemic areas in northwest Ethiopia”

ID 42-P da Costa S. M., Cordeiro J. L. P, Afonso M.S.11 and Rangel E. F. “Habitat Suitability for Lutzomyia (Nyssomyia) whitmani and American Cutaneous Leishmaniasis (ACL) in Brazil”

ID 30-P Lemos Costa P., Dantas-Torres F., José da Silva F., Fitipaldi Veloso Guimarães V. C., Gaudêncio K. and Pinto Brandão Filho S. “Ecology of Lutzomyia longipalpis in an area of visceral leishmaniasis transmission in north-eastern Brazil” ID 31-P de Oliveira Miranda D. E., Gaudêncio da Silva K., da Gloria Faustino M. A., Câmara Alves L., Pinto Brandão-Filho S., DantasTorres F. and de Carvalho G. A. “Diversity of phlebotomine sand flies in an american cutaneous leishamaniasis endemic area in northeastern brazil” ID 32-P Gaudêncio da Silva K., Silva de Morais R. C., de Oliveira Miranda D. E., de Arruda Moura A. C., Pinto Brandão-Filho S., de Paiva Cavalcanti M. and Dantas-Torres F. “A real-time PCR for characterization of multiple blood sources in field-collected phlebotomine sand flies” ID 33-P Maia C., Cristóvão J., Freitas F. B., Afonso M.O. and Campino L. “A three consecutive years phlebotomine sand fly survey in a zoonotic leishmaniasis endemic focus, the Algarve Region, Portugal” ID 34-PLemma W, Tekie H, Abassi I, Meshesha Balkew M., GebreMichael T., Warburg A. and Hailu A. “Nocturnal Activities and Host Preferences of P. orientalis in Extra-Domestic Habitats of Kala-Azar Endemic Metema–Humera Lowlands, North West Ethiopia” ID 35-P Rigg C., Valderrama A, Calzada J.E., Chavez L. F. and Saldaña A. “Prevalence of Leishmania spp. infection and host - blood feeding of sandflies transmitters of cutaneous leishmaniasis in an endemic locality in the district of capira, Panama” ID 36-P Arserim S.K., Karakus M. and Ozbel Y. “A study on nocturnal activity of sand flies in a leishmaniasis endemic village located in Aydin province of Turkey” ID 37-P Arserim S. K., Mermer A., Balcioglu I.C., Ermis V. O. and Ozbel Y. “Seasonal Activities and Ecological Features of Sand Flies (Diptera: Psychodidae: Phlebotominae) in Aydın Mountains and Surroundings Located in Western Turkey, Endemic Area for Cutaneous Leishmaniasis” ID 38-P Araujo A. V., Benítez Ibalo A. P., Rosa J. R., Oscherov E. B. and Salomón O. D. “Bionomy of Phlebotomine sandflies (Phlebotominae) in an outbreak area of visceral leishmaniasis in Corrientes, Argentina”

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ID 43-P Gould, I. T., Fernández M. S., Pérez A A. and Salomón O. D. “A field study to evaluate the performance of two modified CDC light traps for collecting sandflies compared with the traditional CDC trap light” ID 44-P Gómez Bravo A., Gould I. T., Abril M., Salomón O. D. “Population composition and dynamics of Lutzomyia longipalpis and other Phlebotominae species in Clorinda, Northern Argentina” ID 45-P Acardi S. A, Steinhorst I. I., Santini M. S., Sandoval A. E., Salomon O. D. “Description of population dynamics and hourly activity of Lutzomyia longipalpis in the city of Posadas, Argentina” ID 46-P Mondragon-Shem K., Bell M., Suar M., Routray P., Posada L. and Cameron M “Comparison of CDC light and gravid traps for the collection of sandfly fauna in Bhubaneswar, Orissa (India)” ID 47-P Ilango Kandan “Taxonomy and diagnostic characters of the Phlebotomus argentipes species complex” ID 48-P Rodrigo E. Godoy, Eunice A. B. Galati, Nataly A. de Souza, Thiago V. dos Santos, Lindemberg Caranha de Sousa, Adriana Zwetsch and Elizabeth F. Rangel “Discriminant analysis as a possible tool to identification Sand flies (Diptera: Psychodidae) species through morphometric data” ID 49-P Jomaa Chemkhi, Souheila Guerbouj, Ikram Guizani and Afif Ben Salah “First report of abnormal numbers of spermathecae in a female Phlebotomus (Larroussius) longicuspis (Diptera: Psychodidae) in Tunisia” ID 50-P D. de Almeida Rocha, J. Barbosa C. Ferreira, R. GurgelGonçalves and Andrey J. de Andrade “First record of Psathyromyia pradobarrientosi (Le Pont, Matias, Martinez & Dujardin, 2004) (Diptera: Psychodidae: Phlebotominae) in Brazil” ID 51-P Priscila B. Sábio, Andrey J. de Andrade and Eunice A. B. Galati “Records of females Psathyromyia cuzquena Martins, Llanos & Silva, 1975 (Diptera, Psychodidae, Phlebotominae) in northern Brazil” ID 52-P Priscila B. Sábio, Andrey J. de Andrade and Eunice A. B. Galati “Assessment of the taxonomic status of Phlebotomus pifanoi Ortiz, 1972 (Diptera, Psychodidae, Phlebotomina ID 53-P Nil Rahola " Uncharted Phlebotomine sand flies in Gabon”

ID 39-P Kirstein O. D., Faiman R., Gebreselassie A., Hailu A, GebreMichael T. and Warburg A. “Attraction of Ethiopian phlebotomine sand flies (diptera: psychodidae) to light and sugar-yeast mixtures (Co2)”

ID 54-P Patrick F. Botelho, Fábio M. Medeiros da Silva, Rodrigo E. Godoy and Thiago V. dos Santos “Morphometric study on populations of Psychodopygus complexa and Ps. Wellcomei (Diptera: Psychodidae), vectors of cutaneous leishmaniasis in Brazil”

ID 40-P Moreira de Carvalho B., Moncassim Vale M., Lorini M. L., dos Santos Afonso M. M. and Rangel E. F. “Ecological niche modeling predicts southward expansion of Lutzomyia flaviscutellata, vector of diffuse cutaneous leishmaniasis in South America, under climate change”

ID 55-P Veracilda R. Alves, Claudio Ruy V. da Fonseca and Paloma H. F. Shimabukuro “Taxonomic review of Psychodopygus nangabeira, 1941 (Diptera, Psychodidae, Phlebotominae

ID 41-P Kirstein o. D., Abassi I., Gebreselassie A., Gebre-Michael T., Hailu A. and Warburg A. “Identification of Plants fed upon by phlebotomine sand flies in Ethiopia”

ID 56-P Priscila B. Sábio, Andrey J. de Andrade and Eunice A. B. Galati “Taxonomic revision of the subgenus Psathyromyia Barretto, 1962 (Diptera: Psychodidae: Phlebotominae)” ID 57-P Maia C., Parreira R., Cristóvão J., Afonso M.O. and Campino

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L. “DNA Barcoding for the Identification of Sand Fly Species (Diptera, Psychodidae, Phlebotominae) in Portugal”

longipalpis in henhouses in an American visceral leishmaniasis endemic area of São Paulo State”

ID 58-P Casertano S.A., Andrade Filho J.D. and Salomon O.D. “New records of Phlebotominae (Diptera: Psychodidae) from the Atlantic Forest region of Argentina”

ID 72-P Ferreira Souza C., Quaresma P. F., Dilermando Andrade Filho J., Peçanha Brazil R. and Dias Bevilacqua P. “Micropygomyia quinquefer (Dyar, 1929) (Diptera: Psychodidae: Phlebotominae): a puctative vector of Leishmania spp. ?”

ID 59-P Pérez-Doria A., Romero-Ricardo L., Lastre N. and Bejarano Eduar E. “A method for extraction of genetic material which permits the integration of classical taxonomy with identification based on the DNA barcode” ID 60-P Cristian F. Souza, Reginaldo P. Brazil, José D. Andrade Filho, Paula D. Bevilacqua, Patrícia F. Quaresma and Mirelle B. Souza “Contribution to the knowledge of the fauna of sand flies (Diptera: Psychodidae: Phlebotominae) of Rio Doce State Park, Minas Gerais, Brazil” ID 61-P Amini Esfanjani MH, Barazesh A, Mazloumi Gavagni A and Saeidi Z “Sand fly fauna in Kaleybar districts, northwestern Iran” ID 62-P Mierez M. L., Rea M. J. F., Borda C. E. and Mosqueda L. A. “Malformations of the genitalia in male Micropygomyia quinquefer (Dyar, 1929) (Diptera: Psychodidae) in Corrientes, Argentina” ID 63-PPosada-López L., Vivero R., Uribe S. I., Acosta L. A., VélezMira A., Cadena H., Estrada L. G. , Ortega E., Bejarano E. and Vélez I. D. “The use of DNA Barcoding for taxonomic identification of the immature stages of phlebotomine sandflies in ovejas, sucre (Colombia)” ID 64-P Fernandez R., Zorrilla V., Espada L., Vásquez G. and Stell F. “Sand flies (Diptera: Psychodidae) from Peru: report of new records”

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ID 73-P Kratochvílová T., Košťálová T., Kindlová M., Sumová P., Šíma M., Rohoušová I., Gramiccia M., Gradoni L., Hailu A., Baneth G., Warburg A. and Volf P.“Canine antibody response to Phlebotomus perniciosus and Phlebotomus orientalis bites” ID 74-P de Oliveira Lara-Silva F., Monteiro Michalsky E., Latorre Fortes-Dias C., de Oliveira Pires Fiuza V., Marques Pessanha J.E., da Silva S. R., Moreira de Avelar D., Alves Silva M., Vianna Mariano da Rocha Lima A.C., da Costa A. J. A., Machado-Coelho G. L. L. and Edelberto Santos Dias E. “Epidemiological aspects of vector, parasite, and domestic reservoir in area with no reported human cases of visceral leishmaniasis in Brazil” ID 75-P Monteiro Michalsky E., Valadão Lopes J., Oliveira Lara E Silva F., Alves SilvaM., Vianna Mariano Da Rocha Lima A. C., França-Silva J. C., Rodrigues Da Paixão F., Antunes Da Costa A. J. and Santos Dias E. “Sandflies fauna survey and DNA detection of Leishmania infantum in Lutzomyia longipalpis captured in the city of Sabara, Minas Gerais, Brazil” ID 76-Pde Nazaré Martins da Silva A., Ishikawa E. A. Y., Fadul M., Vasconcelos dos Santos T., Costa Vasconcelos dos Santos Y., Carvalho de Araújo P. T. and Queiroz Santos N. “Phlebotomine sand flies (Diptera: Psychodidae) in three municipalities of the state of Pará, Brazil, area of very high transmission of cutaneous leishmaniasis”

ID 65-P Fuenzalida AD, Direni Mancini JM, Salomon OD, and Quintana MG “First record of the genus Pintomyia Costa Lima, 1932 (Diptera Phlebotominae) in the northwest of Argentina”

ID 77-P Moreira Macena A L., Cavalcante De Matos Rodarte C., Tavares Da Silva M. E., Rocha Dos Santos C. F., Carneiro Lima M. and Braga Da Rocha Neto G. “Preliminary study of sandflies in the city of Maceió-Alagoas-Brazil”

ID 66-P Reis, A. S., Saraiva, L., Gontijo, C.M.F., Andrade Filho, J. D. " Morphometric study of Lutzomyia longipalpis populations (Diptera: Psychodidae) collected in urban and environmental protected areas in Minas Gerais, Brazil”

ID 78-P de Morais Oliveira A. F., Lima Batista H., Azevedo Leite K. C. and Nunes Lacerda H. M. “Education: demographic and social impacts on the rate of incidence of Visceral Leishmaniasis in the State of Tocantins, Brazil (2010)”

ID 67-P Almazán M. C., Gil J. F., Chanampa M. M., Barroso P., Nasser J, Copa G. N., Oreste F., Castillo G. “Sand flies captures and identification of Leishmania subgenus in Giemsa-Stained slides of patients from five localities of Salta, Argentina”

ID 79-P Silva Lana R., Monteiro Michalsky E., França Silva J. C., de Oliveira Lara e Silva F., Latorre Fortes Dias C., Martins J. and Dias E. S. “Eco-epidemiology of leishmaniasis in the region of Serra do Cipo, a major touristic centreof Minas Gerais”

ID 68-P Szelag E. A., Rosa J. R., Parras M. A. and Salomón O. D “Phlebotominae Bionomics in the Humid Chaco Bio-region. Chaco, Argentina”

ID 80-P de Moraes S. C., Lane Melo A. and Rangel E. F. “Survey of sandflies (Diptera: Psychodidade: Phlebotominae) in indoor environments, peridomestic and extra-domestic transmission area of American cutaneous leishmaniasis (ACL) and American visceral leishmaniasis (AVL) in Mato Grosso, Brazil”

ID 69-P Szelag E. A., Parras M. A., Rosa J. R. and Salomón O. D “Dispersion of Lutzomyia longipalpis in the Chaco province, Argentina” ID 70-P Galvis Ovallos F., da Paixão Sevá A., Galucci E., Martins Soares R., Ferreira F. and Galati E. A. B. “Monthly distribution of Lutzomyia longipalpis in an endemic area of visceral leishmaniasis in São Paulo state, Brazil” ID 71-P Galvis Ovallos F., da Paixão Sevá A., Galucci E., Martins Soares R., Ferreira F. and Galati E. A. B. “Frequency of Lutzomyia

ID 81-P de Moraes S. C., dos Santos L. H. M., Nunes Alexandre T. and Brito do Nascimento T. “Sandfly fauna (Diptera: Psychodidae) in endemic area for transmission of leishmaniasis and the relation of the presence of Lutzomyia (Lutzomyia) longipalpis and Lutzomyia (Nyssomyia) whitmani whit information demographics” ID 82-P Torina A., Reale S., Blanda M., La Russa F., Intile S., Lelli R., Vitale F. “Study of the abundance and the distribution of Sandfly in Sicily”

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ID 83-P Berrozpe P., Araujo A., Santini M. S., Utges M. E. and Salomon O.D. “Lutzomyia longipalpis distribution and abundance in the city of Corrientes, northeastern Argentina” ID 84-P de Oliveira Moura Infran J., Alovisi SouzaD., Fernandes de Souza W., Casaril A. E., Utida Eguchi G., Teruya Oshiro E., Eurico Fernandes C. and Gutierrez de Oliveira A.“ Study of Phlebotominae in modified white and black Shannon traps in a Maroon area at Piraputanga district, Aquidauna, Mato Grosso do Sul, Brazil”

ID 95-P Dutra Rego F., Marteleto Nunes Rugani J., Quaresma P. F., Fernandes Shimabukuro P. H., Barbosa Tonelli G., Carvalho Scholte R. G., and Ferreira Gontijo C. M. “Molecular detection of Leishmania in sand flies (Diptera: Psychodidae) and spatial distribution of American Cutaneous Leishmaniasis in the Xakriabá Indigenous Reserve, Brazil”

ID 85-Pde Oliveira Moura Infran J., Alovisi SouzaD., Fernandes de Souza w., Casaril A. E., Utida Eguchi G., Elia Teruya Oshiro E., Fernandes C. E. and Gutierrez de Oliveira A.“Nycthemeral rhythm of Phlebotominae in a Maroon area at Piraputanga district, Aquidauna, Mato Grosso do Sul, Brazil” ID 86-P Bustamante Gomez M., Diaz M., Espinoza J., Parrado R., Reithinger R. and Lineth Garciá A. “Sand Fly Fauna in Chapare, Bolivia: An Endemic Focus of Leishmania (Viannia) braziliensis” ID 87-PEspinoza Echeverria J., Bustamante M., Diaz M., Parrado R. and Lineth Garciá A. “Diversity and nycterohemeral activity of phlebotomines in San Julian community (Cochabamba, Bolivia)” ID 88-P Casaril A. E., Falcão de Oliveira E., Zandonaide Monaco N., Utida Eguchi G., Rezende de Figueiredo H.,Lopes Fontoura Mateus N., de Souza Fernandes W., Petilim Gomes S., Alovisi Souza D., Oliveira Moura Infran J., Teruya Oshiro E., Galati E. A. B. and Gutierrez de Oliveira A. “The Sandfly Fauna (Diptera: Psychodidae: Phlebotominae) in Corumbá, Mato Grosso do Sul, Brazil” ID 89-Pdos Santos Silva J., Pereira dos Santos A., Kássio Moura Santos F., Rodrigues da Silva L. O., Caranha L. and Rangel E. F. “Entomologic surveillance of Phlebotomine sand flies vectors of Leishmaniasis in areas impacted by the Transfer of the São Francisco River project, in the state of Ceará, Brazil” ID 90-P Gebresilassie A., Kirstein O. D., Moncaz A., Tekie H., Balkew M., Warburg A., Hailu A., and Gebre-Michael T. “The Influence of moon light and lunar periodicity on the density of Phlebotomus (Larroussius) orientalis Parrot, 1936 (Diptera: Psychodidae) from light trap collections in Ethiopia” ID 91-PRodríguez-Jiménez J., Pérez-Doria A., Romero-Ricardo L., Vergara J., Montesino A., Martínez L., Cortés L., Cochero S., and Bejarano E. E. “Characteristics of human dwellings and their surroundings that favour the abundance of phlebotomines in four urban areas of the Caribbean coast of Colombia” ID 92-P Barata I. da R., Pinheiro M. S. B., da Silva F. M. M., Feitosa Brandão R. C., dos Santos T. V. and Silveira F. T. “Phlebotomine sand fly fauna (Diptera: Psychodidae) and natural flagellate infections from An area highly endemic for cutaneous leishmaniasis on the Brazilian-Bolivian frontier in the state of Acre, Brazil” ID 93-P Franck Thies S., Ribeiro A. L. M., Senatore Rodrigues Vargas J., Djunko Miyazaki R., Monteiro Michalsky E. and Santos Dias E. “Sandflies (Diptera: Psychodidae) naturally infected by Leishmania in rural areas of the city of Nova Mutum, Mato Grosso, Brasil” ID 94-P da Silva T. R. R., Gobira Guimarães de Assis M. D., Dutra Rêgo F., Posteraro Freire M. and Fernandes Shimabukuro P. H. “Sand fly fauna (Diptera, Psychodidae) collected in the Middle Purus river region, Amazonas, Brazil”

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ID 96-P Jomaa Chemkhi, Rihab Yazidi, Mahdi Driss, Jihene Bettaieb and Afif Ben Salah “First report of natural promastigote infection of Sergentomyia dreyfussi (Diptera: Psychodidae) sand flies in an endemic focus of cutaneous leishmaniasis in central Tunisia” ID 97-P Laurenti M. D., Francesquini F. C., Passero L. F., Tomokane T. Y., Carvalho A. K., Corbett C. E. P. and Silveira F. T. “Salivary gland homogenates from wild-caught Lutzomyia flaviscutellata and L. (Psychodopygus) complexus did not exacerbate Leishmania (L.) amazonensis and L. (V.) braziliensis infection in BALB/c mice” ID 98-P Antonio Marque Pereira Júnior, Carolina Bioni Garcia Teles, Ana Paula de Azevedo dos Santos, Felipe Arley Costa Pessoa, Jansen Fernandes de Medeiros. "Natural infection of phlebotomine sand flies (Diptera: Psychodidae) by Leishmania Ross (Kinetoplastida: Trypanosomatidae) in “terra firme” and “várzea” enviroments in Tefé municipality, Amazonas state, Brazil” ID 99-P Martín-Martín I, Jiménez M., González E. and Molina R. “Transmission of Leishmania infantum to hamsters through the bite of experimentally infected Phlebotomus perniciosus and monitoring of animals infectivity by xenodiagnosis” ID 100-P Bates P.A., Bates M.D. and Kwakye-Nuako G. “Phylogenetic analysis of the Leishmania enriettii complex and speculations on their vector biology” ID 101-P Fernandes M.F., dos Santos K. M., Ferrari Júnior A. C., Silva Levay A. P., Negrão F. J., Fernandes W. D., Oshiro E. T., Cavalheiros Dorval M. E. M, Andrade Filho J. D., Gutierrez de Oliveira A. and Galati Eunice A. B. “Vector competence of Nyssomyia whitmani (Diptera: Psychodidae: Phlebotominae) to Leishmania (Leishmania) amazonensis” ID 102-P Rado D., Pita-Pereira D., Araujo Pereira T., Quispe-Florez MM, Brahim L., Rangel E., Valladares B. and Pacheco R. “Natural Infection of Lutzomyia (Nyssomyia) y. yuilli and L. (Trichophoromyia) octavioi with Leishmania spp. in Aguas Calientes, La Convencion, Cusco, Perú” ID 103-P Quispe A., Quispe-Ricalde M. A., Rado D., Quispe-Florez MM, Soto M., Pérez J.E., Valladares B. and Pacheco R. “Lutzomyia (Nyssomyia) y. yuilli and L. (Trichophoromyia) sp. from Alto Ivochote, La Convención, Cusco, Peru” ID 104-P Cutolo A. A, Ovallos F.G., Oliveira C., Lavitschka, Dantas da Silva M., Molla L. M., dos Santos Nogueira F., Pereira-Chioccola V. L., Menz I. and Galati E. A. B. “Aggregated pattern of Leishmania infantum chagasi transmission from naturally infected dogs to laboratory reared Lutzomyia longipalpis through xenodiagnosis” ID 105-P Pech-May Angélica, Sánchez-García Laura, BerzunzaCruz Miriam, Becker-Fauser Ingeborg, Escobedo-Ortegón Javier, Rebollar-Téllez Eduardo A. " Vectors of Leishmania mexicana in southeaster of Mexico” ID 106-P Boussaa Samia,

Ouanaimi Fouad,

Kahime Koloud,

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

Echchakery Mohamed, Boumezzough Ali and Pesson Bernard “Genetic characterization of northern and southern populations of Phlebotomus sergenti (Diptera: Psychodidae) populations in Morocco”

