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Staphylococcus aureus , IL-10, Ekstrak Daun Pagoda. Correspondence ... semuanya diberikan pakan standar, kelompok kontrol positif (Amoxicilin dengan dosis.

Qanun Medika Vol. 3 No. 1

Januari 2019

Laporan Hasil Penelitian

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THE EFFECT OF PAGODA LEAF EXTRACT (CLERODENDRUM PANICULATUM L) ON THE IL-10 LEVEL IN MAMMAE OF FEMALE Laporan Hasil Penelitian RATS STRAIN (SPRAGUE DAWLEY) INDUCED WITH STAPHYLOCOCCUS AUREUS BACTERIA 1 2 3 4 Israini Israini Suriati Suriati1,, Mardiana Mardiana Ahmad Ahmad2,, Nurul Nurul Aini Aini Siagian Siagian3,, Prihantono Prihantono4 1) 1)

Midwifery Midwifery Academy Academy Muhammadiyah Muhammadiyah Palopo Palopo Midwifery Academy Muhammadiyah Palopo Midwifery Academy Muhammadiyah Palopo 3) 3) Fakultas Fakultas Kebidanan Kebidanan Deli Deli Husada Husada Delitua Delitua Medan Medan 4) 4) Clinical Clinical Pathology Pathology Department Department Medical Medical Faculty Faculty Hasanuddin Hasanuddin University University Makassar Makassar 2) 2)

Submitted Agustus : Desember Accepted : Januari Published : Januari Submitted: 2018 Desember 2018 Januari 2019 Submitted: Agustus 20182018Accepted: Accepted: Desember 20182019Published: Published: Januari 2019 2019