Pinheiro M. P., da Silva Lopes T. E., de Medeiros Silva M. M. and da Silva Junior J. B. “Popular knowledge of leishmaniasis transmission in a peri-urban area of the municipality of São Gonçalo do Amarante, Brazil”

ID 107-P Noteila M Khalid, Marium A Aboud, Fathi M Alrabba, DiaEldin A Elnaiem and Frederic Tripet. "Evidence for genetic differentiation at the microgeographic scale in Phlebotomus papatasi populations from Sudan”

ID 119-P Alves M. de L., Freire de Melo Ximenes M. de F, Fernandes de Araújo M. F., Gomes Pinheiro M. P., da Silva Lopes T. E., da Silva Junior J. B. and Gurgel Mendes I.“ Educational comic strip booklet as an instrument of scientific dissemination of leishmaniasis in the state of Rio Grande do norte, Brazil”

ID 108-P Oliveira D. M. S., Lima J. A. N., Silva B. J. M., Farias L. H. S., Santos T.V., Silveira F.T and Silva, E. O. “Analysis of carbohydrate epitopes on the surface of midgut epithelium of Lutzomyia (Lutzomyia) longipalpis and Lutzomyia (Nyssomyia) antunesi (Diptera, Psychodidae) from Pará, State Amazonian Brazil” ID 109-P Bussoni Bertollo D. M., de Cassia Hilário Castilho R., Costa Nunes Soares M. M., Kindler Figueiredo J., da Silva Meneghello B. H.; Mitsuyoshi Hiramoto R. and Tolezano J. E. “Comparative analysis between canine visceral leishmaniasis and sandfly presence in the endemic municipalities” ID 110-P Nunes Peres Dias Q., Barbosa de Souza M., Corrêa Novo S. P., Antônio de Medeiros Meira and do Santo Ponte C. “Survey of sandfly vectors in areas surrounding Pedra Branca Massif, Municipality of Rio de Janeiro, Brazil” ID 111-P Ferrolho J., Gomes J., Alves-Pires C, Cristóvão J.M., Maia C., Campino L. and Afonso M.O. “Rotation of the external genitalia in male Phlebotomine sandflies (Diptera, Psychodidae) in laboratory conditions and in captured specimens in Algarve, Portugal” ID 112-P Vasconcelos dos Santos T., Batista Campos M., Aprígio Nunes Lima J., Feitosa Brandão R. C., Medeiros da Silva F. M., Barros Pinheiro M. S., da Silva Santos L. A., De Freitas Leão E., da Rocha Barata I. and Fernando Tobias Silveira “Some field and laboratory observations on the biology of Nyssomyia antunesi (Diptera: Psychodiadae), a suspected vector of Leishmania (Viannia) lindenbergi in Amazonian Brazil” ID 113-P Goulart T. M., Machado V. E. and Pinto M. C. “Attractiveness of different diets for Nyssomyia neivai (Diptera: Psychodidae) larvae” ID 114-P Goulart T. M., Machado V. E. and Pinto M. C. “Effect of Nyssomyia neivai (Diptera: Psychodidae) density on oviposition” ID 115-P Rita Mukhopadhyay, Goutam Mandal, Jose Orta, Srotoswoti Mandal, Claudio Meneses, Hamide Aslan Suau, Eric Beitz, Jesus Valenzuela, Marcelo Ramalho-Ortigao, Shaden Kamhawi " Role of aquaporins in the physiology of Leishmania vector Lutzomyia longipalpis” ID 116-P Dutra Rêgo F., de Souza Pinto I., Machado C. A., Melim Ferreira G. E., Cupollilo E. and Fernandes Shimabukuro P. H. “Leishmaniasis Virtual Laboratory (LVL) - a new tool for the study of American sand flies (Dipetra, Psychodidade, Phlebotominae)” ID 117-P Mohammad S. Al-Khalifa; Ibraheem M. Al-Hazza and M. Y. Lubbad “Immunological studies on the cutaneous Leishmaniasis in Al-Kharj Region, Saudi Arabia” ID 118-P Alves M. de L., Freire de Melo Ximenes M. de F., Gomes

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ID 120-P Gomes Pinheiro M. P., Freire de Melo Ximenes M. de F., de Melo Pereira Pinheiro M. P., de Lima Alves M. “Cordel folk literature as a tool for preventing leishmaniasis in Brazil” ID 121-P Rea M. J. F., C Borda. E. “Survey of American tegumentary leishmaniasis in the northeast of Argentina during 20112014” ID 122-P Lozano A., Basmadjián Y., Vitale E., Satragno D., Canneva B., Verger L., Tort C., Viera A., Romero S., Ríos C., Lagarmilla P. "Canine Visceral Leishmaniasis. Imported cases in Uruguay, 20102014" ID 123-P de Freitas Rocha A. T., Melo Silva S., Araújo Soares M. R., Costa Fortier D., Silva Barbosa D. B., Nery Costa C. H. " Occurrence of Lutzomyia longipalpis and spatial distribution of visceral leishmaniasis in Floriano, Piauí, Brazil: preliminary data” ID 124-P Santos M. F. C., Paranhos Filho A. C., Gamarra R. M., Fernandes W. D.; Brazil R. P. and Oliveira A. G. “Use of Remote Sensing in the analysis of populations of Lutzomyia longipalpis complex” ID 125-P Utgés M. E., Fuenzalida D., Parras M., Gould I. T., Casertano, S. and Salomón O. D. “Geometric morphometry of northeastern populations of Nyssomyia neivai from Argentina” ID 126-P Rado D., Pérez J. E., Vilela M., Quispe D., Quispe A., Aguilar E. G., Rangel E., Valladares B., Pacheco R. “Sandflies (Diptera: Psychodidae) of Aguas Calientes, La Convención, Cusco, Perú” ID 127-P Toccas F., Chacon M., Pérez J. E., Rado D., Soto M., Mendoza J., Luna R., Aguilar E. G., Valladares B. and Pacheco R. “Lutzomyia spp. (Diptera: Psychodidae) in intra, peri and extradomiciliar environments in Yomentoni (right margin), La Convención, Cusco, Perú” ID 128-P Rezende de Figueiredo H., Casaril A. E., Infran J. O. M., Teruya Oshiro E., Rodrigues Mendes A., Andrade Filho J. D., Moraes Ribeiro L., Falcão Oliveira E., da Cunha Santos M. F. and Gutierrez de Oliveira A. “Ecology of Lutzomyia longipalpis (Diptera: Psychodidae) in Aquidauana, endemic area for leishmaniases, Mato Grosso do Sul, Brazil. Preliminary results” ID 129-P Antonio Marques Pereira Júnior, Eric Fabricio Marialva, Moreno de Souza Rodrigues, Felipe Arley Costa Pessoa, Jansen Fernandes de Medeiros. " Studies on fauna of phlebotomine sand flies (Diptera: Psychodidae) at “Terra firme” and “Várzea” environments in Tefé municipality, Amazonas state, Brazil” ID 130-P Jiménez M., González E., Hernández S. and Molina R. “Phlebotomine sand fly survey in the focus of human leishmaniasis in south western Madrid region, Spain: 2012-2013”

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ID 131-P Jiménez M., González E., Hernández S. and Molina R. “Molecular detection of Leishmania infantum and host-feeding preferences in Phlebotomus perniciosus from the focus of leishmaniasis in south western Madrid region, Spain: 20122013”

P. C., de Almeida M. R. “Sandflies (Diptera, Psychodidae, Phlebotominae) in Pitoco cave, an archeological site in northern Mato Grosso do Sul state, Brazil”

ID 132-P Zoghlami Z., Chouihi E., Barhoumi W., Dachraoui K., Massoudi N., Ben Helel K., Habboul Z., Hadhri§ M. H., Limam S., Mhadhbi M., Gharbi M., and Zhioua E. “Interaction between canine and human visceral leishmaniases in a holoendemic focus of Central Tunisia” ID 133-P Nilsa Gonzalez-Britez, Nilda Portillo, Maria Ferreira, Martha Torales, Nidia Martínez, Luciano Franco. "Leishmaniasis vectors in the Alto Paraná Department, Paraguay” ID 134-P da Silva D. F., de Castilho Sanguinette C., Barbosa Tonelli G., Tanure A., Vidal Stumpp R. G. A, Dutra Rêgo F., Gontijo C. M. F. and do Andrade Filho J. D. “Sand flies (Diptera: Psychodidae: Phlebotominae) collected in urban and wild area in the district of Barra do Guaicui, Várzea da Palma municipality, Minas Gerais, Brazil”

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ID 144-P Santos Carvalho S. M., Fitipaldi Veloso Guimarães V. C., dos Santos P. R. B. “First report of Lutzomyia longipalpis in Uruçuca, cocoa zone of the southern state Bahia, Brazil” ID 145-P Ballart C., González E., Ravel C., Jiménez M., Abras A., Molina R., Portús M., Gállego M. “Identification of blood meals in Leishmania infantum vectors from the Northeast of Spain: Phlebotomus ariasi and P. perniciosus by Polymerase Chain Reaction based and sequencing methods” ID 146-P de Lima Alves M., de Melo Ximenes M. F. F., Pinheiro M. P. G., da Silva J. H. T., da Silva Lopes T. E., de Medeiros Silva M. M. “Phlebotomine sanflies (Diptera: Psychodidae) in a peri-urban setting in the metropolitan region of Natal, Brazil” ID 147-P Toccas F., Rado D., Quispe A., Quispe C., Galindo B., Chacon M., Quispe-Ricalde MA, Valladares B., Pacheco R. “The Sand Fly Fauna (Diptera: Psychodidae) in Alto Ivochote, La Conveción, Cusco, Perú”

ID 135-P Neves Vianna E., Franco Morais M. H., Sobral de Almeida A., Chagastelles Sabroza P., Carneiro M. and Santos Dias E. “Evaluation of the abundance of phlebotomine sand flies in households from an area of Visceral Leishmaniasis transmission in Belo Horizonte, Minas Gerais, Brazil”

ID 148-P Martínez M. F., Santini M. S., Utgés M. E., Kowalewski M., Salomón O. D. “Study of phlebotomines in two wild areas of northeastern Argentina and the potential association with howler monkeys (Alouatta caraya)”

ID 136-P Cavalcante Lamy Serra e Meira P., Lacerda Abreu B., Lusardo de Almeida Zenóbio A. P., de Castilho Sanguinette C., Mayr de Lima Carvalho G., Saraiva L. and Andrade Filho J. D. “Phlebotominae sand fly fauna in two urban parks in Belo Horizonte, Minas Gerais, Brazil (Diptera: Psychodidae)”

ID 149-P de Melo Ximenes M. F. F., Antonaci Gama R., da Silva Lopes T. E., Melo Freire R. C., de Lima Alves M., Pinheiro M. P. G., da Silva J. H. T., de Medeiros Silva M. M. “Phlebotominae sanflies (Diptera: Psychodidae) in a preserved area of the Caatinga biome in Rio Grande do Norte State, Brazil”

ID 137-P Cavalcante Lamy Serra e Meira P. and Andrade Filho J. D. “Species of phlebotomine sand flies (Diptera: Psychodidae: Phlebotominae) reported in Brazilian caves”

ID 150-P Basmadjián Y., Canneva B., Verger L., Vitale E., Lozano A., Sequeira C., Lozano A., Satragno D., Tort C., Viera A., Ríos C., Lagarmilla P. and Romero S. “Lutzomyia longipalpis in Bella Unión, Department of Artigas, Uruguay. One year of surveillance: July 2013- June 2014” ID 151-P Oliveira A.F.J., Freitas R.A., Pessoa F.A.C., Aguiar N.O. “Phlebotomine Sand Fly survey in Central Amazon, Amazonas State, Brazil with a new register of Lutzomyia caligata”

ID 138-P Barbosa Tonelli G., Dutra Rego F., Mayr de Lima Carvalho G., Tanure A. and Andrade Filho J. D. “Ecology of sand flies (Diptera: Psychodidae: Phlebotominae) in the Natural Heritage Private Reserve of Serra do Caraça, Minas Gerais, Brazil” ID 139-P Tanure A., Barbosa Tonelli G., Dutra Rêgo F., Mayr de Lima Carvalho G., Gontijo C. M. F., Fontes Paz G. and Andrade Filho J. D. “Study of sand flies (Diptera: Psychodidae: Phlebotominae) in a transmission area of leishmaniasis in Minas Gerais, Brazil” ID 140-P Souza G. D., A. Cardoso, de Carvalho R. M. J. S. “Assessment of sand flies (Diptera: Psychodidae) in an area with canine visceral leishmaniasis in the municipality of Porto Alegre, Rio Grande do Sul, Brazil” ID 141-P Basmadjián Y., Canneva B., Verger L., Lozano A., Satragno D., Supparo E., Sequeira C., Romero S., Viera A., Tort C., Ríos C., Lagarmilla P. and Vitale E. “Persistence of Lutzomyia longipalpis, vector of Visceral Leishmaniasis, in the city of Salto, Uruguay” ID 142-P Verger L, Lozano A, Vitale E, Satragno D, Sequeira C, Canneva B, Basmadjián Y, Rios C, Lagarmilla P, Tort C, Viera A, Romero S. “Serological surveillance in dogs of the city of Bella Unión, a risk area for Visceral Leishamaniasis” ID 143-P Galati E. A. B., de Almeida P. S., de Paula M. B., Costa Filho

ID 152-P de Arruda Moura A. C., da Silva K. G., D. de Oliveira Miranda E., Ramalho M., da Silva F. J., Brandão-Filho S. P., Otranto D., Dantas-Torres F. “Phlebotominae sanflies in military training area in north-eastern Brazil” ID 153-P Ferro Cr., Lopez M., Fuya P., Lugo L., Cordovez J. M., Gonzalez C. “Relating vector species potential distribution and richness to transmission foci of Cutaneous Leishmaniasis in Colombia” ID 154-P Ballart C., Pesson B., Martín-Sánchez J., Alves-Pires C., Morillas F., Afonso O., Portús M., Gállego M. “Risk of leishmaniosis transmission in the Pyrenean region of Spain assessed through the isoenzymatic analysis of potential sand fly vectors (Phlebotomus ariasi and P. perniciosus)” ID 155-P Barhoumi W., Qualls W. A., Archer R., Fuller D. O., Chelbi I., Cherni S., Derbali M., Arheart K. L., Zhioua E.and Beier J. C. “Irrigation in the arid regions of Tunisia impacts the abundance and apparent density of sand fly vectors of Leishmania infantum”

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ID 156-P Pinheiro M. P. G., de Melo Ximenes M. F. F., de Lima Alves M., da Silva Júnior J. B., da Silva J. H. T., M de Medeiros Silva. M., da Silva Lopes T. E. “ Sandflies (Diptera: Psychodidae: Phlebotominae) from the Nisia Floresta National forest, metropolitan region Natal, Rio Grande do Norte State, Brazil”

ID 170-P Erin Dilger, Orin Courtenay and Graham Medley “Density dependence in host-vector relationships and consequences for transmission”

ID 157-P Erisoz Kasap O., Alten B. “Entomological Survey of Phlebotomine Sand Flies (Diptera: Psychodidae) in Turkey” ID 158-P Copa G. N., Marco J. D., Nasser J. R., Gil J. F. “Distribution of sandflies species in a locality endemic for American tegumentary leishmaniasis from the north of Argentina, bordering with Bolivia” ID 159-P Ruiz-Lopez F., Cadena H., Vélez Mira A., Posada-López L., Contreras A., Tirado Duarte D., Vélez I. D. “Effectiveness of a rapid multidisciplinary protocol for the epidemiological evaluation of an active focus of cutaneous leishmaniasis: Remedios, Antioquia, Colombia” ID 160-P Villarquide M. L., Fernández M. S., Santini M. S., Salomón O. D. “Abundance Association of Nyssomyia whitmani with the number of farm animals and its dwelling distance to the house, in areas with Tegumentary Leishmaniasis transmission in Puerto Iguazú, Misiones, Argentina” ID 161-P Ferro C., Fuya P., Lugo L., González C. “Biodiversity of Phlebotominae subfamily (Diptera: Psychodidae) in the Colombian Amazon and Orinoco” ID 162-P Enriquez S., Ariviliaga J., Vaca F., Benitez Ortiz W., Kirstein O., Abassi I., M Calvopiña., Pozo K., Romero V., O Chiluisa., E Villacrés and Warburg A. “Sand fly vectors of cutaneous leishmaniasis on the Western slopes of the Ecuadorian Andes” ID 163-P Direni Mancini J. M., Fuenzalida A. D., Saracho Bottero M. N., Lizarralde de Grosso M. S., Salomón O. D., Quintana M. G. “Abundance of Phlebotominae in endemic area of cutaneous leishmaniasis, Tucumán, Argentina” ID 164-P Ouanaimi F., Boussaa S., Kahime K., Echchakery M., Boumezzough A. “Phlebotomine sandflies (Diptera: Psychodidae) of Morocco: Results of entomological surveys along the OuarzazatM'hamid, Zagora-Chefchaouen-Nador and Zagora-Tata-Marrakesh roads” ID 165-P Kahime K., Boussaa S., Ouanaimi F. and Boumezzough A. “Spatial relationship between environmental factors and sand fly (Diptera: Psychodidae) distribution associated with leishmaniasis risk in centre & southern Morocco” ID 166-P Boussaa S., Ouanaimi F., Boumezzough A. “Identification and characterization of larval lodging of phlebotomine sand flies (Diptera: Psychodidae) in Marrakech, Morocco” ID 167-P Boumezzough A., Kahime K., Boussaa S. and Ouanaimi F. “Leishmaniasis in Morocco: still a topical question” ID 168-P Remondegui C. V., Cabrera C. H. A., Quintana M. G. “Seasonal fluctuation of Phlebotominae (Diptera: Psychodidae) in three Departments of an endemic area of Cutaneous Leishmaniasis in Jujuy province. A preliminary report” ID 169-P Bisetto Júnior A., Bisetto M., Dotto Ampessam S. M. “Spatial analyisis of the accurrence of leishmaniasis in Paraná, Brazil, to identify areas of risk”

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ID 171-P Casanova C., Fernandes Shimabukuro P. H., Almeida Bressiani B., Fernanda Lazari T., Galati E. A. B. “Geographical distribution of Nyssomyia neivai (Pinto) and Nyssomyia intermedia (Lutz & Neiva) in the state of São Paulo, Brazil” ID 172-P Bergo E., Nascimento L., Camargo-Neves V. “First meeting of Lutzomyia longipalpis in rural areas related to outbreak of canine visceral leishmaniasis in the northwestern region of São Paulo, Brazil” ID 173-P Colla-Jacques F. E., Casanova C., Galvis Ovallos F. “Study of sand fly community in two rural endemic areas for American cutaneous leishmaniasis in the municipalities of Mogi Guaçu and Mogi Mirim, São Paulo State, Brazil” ID 174-P Saraiva L, Rugani J. M. N., Lima A.C.V. M. R., Reis A.S., Pereira A. A. S., Gontijo C.M.F., Andrade Filho J.D. “DNA detection of Leishmania sp. in female sand flies (Diptera: Psychodidae) in Belo Horizonte city and Sumidouro State Park, Minas Gerais – Brazil” ID 175-P Santini M. S., Utges M. E., Casas N., Salomon O. D. “Association Of Occurrence And Abundance Of Lutzomiya longipalpis with Environmental Variables At Different Spatial Scales: Micro And Macro-Scale” ID 176-P Waleed Al Salem, Badriah Al Otaibi, Louise Kelly-Hope, Karina Mondragon-Shem, Salah Balgonaeem, Maha Abdeladhim, Mohammed Al Zahrani, Amir Hassan, Jesus Valenzuela Alvaro Acosta-Serrano “A Public Health Approach to Leishmaniasis Control”

INDEX BY TOPIC Vector Control 1-O, 2-O, 3-O, 4-O, 5-O, 6-O, 7-O, 8-O, 9-O, 10-O, 11-O, 12-O 1-P, 2-P, 3-P, 4-P, 5-P, 6-P, 7-P, 8-P, 9-P, 10-P, 11-P, 176-P Taxonomy & Phylogeny 13-O, 14-O, 15-O, 16-O, 17-O, 18-O, 19-O, 20-O, 21-O, 22-O, 23-O, 24-O 47-P, 48-P, 49-P, 50-P, 51-P, 52-P, 53-P, 54-P, 55-P, 56-P, 57-P, 58-P, 59-P, 60-P, 61-P, 62-P, 63-P, 64-P, 65-P, 66-P Ecology & Epidemiology 25-O, 26-O, 27-O, 28-O, 29-O, 30-O, 31-O, 32-O, 33-O, 34-O, 35-O, 36-O, 37-O, 38-O, 39-O, 40-O, 41-O, 42-O. 43-O, 44-O, 45-O 12-P, 13-P, 14-P, 15-P, 16-P, 17-P, 18-P, 19-P, 20-P, 21-P, 22-P, 23-P, 24-P, 25-P, 26-P, 27-P, 28-P, 29-P, 30-P, 31-P, 32-P, 33-P, 34-P, 35-P, 36-P, 37-P, 38-P, 39-P, 40-P, 41-P, 42-P, 43-P, 44-P, 45-P, 46-P, 67-P, 68-P, 69-P, 70-P, 71-P, 72-P, 73-P, 74-P, 75-P, 76-P, 77-P, 78-P, 79-P, 80-P, 81-P, 82-P, 83-P, 84-P, 85-P, 86-P, 87-P, 88-P, 89-P, 90-P, 91-P, 123-P, 124-P, 125-P, 126-P, 127-P, 128-P, 129-P, 130-P, 131-P, 132-P, 133-P, 134-P, 135-P, 136-P, 137-P, 138-P, 139-P, 140-P, 141-P, 142-P, 143-P, 144-P, 145-P, 146-P, 147-P, 148-P, 149-P, 150-P, 151-P, 152-P, 153-P, 154-P, 155-P, 156-P, 157-P, 158-P, 159-P, 160-P, 161-P, 162-P, 163-P, 164-P, 165-P, 166-P, 167-P, 168-P, 169-P, 170-P, 171-P, 172-P,