Qanun Medika vol. no. |bulan tahun

ABSTRAK ABSTRAK ABSTRACT Mastitis Mastitis adalah adalah masalah masalah penyakit penyakit infeksi infeksi yang yang ditemukan ditemukan pada pada ibu ibu menyusui menyusui yang yang biasanya biasanya Mastitis is an infectious disease problems of found in breast feeding usually caused by disebabkan jaringan disebabkan oleh oleh Staphylococcus Staphylococcus aureus aureus.. Ketika Ketika bakteri bakteri Staphylococcus Staphylococcus aureus aureus masuk masuk ke ke jaringan Staphylococcus aureus . When Staphylococcus aureus bacteria enters the body’s tissues, IL-10 tubuh, tubuh, kekebalan kekebalan IL-10 IL-10 akan akan aktif aktif membantu membantu mencegah mencegah selsel- sel sel rusak. rusak. Sistem Sistem imun imun akan akan immune will prevent cell broken.dibutuhkan The immune system will get worse if there is no memburuk jika tidak ada Sehingga terapi komplementer seperti tanaman memburuk jikaactive tidak helped ada penanganan. penanganan. Sehingga dibutuhkan terapi komplementer seperti tanaman treatment. So that it takes complementary therapies like pagoda leaves, The content of the pagoda daun daun pagoda, pagoda, kandungan kandungan dari dari tanaman tanaman daun daun pagoda pagoda terdiri terdiri dari dari tannins, tannins, alkaloids alkaloids and and flavonoids, flavonoids, contains tannins, alkaloids and flavonoids, where is this compound can to improve di mana senyawa ini dapat meningkatkan sistem kekebalan tubuh. Jenis penelitian yang di mana senyawa ini dapat meningkatkan sistem kekebalan tubuh. Jenis penelitianimmune yang . This study aims to determine the effect of pagoda leaf extract on IL10 levels in rats system digunakan digunakan dalam dalam penelitian penelitian ini ini adalah adalah true true experimental experimental dengan dengan rancangan rancangan pre pre dan dan posttest posttest induced by Staphylococcus aureus bacteria. The type of research used in this study is true control control design. design. Yang Yang menggunakan menggunakan tikus tikus strain strain Sprague Sprague Dawley Dawley sebanyak sebanyak 15 15 ekor ekor yang yang dibagi dibagi experimental with pre andkelompok posttest control use pro of 15 Sprague Dawley rats dalam 33 kelompok yaitu kontrol negatif (aqua injections) sebanyak 55 ekor dalam kelompok yaitu kelompok kontroldesign. negatifThe (aqua pro injections) sebanyakstrain ekor divided into 3 groups: 5 negative control groups (aqua pro injections 1ml/gramBB) five tails were semuanya semuanya diberikan diberikan pakan pakan standar, standar, kelompok kelompok kontrol kontrol positif positif (Amoxicilin (Amoxicilin dengan dengan dosis dosis all given standard feed, positive control group (Amoxicilin with 9.6ml / 250gramBB ) five tails 9,6ml/250gramBB) sebanyak 5 ekor semuanya diberikan pakan standar, dan kelompok 9,6ml/250gramBB) sebanyak 5 ekor semuanya diberikan pakan standar, dan kelompok were all given standard feed, and treatment group (Amoxicilin 9.6ml / 250gramBB plus pagoda perlakuan perlakuan (Amoxicilin (Amoxicilin 9,6ml/250gramBB 9,6ml/250gramBB ditambah ditambah dengan dengan ekstrak ekstrak daun daun pagoda pagoda leaf extract 50ml/250gramBB) five tails were all given standard feed. All groups were induced (50ml/250gramBB) pakan standar. Semua kelompok diinduksisikan (50ml/250gramBB) 55 ekor ekor semuanya semuanya diberikan diberikan pakan standar. Semua kelompok diinduksisikan 8 bacteria Staphylococcus aureus mlx10Pemeriksaan ml/CFU). IL-10 levels were examined using theRRR& & bakteri Staphylococcus aureus 0,2 ml/CFU. kadar IL-10 menggunakan metode bakteri Staphylococcus aureus 0,2(0,2 ml/CFU. Pemeriksaan kadar IL-10 menggunakan metode & D method of the Enzyme Linked Immonosorbent Assay (ELISA) Rat system. Data analysis used D D system system Enzyme Enzyme Linked Linked Immonosorbent Immonosorbent Assay Assay (ELISA) (ELISA) Rat. Rat. Analisis Analisis data data menggunakan menggunakan uji uji ANOVA+++ post post hoc, hoc test, withnilai a significant 0, 05. This study showed no difference in ANOVA dengan signifikan ρρ ≤≤ ρ0, Penelitian ini tidak ANOVA post hoc, dengan nilai signifikanvalue 0,≤05. 