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173-P, 174-P, 175-P Vector Competence & Infections 46-O, 47-O, 49-O, 50-O, 51-O, 52-O 92-P, 92-P, 93-P, 94-P, 95-P, 96-P, 97-P, 98-P, 99-P, 100-P, 102-P, 102P, 103-P, 104-P, 105-P Leishmania – Sandfly /host Interactions 48-O, 53-O, 54-O, 55-O, 56-O, 57-O, 58-O, 59-O, 60-O 108-P, 109-P Genome & Genetics/ Virus infections 61-O, 62-O, 63-O, 64-O, 65-O, 66-O, 67-O, 68-O, 69-O 106-P, 107-P, 109-P, 110-P Physiology & Colonization 70-O, 71-O, 72-O, 73-O 111-P, 112-P, 113-P, 114-P, 115-P Other Topics 116-P, 117-P, 118-P, 119-P, 120-P, 121-P, 122-P

INDEX BY AUTHOR (SURNAME) A Abassi Ibrahim 36-O, 34-P, 41-P, 162-P Abdeladhim Maha 8-O, 28-O, 112-O, 176-P Abdeliyev Ziyat 9-P Aboud Marium A 66-O Abras A 145-P Abreu Bruna Lacerda 136-P Abril Marcelo 44-P Acardi Soraya Alejandra 3-P, 45-P Acel Anagela Maria 26-P, 27-P, 28-P Acosta Luz A 26-O, 8-P, 63-P Acosta M Manteca 11-O Acosta-Serrano Alvaro 8-O, 28-O, 176-P Afonso Margarete Martins dos Santos 18-P, 19-P Afonso MO 33-P, 57-P, 111-P, 154-P Aguiar NO 151-P Aguilar E 27-O, 126-P, 127-P Aimakhanov Batirbek 9-P Akhavan AA 40-O Aklilu Esayas 37-O Alexandre Thiago Nunes 81-P Al-Hazza Ibraheem M 117-P Alkan C 39-O Al-Khalifa Mohammad S 117-P Almazán María Cristina 67-P Al Otaibi Badriah 112-O, 176-P Alrabba Fathi M 66-O Al Salem Waleed 28-O, 8-O, 176-P Alten B 21-O, 39-O, 157-P Alves Leucio Câmara 31-P Alves Maria de Lima 118-P, 119-P, 120-P, 146-P, 149-P, 156-P Alves-Pires C 111-P, 154-P Alves Veracilda Ribeiro 20-P, 22-P, 55-P Al Zahrani Mohamed 8-O, 28-O, 176-P

Andrade-Filho José Dilermando 65-O, 13P, 25-P, 58-P, 60-P, 66-P, 72-P, 101-P, 128-P, 134-P, 136-P, 137-P, 139-P, 174-P Alzogaray Raul A 5-P Amini Esfanjani MH 61-P Ampessam Sônia Maria Dotto 169-P Añez Néstor 25-O Arandian MH 40-O Araujo Analia V 38-P, 83-P Archer Reginald 155-P Arcones Carolina 17-O, 22-O Arheart Kristopher L 155-P Arivillaga Jazzmin 162-P Arserim Suha K 4-O, 36-P, 37-P Atshabar Bakhit 9-P Austin James 1-O Aydın S 39-O B Balcioğlu I Cüneyt 37-P, 4-O Balgonaeem Salah 8-O, 176-P Balkew Meshesha 35-O, 36-O, 37-O, 38-O, 29-P, 34-P, 90-P Ballart C 145-P, 154-P Baneth Gad 73-P Banuls A-L 39-O Barata Iorlando da Rocha 92-P, 112-P Barazesh A 61-P Barbosa Diogo Brunno e Silva 123-P Bargues María Dolores 18-O Barhoumi W 3-O, 44-O, 132-P, 155-P Barresi Trey 2-O Barroso Paola A 67-P Basano Fabrizio Solari 12-O Basmadjián Yester 122-P, 141-P, 142-P, 150-P Bates MD 100-P Bates Paul A 50-O, 55-O, 100-P Batista Hebert Lima 78-P

Becker-Fauser Ingeborg 105-P Beier John C 155-P Beitz Eric 115-P Bejarano Eduar Elías 26-O, 46-O, 51-O, 8P, 59-P, 63-P, 91-P Belen A 39-O Bell Melissa 46-P Ben Helel K 132-P Ben Salah Afif 49-P, 96-P Bénié Goze Bertin 32-O Benítez Ibalo P 38-P Bergo Eduardo 172-P Berrozpe Pablo 83-P Bertollo Denise Maria Bussoni 109-P Berzuna-Cruz Miriam 105-P Bettaieb Jihene 32-O, 96-P Bettiol Flávia Karolina Pereira Barreto 27P, 28-P Bevilaqua Claudia ML 16-P Bevilacqua Paula Dias 72-P, 60-P Bianchi R 68-O Bichaud L 44-O Bisetto Júnior Alceu 11-P, 169-P Bisetto Maurício 169-P Blanda M 82-P Bongiorrno G 45-O, 68-O Borda Carlos Edgardo 62-P, 121-P, Borges-Alves G 14-O Botelho Patrick Feitosa 54-P Boujaâma A 3-O Boumezzough Ali 106-P, 164-P, 165-P, 166-P, 167-P Boussaa Samia 106-P, 164-P, 165-P, 166-P, 167-P Brahim L 102-P Brandão-Filho Sinval Pinto 12-P, 30-P, 31P, 32-P, 152-P Brandão Roberto Carlos Feitosa 92-P, 112P

N° 26 (1) 2015 Bray DP 14-O Brazil Reginaldo Peçanha 14-O, 60-P, 72P, 124-P Bressiani Bruna Almeida 171-P Brianti Emanuele 12-O Brilhante Andreia Fernandes 17-P Browers Marinês 2-P Burleigh Barbara 69-O Burruss Dylan 2-O, 3-O C Cabrera CHA 168-P Cabrera Olga Lucía 7-O, 9-O, 10-O Cáceres Abraham G 15-O Cadavid Gloria 73-O Cadena Horacio 26-O, 8-P, 63-P, 159-P Caligiuri Lorena G 3-P Calvopiña Manuel 162-P Calzada Jose E 35-P Camargo-Neves Vera 172-P Cameron Mary 46-P Campino L 33-P, 57-P, 111-P Campos Marliane Batista 112-P Canneva Bruno 122-P, 141-P, 142-P, 150P Caranha Lindemberg 89-P Cardoso A 140-P Cardoso Cristiane de Oliveira 17-P Carneiro Mariângela 135-P Carpenter S 50-O, 143-O Carter V 14-O Carvalho AK 97-P Carvalho Gustavo Mayr de Lima 25-P, 136-P, 139-P Carvalho Silvia María Santos 144-P Casanova Claudio 171-P, 173-P Casaril Aline Etelvina 84-P, 85-P, 88-P, 128-P Casertano Sergio A 58-P, 125-P Castilho Regiane de Cassia Hilário 109-P Castillo G 67-P Cavalcanti Milena de Paiva 32-P Cavia Regino 21-P Çetin Hüseyin 4-O Chacon M 27-O, 127-P, 147-P Chagas Anadeiva Portela 30-O Chalghaf Bilel 32-O Chanampa Mariana M 67-P Charrel RN 39-O, 44-O Chaves Sérgio Augusto de Miranda 18-P, 19-P Chavez Luis F 35-P Checa Rocío 22-O, 17-O Chemkhi Jomaa 49-P, 96-P Chelbi Ifhem 3-O, 44-O, 155-P Cherni Saifedine 3-O, 44-O, 155-P Chicharro Cármen 17-O, 22-O

Boletín de la Sociedad Entomológica Argentina Chiluisa Orlando 162-P Chlebi Ifhem 72-O Chlif Sadok 32-O Chouihi E 132-P Ciufolini MG 68-O Cochero Suljey 46-O, 51-O, 91-P, Coelho Tháis Oliveira 6-P Colla-Jacques Fernanda Elisa 173-P Collins Frank H 62-O Contreras Angélica 159-P Copa Griselda N 67-P, 158-P Corbett CEP 97-P Cordeiro Jose LP 42-P Cordovez Juan Manuel 153-P Corrêa Arlene Gonçalves 71-O Cortés Luis 51-O, 91-P Cosenza M 23-O Costa Carlos Henrique Nery 10-P, 123-P Costa Dorcas L 10-P Costa Filho Pedro Catarino 143-P Costa Pietra Lemos 12-P, 30-P Costa Simone M 18-P Courtenay O 14-O, 170-P Cristóvão JM 33-P, 57-P, 111-P Cruz Israel 17-O, 22-O Cruz Susana 22-O Cumbrera Anayansi Valderrama 65-O Cupollilo Elisa 116-P Cutolo Andre Antonio 104-P D Dachraoui K 44-O, 132-P da Costa Ailton Junior Antunes 74-P, 75-P da Costa Simone Miranda 19-P, 42-P da Fonseca Claudio Ruy V 55-P D'Agostino R 23-O Dagur Pradeep K 56-O Damazo Amilcar Sabino 1-P Dantas-Torres Filipe 12-P, 30-P,31-P, 32-P, 152-P, 12-O Darabi H 40-O Darvishi M 40-O Da Paixão Fabiana Rodrigues 75-P da Silva Ana de Nazaré Martins 76-P da Silva Andrea Martins 69-O da Silva Danyele Franca 134-P da Silva Fábio Márcio Medeiros 54-P, 92-P, 112P da Silva Fernando José 12-P, 30-P, 152-P da Silva Jalthon C 10-P da Silva José Hilário Tavares 146-P, 149-P, 156-P da Silva Junior João Batista 118-P, 119-P, 156-P da Silva Kamila Gaudêncio 31-P, 32-P, 152-P da Silva Luiz Osvaldo Rodrigues 89-P

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Da Silva Maria Elisabel Tavares 77-P da Silva Mariana Dantas 104-P da Silva Shara Regina 74-P da Silva Túllio Romão R 94-P Das Pradeep 6-O Davidson Silas 7-P De Almeida Andréa Sobral 135-P de Almeida Paulo Silva 29-O, 143-P de Almeida Romoaldo Martins 143-P de Almeida Tiago Parreiras 27-P, 28-P de Andrade Andrey José 13-O, 29-O, 6-P, 14-P, 50-P, 52-P, 56-P de Araújo Magnólia Florêncio Fernandes 119-P de Araújo Patrick Thiago Carvalho 76-P de Assis Mauro Diego Gobira Guimarães 94-P de Avelar Daniel Moreira 74-P de Carvalho Bruno M 18-P, 19-P, 40-P de Carvalho Gílcia Aparecida 31-P de Carvalho Maria do Socorro Laurentino 29-O de Carvalho RMJS 140-P de Figueiredo Helen Rezende 88-P, 128-P De Lazzari Elisa 1-O de Medeiros Jansen Fernandes 98-P, 129-P, de Melo Fábio Lopes 12-P Demir Samiye 4-O de Moraes Sinara Cristina 80-P, 81-P de Morais Rayana Carla Silva 32-P de Oliveira Alexandra Gutierrez 84-P, 85-P, 88-P, 101-P, 128-P de Oliveira Everton Falcão 88-P Depaquit Jerome 16-O, 18-O, 20-O, 21-O, 24-O, 64-O de Paula Marcia Bicudo 143-P de Pita Pereira Daniela 19-P Derbali Mohamed 3-O, 44-O, 155-P Derrick Tamsyn 55-O de Santana Antônio Luis Ferreira 19-P de Souza Emerson Marques 27-P, 28-P de Sousa Gilberto César 30-O de Souza Jailson Ferreira 17-P de Souza Marcos Barbosa 110-P de Souza, Nataly A 48-P de Souza Wagner Fernnades 84-P, 85-P Deuster Katrin 12-O Dey Ranadhir 56-O Dias Edelberto Santos 1-P, 74-P, 75-P, 79-P, 93-P, 135-P Dias Quezia Nunes Peres 110-P Dias TA 25-P Diaz-Albiter Hector M 67-O Diaz Mery 86-P, 87-P Di-Blasi Tatiana 54-O, 59-O Dilger E 14-O, 170-P Dillon Roderick J 55-O, 67-O

N° 26 (1) 2015 Di Muccio T 45-O Dinesh Diwakar Singh 6-O Direni-Mancini JM 65-P, 163-P Donato Lucas Edel 16-P do Nascimento Thais Brito 81-P Dorval Maria Elizabeth Moraes Cavalheiros 101-P dos Santos Ana Paula de Azevedo 98-P dos Santos Antonio Pereira 89-P Dos Santos Carlos Fernando Rocha 15-P, 77-P dos Santos Eric Fabricio Marialva 129-P dos Santos Fabio Alexandre Leal 27-P dos Santos Klieton Maciel 101-P dos Santos Lucas Heber Mariano 81-P dos Santos Nathanna Progênio 17-P dos Samtos Paulo Raimundo Barbosa 144P dos Santos Thiago Vasconcelos 48-P, 54-P, 76-P, 92-P, 112-P dos Santos Yago Costa Vasconcelos 76-P Driss Mahdi 96-P Duarte Didier Tirado 159-P Duffy Darragh 57-O Duncan Robert 56-O D'Urso V 45-O Dvořák Vít 19-O, 20-O, 21-O, 39-O E Echchakery Mohamed 106-P, 164-P, Echeverría Jorge Espinoza 86-P, 87-P Eguchi Gabriel Utida 84-P, 85-P, 88-P Elnaiem Dia-Eldin A 31-O, 66-O Emrich Scott J 62-O Enriquez Sandra 162-P Ergunay K 39-O Ermis V Ozge 37-P Escobedo-Ortegón Javier 105-P Espada Liz 64-P Estrada Luis G 26-O, 8-P, 63-P F Fadul Mônica 76-P Faiman Roy 39-P Fares W 44-O Faria Maiara d 67-O Farias LHS108-P Farias Teresinha C 10-P Faustino Maria Aparecida da Gloria 31-P Fernándes Carlos Eurico 84-P, 85-P Fernandes Magda Freitas 101-P Fernandes Wedson Desidério 101-P, 124-P Fernández María Soledad 21-P, 43-P, 160-P Fernández Roberto 64-P Fernández Wagner de Souza 88-P Ferrari Júnior Antonio Carlos 101-P Ferreira Fernando 70-P, 71-P

Boletín de la Sociedad Entomológica Argentina Ferreira Gabriel Eduardo Melim 116-P Ferreira Jônatas Barbosa Cavalcante 50-P Ferreira Maria 133-P Ferro Cristina 153-P, 161-P Ferrolho J 111-P Figueiredo Juliana Kindler 109-P Fiuza Vanessa de Oliveira Pires 74-P Fortes-Dias Consuelo Latorre 74-P, 79-P Fortier Daniel Costa 123-P Fortuna C 68-O França-Silva João Carlos 75-P Francesquini FC 97-P Franco Luciano 133-P Freire Maíra Posteraro 20-P, 94-P Freire Renato Cesar Melo 149-P Freitas FB 33-P Freitas RA 151-P Freitas Vanesa C 48-O Fuenzalida A Denise 65-P, 125-P, 163-P Fuller Douglas O 155-P Fux Blima 13-P Fuya Patricia 153P, 161-P G Galati Eunice Aparecida Bianchi 13-O, 17P, 48-P, 51-P, 52-P, 56-P, 70-P, 71-P, 88-P, 101-P, 104-P, 143-P, 171-P Galindo B 147-P Gállego M 145-P, 154-P Gálvez Rosa 17-O, 22-O Galucci Estela 70-P, 71-P Gama Renata Antonaci 149-P Gamarra Roberto M 124-P Garcez Lourdes María 30-O Garciá Ana Lineth 86P, 87-P Garlapati Rajesh B 2-O Garrido Renata 18-P Gaudêncio Kamila 30-P Gebre-Michael Teshome 33-O, 34-O, 35O, 36-O, 37-O, 38-O, 29-P, 34-P, 39-P, 41P, 90-P Gebresellassie Araya 33-O, 34-O, 36-O, 39-P, 41-P, 90-P Gendernalik Alex 9-P Genta Fernando A 67-O Gharbi M 132-P Gil José 67-P, 158-P Giordano Cristina 19-P Giraldo-Calderón Gloria I 62-O, 63-O Giraud Emile 55-O Godoy Rodrigo Espíndola 48-P, 54-P Gomes J. ID 111-P Gomes Suellem Petilim 88-P Gómez Bravo Andrea 44-P Gómez-Bustamante Marinely 86-P, 87-P Gómez Hernando 8-P Gonçalves Raquel 30-O

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Gonçalves Rodrigo Gurgel 6-P Gontijo Célia Maria Ferreira 25-P, 66-P, 95P, 134-P, 139-P, 174-P González-Britez Nilsa 11-P, 133-P González Camila 153-P, 161-P González E 145-P González Estela 99-P, 130-P, 131-P Goulart Thais Marchi 71-O, 113-P, 114-P Gould Ignacio Tomás 5-P, 43-P, 44-P, 125-P Gradoni Luigi 73-P, 45-O Gramiccia Marina 73-P, 45-O, 68-O Grant William E 5-O Graser Carina 18-P Guerbouj Souheila 49-P Guimarães Ricardo 30-O Guimarães Vanessa Cristina Fitipaldi Veloso 12-P, 30-P, 144-P Guizani Ikram 49-P Gunay 39-O Gurgel-Gonçalves Rodrigo 29-O, 14-P, 50P, H Habboul Z 132-P Hadhri MH 132-P Hailu Asrat 19-O, 33-O, 34-O, 29-P, 34-P, 35-O, 36-O, 37-O, 38-O, 39-P, 41-P, 73-P, 90-P Halada Petr 20-O Hamida Nabil Belhadj 58-O Hamilton J Gordon C 14-O, 72-O Harrabi Mariem 32-O Hasan Milena 57-O Hashiguchi Yoshihisa 15-O Hassan Amir 112-O, 176-P Hassan Faizan 6-O Hawksworth Mallory 63-O Hermes Sandra Cristina Negreli Moreira 29-O Hernández Sonia 130-P, 131-P Hiramoto Roberto Mitsuyoshi 109-P Hlavacova J 60-O Hlavačková Kristýna 19-O, 20-O I Ilango Kandan 47-P Infran Jucelei de Oliveira Moura 84-P, 85P, 88-P, 128-P Intile S 82-P, 23-O Ipe Ipe M 43-O Ishikawa Edna Aoba Yassui 76-P J Jafari R 40-O Jiménez Maribel 4-P, 99-P, 130-P, 131-P, 145-P Joshi Amritanshu 56-O Juan Laura W 5-P

N° 26 (1) 2015 K Kahime Kholoud 106-P, 164-P, 165-P, 167-P Kamhawi Shaden 53-O, 56-O, 115-P Karakus Mehmet 4-O, 36-P Kasap Ozge Erisoz 21-O, 39-O, 157-P Kassahun Aysheshm 19-O Katakura Ken 15-O Kato Hirotomo 15-O Kelly-Hope Louise 8-O, 28-O, 176-P Khajeian M. 40-O Khalid Noteila M 66-O Khoury C 45-O, 68-O Kindlová Michaela 73-P Kirstein Oscar 33-O, 34-O, 36-O, 39-P, 41P, 90-P, 162-P Kobylinski Kevin 7-P Košťálová Tatiana 73-P Kowalewski Martín 148-P Kozhahmetova Madina 9-P Kratochvílová Tereza 73-P Kumar Vijay 6-O Kwakye-Nuako G 100-P L Lacerda Helio Márcio Nunes 78-P Lagarmilla Patricia 122-P, 141-P, 142-P, 150-P Lal Das Murari 1-O Lal Dinesh 43-O Lamballerie X de 44-O Lana Rosana Silva 79-P Lara-Silva Fabiana de Oliveira 74-P, 75-P, 79-P La Russa F 82-P Lastre Natalia 59-P Laurenti MD 97-P Lavitschka Cecilia Oliveira 104-P Lawson Daniel 62-O Lazari Thaís Fernanda 171-P Leão Edna De Freitas 112-P Léger Nicole 16-O, 24-O Lehrter Veronique 20-O Leite Keily Cristiny Azevedo 78-P Lelli R 82-P Lemma Wossenseged 33-O, 35-O, 29-P, 34-P Levay Ana Paula Silva 101-P Libri Valentina 57-O Lima Ana Cristina Vianna Mariano da Rocha 74-P, 75-P, 174-P Lima Fernanda Pinheiro 13-P Lima Jan108-P Lima José Aprígio Nunes 112-P Lima Magliones Carneiro 15-P, 77-P Limam S 132-P Lisi O 45-O

Boletín de la Sociedad Entomológica Argentina Lizarralde de Grosso MS 163-P Lopes Josiane Valadão 75-P Lopes Tamy Elicia da Silva 118-P, 119-P, 146-P, 149-P, 156-P Lopez Marla 153-P Lorini Maria Lucia 40-P Louzir Hechmi 57-O, 58-O Lozano Alejandra 122-P, 141-P, 142-P, 150-P Lubbad MY 117-P Lugo Ligia 153-P, 161-P Luna R 127-P LL Llanes-Acevedo Ivonne Pamela 17-O, 22-O Llanos A 27-O M Macena Ana Lúcia Moreira 15-P, 77-P Machado Caroline Amaral 22-P, 116-P Machado Vicente Estevam 71-O, 113-P, 114-P Machado-Coelho George Luiz Lins 74-P Maia C 33-P, 57-P, 111-P Magalhães Mônica de Avelar Figueiredo Mafra 18-P Mandal Goutam 115-P Mandal Srotoswoti 115-P Marceló Catalina 9-O Marchi A 68-O Marchini Fabricio K 59-O, 69-O Marco JD 158-P Margonari Carina 13-P Maroli M 45-O Mas-Coma Santiago 18-O Massoudi Nabil 132-P Mastrángelo AV 11-O Martín-Martín Inés 4-P, 99-P, Martín Ohiane 17-O, 22-O, 55-O Martín-Sánchez J 154-P Martínez Lily Paola 46-O, 51-O, 91-P Martínez Mariela F 148-P Martínez Nidia 133-P Martins Juliana 79-P Mateus Nathália Lopes Fontoura 88-P Mathis Alexander 20-O Matthew Albert 57-O Mazloumi Gavagni A 61-P McCarthy Christina B 3-P McCoy John Philip 56-O McDowell Mary Ann 61-O, 63-O, Meddeb-Garnaoui Amel 57-O, 58-O Medley Graham 170-P Meira Antônio de Medeiros 110-P Melo Alan Lane 80-P Memish Ziad 28-O Mendes Adauto Rodrigues 128-P