05. Penelitian ini menunjukkan menunjukkan tidak ada ada levels of IL10 cytokines, beforesebelum induceddiinduksikan Staphylococcus aureus bacteria in allaureus groupspada withsemua values perbedaan kadar sitokin bakteri Staphylococcus perbedaan kadar sitokin IL-10, IL-10, sebelum diinduksikan bakteri Staphylococcus aureus pada semua (ρ> 0.05). Whereas after the treatment was given, the value of ρ 0,05).. Sedangkan setelah diinduksikan bakteri Staphylococcus kelompok dengan nilai (ρ > 0,05).. Sedangkan setelah diinduksikan bakteri Staphylococcus that there difference in IL10mengalami levels after treatmentkadar between groups.nilai In the aureus ±± 24 jam kelompok penurunan IL10 (ρ 0,05). aureus 24was jama semua semua kelompok mengalami penurunan kadar IL10alldengan dengan nilai (ρ >>negative 0,05). control group, IL10 experienced a decrease (mean ± 10.4 pg / ml). While the increase in IL-10 Setelah Setelah diberikan diberikan treatment treatment diperoleh diperoleh nilai nilai ρρ 0,05).. Sedangkan setelah diinduksikan bakteri Staphylococcus lactation, so it is also called lactational aureus ± 24 jam semua kelompok mengalami penurunan kadar IL10 dengan nilai (ρ > 0,05). mastitis or commonly referred to as Setelah diberikan treatment diperoleh nilai ρ < 0,05) antara semua kelompok hal ini menunjukkan puerperal mastitis. This situation can be ada perbedaan kadar IL-10 setelah diberikan treatment antara semua kelompok. Pada kelompok fatal if± it10,4 is not handled properly. Mastitis kontrol negatif kadari IL-10 mengalami penurunan (rerata pg/ml). Sedangkan peningkatan generally occurs around 1 to 6 weeks kadar IL-10 lebih besar pada kelompok perlakuan (rerata ± 58.4 pg/ml), dibandingkanpost pada partum. complications thatekstrak will occur kelompok positif (rerata ± 41.8 pg/ml ). Kesimpulan dalamThe penelitian ini adalah daun pagoda (Clerodendrum Paniculatum L) memiliki pengaruh meningkatkan kadar The IL-10 in mastitisdalam are abscess in the breast. sebagai anti-inflamasi. Ekstrak daun pagoda dapat dijadikan terapi komplementer. incidence of mastitis according to the world health organization that Qanun Medika no. estimates |bulan tahun Kata kunci : Staphylococcus aureus, IL-10, Ekstrak Daunvol. Pagoda more than 4.1 million people are Korespondensi : [email protected] diagnosed and an estimated (IL-10) iswith an mastitis, anti-inflammatory cytokine INTRODUCTION 3-20% of breastfeeding mothers can containing anti-inflammatory properties Breast inflammation or commonly experience mastitis, the 1 until 10 women that can suppress pro-inflammatory referred to as mastitis is a disease that can (10%) who experienced first in reaction Interleukin mastitis 10 has inatherole be accompanied by infection or not month after thedelivery. The response signs and to limiting immune accompanied by infection, while the type symptoms of mastitis such as: Fever in the to pathogens, thus preventing damage of bacteria that most often causes mastitis body, especially in the breast and tissues and maintaining connective tissue is the Staphylococcus aureus bacteria. experience inflammation of levels the breast homeostasis. Interleukin-10 as antiThis disease usually accompanies accompanied by pain. The causes of inflammatory cytokines will decrease if lactation, so it is also called lactational mastitis include: starting from being there is inflammation in the body (Couper mastitis or commonly referred to as infected by etbacteria, N Kevin, al 2008; scuffed Rayitno nipples, A, et al, puerperal mastitis. This situation can be improper breastfeeding techniques, 2014; Garcia YF, et al, 2011; Iyer fatal if it is not handled properly. Mastitis infrequent breastfeeding frequency or Subramanian S, et al, 2012; Kumar Ashok, generally occurs around 1 to 6 weeks post short times, overly et al,breastfeeding 2000; AmirH. Lisa,and et al, 2014)fast partum. The complications that will occur weaning and attachment of babies to poor Inflammation when left without treatment in mastitis are abscess in the breast. The breasts, and babies who only sucking will result in autoimmune or incidence of mastitis according to the nipples (not including the areola) causing autoinflamation disorders, world health organization estimates that the nipple to squeeze between the gums or neurodegenerative diseases, or cancer. As more than 4.1 million people are lips so that the milk flow is imperfect for treatment so far which is still often diagnosed with mastitis, and an estimated (WHO, 2000: Tri Anasari, Sumarni. 2014: used for inflammation is conventional 64 3-20% of breastfeeding mothers can Amir H Lisa, et al, 2014: IDAI 2013; treatment. Even though there are now experience mastitis, 1 until 10 women Anasary Tri Anasari, Sumarni. 2014 ; al, many herbal plants that can be used as (10%) who experienced mastitis in the first 2012; Dixon Michael J, et. al 2011: Khanal complementary therapies, one of them is month after delivery. The signs and Vishnu, et al. 2015: Viduedo Silva, et al