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Mendes Ileane Gurgel 119-P Mendonça Ivete L 10-P Mendoza J 127-P Menegatti Jaqueline Aparecida 29-O Meneghello Brígida Helena da Silva 109-P Meneses Claudio 115-P, 70-O, 56-O Menezes Filho Antônio 10-P Menezes Júlia Alves 13-P Menz Ingrid 104-P Mermer Ahmet 37-P Mhadhbi M 132-P Michalsky Érika Monteiro 74-P, 75-P, 79-P, 93P Mierez Mirta Liliana 62-P Miguelañez Silvia 17-O, 22-O Miranda Débora Elienai de Oliveira 31-P, 32-P, 152-P Miró Guadalupe 17-O, 22-O Miyazaki Rosina Djunko 26-P, 27-P, 28-P, 93-P Molina Juan 11-O, 5-P Molina Ricardo 4-P, 99-P, 130-P, 131-P, 145-P Molla Leticia María 104-P Monaco Neiva Zandonaide 88-P Moncaz Aviad 33-O, 34-O, 36-O, 90-P Mondragon-Shem Karina 28-O, 46-P, 176P Montesino Ana 51-O, 91-P Montoya Ana 17-O, 22-O Moraes Carolina d. 67-O Morais María Helena Franco 135-P Morais Mayron Henrique Gomes 13-P Morales E 70-O Morilla F 154-P Moreno Eduardo Stramandinoli 20-P Moreno H Claudia Ximena 73-O Mosqueda Luis Armando 62-P Moura Abilene Cristina de Arruda 32-P, 152-P Mukhopadhyay Rita 115-P Műller Ingrid 55-O Murgas Ingrid 65-O N Nakashi Hira L 56-O Naouar Ikbel 57-O, 58-O Nascimento Lucimar 172-P Nascimento Luis Otávio Cordeiro 20-P Nasser Julio R 67-P, 158-P Negrão Fábio Juliano 101-P Neto Grimoaldo Braga Da Rocha 15-P, 77P Nogueira Fabio dos Santos 104-P Novo Shênia Patrícia Corrêa 110-P Nowling RJ 63-O Nzelu Chukwunonso Onyemaechi 15-O

N° 26 (1) 2015 O Obara Marcos Takashi 6-P Oliveira AFJ 151-P Oliveira Alessandra G 124-P Oliveira Ana Flávia de Morais 78-P Oliveira DMS 108-P OliveiraEverton Falcão 128-P Oliveira Fabiano 53-O, 70-O Ölgen M. Kirami 4-O Oreste F 67-P Orta Jose 115-P Ortega Edgar 26-O, 8-P, 63-P Ortiz Washington Benitez 162-P Oscherov EB 38-P Oshiro Elisa Teruya 84-P, 85-P, 88-P, 101-P, 128-P Osuguku E 27-O Otranto Domenico 12-O, 152-P Ouanaimi Fouad 106-P, 164-P, 165-P, 166-P, 167-P Ovallos Fredy Galvis 70-P, 71-P, 104-P, 173P Özbel Y 4-O, 39-O, 36-P, 37-P Özkul A 39-O P Pacheco R 27-O, 102-P, 103-P, 126-P, 127P, 147-P Pandey Shubham 43-O Paranhos Filho Antonio C 124-P Pardo Raúl H I 7-O, 9-O, 10-O Parente Daniela M 10-P Parrado Rudy 86-P, 87-P Parras Matías Ariel 23-P, 24-P, 68-P, 69-P, 125-P ParreiraR. 57-P Passero LF 97-P Paternina Luis 46-O Paternina Margaret 46-O Paz Gustavo Fontes 139-P Pech-May Angélica 105-P Pereira AAS 174-P Pereira Araujo T 102-P Pereira-Chioccola 104-P Pereira Júnior Antonio Marques 98-P, 129-P Pérez Adriana Alicia 11-O, 43-P Pérez-Doria Alveiro 46-O, 51-O, 59-P, 91-P Perez JE 27-O, 103-P, 126-P, 127-P PessanhaJosé Eduardo Marques 74-P Pessoa Felipe Arley Costa 98-P, 129-P, 151P Pessoa Grasielle CD 16-P Pesson B 154-P Pflüger Valentn 20-O Philips-Singh Doris 43-O Piazza M 23-O Picado Alberto 1-O

Boletín de la Sociedad Entomológica Argentina Pinheiro Marco Paulo Gomes 118-P, 119-P, 120-P, 146-P, 149-P, 156-P Pinheiro Maria Paula de Melo Pereira 120-P Pinheiro Maria Sueli Barros 92-P, 112-P Pinto Israel de Souza 116-P Pinto Mara Cristina 71-O, 113-P, 114-P Pimenta Paulo PFP 48-O, 52-O Pitaluga Andrè N 54-O, 69-O Pita-Pereira D 102-P Pires Ana Clara Araújo Machado 52-O Pitti R 23-O Poché David M 5-O, 9-P Poche Richard 2-O, 3-O, 9-P Polyakova L 3-O, 9-P Ponte Cesar do Santo 110-P Portillo Nilda 133-P Portús M 145-P, 154-P Posada Laura 46-P Posada-López Laura 73-O, 63-P, 159-P Pozo Catherine 162-P Prudhomme Jorian 20-O Pruzinova K 60-O Q Quaresma Patrícia Flávia 25-P, 60-P, 72-P, 95-P Qualls Whitnay A 155-P Quintana María Gabriela 11-P, 65-P, 163-P, 168-P Quispe Anita 27-O, 103-P, 126-P, 147-P Quispe C 147-P Quispe D 27-O, 126-P Quispe-Florez MM 102-P, 103-P Quispe M 27-O Quispe-Ricalde MA 103-P, 147-P R Rado D 27-O, 102-P, 103-P, 126-P, 127-P, 147-P Rahola Nil 53-P Ramalho Miriele 152-P Ramalho-Ortigao José Marcelo 59-O, 115P Randrianambinintsoa Fano José 16-O Rangel Elizabeth F 16-P, 18-P, 19-P, 40-P, 42-P, 48-P, 80-P, 89-P, 102-P, 126-P Raizer Josué 29-O Ravel C 145-P Rea María Josefa Felisa 62-P, 121-P Ready PD 47-O Reale S 23-O, 82-P Rebai-Kammoun Wafa 57-O, 58-O Rebolar-Téllez Eduardo A 105-P Remondegui CV 168-P Rêgo Felipe Dutra , 25-P, 94-P, 95-P, 116-P, 134-P, 138-P, 139-P Rego Taiana Amâncio da Costa 19-P

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Reis AS 66-P, 174-P Reithinger Richard 86-P Remoli ME 68-O Rhodes C 70-O Ribeiro Ana Lucia Maria 1-P, 26-P, 27-P, 28-P, 93-P Ribeiro Carlos CB 10-P Ribeiro Leticia Moraes 128-P Rigg Chystrie 35-P Ríos Cristina 122-P, 141-P, 142-P, 150-P Rocha Anárya Teresa de Freitas 123-P Rodarte Cláudia Cavalcante De Matos 77P Rodrigues Eduardo Henrique Gomes 12-P Rodrigues Jorge Senatore Vargas 1-P, 26-P, 27-P, 28-P Rodrigues Moreno de Souza 129-P Rodríguez Jorge Luis 51-O Rodríguez-Jiménez Jorge 91-P Rocha Douglas de Almeida 6-P, 50-P Rogers Matthew E 55-O Rohoušová Iva 73-P Rojas Agustina 25-O Rojas de Arias Antonieta 11-P Romero Luis Roberto 51-O Romero Selva 122-P, 141-P, 142-P, 150-P Romero Vanesa 162-P Romero-Ricardo Luis 59-P, 91-P Rosa Juan Ramón 23-P, 24-P, 38-P, 68-P, 69-P Routray Parimita 46-P Rowland Mark 1-O Rowland Tobin 7-P Rowton Edgar 7-P Ruiz-Lopez Freddy 159-P Rugani Jerônimo Marteleto Nunes 25-P, 95P, 174-P S Sábio Priscila Bassan 51-P, 52-P, 56-P Sabroza Paulo Chagastelles 135-P Sadlova Jovana 49-O, 50-O Saeidi Z 61-P Salah Afif B. 32-O, 57-O, 58-O Saldaña Azael 35-P Salomón Oscar Daniel 11-O, 3-P, 5-P, 11-P, 21-P, 23-P, 24-P, 38-P, 43-P, 44-P, 45-P, 58P, 65-P, 68-P, 69-P, 83-P, 125-P, 148-P, 160-P, 163-P, 175-P Samantaray Sachidananda 2-O Sánchez-García Laura 105-P Sandoval Adolfo Enrique 45-P, 225-P Sanguinette Cristiani de Castilho 134-P, 136-P Santamaría Erika 7-O, 9-O, 10-O Sant'Anna Mauricio R 67-O Santini María Soledad 11-O, 3-P, 11-P, 21-

N° 26 (1) 2015 P, 45-P, 148-P, 160-P, 175-P Santos Fabricio K. M. 16-P, 89-P Santos Luciene Aranha da Silva 112-P Santos Mirella Ferreira da Cunha 124-P, 128-P Santos Nathália Queiroz 76-P Santos TV 108-P Santos Walter Souza 30-O Saracho-Bottero MN 163-P Saraiva Lara 66-P, 136-P, 174-P Satragno Dinora 122-P, 141-P, 142-P, 150-P Scholte Ronaldo Guilherme Carvalho 95-P Sciamarelli Alan 29-O Sebastien Boyer 16-O Seblova-Hrobarikova Veronika 49-O, 50-O Secundino Nágila FC 48-O, 52-O Sequeira Cirino 141-P, 142-P, 150-P Ser Önder 4-O Serra e Meira Paula Cavalcante Lamy 136-P, 137-P Sevá Anaiá da Paixão 70-P, 71-P Severini F 45-O Shahbazi F 40-O Shaw Jeffrey 41-O Shenker Moshe 33-O Shimabukuro Paloma Helena Fernandes 13-O, 20-P, 22-P, 55-P, 94-P, 95-P, 116-P, 171-P Silva AM 54-O Silva BJM 108-P Silva EO 108-P Silva João Carlos França 79-P Silva Júlia dos Santos 19-P, 89-P Silva Maiara Alves 74-P, 75-P Silva Marcel Miranda de Medeiros 146-P, 149-P, 156-P Silva Rafaella A 16-P Silva Simone Melo 123-P Silveira Fernando Tobias 92-P, 112-P Silveira FT 97-P, 108-P Šíma Michal 73-P Singh Mutum Ingobi 2-O Singh NS ID 43-O Soares Daniela Cristina 30-O Soares Márcia Maria Costa Nunes 109-P Soares Maria Regiane Araújo 123-P Soares Rodrigo Martins 70-P, 71-P Soleimani H 40-O Soto M 103-P, 127-P Sousa Alessandra Mara 13-P Sousa Lindemberg Caranha 16-P, 48-P Souza Daiana Alovisi 84-P, 85-P, 88-P Souza Cristian Ferreira 60-P, 72-P Souza GD 140-P Souza Mielle Barbosa 60-P Stanneck Dorothee 12-O Steinhorst Iris Ingrid 45-P

Boletín de la Sociedad Entomológica Argentina Stell Frederick 64-P Sttump Rodolfo German Antonelli Vidal 134-P Suar Mrutyunjay 46-P Suau Hamide Aslan 55-O, 115-P Sumová Petra 73-P Supparo Eduardo 141-P Szelag Enrique Alejandro 23-P, 24-P, 68-P, 69-P T Tadros Samuel 63-O Tanure Aline 134-P, 139-P, 139-P Tartaglino Lilian 3-P Tavares Mara Garcia 65-O Tekie Habte 35-O, 36-O, 37-O, 29-P, 34-P, 90-P Teles Carolina Bioni Garcia 98-P Telleria Erich Loza 59-O Tempone Antonio Jorge 54-O, 59-O, 69-O Thies Sirlei Franck 1-P, 2-P, 27-P, 93-P Thomaz-Soccol Vanete 11-P Tinoco B 54-O Toccas F 27-O, 127-P, 147-P Tolezano José Eduardo 109-P Tomokane TV 97-P Tonelli Gabriel Barbosa 95-P, 134-P, 139-P Torales Martha 133-P Torina A 82-P Torina S 23-O Tort Cecilia 122-P, 141-P, 142-P, 150-P Toumi Amine 32-O, 57-O, 58-O Töz Seray 4-O Traub-Csekö Yara María 54-O, 59-O, 69-O Tripet Frederic 66-O Trueba Gabriel 64-O U Uribe Sandra I 8P, 26-O, 63-P, 73-O Utgés María Eugenia 11-O, 83-P, 125-P, 148-P, 175-P V Vaca Franklin 162-P Vaccalluzzo V 45-O Valderrama Anayansi 35-P Vale Mariana Moncassim 40-P Valencia B 27-O Valenzuela Jesus 8-O, 28-O, 53-O, 56-O, 70-O, 112-O, 115-P Valladares B 102-P, 103-P, 126-P, 127-P, 147-P, 176-P Vargas Jorge Senatore Rodrigues 61-P, 93-P Vásquez Gisella 64-P Vega Celeste 11-P Vela Gabriela 64-O Vélez Iván Darío 26-O, 8-P, 63-P, 159-P

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Vélez-Mira Andrès 26-O, 8-P, 63-P, 159-P Verbel-Vergara Daniel 46-O Vergara José G 51-O, 91P Verger Lorenzo 122-P, 141-P, 142-P, 150-P Vianna Elisa Neves 135-P Vidal Joaquim 17-P Viera Ana 122-P, 141-P, 142-P, 150-P Vilela M 126-P Villacrés Ernesto 162-P Villarquide ML 160-P Vincent Robert 16-O Vitale Edgardo 122-P, 141-P, 142-P, 150-P Vitale F 23-O, 82-P Vivero Rafael J 26-O, 73-O, 8-P, 63-P Volf Petr 19-O, 20-O, 21-O, 39-O, 49-O, 50-O, 60-O, 73-P Votýpka Jan 19-O, 21-O, 39-O, 49-O, 60-O W Wadsworth Marinha 63-O Wang Hsiao-Hsuan 5-O Warburg Alon 19-O, 33-O, 34-O, 35-O, 36O, 37-O, 38-O, 42-O, 49-O, 29-P, 34-P, 39P, 41-P, 73-P, 90-P, 162-P Ximenes Maria de Fátima Freire de Melo 118-P, 119-P, 120-P, 146-P, 149-P, 156-P Y Yadon Zaida E 11-P Yaghoobi-Ershadi M. Reza 40-O Yared Solomon 33-O, 38-O Yazidi Rihab 96-P Yépez José Yancarlos 25-O Yilmaz Bahtiyar 4-O Z

Zacarias Danielle A 10-P Zanini Volmir 20-P Zaffora G 23-O Zapata Sonia 20-O, 64-O Zenobio Ana Paula Lusardo de Almeida 136-P Zerba Eduardo N 5-P Zhioua Elyes 3-O, 72-O, 44-O, 132-P, 155-P Zoghlami Z 132-P Zorrilla Victor 64-P Zwetsch Adriana 48-P

ABSTRACT BOOK ROUND TABLES & ORAL SESSIONS

ISOPS VIII Puerto Iguazú Argentina

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

ID 1-O Vector Control

ID 2-O Vector Control

EVALUATION OF DIFFERENT NETS AGAINST PHLEBOTOMUS ARGENTIPES, THE VECTOR OF VISCERAL LEISHMANIASIS IN NEPAL

MULTIPLE FIPRONIL DOSES IN DAIRY CATTLE FOR VECTOR CONTROL IN BIHAR, INDIA: RESIDUE STUDY AND EFFICACY DETERMINATION AGAINST THE SAND FLY PHLEBOTOMUS ARGENTIPES

Albert Picado; Murari Lal Das; Mark Rowland; James Austin; Elisa De Lazzari B a rc e l o n a C e n t re f o r I n t e r n a t i o n a l H e a l t h R e s e a rc h ( C R E S I B ) . [email protected]

Keywords: Phlebotomus argentipes, insecticide treated nets, mesh size, cross-over trial

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Rajesh B Garlapati1, Sachidananda Samantaray2, Mutum Ingobi Singh1, Trey Barresi1, Dylan Burruss1 and Richard Poche1 1 Genesis Laboratories, Wellington, Colorado, USA. 2Bihar Veterinary College, Patna, Bihar, India.

[email protected]

Keywords: Fipronil, cow, residue, sand fly In the Indian subcontinent, Leishmania donovani, the parasite causing visceral leishmaniasis (VL) is transmitted by the sand fly vector Phlebotomus argentipes. Long lasting insecticide treated nets (LN) have been postulated as alternative or complement to Indoor Residual Spraying but there are few field studies evaluating the entomological efficacy of different nets against this vector. We conducted two crossover trials in a VL endemic area in Nepal to compare the barrier effect of (1) LN with different mesh sizes (156 holes/inch2 vs 625 holes/inch2) and (2) alpha-cypermethrin treated LN and untreated nets having the same mesh size (156 holes/inch2). Each crossover trial had two arms consisting of a sequence of two different nets for 8 nights. We used 10 cattle sheds per trial as unit of analysis and a cow placed under the net as bait. CDC light traps placed inside the nets were used to evaluate the number of P. argentipes crossing the net barrier. Negative binomial generalized estimating equation (GEE) population-averaged models adjusted by night and sequence were used to estimate the barrier effect of the different nets. The crossover trials conducted in a rural village in Morang district (South-eastern Nepal) demonstrated that reducing the size of the holes in treated nets (625 holes/inch2) increased the barrier effect of LN by 77% (95% confidence interval (CI): 56% - 88%) compared with treated nets with larger holes (156 holes/inch2). Treating nets with alpha-cypermethrin reduced the number of P. argentipes captured inside the nets by 77% (95% CI: 27% - 93%) compared with untreated nets. The effect of finer mesh LN on VL prevention needs to be tested in a randomized controlled trial.

Visceral Leishmaniasis is a protozoan disease prevalent in India. VL is a severe form of Leishmaniasis which is transmitted by sand flies. Phlebotomus argentipes is the only available vector for VL transmission in India. Vector control plays an important role in VL management. Cows are an important blood meal source for P. argentipes. A study was initiated to assess sand fly mortality in response to fipronil oral dosing of dairy cows. Twenty hybrid cows were selected and four different doses (0.25 mg/Kg body weight, 0.5 mg/Kg body weight, 1.0 mg/Kg body weight and 1.5mg/Kg body weight) of fipronil were given at 8 week intervals for six months, totaling three separate applications. Sand fly efficacy and fecundity were evaluated on Day 1, 4, 7, 14, 21, 28, 35, 42 and 49 after treatment. Fipronil residues in milk were assessed for the first 14 days after treatment. Plasma residues were assessed on Day 1, 4, 7, 14, 21 and 28 after treatment during three applications. Milk production, cow health and environmental conditions were monitored during the study. Higher doses of fipronil (1.0 mg/Kg body weight and 1.5 mg/Kg body weight) had high residues in milk and longer sand fly efficacy after treatment. The efficacy of the lowest dose (0.25 mg/kg body weight) was found up to 14 to 21 days after treatment. Fipronil did not have a significant effect on sand fly fecundity. Metabolism of cows played an important role in milk and plasma residues during three applications. The study results suggest that the use of fipronil as a feed through substance can be incorporated into Visceral Leishmaniasis management programs.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

ID 3-O Vector Control

ID 4-O Vector Control

LABORATORY AND FIELD EVALUATION OF RODENT BAIT TREATED WITH FIPRONIL FOR FEED THROUGH AND SYSTEMIC CONTROL OF PHLEBOTOMUS PAPATASI

N AT U R A L I N F E C T I O N A N D I N S E C T I C I D E SUSCEPTIBILITY STATUS OF WILD CAUGHT SAND F L I E S I N R U R A L A R E A S O F A N TA LYA , MEDITERRANEAN REGION OF TURKEY

M Derbali1, L Polyakova2, A Boujaâma1, D Burruss2, S Cherni1, W Barhoumi1, I Chelbi1, R Poch2, E Zhioua1

Mehmet Karakuş1; Samiye Demir2; Hüseyin Çetin3; Suha Kenan Arserim4; Önder Ser5; Seray Töz6; I Cüneyt Balcioğlu7; M Kirami Ölgen8; Bahtiyar Yilmaz2; Yusuf Özbel6

1

Institut Pasteur de Tunis, Laboratory of vector Ecology, 13 Place Pasteur, BP 74, 1002 Tunis, Tunisia. 2Genesis Laboratory Inc., 10122 NE Frontage Road, Wellington, Co 80549, USA. [email protected]

Keywords: Phlebotomus papatasi, Meriones shawi, zoonotic cutaneous leishmaniasis, systemic and feed through insecticide The sand fly Phlebotomus papatasi is the main vector of Leishmania major, etiologic agent of zoonotic cutaneous leishmaniasis (ZCL), which is endemic in North Africa, the Middle East, and Asia. In North Africa, Meriones shawi is one the main reservoir host of L. major. Phlebotomus papatasi populations are maintained in borrowing rodents such as M. shawi. Three fipronil-treated rodent baits were evaluated for systemic and feed through insecticidal activity against P. papatasi feeding on M. shawi. Through blood feeding bioassays, mortality rates of females P. papatasi increased with the concentration of fipronil in the rodent bait varying from 0.001% to 0.005%. In the laboratory, more than 90.0% of P. papatasi were killed within 48 hours after blood feeding on the desert's jirds, Meriones shawi, treated up to 29 days prior with a single application of fipronil at a concentration of 0.001%, 0.0025% and 0.005%. Through larval bioassays, mortality rates of larvae that have fed on faeces of treated bait for M. shawi increase with the concentrations of fipronil. Faeces of orally-treated Meriones were significantly toxic to larvae for 5 weeks with a concentration of 0.005%. In the field, application of treated bait resulted in 80.0% reduction in the populations of P. papatasi up to 6 weeks after a single application of fipronil at a concentration of 0.005%. This is the first study to demonstrate field efficacy of fipronil-treated rodent baits for P. papatasi control and the first study to evaluate this approach in M. shawi, a principal ZCL reservoir host. These results suggest that fipronil-treated rodent baits can be used to effectively reduce the populations of P. papatasi associated with M. shawi in ZCL endemic areas.

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1 Ege University Institute of Health Sciences, Bornova, İzmir, Turkey; 2Ege University Faculty of Science, Department of Zoology, Bornova, İzmir, Turkey; 3Akdeniz University Faculty of Science, Department of Biology, Antalya, Turkey; 4Celal Bayar University Vocational School of Health Sciences, Manisa, Turkey; 5Ministry of Health Public Health Agency, Antalya, Turkey; 6Ege University Faculty of Medicine, Department of Parasitology, Bornova, İzmir, Turkey; 7Celal Bayar University Faculty of Medicine, Department of Parasitology, Manisa, Turkey; 8Ege University Faculty of L i t e r a t u re , D e p a r t m e n t o f G e o g r a p h y, B o r n o v a , İ z m i r, Tu r k e y. [email protected]

Keywords: Insecticide, Bednet, Antalya, Turkey Sand fly-borne diseases like visceral, cutaneous leishmaniasis and phlebovirus infections have been seen endemically in Turkey. The application of insecticides is mainly targeting mosquitoes/flies control. However, no study was performed about the susceptibility of sand flies against pyrethroids in Turkey. This study was carried out in Antalya province located in The Mediterranean Region of Turkey and we aimed to determine (1) the sand fly fauna, (2) the susceptibility of wild caught sand flies, mainly collected from rural areas, against commonly used pyretroids and (3) natural infection of Leishmania. CDC's bottle bioassay has applied for pyrethroid susceptibility by using different dosages. The tests were repeated 5 times with 100 specimens per bottle. In addition, WHO's Cone test were performed using two different bed nets (Olyset Plus® with PBO and Permanet®) to understand their efficacy. Following the standards of WHO testing procedures, all specimens were dissected, mounted and identified using Mediterranean keys. After dissection, the rest of the body of the specimens was pooled according to the locations and species and totally 50 pools were generated. DNA extractions were made using commercial kit and previously described conventional PCR of ITS-1 region was performed. A total of 10 Phlebotomus species were found in the province with the abundance of P. neglectus (38.82%), P. alexandri (21.66%) and P. tobbi (20.44%). Totally three different active ingredients were tested and knock down times were calculated using Probit Analysis. Knock down times were noted with 10min intervals, KdT50/KdT100 were noted for determining the effective dosages. For the lowest dosage of insecticides, KdT100 value were noted as 36 minutes for permethrin (0.05%), 60 minutes for cypermethrin (0.05%) and 42 minutes for deltamethrin (0.05%). By the end of 24-hour period, no sand fly was alive in bottle assay while 46% and 0% of sand flies were alive in cone test-Permanet® and cone test- Olyset Plus®. Four pools containing Phlebotomus neglectus and one pool Phlebotomus tobbi were found to be positive for Leishmania spp. In conclusion, no resistance was determined in bottle assay but upcoming resistance with prolonging death time and knock down times were noted. Cone test results have showed that Olyset Plus® has more knockdown effect than Permanet®. Mediterranean Region is one of the important tourism and agriculture centers in Turkey. Related to this the insecticide usage.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

ID 5-O Vector Control

ID 6-O Vector Control

SAND FLY CONTROL IN BIHAR, INDIA: MODELING ALTERNATIVE CONTROL STRATEGIES USING SYSTEMIC INSECTICIDES ORALLY ADMINISTERED TO LIVESTOCK

DEVELOPMENT OF RESISTANCE AGAINST DDT TO PHLEBOTOMUS ARGENTIPES (DIPTERA: PSYCHODIDAE) AND SEARCH FOR ALTERNATIVE INSECTICIDE

David M Poché, William E Grant, Hsiao-Hsuan Wang

Diwakar Singh Dinesh, Faizan Hassan, Vijay Kumar and Pradeep Das

Texas A&M University, Genesis Laboratories, Inc.

Rajendra Memorial Research Institute of Medical Sciences (Indian Council of Medical Research), Agamkuan, Patna-800007, India.

[email protected]

25

[email protected]

Keywords: Bihar, fipronil, Phlebotomus argentipes, simulation model. Approximately 60 percent of visceral leishmaniasis (VL) cases occur in Northern India and bordering regions of Nepal and Bangladesh, where the disease is spread by the sand fly species Phlebotomus argentipes. Bihar is the most poverty-stricken state in India and the majority of VL cases reported in the country occur in this region. DNA extraction suggests that cattle, domestic buffalo, goats, and humans serve as the primary hosts for P. argentipes in villages in Bihar. Recent research in Bihar has indicated that the systemic insecticide fipronil, orally administered to cattle, can kill 100% of adult P. argentipes feeding on insecticide-treated cattle and also kill 100% of larvae feeding on feces from insecticide-treated cattle. In addition, fipronil is a lipophilic compound and has the unique ability to be efficacious for several weeks. These results have suggested that fipronil can serve as a means for controlling sand fly populations and potentially reduce cases of VL in the region. To evaluate the potential efficacy of large scale fipronil treatment, a quantitative model was developed, representing the various sand fly life stages (egg, larva, pupa, and adult) in a typical rural village in Bihar. Seasonal variations in temperature, precipitation and relative humidity were incorporated into the model to better represent P. argentipes seasonality in Bihar within the system of interest. A variety of control strategies were simulated in which we varied the season and frequency of livestock treatment. Simulation results suggest that the potential to reduce the sand fly population in Bihar will depend on coordination of the timing of treatments with particular phases of the P. argentipes life cycle as they relate to sand fly seasonality in Bihar. The simulations also suggest that control strategies should be administered over a multiyear period to achieve preferred results.

Keywords: Sand fly DDT resistant Phlebotomus argentipes (Diptera: Psychodidae) Annandale and Brunetti, the vector of Visceral Leishmaniasis in Bihar, India is developing resistance against the insecticide of choice, i.e. DDT. The measurement of tolerance/resistance was based on tube-bioassay test with 4% DDT impregnated paper to the laboratory bred newly emerged unfed and blood fed along with wild caught fed female P. argentipes. Variable results were found showing 40-60% resistant population in all tested P. argentipes. This was verified and compared with changes in Glutathion S- transferase (GST) enzyme (major detoxifying enzyme for xenobiotics) at protein level. The diagnostic band of 42 kD was found highly expressed among the resistant group by the 12% SDS- PAGE in the lab colonized and wild caught P. argentipes that was also confirmed as GST through Western Blot. Other enzymes like Acetylcholinesterase, Esterase and Mono oxygenase enzymes are under process of evaluation. Further, alternative insecticide like Deltamethrin was tried and found very effective in killing P. argentipes in tube bioassay test conducted with 0.05% WHO impregnated paper corresponding with the result of indoor residual spray 20mg/m2. This study will lead in finding actual number of resistant population along with the use of an alternative effective insecticide to control transmission of the disease.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

ID 7-O Vector Control

ID 8-O Vector Control

T O X I C A N D B E H AV I O U R A L E F F E C T S I N EXPERIMENTAL HUTS OF LONG LASTING INSECTICIDE TREATED NETS AGAINST LUTZOMYIA LONGIFLOCOSA IN THE SUB-ANDEAN RURAL ZONE OF COLOMBIA

A CONTROL STRATEGY FOR OLD WORLD CUTANEOUS LEISHMANIASIS OUTBREAKS

Erika Santamaría, Olga Lucía Cabrera, Raul H Pardo Grupo de Entomología, Instituto Nacional de Salud, Bogotá. Grupo de Entomología y Enfermedades Transmitidas por Vectores, Universidad de La Salle, Bogotá. [email protected]

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Waleed Al Salem1,2*, Louise Kelly-Hope1,3, Maha Abdeladhim4, Salah Balgonaeem2, Mohammed Al Zahrani2, Jesús Valenzuela4, Álvaro Acosta-Serrano1,5 1

Parasitology Department, LSTM, England, 2Saudi Ministry of Health, Riyadh, Saudi Arabia, 3Neglected Tropical Disease Centre, LSTM, England, 4Vector Molecular Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Maryland, USA, 5Vector Biology Department, LSTM, England. [email protected]

Keywords: Lutzomyia longiflocosa, long lasting insecticide treated nets, efficacy, experimental hut

Keywords: Old world cutaneous leishmaniasis, Saudi Arabia, Al Ahsa

The aim of the study was to evaluate the toxic and behavioral effects of long-lasting insecticide-treated nets (LLNs) against Lutzomyia longiflocosa in the sub-Andean rural zone of Colombia in the first experimental hut designed for sand flies. Three bednets were tested: Permanet 2.0 (a.i.: deltamethrin, 55 mg/m2), bednet treated with Icon Maxx (a.i.: lambda-cyhalothrin, 60 mg/m2), and control (untreated bednet). Several holes were made on the bednet to simulate the conditions of a torn net. In each hut, two volunteers were located inside the bednet. The study design was a 3 x 3 multiple Latin square. The recorded variables were: number of sand flies inside the hut and within the exit traps, human landing rate, and mortality. The detected effects of the LLNs on L. longiflocosa were: 1) Inhibition from entering the net: the percentages of females found inside the nets treated with Icon Maxx (13.9%) and Permanet (2.0, 44.8%) were significantly lower compared to the control (77.1%). The Icon Maxx showed the greatest difference in percentage of females inside the net compared with the control, 62.3% (59.0 - 69.4), twice the difference in percentage of females found inside the Permanet, 32.3% (27.7- 36.9). 2) Landing inhibition: The mean human landing rate was 10 times [4.9 females/night/2 persons (f/n/2 p)] and three times (15.1 f/n/2 p) lower in the Icon Maxx and Permanet (2.0) respectively, compared with the control, 49.5 f/n/2 p. The protective effect of the Icon Maxx (90.1%) was higher compared to the Permanet (69.5%. 3) Repellency: the mean exit rate of L. longiflocosa was significantly higher in the Icon Maxx (14.1 f/n) compared to the control (5.1 f/n). Furthermore, the percentage of resting females on the walls of the hut with the LLNs (using as denominator the total number of females outside the net) was significantly lower with the Icon Maxx (23.3%) and Permanet (30.5%) compared to the control (72.3%. 4). Mortality: Mortality at 0 h post-test with the Icon Maxx was almost twice (52.5%) that recorded with the Permanet (28.6%). Nevertheless, mortality at 24 h was similar for both LLNs (78.8% and 82.6%) respectively, without significant differences. The two LLNs showed strong effects on L. longiflocosa behavior and mortality. However, the Icon Maxx seems better because of its quicker lethal effect, higher protective effect, inhibition from entering the net and repellency.

Old World cutaneous leishmaniasis (CL) is one of the most prevalent vector-borne diseases in the East Mediterranean Region, including the Kingdom of Saudi Arabia (KSA). A combination of factors is responsible for the sustained spread of CL throughout this region, including lack of health education, socio-economic factors, civil unrest, and human migration. Furthermore, uncontrolled urbanization and lack of governmental sector integration (i.e. Ministries of Health and Agriculture, and Municipalities) impede the establishment of a coordinated disease control strategy. Our case study focused on a new construction site in a remote area of Al Ahsa governorate (Eastern KSA), which had not been previously assessed for CL by the local leishmaniasis control team. This region is known for being exclusively endemic for zoonotic CL (1, 2). Prior to the start of the leishmaniasis transmission season (~April 2012), 150 (nonlocal) construction laborers, with no previous history of CL, arrived at the construction site. In January 2013, ~60% of the construction laborers were reported to have acquired CL, and further PCR-RFLP analysis confirmed they were infected with Leishmania major (2). Furthermore, the same cohort of patients presented higher serum levels of anti-SP32 antibodies (a known Phlebotomus papatasi saliva marker (3)) compared to infected local residents living in neighboring CL-controlled areas. Over the course of 2013 (March-November), an integrated disease control strategy, conducted by several governmental sectors, which consisted of rodent (mechanical) and vector control, was applied in and around the construction site. Mechanical control involved the removal of Hammada elegans (rodent food plant) and destruction of rodent burrows within a radius of 500 m around the construction area. In addition, vector control was employed by spraying deltamethrin into rodent burrows and wall cracks. Since the implementation of this integrated control strategy, no CL cases have been reported at the construction area up until April 2014.This case study suggests that (i) implementation of an integrated vector and rodent control program is sufficient to overcome outbreaks of zoonotic Old World CL and, (ii) assessing potential disease transmission areas prior to the establishment of new development, is essential to prevent CL outbreaks.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

27

ID 9-O Vector Control

ID 10-O Vector Control

LONG LASTING INSECTICIDE TREATED NETS DECREASE SANDFLY ABUNDANCE AND BLOOD FEEDING SUCCESS INDOORS IN THE SUB-ANDEAN REGION OF COLOMBIA: RESULTS OF A HAMLET VECTOR CONTROL TRIAL AFTER ONE YEAR FOLLOW UP

FIRST EXPERIMENTAL HUT TO STUDY THE EFFECT OF INSECTICIDE CONTROL MEASURES AGAINST SANDFLIES (DIPTERA: PSYCHODIDAE) Olga Lucía Cabrera, Erika Santamaría, Raúl H Pardo Instituto Nacional de Salud

Raúl H Pardo, Olga Cabrera, Catalina Marceló, Erika Santamaría

[email protected]

Universidad de La Salle. [email protected]

Keywords: experimental hut, sandflies, control

Keywords: control, Lutzomyia longiflocosa, bednets

Experimental huts have been used to evaluate the effects of insecticide control measures against mosquitoes indoors mainly for malaria vectors. Such huts have not been used for sand flies. The aim of this study was to build an experimental hut in which to evaluate insecticide control measures against sand flies. The study was carried out in the sub-Andean coffee area of Colombia in an endemic area for cutaneous leishmaniasis (CL) where Lutzomyia longiflocosa is abundant. The project was conducted in three parts: 1) Avenues of entrance and exit. To identify the main avenues of entrance and exit into a rural house, two observational studies on the entering and exiting behavior of sand flies were conducted. The resulting data were used to determine trap placement in the experimental hut. For entrance sites the numbers of sand flies attempting to enter the house through large openings (sticky trap catches) and narrow slits (cage trap catches) were compared; while for exit sites the numbers of blood-fed females previously released within the house, trying to escape through the openings described above were compared. The results from both experiments indicate that L. longiflocosa enter (95% of 313 females) and exit (96% of 177 females) the house mainly by large openings between the ceiling and walls; 2) Trap selection. To select the type of traps to use in the hut, laboratory tests were conducted with Lutzomyia longipalpis in a wind tunnel. Two traps, modified for collecting sand flies were evaluated based on their ability to allow the sand flies to enter and prevent them from exiting the trap. The results show that the horizontal slit trap was the best trap, allowing 84.1% of females to enter and preventing 87.3% of those that entered from escaping; 3) Hut construction. Based on the afore mentioned results, three experimental huts were built in the field. Each hut was a 2.5x2.5x2.3 m room made of bricks with metal roof, ceiling and two large openings in each wall, between the ceiling and the wall, at which to locate the horizontal slit trap system. Special care was taken to ensure to block all other possible escape sites; and 4) Experimental hut test. Hermeticism was evaluated by releasing 300 females inside each hut. After eight hours 70% of the released females were recaptured. Observations of human landing catches of L. longiflocosa inside the experimental huts compared with a control house (hut without traps) showed that L. longiflocosa entered the experimental huts in lower numbers than the control house. By adding baffle traps to the entrances of the experimental huts it was shown that sand flies entered the experimental hut and the control in similar densities.

The aim of the study was to evaluate the entomological effect of long lasting insecticide treated bednet (LLNs) against Lutzomyia longiflocosa, a presumed main vector of cutaneous leishmaniasis in Colombia. The study was carried out in the mountainous coffee area of Huila department. The evaluated variables were: sandfly female abundance, blood feeding rate, feeding success (more than 75% of abdomen filled with blood) and mortality. The study was carried out at the hamlet level using a randomized block design, were 7 triplets of hamlets were formed based on pre-intervention sandfly abundance. Within each triplet three treatments were assigned: (i) Permanet (industrially treated), (ii) Icon Maxx (home treated) and (iii) control (untreated bednet). The study had three phases: 1) baseline study. 21 selected hamlets were visited to collect pre-intervention data, including sandfly abundance. Sandflies were collected by CDC light traps in at least 50% of houses and forest, 2) Intervention. Around 10% of the highest sandflies abundance houses were selected as representative for each hamlet, all beds in each hamlet were covered with a bednet, and 3) pos-intervention. Sandflies abundance was monitored at 1, 6 and 12 month time-points pos-intervention. Preintervention L. longiflocosa indoors mean abundance was similar by treatment. Total abundance pos-intervention decrease significantly from 14.7 f/trap/night in the control to 5.0 f/trap/night for Icon Maxx and 4.3 f/trap/night for Permanet. It means a reduction of around 70% in sandfly abundance in each of the LLNs. Comparison for each of the three month time-points showed the same significant reduction. Percentages of blood feeding success for the pre-intervention were significantly different between treatments, so comparisons preintervention vs. pos-intervention were made for each treatment. Percentage of blood feeding success decreased significantly in the LLNs with a reduction of 41.5% for Icon Maxx and 28.1% for Permanet, while the control had a significant increase of 84%. Sandflies abundance in forest was apparently reduced in the treatment with Permanet but it increased in the treatment with Icon maxx. The two LLNs reduce sandfly abundance indoors and blood feeding success in L. longiflocosa. Icon Maxx could be a useful alternative to become untreated bednets and treated bednets with reduced insecticide power to LLNs. Mass killing effect of LLNs against sandflies could not be discarded.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

ID 11-O Vector Control

ID 12-O Vector Control

EFFICACY OF IMPREGNATED BEDNETS AND SPECIES COMPOSITION IN

PREVENTION OF CANINE LEISHMANIOSIS IN NATURALLY EXPOSED DOGS USING A 10% IMIDACLOPRID/ 4.5% FLUMETHRIN COLLAR (SERESTO®)

EXPERIMENTAL HENHOUSES Manteca Acosta Ma, b; Molina Ja; Utgés M Ea, b; Mastrangelo AVb, c; Pérez AAd; Santini M S.b; Salomón ODa, c REDILA a Instituto Nacional de Medicina Tropical (INMeT); b Centro Nacional de Diagnóstico e Investigación en Endemo-epidemias (CeNDIE); c Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); d Departamento de Ecología, Genética y Evolución. FCEyN (UBA).

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Domenico Otranto1; Filipe Dantas-Torres1,2; Emanuele Brianti3; Fabrizio Solari Basano4; Dorothee Stanneck5; Katrin Deuster5 1 University of Bari, Italy; 2Oswaldo Cruz Foundation, Brazil; 3University of Messina, Italy; 4Acroblu s.r.l., Italy; 5Bayer Animal Health GmbH, Germany.

[email protected]

[email protected]

Keywords: impregnated fabrics, vectorial control, sandfly The use of insecticide-impregnated fabrics as preventive tools has become a relevant topic of research in the epidemiology and control of vector-borne diseases because of their ability to reduce vector-human contact and acceptability by end users at the local level in communitybased vector management programs. In this study we used noncommercial curtains with the intent of finding a participative control strategy where users can make and use the curtains in their own community. The project was developed in a rural area (Misiones, northeastern Argentina) where epidemic outbreaks of cutaneous leishmaniasis (CL) occurred in 2004-2005 vectored by Nyssomyia whitmani. The objective was to evaluate changes in sand fly abundance, particularly of Ny. whitmani, in the presence of 10% permethrine-impregnated mosquito-nets under semi-field conditions. Three experimental henhouses were installed on the border of the forest, each with three chickens. The treatments (impregnated curtain, IC) and controls (non-impregnated curtain, NIC; without curtain, WC) were assigned randomly. To simulate incomplete coverage of human dwellings, curtains were hung so as to cover only two sides of the henhouses. During two consecutive nights, a light trap was set inside each henhouse and in a site without chickens (WCH) from 5 pm to 9 am to measure sand fly abundance.). Trials were repeated monthly from February 2012 to January 2013 except for the period April-June when sand fly populations were extremely low. All sand flies were counted to calculate total abundance and a 10% sample of each combination of month and treatment was used to determine species relative abundance. A total of 38,277 sand flies was collected representing eight species )as follows: Ny. whitmani (89.9%), Migonemyia migonei (5%), Pintomyia pessoai (2.7%), Bruptomyia sp. (1.7%), Lutzomyia longipalpis (0.4%); Evandromyia corteleziisallesi, Psathyromyia shannoni and Lutzomyia monticola (85%). The trap index (average number of sand flies collected/trap/night) per treatment was IC (63.6, SEM 21.5) and NIC (160.2, SEM 62.3), both lower than WC (454.2, SEM 160.1) (F3,14=5.6, p=0.005). We did not find differences between IC and NIC, nor between IC and WCH (59.3, SEM 19.2). These results show significant efficacy both in the use of the impregnated and nonimpregnated curtains as preventive barriers to reduce human-sand fly contact.

Keywords: Prevention, canine leishmaniosis, imidacloprid, flumethrin Field evaluations were conducted to investigate the protective efficacy of a collar containing 10% imidacloprid/ 4.5% flumethrin (Seresto®) for the prevention of Leishmania infantum infection in dogs at different time points (2011-2013). Three animal shelters, two in Sicily and one in Apulia, located in hyper-endemic areas for L. infantum with confirmed presence of competent vectors were selected and 340 dogs testing negative by serology (IFAT) and PCR on skin and bone marrow were enrolled. Dogs were randomly allocated to collar-treatment (165) or untreated control (175) group. The collars were applied at the beginning of the transmission seasons according to label instructions and only replaced if lost or required for body weight changes. Blood, bone marrow and skin samples were collected throughout the study and at the final follow-up. Serological, cytological and molecular tests were performed to detect the presence of L. infantum in the different tissues. In Apulia, dogs were ≤6 months of age at enrolment and of various breeds and had not been exposed to sand flies previously. None of the collar-treated dogs tested positive for L. infantum throughout follow-up, whereas 21 of 58 untreated control dogs tested positive throughout the follow-up with five animals also displaying clinical signs at the final time point. The cumulative incidence density rate (IDR) observed in untreated animals was 46.2%. In Sicily, dogs were 2 months to 8 years old at enrollment and of various breeds. Three of 102 dogs in the collartreatment group and 47 of 117 untreated control dogs tested positive throughout the follow-up. The cumulative IDR observed in untreated animals was 60.7%. The collar (10% imidacloprid/ 4.5% flumethrin) was demonstrated to be safe and highly effective (93.4–100%, based on IDRs) in preventing L. infantum infection throughout the season in heterogeneous dog populations under varying environmental conditions. The regular use of treated collars during the sand fly season thus represents a reliable and sustainable strategy for the control of leishmaniasis in dogs living in or travelling to endemic areas.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

ID 13-O Taxonomy & Phylogeny

ID 14-O Taxonomy & Phylogeny

A NEW PROPOSAL FOR THE TAXONOMIC IDENTIFICATION OF PHLEBOTOMINE SAND FLIES (DIPTERA: PHLEBOTOMINAE) IN BRAZIL

SEX PHEROMONES AS A PRE-MATING SPECIES ISOLATION BARRIER IN BRAZIL

1, 3

2

1

Andrey J de Andrade , Paloma H F Shimabukuro , Eunice A B Galati 1

Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, Av. Dr. Arnaldo, 515, Pinheiros, São Paulo, SP, 01246-904, Brazil, 2Centro de Referência Nacional e Internacional para Flebotomíneos, Centro de Pesquisas René Rachou, Instituto Oswaldo Cruz, Av. Augusto de Lima, 1715, Barro Preto, Belo Horizonte, MG, 30190-002, Brazil, 3Laboratório de Parasitologia Médica e Biologia de Vetores, Área de Patologia, Faculdade de Medicina, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, 70910-900, Distrito Federal, Brazil.

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V Carter1, DP Bray1, G Borges-Alves1, E Dilger2, O Courtenay2, R Brazil3 and JGC Hamilton1 1 Centre for Applied Entomology and Parasitology, School of Life Sciences, Huxley Building, Keele University, Staffordshire, ST5 5BG, United Kingdom; 2School of Life Sciences, University of Warwick, Coventry, CV4 7AL, United Kingdom; 3Fundaçao Oswaldo Cruz, Instituto Oswaldo Cruz, Lab Doenças Parasitarias, Rio de Janeiro, Brazil.

[email protected]

[email protected]

Keywords: pheromone, species complex, reproductive barrier Keywords: identification key, taxonomy, type-species, Brazilian sand fly species Currently, there are 269 valid phlebotomine sand flies species recorded in Brazil, which represents the highest diversity of sand flies when compared to other South American countries. Most species (approximately 110) were described between 1930 and 1950 by Brazilian researchers following different classifications and taxonomic proposals. Systematics of sand flies has been debated during the last decades, new classifications have been proposed, and relationships between these species are being studied based on morphological and molecular characters. Dichotomous identification keys are the first step towards specific identification and for Brazilian sand flies the last identification key was published in 2003. The aim of this study is to present an updated new key to indentify sand fly species recorded in Brazil. To produce the key type-species deposited in nine Brazilian institutions were examined. One hundred and sixty nine species including males and females were found. Observations were made regarding the preservation status of the slides - whether the specimens were still visible and labels were adequately presented; the presence of microscopic slides according to the original description; and specimens numbers and typology. All type-species were photographed to build an image dataset. When types were not found, additional specimens of particular collections were examined. Drawings of the genitalia were made using a camera lucida and covered by ink pen. A list of geographical distribution for Brazilian states, including taxonomic synonyms, and a taxonomic catalog were compiled. The key is divided by gender and the species are grouped by morphological characters rather than by phylogenetic relationships. A total of 251 and 179 dichotomies for 258 males and 235 females, respectively, are presented in the key. Researchers of public institutions and health services will be invited to test the taxonomic key, so it can be validated. An interactive version of this key will be produced using the DELTA (Description Language for Taxonomy) software package. It will include all data compiled by us. The DELTA system allows a quick update as new species will be recorded and described in Brazil. Financial support: FAPESP.

Members of the Lutzomyia longipalpis species complex in Brazil can be distinguished through morphological, physiological and biochemical markers. Determining the relative importance of the different members of the L. longipalpis species complex in transmitting visceral leishmaniasis is important for understanding the epidemiology and control of the disease. It has been proposed that the species complex is under an incipient speciation process, and one factor influencing speciation is mate-finding through male sex pheromones. Male L. longipalpis may be one of four possible sex pheromone chemotypes: (S)-9-methylgermacrene-B (9MGB), 3methyl-alpha-himachalene, cembrene 1 or cembrene 2. Previous studies of crosses between different chemotypes carried out in the laboratory showed differing degrees of reproductive isolation. Thus the sex pheromones of L. longipalpis could affect pre-mating behaviour and prevent random mating and gene flow between individuals. However, field evidence for pheromones acting as premating barriers is lacking. We investigated the cross-attraction of L. longipalpis, from two different pheromone chemotypes in Brazil (9MGB from Araçatuba, Sao Paulo State and cembrene 1 from Marajo, Para State). Using experimental chicken boxes and pheromone lures containing either pheromone or blank controls, CDC light traps were used to capture sand flies attracted to the site. Both male and female sand flies were significantly more attracted to their conspecific pheromone chemotype than CDC traps containing no pheromone. Sand flies were not significantly attracted to sex pheromone of the alternative chemotype. These results support the contention that male sand fly sex pheromones act as a pre-mating isolation barrier in the field, reduce non-productive mating encounters and therefore may be one of the strongest influences on speciation in the L. longipalpis complex.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

ID 15-O Taxonomy & Phylogeny

ID 16-O Taxonomy & Phylogeny

IDENTIFICATION OF PERUVIAN SAND FLY SPECIES (DIPTERA: PSYCHODIDAE) THROUGH DNA BARCODES

ORIGINS AND AFFINITIES OF MALAGASY PHLEBOTOMINE SANDFLIES

1

2,3

Chukwunonso Onyemaechi Nzelu , Abraham G Cáceres , Ken Katakura1, Yoshihisa Hashiguchi4,5,6 and Hirotomo Kato1 1

Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan; 2Departamento Académico de Microbiología Médica, Facultad de Medicina Humana, Universidad Nacional Mayor de San Marcos, Peru; 3Laboratorio de Entomología, Instituto Nacional de Salud, Peru; 4Department of Parasitology, Kochi Medical School, Kochi University, Japan; 5Prometeo, Secretaria Nacional de Educacion Superior, Ciencia, Tecnologia e Innovacion (SENESCYT), Ecuador; 6Centro de Biomedicina, Universidad Central del Ecuador, Ecuador.

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Randrianambinintsoa Fano José1; Léger Nicole2; Boyer Sébastien1; Robert Vincent3; Depaquit. Jérôme2 1 Unité d'Entomologie Médicale, Institut Pasteur de Madagascar, Antananarivo, Madagascar. 2Université de Reims Champagne-Ardenne, ANSES, EA4688-USC « VECPAR », Reims, France. 3MIVEGEC, UMR, Montpellier, France.

[email protected]

Keywords: Phlebotominae, Biogeography, Madagascar, Systematics

[email protected]

Keywords: molecular taxonomy, DNA barcode, sand flies, vectors. Understanding the ecological and medical importance of sand flies relies on accurate identification of species. Currently, the identification of phlebotomine sand flies is based on morphological characteristics. However, this traditional taxonomy is laborious, time consuming and often complicated by cryptic species complexes. Molecular approaches have become increasingly employed and promise to be useful taxonomic markers for rapid and reliable species identification. The use of DNA barcodes has been proposed recently as a tool for identification of the species in different animal taxa. In this study, we explored the efficiency of DNA barcode approach, based on cytochrome c oxidase subunit I (COI) sequences, for species identification of Peruvian sand flies, which included major vector species. A total of 89 sand fly specimens belonging to sixteen morphological species and two genera (Lutzomyia and Warileya) were analyzed. We were able to recover and align the target COI fragment from all sand fly species examined. Neighbor-joining (NJ) analysis of the DNA barcode sequences show that each sand fly species formed barcode clusters with tight cohesion that were clearly distinct from those of allied species and were largely congruent with the conventional taxonomy. Thus, the results validated the usefulness of DNA barcoding systems in species recognition in sand flies from Peru.

Until now, the Phlebotomine sand fly fauna (Diptera, Psychodidae, Phlebotominae) of Madagascar remained largely unexplored and unknown according to the lack of autochthonous transmission of leishmaniasis in this country. To date, three phlebotomine sandflies genera including three endemic subgenera have been recorded from Madagascar Island.: fourteen species are already described. Since 2000, field trap phlebotomine sandflies were studied using both morphology and molecular biology (rDNA and mtDNA).One objective is to understand the origins and the affinities of this fauna group that was never studied due to the lack of precise knowledge on the origins of different lineages of these sandflies. At the light of the results obtained including molecular characterization and phylogenies, the authors discuss the endemism concerning Phlebotomine sandflies from Madagascar, the relationships of Malagasy phlebotomine sand flies with species from Africa, America, Oceania and Asia, and propose an hypotheses explaining the settlement of this island by Phlebotomine sandflies, including an old one related to the isolation of Madagascar from the Gondwana following a generalized track compatible for the Malagasy Phlebotomus and probably the Vattieromyia. The morphological affinities of the later subgenus with two Australian species S. pugifera et S. standfasti or with the American Pressatia is discussed. The individualization of the Trouilletomyia and Grassomyia should be younger and underlined several routes of settlement of Madagascar.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

31

ID 17-O Taxonomy & Phylogeny

ID 18-O Taxonomy & Phylogeny

DNA BARCODING OF MEDITERRANEAN PHLEBOTOMINE SANDFLIES BASED ON THE SEQUENCE ANALYSIS OF THE MITOCHONDRIAL CYTOCHROME OXIDASE I GENE

A MOLECULAR PHYLOGENY OF THE PHLEBOTOMINAE Jérôme Depaquit, Maria Dolores Bargues, Santiago Mas-Coma

Israel Cruz, Rosa Gálvez, Oihane Martín, Ivonne Pamela LlanesAcevedo, Carolina Arcones, Rocío Checa, Ana Montoya, Carmen Chicharro, Silvia Miguelañez, Guadalupe Miró WHO Collaborating Center for Leishmaniasis, Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III. Ctra. Majadahonda-Pozuelo Km2, 28220 Majadahonda-Madrid, SPAIN Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid. Avda. Puerta de Hierro s/n, 28040 Madrid, SPAIN. [email protected]

Keywords: Sandflies, Mediterranean, DNA-barcoding, COI Genomic approaches to taxonomy rely on the variability of DNA sequences to identify organisms. In this sense, these sequences can be considered as genetic barcodes. Mitochondrial genes seem to be the most appropriate candidates for DNA barcoding, among them cytochrome c oxidase I (COI) possess a greater range of phylogenetic signal than any other mitochondrial gene and it has been suggested as the core of a global bioidentification system for animals. DNA barcoding is increasingly used in phlebotomine sandflies taxonomy, using either COI or cytochrome-B (cytb) genes. We aimed to assess the use of COI-based DNA barcoding using sandflies collected from different regions in Spain, for which we sequenced a 658 bp fragment of the COI gene. We completed this data set with DNA sequences available in the GenBank for species prevalent in the Mediterranean region. In total 156 DNA sequences from 4 different phlebotomine sandflies species (Phlebotomus ariasi, P.papatasi, P. perniciosus, and Sergentomyia minuta) from Spain were generated for this work and added to 129 DNA sequences from 14 different phlebotomine species (P. alexandri, P. ariasi, P. bergeroti, P. chadlii, P. longicuspis, P. papatasi, P. perfiliewi, P. perniciosus, P. riouxi, P. sergenti, S. antenata, S. clydei, S. fallax, S. minuta), from 6 different countries (Algeria, India, Italy, Malta, Portugal, Tunisia) obtained from the GenBank. Sequences were aligned and trimmed to get a final alignment of 620 nt. Intraspecific variability was observed in all species but P. bergeroti (a single DNA sequence was available), and haplotypes were defined on the basis of single nucleotide polymorphisms. Distances were estimated with the Jukes Cantor model to confirm the data set was amenable for Neighbor-Joining analysis. With the refined alignment a Neighbor-Joining Tree was obtained using Kimura 2-parameters model of evolution and 2000 bootstrap replicates. All species included in the tree were supported by bootstrap values of 98% or higher, indicating that this approach is a solid candidate for DNA barcoding of phlebotomine sandflies. This work represents a strong contribution to the collection of COI DNA sequences available for phlebotomine sandflies from Spain. We also discuss different approaches to DNA barcoding using other targets, such as cytb, as well as other analysis methods and models of evolution. Acknowledgement: COLCIENCIAS/COLFUTURO 2012, Instituto de Salud Carlos III MPY-1248/12.

Université de Reims Champagne-Ardenne, ANSES, EA4688 – USC. [email protected]

Keywords: Phylogeny, ribosomal DNA, paleobiogeography. Phlebotomine sandflies belong to the subfamily Phlebotominae (Diptera, Psychodidae). The systematics of this group is the inheritance of many typological systematics carried out in the Old World (Abonnenc 1972, Lewis 1982, Artemiev and Neronov 1984, Seccombe, Ready et al. 1993) and in the New World (Young and Duncan 1994 ). However, a few phylogenetical and phenetical studies at the subfamily scale have been carried out (Galati 1995, Rispail and Léger 1998, Rispail and Léger 1998, Galati 2010) based on morphological characters.We propose a molecular phylogeny of the Phlebotominae based on specimens from Africa, Europe, Asia, Oceania and the Americas. Our sampling includes the following genera: Warileya, Spelaeophlebotomus, Idiophlebotomus and Chinius regarding the Hertigiini; Phlebotomus (subgenera Phlebotomus, Paraphlebotomus, Synphlebotomus, Larroussius, Adlerius, Transphlebotomus, Euphlebotomus, Anaphlebotomus, M a d a p h l e b o t o m u s , L e g e ro m y i a , a n d A b o n n e n c i u s ) , Australophlebotomus, Brumptomyia, Oligodontomyia, Sergentomyia (Sergentomyia, Parrotomyia, Sintonius, Rondanomyia, Vattieromyia, Trouilletomyia and some ungrouped species), Grassomyia, Spelaeomyia, Parvidens, Daenemyia, Edentomyia, Micropygomyia (Silvamyia, Sauromyia, Micropygomyia), Sciopemyia, Lutzomyia (Helcocyrtomyia, Castromyia, Tricholateralis, Lutzomyia), Migonemyia (Migonemyia, Blancasmyia), Pintomyia (Pintomyia, Pifanomyia), Dampfomyia (Coromyia), Expapillata, Pressatia, Trichopygomyia, Evandromyia (Aldamyia, Evandromyia, Barrettomyia), Psathyromyia (Foratiniella, Xiphomyia, Psathyromyia), Viannamyia, Martinsmyia, Bichromomyia, Psychodopygus, Nyssomyia and Trichophoromyia regarding the Phlebotominii. We have chosen the following outgroups: Psychoda sp. Sycorax sp., Nemapalpus spp and Bruchomyia sp.We sequenced the complete small subunit of the ribosomal DNA and also several markers of the long subunit (D1, D2 and D8 domains).We compared our results with those previously published based on morphological characters (Galati 1995, Rispail and Léger 1998, Rispail and Léger 1998, Galati 2010). Our data will be of help to discuss the systematics of the Phlebotomine sandflies.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

ID 19-O Taxonomy & Phylogeny

ID 20-O Taxonomy & Phylogeny

DISTRIBUTION, PHYLOGENY AND TAXONOMY OF SERGENTOMYIA IN ETHIOPIA

RAPID IDENTIFICATION OF PHLEBOTOMINE SAND FLIES BY MALDI-TOF MASS SPECTROMETRY

Aysheshm Kassahun1, Kristýna Hlavačková1, Vít Dvořák1, Asrat Hailu2, Alon Warburg3, Jan Votýpka1 and Petr Volf1

Vit Dvořák1, Jérôme Depaquit2, Valentin Pflüger3, Petr Halada4, Kristýna Hlavačková1, Veronique Lehrter2, Jorian Prudhomme5, Sonja Zapata6, Petr Volf1, Alexander Mathis7

1

Charles University in Prague, Czech Republic; 2Addis Ababa University, Ethiopia; Hebrew University of Jerusalem, Israel.

3

[email protected]

Keywords: Sergentomyia, leishmaniasis, S. schwetzi Members of the genus Sergentomyia are dominant sand flies in SubSaharan Africa. Several studies have demonstrated their importance as vectors of reptilian leishmaniasis caused by Sauroleishmania but their role, if any, in the epidemiology and transmission of human leishmaniasis is not well understood. Multiple cases of Sergentomyia feeding on mammals together with their abundance in the foci of human leishmaniasis and occasional records of Leishmania parasites within their bodies detected by microscopy or PCR have led to speculation on Sergentomyia involvement in the epidemiology of leishmaniasis. Recently, however, we experimentally demonstrated that S. schwetzi is refractory to Leishmania parasites infecting humans. To study the biology, species diversity, phylogeny, taxonomic status and distribution of Sergentomyia species in Ethiopia, sand flies have been collected since 2010 using CDC light traps and sticky traps in endemic areas, mainly in domestic and peri-domestic habitats (animal shelters, termite mounds, rodent burrows and caves and acacia and balanite trees). The specimens collected were identified morphologically and by molecular methods, particularly by the sequencing of cytochrome C oxidase I and cytochrome B. Seven Sergentomyia species were found. Males of two morphotypes of S. schwetzi differed in the orientation of the spines on the style, shape of the penis and size of the 6th abdominal tergite. Using laboratoryestablished colonies we investigated the effect of ambient temperature during larval development on the morphological traits of adults. The genetic variability and proteomic profile were assessed by sequencing target genes and analysing of the spectrum produced by MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry), respectively. Moreover, we investigated the possibility of parthenogenesis and autogeny in S. schwetzi . This study was supported by the Bill and Melinda Gates foundation [grant number OPPGH5336] and Grant Agency of Charles University [9108/2014].

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1) Department of Parasitology, Faculty of Science, Charles University in Prague, Prague, Czech Republic. 2) Université de Reims Champagne-Ardenne, ANSES, EA4688 - USC "transmission vectorielle et épidémiosurveillance de maladies parasitaires (VECPAR)", Reims, France. 3) Mabritec SA, Riehen, Switzerland. 4) Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic. 5) MIVEGEC unit, Centre IRD, Montpellier, France. 6) Instituto de Microbiología, University San Francisco de Quito, Ecuador. 7) Swiss National Centre for Vector Entomology, Institute of Parasitology, University of Zurich, Zurich, Switzerland. [email protected]

Keywords: MALDI-TOF, species identification, protein profiling Phlebotomine sand flies are vectors of several pathogens causing diseases in both humans and animals. To elucidate their role in transmission of these pathogens, proper and conclusive species identification is crucial. Since morphological determination is based on minute and sometime dubious characters on their head and genitalia, which may be altered or absent in field-collected material, there is a demand for rapid, simple and cost-effective complementary molecular approaches. Protein profiling using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDITOF MS) was established during the last decade as a routine method in clinical diagnostics of bacteria and fungi. Currently, applications of this method on higher organisms including arthropods are emerging. We demonstrated that MALDI-TOF MS is also feasible for species determination of phlebotomine sand flies. We established a reference database of protein spectra of 20 species from the Old World genera Phlebotomus (subgenera Phlebotomus, Paraphlebotomus, Larroussius, Adlerius and Euphlebotomus) and Sergentomyia as well as from four New World genera (Nyssomyia, Psychodopygus, Psathyromyia and Lutzomyia). Several major vectors of both cutaneous and visceral human leishmaniases are among analyzed species. Protein spectra originated from both sexes of laboratoryreared as well as field collected specimens. Best quality spectra were obtained with frozen specimens, but storage in 70% ethanol is feasible, and specimens stored for up to 25 years could be identified. In addition, we evaluated whether mass spectra obtained on the instruments of the two leading vendors (Bruker Daltonics, Germany; Shimadzu, Japan) can be used for species identification with the software of the other instrument. Such cross-applicability would strongly enhance the value of reference databases established at different institutions. The present study shows that protein profiling by MALDI-TOF MS is well applicable on phlebotomine sand flies and represents an analytical tool which offers high-throughput, sensitive and reliable species identification which is much desired for many applications in vector biology.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

ID 21-O Taxonomy & Phylogeny

ID 22-O Taxonomy & Phylogeny

TWO NEW SPECIES OF THE SUBGENUS TRANSPHLEBOTOMUS REVEALED BY MOLECULAR TAXONOMY

DNA SEQUENCE ANALYSIS SUGGESTS THAT CYTBND1 PCR-RFLP MAY NOT BE APPLICABLE TO S A N D F LY S P E C I E S I D E N T I F I C AT I O N THROUGHOUT THE MEDITERRANEAN REGION

Vit Dvorak1, Ozge Erisoz Kasap2, Jerome Depaquit3, Bulent Alten2, Jan Votypka1, Petr Volf1 1

Department of Parasitology, Charles University in Prague, Prague, Czech Republic. 2 Department of Biology, Hacettepe University, Ankara, Turkey. 3Laboratoire de Parasitologie, Université de Reims, Ardenne, France. [email protected]

Keywords: Transphlebotomus, molecular taxonomy, new species The subgenus Transphlebotomus comprises only three described species (P. mascittii, P. canaaniticus, P. economidesi) with markedly different geographical distribution. While P. mascittii is widely present in the north of the Mediterranean basin and recently recorded in Algeria, and stretches up to the northern limits of sand fly presence in Europe in localities in Belgium, Austria and Germany, P. canaaniticus and P. economidesi have more restricted distribution. Due to their similar morphology, proper identification remains difficult and relies mainly on molecular markers. This subgenus was long time neglected. The biology of its species is poorly understood, they are little represented in field collections as some of them are expected to be cavernicolous and are thought not to be incriminated in Leishmania parasites transmission, although closely related subgenera Larroussius and Adlerius harbor several proven vectors of both cutaneous and visceral leishmaniasis. However, presence of P. mascittii in several foci of autochtonous canine leishmaniasis in southern Germany suggests that Transphlebotomus species may be a vector under certain circumstances. We studied Transphlebotomus species from Crete, the largest island of the Aegean Archipelago and from a locality on south-western coast of Anatolia, approximately 500 km away from Crete. To clarify the taxonomic position of the specimens studied, P. mascittii from Belgium, Germany and France, P. economidesi from Cyprus and P. canaaniticus from Lebanon were used as reference sequences. Based on the sequencing analysis of several genes (cytochrome B, NADH dehydrogenase subunit 4, cytochrome oxidase I) two new putative species of this subgenus were found, both of them characterised also by following morphological characters. One species is described from males only and is mainly individualised by long third antennal segments coupled with a low number (13>-20) of coxal setae. The second one is described from both males and females. The latter are remarkable by their pharyngeal armature which is Adlerius-like more than Transphlebotomus-like. Moreover, P. economidesi was found in Turkey sympatrically with these two species, which is the first record of this species outside Cyprus. Our findings indicate that the taxonomy of the subgenus Transphlebotomus should be thoroughly studied by molecular and morphological methods together with investigations concerning possible vectorial capacity in Leishmania transmission.

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Ivonne Pamela Llanes-Acevedo, Carolina Arcones, Rosa Gálvez, Oihane Martín, Rocío Checa, Ana Montoya, Carmen Chicharro, Silvia Miguelañez, Susana Cruz, Guadalupe Miró, Israel Cruz WHO Collaborating Center for Leishmaniasis, Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III. Ctra. Majadahonda-Pozuelo Km2, 28220 Majadahonda-Madrid, SPAIN Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid. Avda. Puerta de Hierro s/n, 28040 Madrid, SPAIN. [email protected]

Keywords: Mediterranean, sandflies, species, cytb-nd1 Molecular methods are increasingly used for both species identification of sandflies and assessment of their population structure. In general they are based on DNA sequence analysis of targets previously amplified by polymerase chain reaction (PCR). However, this approach requires access to DNA sequence facilities and in some circumstances is time consuming. Though DNA sequencing provides the highest degree of information, other downstream applications of PCR are explored to help in species identification., It has thus been proposed that the amplification of a DNA region encompassing partially both the cytochrome-B (cytb) and the NADH dehydrogenase 1 (nd1) genes, and further digestion with the restriction enzyme AseI allows for the rapid identification of the most prevalent species of phlebotomine sandflies in the Mediterranean region based on their restriction fragment length polymorphism (RFLP). We sought to validate this methodology using sandflies collected from different regions in Spain, for which we amplified the cytb-nd1 target and obtained its DNA sequence. We completed this data set with DNA sequences available in the GenBank for species prevalent in the Mediterranean region. In all, 155 DNA sequences from 4 different phlebotomine sandfly species (Phlebotomus ariasi, P.papatasi, P. perniciosus, and Sergentomyia minuta) from Spain were generated for this work and added to 146 DNA sequences from 9 different phlebotomine species (P. ariasi, P. chabaudi, P. neglectus, P. papatasi, P. perfiliewi, P. perniciosus, P. riouxi, P. sergenti and S. minuta), from 18 different countries (Afghanistan, Algeria, Cyprus, Egypt, France, Iran, Israel, Italy, Jordan, Kosovo, Malta, Morocco, Palestine, Portugal, Spain, Syria, Tunisia, Turkey) obtained from the GenBank. In silico analysis revealed that this PCR-RFLP method does not provide a unique and specific profile for each species tested, intraspecific variability occurs in some of them (P. ariasi, P. papatasi, P. perniciosus and S. minuta), and there are patterns which are quite similar between different species (eg. P. chabaudi, P. riouxi, and P. sergenti show a RFLP pattern similar to intraspecific variants of P. ariasi and P. perniciosus). In our sample the results obtained by in silico analysis were further bench tested for confirmation. Though this method has previously been shown to be useful in Italy, it may not be applicable throughout theMediterranean region. Acknowledgement: COLCIENCIAS/COLFUTURO 2012.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

ID 23-O Taxonomy & Phylogeny

ID 24-O Taxonomy & Phylogeny

MULTILOCUS REAL TIME PCR IDENTIFICATION OF PHLEBOTOMINE SAND FLIES CAPTURED IN SOUTHERN ITALY

THE GENUS IDIOPHLEBOTOMUS (DIPTERA; PSYCHODIDAE)

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Nicole Léger and Jérôme Depaquit Reale S, Torina S, Zaffora G, Cosenza M, Pitti R, D'Agostino R, Intile S, Piazza M, and Vitale F. Istituto zooprofilattico Sperimentale della Sicilia A. Mirri. Palermo Italy.

Faculté de Pharmacie de Reims France. [email protected]

[email protected]

Keywords: Real Time PCR, Phlebotominae, Leishmania, Diptera Phlebotomine sand flies (Diptera: Psychodidae) living in Mediterranean area, are small-sized blood-sucking insects feeding on a wide range of hosts. They can be vectors of pathogens responsible for human and animal diseases as leishmaniasis. Out of over 800 sand fly species that have been described to date, approximately 10% are proven or suspected vectors of bacteria (e.g., Bartonella bacilliformis), viruses (e.g., Phlebovirus, Vesiculovirus) as well as protozoans from the genus Leishmania.. The identification of phlebotomine sand flies at species level relies on morphology of key structures including pharynx, spermathecae and cibarium for females and genitalia for males. Nonetheless, the morphological identification requires taxonomic expertise and it is time-consuming This study reports a combined analysis of ribosomal DNA target in phlebotomine sand flies from the Sicilian Mediterranean region. A 100 bp long fragments of DNA encompassing a part of ITS2 ribosomal region were amplified in Real Time test for Phlebotomus perniciosus, Phlebotomus perfiliewi, Phlebotomus neglectus, Phlebotomus papatasi, and Sergentomyia minuta, captured at various sites of southern Italy. Based on high interspecific rDNA difference, a universal couple of primers and 5 different probes were employed to reveal the percentage of the different sand fly species in the captures. Two different PCR mixtures were optimized to detect the cluster constituted by P. neglectus, P. perfiliewi, P. perniciosus, and the cluster P. papatasi, S. minuta respectively. On the basis of the obtained results, we conclude that the test can be used as preliminary approach to identify the species in captured pool of insects, and to individualize those that are considerably rare. This study demonstrates the utility of a multilocus approach to provide a tool for the molecular identification of the most prevalent phlebotomine sand flies in southern Europe. Obtained data showed the average percentage for each species in the catch, indicating the power to resolve the species mixture collected in the traps used in Leishmania endemic areas.

All the species (16 or 15 ?) of Idiophlebotomus are localized inside the oriento-australasian area. This genera is the sister group of the African Spelaeophlebotomus , that is to say that they have a gondwanian origin and attests of an insular evolution of the phlebotomine sandflies during the long travel of the future India from Africa to Asia. Their morphological study is a key for the phylogeny of the Hertiginii tribe.

ID 25-O Ecology & Epidemiology SAND FLY SPECIES TRANSMITTING VISCERAL LEISHMANIASIS AT THE SEMIARID REGION OF VENEZUELA Añez Néstor, Rojas Agustina, Yépez José Yancarlos Universidad de Los Andes, Mérida, Venezuela; Universidad Experimental Francisco de Miranda, Coro, Venezuela. [email protected]

Keywords: Sand fly-species, Visceral-leishmaniasis, Semiarid, Venezuela A total of 12 sand fly species were recognized from 6000 specimens collected during a study carried out in 12 different localities at the semiarid region of western Venezuela where visceral leishmaniasis (VL) is endemic. From the total identified specimens, 81% (N=4892) belong to Lutzomyia longipalpis (15%; N=898) and Lu.evansi (66%; N=3994). The former, a species incriminated as vector of VL in most Latin-American countries, and Lu.evansi considered a potential vector of Leishmania infantum in VL endemic areas from Colombia and Venezuela. The study also revealed that Lu.evansi was present in all the 12 sampled localities (100%) while Lu.longipalpis was collected in 5 of them (41%). In addition, a PCR assay randomly performed to detect L.infantum from sand fly batches, collected in a village where active VL cases were detected, revealed infection in 3 of 5 (60%) and 4 of 18 (22%) batches of Lu.evansi and Lu.longipalpis, respectively. Statistical analysis using the Fisher Exact Test revealed no significant difference (Pvalue = 0.142) when results for Leishmania-infection in batches of both sand fly species were compared. These results suggest that despite the observed abundance and distribution of Lu.evansi over Lu.longipalpis, both sand fly species are sympatrically transmitting VL in the semiarid region of western Venezuela.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

ID 26-O Ecology & Epidemiology

ID 27-O Ecology & Epidemiology

NATURAL BREEDING SITES OF LUTZOMYIA EVANSI (DIPTERA: PSYCHODIDAE) IN THE URBAN ZONE OF OVEJAS, SUCRE - COLOMBIA

LUTZOMYIA FAUNAS (DIPTERA: PSYCHODIDAE) IN THE EAST SIDE OF THE ANDEAN CORDILLERA IN CUZCO, PERU

Luis G. Estrada2, Horacio Cadena1, Edgar Ortega2, Luz A Acosta1, Andrés Vélez-Mira1, Rafael J Vivero1, Eduar E Bejarano2, Sandra Uribe3, Iván D Vélez1

Perez JE1, D Rado2, D Quispe2, A Quispe2, F Toccas2, M Chacon2, B Valencia1, A Llanos1, M Quispe2, E Aguilar2, R Pacheco2 & E Ogusuku3

1

PECET (Programa de Estudio y Control de Enfermedades Tropicales) - Universidad de Antioquia, Medellín, Colombia; 2Grupo de Investigaciones Biomédicas, Universidad de Sucre, Sincelejo, Colombia; 3Grupo de Investigación en Sistemática Molecular, Universidad Nacional, Medellín, Colombia.

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1

Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia. 2Instituto Universitario de Enfermedades Tropicales y Biomedicina del Cuzco. Universidad Nacional San Antonio Abad de Cuzco, Peru. 3Direccion General de Salud Ambiental, Ministerio de Salud. Lima, Peru. [email protected]

[email protected]

Keywords: Peru, Lutzomyia, faunas, Cuzco Keywords: Immature, Phlebotomine, Leishmaniasis, Natural breeding The sand flies natural breeding places, despite their potential to assist the biological control, are poorly understood so far, due to the difficulties of isolation of immatures stages from the soil where they occur. The aim of this study was to identify natural breeding sites of Lutzomyia evansi in three zones of peri-urban area of the municipality of Ovejas, Sucre – Colombia. This region shows a bimodal climate: a rainy period from May to November and a dry period from December to April. The annual precipitation and mean temperature is 1380 mm and 28°C, respectively, and according to Holdridge the ecological lifezone is Tropical Dry Forest. The study was conducted between March 2013 and February 2014. Each zone consisted of 50 houses and search of breeding was carried out in the peri and intradomicile of these homes. Soil samples were taken around the bases of trees, leaf litter, hollow trees and soil under fallen trees. A total of approximately 503 kg of soil and associated organic matter were processed. Two different techniques: direct-observation and recovering emerging adults (incubation cages), were used to verify the presence of immature sand flies. During the rainy season the largest number of immature sand flies was recovered mainly in the base of trees. 70 natural breeding sites positive were identified and distributed as follows: zone1 n = 25; Zone 2 n =23; zone 3 n = 22. A total of 230 immature forms were found, which 135 (118 larvae and pupae 17) by direct-observation while 95 adults emerged by soil incubation. These results showed a richness of 6 species where Lutzomyia evansi was represented by 118 (51%) followed by Lu. rangeliana with 10 (4%), Lu. cayennensis cayennensis 7 (3%), Lu. micropyiga 6 (3%), Lu. atroclavata 4 (2%), and Lu. gomezi with 1 (0.4%). The 55.71% positive natural breeding sites were Lu. evansi, associated to the base of a shrub called locally as Uvito (Cordia dentata). 128 immature forms (including 44 exuviae recovered) that did not reach adulthood will be identified based on mitochondrial COI gene. This is the first record of peri-urban breeding sites of Lu. evansi in Colombia. The knowledge about natural breeding places for sand flies and vector density may represent useful information for directing efforts at biological control and consequently control the incidence of disease.

Lutzomyia sandflies are wide distributed in the East side slopes of the Andean Cordillera of Cuzco Department. The highland vegetation is dominated by grasses and shrubs, the arboreal vegetation is mainly the introduced Eucalyptus spp. The lower lands are dominated by natural tropial forest. The whole area is endemic for bartonellosis (verruga peruana or Carrion disease) and leishmaniosis (uta).Objective: To determine the anthropophilic Lutzomyia fauna of the study area. Sandflies were collected with Shannon traps with protected human as attractant from 18:00 to 23:00 in the localities mentioned below. The Lutzomyia faunal composition is notoriously affected by the altitude, there is one species (L. (Helcocyrtomyia) peruensis) in Calca, Urubamba, Pisac, Ollantaytambo at around 3000 m of altitude; in San Pedro at 1380 m 5 species were reported (L. (H.) guderiani 74.9 %). These situations contrast with the reports for the lower lands, 26 species in Pillcopata at 1000 m (L. (Psychodopygus) c. carrerai 53.6 %); 37 species in Aguas Calientes at 890 m (L. (Nyssomyia) y. yuilli 57%); 34 species in Alto Ivochote at 880 m ((L. (Nyssomyia) y. yuilli 53%); and 29 species in Paratori at 450 m (L.(P.) llanosmartinsi 72.5 %). Lutzomyia (Helcocyrtomyia) species predominate in the highest altitudes (>3000m) along the Andean Cordillera in Peru, this is the case of L. peruensis in Cuzco. The west side of the Cordillera is dry and arboreal vegetation is restricted to protected areas in the bottom of some valleys. Lutzomyia fauna in this area is 3 to 6 species, Lutzomyia peruensis and L verrucarum are the most common species, both are vectors of bartonellosis and Andean leishmaniosis (uta).

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

ID 28-O Ecology & Epidemiology

ID 29-O Ecology & Epidemiology

DIFFERENTIAL EXPOSURE TO SAND FLY BITES IN SAUDI ARABIA

PREDICTING THE GEOGRAPHICAL DISTRIBUTION OF LEISHMANIASIS VECTOR SPECIES IN CENTRAL WEST BRAZIL

Karina Mondragon-Shem1; Waleed Alsalem1,2; Louise Kelly-Hope1,3; Maha Abdeladhim4; Mohamed Alzahrani2; Ziad Memish2; Jesus Valenzuela4; Alvaro Acosta-Serrano1, 5 1

Department of Parasitology, Liverpool School of Tropical Medicine, England, UK; Saudi Ministry of Health, Riyadh, Kingdom of Saudi Arabia; 3Centre for Neglected Tropical Diseases, Liverpool School of Tropical Medicine, England, UK; 4Vector Molecular Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, United States of America; 5 Department of Vector Biology, Liverpool School of Tropical Medicine, England, UK. 2

[email protected]

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Paulo Silva de Almeida, Alan Sciamarelli, Josué Raizer, Jaqueline Aparecida Menegatti, Sandra Cristina Negreli Moreira Hermes, Maria do Socorro Laurentino de Carvalho, Andrey José de Andrade, Rodrigo Gurgel-Gonçalves Universidade de Brasília. [email protected]

Keywords: Phlebotominae, ecologic niche modeling, Central West Brazil, surveillance.

Keywords: Phlebotomus papatasi, biting exposure. Human antibody response to sandfly saliva can serve as a marker of exposure to the bite of vector species, and is valuable to assess risk of disease in different endemic settings. In Saudi Arabia, zoonotic cutaneous leishmaniasis (ZCL) is caused by Leishmania major and transmitted by Phlebotomus papatasi, while anthroponotic CL (ACL) is caused by L. tropica and vectored by Ph. sergenti. Salivary protein SP32 of Ph. papatasi, the main target of the human immune response against the saliva of this species, is an ideal biomarker to measure exposure. The recombinant form of this protein, rPpSP32, allows for mass screening of sera in endemic areas. In this work, we determined the level of exposure to Ph. papatasi bites in individuals from CL endemic areas in Saudi Arabia. We obtained sera from healthy residents CL as well as patients with active and cured infection, from CL foci in Al-Ahsa, Al-Madinah and Asir. We measured the levels of anti-SP32 antibodies by ELISA using rPpSP32. Additionally, we collected sandflies from the same regions to identify the vector species present the foci. rPpSP32 was successfully recognized by human sera from the ZCL endemic areas in Saudi Arabia. We found higher titres of anti-SP32 antibodies in CL patients than in healthy and CL-cured individuals in Al-Hasa, while in Al-Madinah there was no difference between the groups. In both of these ZCL foci Ph. papatasi was the predominant sand fly species. Interestingly, we also detected a significant difference between the levels of anti-SP32 antibodies in patients with papular and nodular lesion types in Al-Madinah. In Asir, where Ph. sergenti was the most common species, we found low levels of anti-SP32 antibodies in ACL patients. This revealed a potential cross-reaction with the Ph. sergenti SP44 PpSP32-like salivary protein, which was supported by the high similarity with the SP32 protein sequence. Additionally, we discovered the non-local workforce in Al Ahsa exhibited higher antibody levels and increased lesion numbers compared to long-term residents, which will have important implications for leishmaniasis control programmes in Saudi Arabia. This is the first study using rPpSP32 protein to assess biting exposure in CL patients. Cross-reaction with Ph. sergenti salivary proteins must be kept in mind when evaluating exposure in areas where both species co-exist. Our results suggest a correlation between biting exposure, disease outcome and clinical presentation.

Central West Region of Brazil (CWB) comprises the states of Mato Grosso (MT), Mato Grosso do Sul (MS), Goiás (GO) and the Federal District (FD). The present study analyzed the geographical distribution of the main sand fly species associated with leishmaniasis in CWB and climatic factors associated with its occurrence. Distributional data of seven sand fly species were obtained by the entomology laboratories of “Secretarias Estaduais de Saúde”, museums and literature review between 1978 and 2014. Data were separated into two sets: one for model calibration (75% of points) and one for model evaluation (25% of points). Climatic data covered areas of CWB, resample to a spatial resolution of 5 km. Models were produced based on eight climatic variables using Maxent. Median output grids were used as a hypothesis of suitability, and they were imported into ArcView and DIVA for analysis. Model accuracy was assessed by examining omission rate associated with test points. Maxen's jackknife test was used to identify variables that most influenced models. In total 566 records were analyzed. Bichromomyia flaviscutellata (n=61), Lutzomyia longipalpis (n=128) and Nyssomyia whitmani (n=200) had wide distribution in CWB. Lu. cruzi occurred in 52 municipalities in southwestern and eastern regions of MT, northern MS and southern GO. Ny. antunesi showed more restricted distribution in the state of MT and north of MS (n=71). Ny. intermedia (n=33) and Ny. neivai (n=21) were distributed in the states of GO, MT and FD. Predictive models indicated areas with high climatic suitability for Ny. whitmani (southern and northern regions of MT and MS, central GO), Lu. longipalpis (southwestern MS and northern MT), Lu. cruzi (southeastern MT, southwestern GO and northern MS), Bi. flaviscutellata (northern MT), Ny. intermedia (central GO and southwestern MT), Ny. neivai (southern, southwestern and eastern MS), and Ny. antunesi (central MT and eastern GO). The models showed < 1% omission error. The variable that most influenced models was the temperature in the coldest months. Moreover, the occurrence of Lu. longipalpis was associated with dry areas, unlike the models of the other species that were strongly influenced by the annual precipitation. The results demonstrated different patterns of geographical distributions for leishmaniasis vectors in the CWB, which can assist surveillance and control strategies of this disease.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

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ID 30-O Ecology & Epidemiology

ID 31-O Ecology & Epidemiology

ECOLOGY OF SANDFLIES IN RISK ZONES FOR TEGUMENTARY LEISHMANIASIS TRANSMISSION IN SANTARÉM MUNICIPALITY, WESTERN PARÁ STATE, BRAZIL

PHLEBOTOMUS ORIENTALIS AND THE FALL AND RISE IN VISCERAL LEISHMANIASIS IN EAST AFRICA Dia-Eldin A Elnaiem

Raquel Gonçalves, Daniela Cristina Soares, Ricardo Guimarães, Walter Souza Santos, Gilberto Cesar de Sousa, Anadeiva Portela Chagas, Lourdes Maria Garcez

University of Maryland Eastern Shore. [email protected]

Instituto Evandro Chagas/SVS/MS and Universidade do Estado do Pará. [email protected]

Keywords: Phlebotmus orientalis , East Africa

Keywords: Ecology, sand flies, leishmaniasis, risk zone

Phlebotomus orientalis sand fly is the main vector of Leishmania donovani in most foci of visceral leishmaniasis (VL, kala azar) in East Africa. The two other vectors, P. martini and P. celiae, are responsible of transmission of L. donovani in limited foci in the southeastern region of Ethiopia and the border areas of the Republic of South Sudan (RSS) and Kenya. Due to marked fluctuations in the abundance of P. orientalis, East African visceral leishmaniasis is characterized by periodic outbreaks that result in high morbidity and mortality rates. The severity of these outbreaks is exacerbated by lack of knowledge that allows their prediction both in time and space. Our observations in several areas in Sudan indicate that this vector may disappear for several years and then erupt in large numbers. While we believe the cyclical nature of VL and its vector is most likely related to climatic conditions, little is known about the specific environmental factors responsible for the epidemics. This is partly due to lack of reliable surveillance system that provide accurate estimates of number of cases and abundance of the vector. We recently embarked on a multicenter research initiative to elucidate the environmental determinants affecting the fall and rise of VL and use them to construct a model that can help predict future outbreaks. We correlated numbers of VL cases with climatic data in eastern Sudan and RSS. Our results showed that the incidence of the disease is negatively correlated with the rainfall at the end of the dry season. These findings correspond closely with our observations on the ecology of P. orientalis. Future studies will attempt to build Early Warning System for Visceral Leishmaniasis, based on climate and other environmental factors.

Tegumentary leishmaniasis (TL) in Brazil used to be near 10 times more incident than visceral leishmaniasis (VL). The environmental and socio-economic context influence both in the different endemic areas. Pará is among the five Brazilian states with the highest incidence rates of TL over the last years. The municipality of Santarém is one of the main centres reporting new cases of the disease. We studied the ecology of sand flies in two spatial circuits (SCs) of TL in Santarém using the program PAST v.2.03 (PAlentological STatistic) for calculating ecological indexes: Shannon (H'), Pielou (J) and Dominance (D). To define the SCs we used geographical coordinates from transmission and housing locals of patients and the Kernel density estimator. Thirty-one records selected of a database with 102 TL patients reported in 2010 and 2011 were included. The patients lived in plateau areas from Santarém, had one or more primary cutaneous/mucocutaneous lesion and the TL confirmed by parasitological and molecular diagnosis. Among the etiologic agents infecting the patients there were Leishmania (Viannia) braziliensis, L. (V.) shawi, L. (V.) lainsoni, L. (V.) naiffi and one hybrid form of L. (V.) braziliensis/guyanensis. We identified two SCs of TL in images classified according the deforestation (2002-2009) and the land uses (Basin Restoration Program, PRODES – TerraClass Project; National Institute of Space Research, INPE, Brazil, 2011): Jatobá and CortaCorda. There were captured 417 sand flies (24 species) throughout two entomological surveys using Shannon and CDC traps. Most were females (312; 75%) and the sand flies number did not vary in wet (226/417; 54%) and dry (191/417; 46%) seasons. Nine genus were identified: Bichromomyia, Evandromyia, Lutzomyia, Micropygomyia, Nyssomyia, Psathyromyia, Psychodopygus, Sciopemyia, Trichopygomyia. The ecological indexes varied (H'=0.5-1.72; J= 0.44-0.78; D=0.46-0.91) and confirmed high diversity and the importance of the vectors Lutzomyia longipalpis (VL) and Psychodopygus complexus (TL) in Jatobá (urban-rural and ecotourism area) and Psychodopygus davisi (TL) in Corta-Corda (rural farm, around a hydroelectric dam). There were less important the vectors Nyssomyia antunesi, Bichromomyia flaviscutellata and Nyssomyia anduzei (TL). The results support the Leishmania species diversity in the patients living in the SCs of TL, representing the importance of such analysis in entomological surveys.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

ID 32-O Ecology & Epidemiology

ID 33-O Ecology & Epidemiology

DISTRIBUTION OF LEISHMANIASIS VECTORS IN THE MEDITERRANEAN BASIN IN THE LIGHT OF CLIMATE CHANGE

CHARACTERIZATION OF BREEDING SITES OF PHLEBOTOMINE SAND FLIES IN THE JUDEAN DESERT AND NORTH-WEST ETHIOPIA

Bilel Chalghaf, Amine Toumi, Sadok Chlif, Jihene Bettaieb, Mariem Harrabi, GozeBertin Bénié, Afif Ben Salah

Aviad Moncaz, Oscar Kirstein, Araya Gebresellassie, Wossenseged Lemma, Solomon Yared, Teshome Gebre-Michael, Asrat Hailu, Moshe Shenker and Alon Warburg

Institut Pasteur Tunis, Universite de Sherbrooke. [email protected]

Keywords: climate change, ecological niche, modeling, sand flies Due to climate change, the geographical distribution of sandflies during the last decades has shifted northward from latitudes below 45○N in south Europe (Martens and McMichael 2002) to latitudes just above 50○N (Naucke et al., 2008). Recent studies show that some phlebotomine sandflies were recorded in several parts of Germany and Belgium (Naucke et al., 2008, Mencke; 2011). In Central Europe, some autochthone leishmaniasis cases are being recorded in regions traditionally regarded as leishmaniasis-free countries (Gogoaşe et al., 2013, Tánczos et al., 2012).In this study we attempted to predict current distribution of six leishmaniasis vectors in the Mediterranean basin and forecast species geographical shift under future climate scenarios using ensemble ecological niche modeling approach. Species records were obtained from scientific surveys published in the research literature between 2006 and 2012. A series of climate metrics, describing temperature and precipitation in the study area under two climatic scenarios, were obtained from WorldClim database. We used six individual models contributing to the consensus model including two regression techniques (Generalized Linear Models, GLM; Generalized Additive Models, GAM), two machine learning techniques (Artificial Neural Networks, ANNs; Maximum Entropy, MaxEnt) and two classification techniques (Surface Range Envelop, SRE; Random Forest, RF) (why two models from each category of models?). Alternative formulation: A consensus model was derived from six varieties of modeling approaches (regression, machine learning and classification techniques) in order to ensure valid prediction of distribution of vectors under different climate scenarios. Model performance was generally high for the included species with a specificity (True Negative Rate) ranging from 81.03% to 96.52% (mean=86.94%) and a sensitivity (True Positive Rate) ranging from 87.93% to 100% (mean=96.98%). Our work evidenced the hypothesis of the wide spread of Leishamania vectors under climate change scenarios. All the studied species are prospected to gain new areas that are actually not suitable for vectors' survival. Phlebotomine sandflies are prospected to invade extra-Mediterranean regions especially West.

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The Kuvin Center for the Study of Infectious and Tropical Diseases, Department of Microbiology and Molecular Genetics, The Institute of Medical Research IsraelCanada, Faculty of Medicine, The Hebrew University of Jerusalem, Hadassah Medical School, Jerusalem, Israel. [email protected]

Keywords: Phlebotomus vertisols caves trees Identification and characterization of breeding sites is essential for the implementation of source-reduction for the control of sand flies and leishmaniasis. Sand fly breeding sites were identified and characterized in two foci of leishmaniasis: In the Judean Desert where the dominant sand fly species that transmits Leishmania tropica is Phlebotomus sergenti, and in NW Ethiopia where L. donovani is transmitted by Ph. orientalis. Sand flies emerging from their breeding sites were trapped by emergence traps (ETs) for 2–8 consecutive nights. In the Judean desert, Ph. sergenti were trapped emerging from caves and artificial support walls made of large rocks. . Young (recently eclosed) male sand flies were found in both habitats. The temperatures and relative humidity inside caves were stable and soil samples collected in the cave opening were rich in organic matter. In Ethiopia Ph. orientalis breeding sites were identified in cracks in vertisols including fallow fields, dry riverbed/bank and near trees. No Phlebotomus spp. were trapped in sandy clay loam soil. More Ph. orientalis were trapped near tree trunks than in open fields but the difference was insignificant. Large numbers of Sergentomyia spp. were trapped along with Ph. orientalis mostly outnumbering Phlebotomus spp. by an order of magnitude. Initial microclimatic monitoring in the two habitats was conducted at the end of rainy season, and showed stable subterranean temperatures and RH. Conditions remained stable through early dry season close to trees but in open fields temperature fluctuated by 5-6ºC and RH decreased significantly. Hypoxic conditions evolved during in tree root systems during the rainy season but oxygen levels rebounded to atmospheric values once cracks reformed during the dry season. Organic matter content was high (>5%) in both habitats. Soil pH was moderately alkaline and electrical conductivity was low in both habitats and depths. Low oxygen concentrations in soil close to tree root systems during the rainy season may be caused by microbial metabolism. Decomposing organic matter, stable temperatures and elevated soil moisture maintained throughout dry season near the trees at shallow and deep horizons, and in the open field at deep horizon, probably maintain suitable conditions for sand fly larvae.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

ID 34-O Ecology & Epidemiology

ID 35-O Ecology & Epidemiology

F I N E S C A L E A N A LY S E S O F FA C T O R S CONTRIBUTING TO THE TRANSMISSION OF VISCERAL LEISHMANIASIS IN NORTH ETHIOPIAN VILLAGES: AN ECO-EPIDEMIOLOGICAL APPROACH

SERO-PREVALENCE L. DONOVANI IN LABOUR MIGRANTS POPULATION AND ENTOMOLOGICAL RISK FACTORS IN EXTRA-DOMESTIC HABITATS OF METEMA-HUMERA LOWLANDS - KALA-AZAR ENDEMIC AREAS IN THE NORTHWEST ETHIOPIA

Oscar David Kirstein, Araya Gebreselassie, Aviad Moncaz, Asrat Hailu, Teshome Gebre-Michael and Alon Warburg

Wossenseged Lemma1,2, Habte Tekie2, Meshesha Balkew3, Teshome Gebre-Michael3, Alon Warburg4, Asrat Hailu5

Department of Microbiology & Molecular Genetics, The Institute for Medical Research Israel-Canada, The Kuvin Centre for the Study of Infectious &Tropical Diseases, The Hebrew University – Hadassah Medical School, The Hebrew University of Jerusalem. [email protected]

Keywords: Sand fly, Kala Azar, Epidemiology, Visceral leishmaniasis Landscape ecology and landscape epidemiology comprise a set of tools used for spatial and temporal analyses of vector-borne and other diseases. In Sudan and Northern Ethiopia, visceral leishmaniasis (VL) caused by Leishmania donovani, is transmitted by Phlebotomus orientalis. The epidemiology of VL is the result of a complex matrix of interactions between the vectors, the pathogens and the hosts with the environment. This study aims to characterize the risk factors for contracting VL by gaining a profound understanding of the factors contributing to its transmission to humans. During 2010 - 2013 a transverse cohort study was conducted, based on a census comprising over 11,000 individuals in 18 villages in the Tahtay Adiabo district of northern Ethiopia. Around 4,900 individuals were screened and tested for infection with L. donovani. Based on the incidence of VL reported during the census, the Leishmania infection rates determined by PCR during the cohort samplings and the environmental and geographical variables, three villages were selected for an in-depth ecoepidemiological study. In 2014 a nested case-cohort study of ~400 individuals was conducted in the three selected villages, confounder information available in the previous cohort data-base was used to select controls that closely match cases in term of sex, age and geographical location. By analyzing satellite images taken during the dry season, it was possible to identify that the majority of villages affected by VL during recent years are inside an area characterized by vertisols (radius 28 Km). Remote sensing analysis showed high spectral variability produced by a mix of vertisols, sandstone outcrops, other sedimentary rocks and varied vegetation land cover. Entomological studies identified that P. orientalis is closely associated with vertisol ecosystems rather than specific micro-environments, being most abundant in cracked black cotton soils. Spatial analysis of the incidence of VL, show a clustered distribution within populated areas and micro-clusters of more than one case per house or nearby house. Houses of patients were most frequently within a few meters of vertisol fields. These observations may support the hypothesis that the infection with L. donovani occurs in domestic environments rather than in the field. The current study is ongoing and more data analysis will validate or otherwise affect the results presented here.

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1 Department of Parasitology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia. 2 Department of zoology, Faculty of Life Science, Addis Ababa University, Addis Ababa, Ethiopia. 3Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia. 4Department of Microbiology & Molecular Genetics, The Institute for Medical Research Israel-Canada, The Kuvin Centre for the Study of Infectious & Tropical Diseases, The Hebrew University – Hadassah Medical School, The Hebrew University of Jerusalem, 91120, Israel. 5Department of Microbiology, Immunology & Parasitology, Faculty of Medicine, Addis Ababa University, Addis Ababa, Ethiopia. [email protected]

Keywords: kala-azar infection, labour migrants, risk factors; Ethiopia Visceral leishmaniasis (VL) or kala-azar is a systemic disease transmitted by phlebotomine sand flies and almost always fatal if left untreated. The aim this study was to evaluate sero prevalence of kalaazar infection and associated risk factors which affect the incidence of kala-azar. Study on seasonal population dynamics of P.orientalis were conducted in different villages of Humera before blood samples was randomly obtained from labour migrants involved in harvest of sesame for Direct Agglutination Test (DAT) after oral consents were obtained during October 27 to November 7, 2013. A structured questionnaire was used to obtain demographic information and factors that affect the incidence of kala-azar infection in labour migrants. About 80% of the labour migrants were aged from 15 to 34 years and came from Amhara (75.2%), Tigray (21.2%) and other parts of Ethiopia (3.6%). Stayed both harvest and weed season in Humera, Involved in night harvest, Sleep in the farm under Balanites aegiptica and Knowledge about, at least, a sign or a symptom of kala-azar were the major factors (P< 0.05) influencing kala-azar infection (DAT positivity or sero reaction). The total P. orientalis of 7022 (3277 female and 3745 male) were collected from agricultural fields and thickets in A. seyal in Adebay, Rawyal, Gelanzeraf and Mysegen – Mehari using 932 CDC traps. The month with the highest mean P. orientalis/CDC (20.47) abundance was in April. The mean P. orientalis/CDC during dry season (Nov - April) was highest (Mean ± Std D =7.33 ± 18.11) compared to May – August weeding (2.05 ± 4.47) and September – October sesame harvest (0.41± 0.77) seasons. Kala-azar infection in labour migrants seemed to relate with P. orientalis bite during May – August weeding season compared to September – October least P. orientalis abundance harvest season. Peak Kala-azar incidence in permanently settled farmers in villages of Shiraro also seemed related to P. orientalis bite during May – Augest weeding season which took 2- 6 months before the peak manifestations of the disease in January. Labour migrants are exposed to kala-azar infection during May - August clearing farm and weeding season. But, stay during September – October harvest season or involve in night harvest were not increase the risk of kala-azar infection as P. orientalis population drops to almost to zero.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

40

ID 36-O Ecology & Epidemiology

ID 37-O Ecology & Epidemiology

HOST PREFERENCE PATTERNS OF PHLEBOTOMUS (LARROUSSIUS) ORIENTALIS PARROT, 1936 (DIPTERA: PSYCHODIDAE) IN THE VISCERAL LEISHMANIASIS ENDEMIC AREA OF TAHTAY ADIYABO DISTRICT, NORTHERN ETHIOPIA

BIONOMICS OF PHLEBOTOMINE SANDFLIES IN A HIGHLAND KALA-AZAR FOCUS IN LIBO-KEMKEM DISTRICT, NORTHWESTERN ETHIOPIA Esayas Aklilu, Habte Tekie, Meshesha Balkew, Alon Warburg, Asrat Hailu, Teshome Gebre-Michael

Araya Gebresilassie1, Ibrahim Abbasi2, Oscar David Kirstein2, Aviad Moncaz2, Habte Tekie1, Meshesha Balkew3, Alon Warburg2, Asrat Hailu4, and Teshome Gebre-Michael3

Addis Ababa University. [email protected]

1

Department of Zoological Sciences, Addis Ababa University, Addis Ababa, Ethiopia, Ethiopia; 2Department of Microbiology and Molecular Genetics, The Institute of Medical Research Israel-Canada The Kuvin Center for the Study of Infectious and Tropical Diseases, Faculty of Medicine, The Hebrew University, Hadassah Medical School, Jerusalem, Israel; 3Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia; 4Department of Microbiology, Immunology and Parasitology, Faculty of Medicine, Addis Ababa University, Addis Ababa, Ethiopia. [email protected]

Keywords: Bloodmeals, P. orientalis, host preference, Kala-azar Host choice and feeding success of sand flies are important factors for understanding the epidemiology and for developing efficient control strategies for visceral leishmaniasis. The aim of the study was to determine the host preference patterns of Phlebotomus orientalis from Tahtay Adiyabo district, Northern Ethiopia. Host attractiveness experiments were conducted by presenting wild sand flies with a choice of seven baits (human, cattle, sheep, goat, donkey, dog and chicken) and control (without bait) in locally designed tent traps. For blood meal analysis, sand flies were collected from three villages using CDC light and sticky traps. DNA was extracted from the blood fed sand flies and PCR amplified for vertebrate-specific cytochrome (cyt) b region, followed by reverse-line blot (RLB) analysis. Attraction and feeding success of P. orientalis indicated significant differences (P < 0.005) between baits. Abundance of P. orientalis on traps baited with cattle was higher than other animal baits and controls. Female sand flies were significantly more attracted to donkey and cattle than human, dog, goat, sheep or chicken-baited traps. P. orientalis females engorged more frequently on donkey and cows than they did on other hosts (goat, sheep, dog, and chicken). Out of 180 P. orientalis tested for the source of bloodmeals by cyt b PCR and RLB, the blood meals of 135 were successfully identified. P. orientalis mainly fed on bovine followed by human, goat, sheep and camel. Mixed blood feeding was also identified in ten female P. orientalis. The epidemiological significance of these domestic animals as blood sources for P. orientalis and possible reservoir hosts of L. donovani should be thoroughly investigated for better understanding the transmission dynamics of visceral leishmaniasis in northern Ethiopia.

Keywords: Visceral leishmaniasis, Phlebotomus orientalis, highland, Ethiopia The bionomics of phlebotomine sandflies (Diptera: Psychodidae) were studied for one year from May 2011 to April 2012 in three villages of Libo-Kemkem district, a highland area where visceral leishmaniasis has become a major public health problem. The aim of the present study was to elucidate species composition, resting habits, seasonal fluctuations of phlebotomine sandflies and incrimination of the vector. CDC light trap, sticky trap and knockdown space spray were used for collection of sandflies. Dissection and molecular approaches were used for Leishmania detection from female Phlebotomus that were collected by using CDC light traps. A total of 10, 776 sandflies comprising of two species of the genus Phlebotomus and five species of the genus Sergentomyia were collected. Phlebotomus orientalis was the predominant species accounting 86.6% of the total sandfly captured. The remaining species in descending order were Sergentomyia bedfordi group, S. squamipleuris, S. schwetzi, S. africana, S. clydei and P. rodhaini. Sandflies were present in most part of the year, except during the main rainy season (July-September). Phlebotomus orientalis exhibited two peaks of density, a smaller in January 2012 (6.5flies per trap night for CDC light trap; 0.98flies/m2/night for STs) and the larger one in March for STs (1.06flies/m2/night) and April for CDC light trap (18. 7flies per trap night). Abundance of the flies were positively correlated with average temperature (r=0.53 for CDC; r=0.563 for STs) and negatively correlated with rainfall (r= -0.272 for CDC; r= 0.171 for STs). The male/female ratio of P. orientalis in the total captures was 1.87:1. Behaviorally, P. orientalis exhibited both exophilic and exophagic. A total of 1060 P. orientalis and 1 P. rodhaini dissected were found to be negative by microscopic examination. A similar result was obtained when 247 P. orientalis females were processed by using Polymerase chain reaction for detection of the parasite. Although natural infection was not detected in the current study, P. orientalis is seems to be a vector of VL in this highland area of the country mainly due to its densely populations and other circumstantial evidences. Therefore, control of the disease in this particular area should involve designing of tactics that mainly target the vector of the disease by considering its seasonal abundance and behaviors.

N° 26 (1) 2015

Boletín de la Sociedad Entomológica Argentina

ID 38-O Ecology & Epidemiology

ID 39-O Ecology & Epidemiology

SPECIES COMPOSITION, ABUNDANCE AND SEASONAL DYNAMICS OF PHLEBOTOMUS SPECIES IN A VISCERAL LEISHMANIASIS ENDEMIC AREA OF NORTHWEST ETHIOPIA

PHLEBOTOMINE SAND FLIES CROSSING A N AT O L I A : D O B O R D E R S L I M I T T H E I R DISTRIBUTION?

Solomon Yared, Meshesha Balkew, Alon Warburg, Asrat Hailu and Teshome Gebre-Michael

O Kasap Erisoz1, A Belen2, C Alkan2, F Gunay1, V Dvorak3, K Ergunay4, S Aydın5, J Votypka3, A-L Banuls6, R Charrel2, A Özkul7, Y Özbel8, P Volf3, B Alten1

Addis Ababa University. [email protected]

Keywords: Visceral leishmaniasis, Phlebotomus spp, P.orientalis, Habitat preference Visceral leishmaniasis (VL) is significant public health problem in northwest Ethiopia particularly in Kafta Humera district. This study was conceived to investigate the species composition and population dynamics of sandflies (altitude 500-600masl) in five urban and semiurban area of Kafta Humera district namely Setit Humera, Mykadra, Rawiyan, Bereket and Adebay. Sandflies were collected for three nights monthly from May 2011 to April 2012 using CDC light-traps and sticky traps. Traps were placed within villages, at periphery of villages and farm fields. Sticky traps were also used for sampling indoor active sandflies. A total of 13097 sand flies comprising of six Phlebotomus species of four subgenera were indentified: Phlebotomus (Larroussius) orientalis, P.(Phlebotomus) papatasi, P.(Ph.) bergeroti, P.(Ph.) duboscqi, P. (Paraphlebotomus) alexanderi, and P. (Anaphlebotomus) rodhani. In addition, two Parvidens (P. lesleyae and P.heischi) species were recorded in the study area. Among these, P. orientalis was the most predominant species, accounting for (58.12%) followed by P. papatasi (29.62%), P. lesleyae (5.61%), P. bergeroti (3.80%), and P. duboscqi (2.07 %), P. alexandri (0.37%), P. heischi (0.24%) and P. rodhaini (0.18 %). On average, significantly higher densities of P. orientalis were caught in the periurban area of Adebay with compared to the urban area of Setit Humera. Overall, 684 Phlebotomus females were dissected for detection of Leishmania infection, but none was infected. Significant positive correlation was found between the monthly abundance of P. orinetalis and P. papatasi and the monthly averages temperature (P1.5, p