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(IL-10) i containin that can reaction limiting pathogen tissues an homeosta inflamma there is in N Kevin 2014; G Subraman et al, 200 Inflamma will re autoinflam neurodeg for treatm used for treatment many her complem the pago paniculat Shrivasta From the that pa compoun alkaloids, glycoside (Joseph J research pagoda le medicine typhoid, (M. Prav 2013) From the suggest t the pago active co

mmonly hat can or not he type mastitis acteria. mpanies tational to as can be Mastitis ks post ll occur st. The to the es that le are timated rs can women the first ns and er in the st and breast uses of being nipples, niques, ncy or rly fast to poor sucking causing gums or mperfect i. 2014: I 2013; 14 ; al, Khanal

that pagoda plants contain active infrequent breastfeeding frequency mastitis include: starting from being or (Joseph Jeenu, 2013). From thetannins, results of compounds such as: flavonoids, short breastfeeding times, and overly fast infected by bacteria, scuffed nipples, research conducted in India showed alkaloids, phenolic acids, sterols,that weaning and attachment of babies to poor improper breastfeeding techniques, pagoda leaves areacids, also used as traditional glycosides, phenolic and glycosides breasts, and babies who only sucking infrequent breastfeeding frequency or medicine Korea,From for the HIV, Syphilis, (Joseph Jeenu,in2013). results of nipples (not including short breastfeeding times,the andareola) overlycausing fast typhoid, Cancer, Jaundice, hypertension research conducted showed 2019 that the nipple squeeze between or weaning and to attachment of babiesthe to gums poor Qanun Medika Vol. 3 No.in1 India Januari (M. Praveen, et al., 2012; Thakur, et al., pagoda leaves are also used as traditional lips soand thatbabies the milk imperfect breasts, whoflow onlyis sucking 2013) in Korea, for HIV, Syphilis, medicine (WHO, Tri Anasari, Sumarni. 2014: nipples (not2000: including the areola) causing From the various facts facts above which typhoid, Cancer, Jaundice, hypertension et between al, 2014:theIDAI theAmir nippleHto Lisa, squeeze gums2013; or thatetpagoda plants on theet part (M.suggest Praveen, al., 2012; Thakur, al., of Sumarni. 2014 ; al, lipsAnasary so thatTri theAnasari, milk flow is imperfect the pagoda roots and flowers contain 2013) 2012;2000: DixonTri Michael J, et. al 2011:2014: Khanal (WHO, Anasari, Sumarni. active compounds as antiFrom the various factsthat factsfunction above which Vishnu, et al.et2015: Viduedo et al Amir H Lisa, al, 2014: IDAISilva, 2013; inflammatory which areof so suggest that pagodaagents plants on the part 2015: Tri Baratawidjaja K, et2014 al, ; 2014; Anasary Anasari, Sumarni. al, effective in curing various diseases such as the pagoda roots and flowers contain Manzanillo Paolo, J,etet. al,al2015). 2012; Dixon Michael 2011: Khanal like compounds cancer, infections, boils. as In anticertain active that function Interleukin 10 (IL-10) including type Vishnu, et al. 2015: Viduedo Silva, et al II Qanun Medika vol. no. |bulan tahun areas the pagoda is strongly to be inflammatory agents whichbelieved are so cytokines. This typeK,of et cytokine has an 2015: Baratawidjaja al, 2014; a complementary therapy in certain effective in curing various diseases such as organizational binds to receptors Manzanillo Paolo,form et al,and 2015). that typhoid researchers were likediseases. cancer, So infections, boils. In certain (IL-10) is an anti-inflammatory cytokine that are similar in structure. Interleukin-10 Interleukin 10 (IL-10) including type II areas the pagoda is strongly believed to be containing cytokines. Thisanti-inflammatory type of cytokine properties has an halaman a complementary therapy in certain that can suppress thebinds pro-inflammatory organizational form and to receptors interested research were for diseases. Sointhatconducting typhoid researchers reaction Interleukin 10 Interleukin-10 has a role in that are similar in structure. pagoda leaves for mastitis where the title limiting the immune response to of the penetration was the effect ofhalaman giving pathogens, thus preventing damage to extracts of pagoda leaves (clerodendrum tissues and maintaining connective tissue paniculatum L) to the levels of il-10 in homeostasis. Interleukin-10 levels as antifemale mice induced by the bacterium inflammatory cytokines will decrease if staphylococcus aureus " besides that there is inflammation in the body (Couper pagoda leaves are available especially in N Kevin, et al 2008; Rayitno A, et al, South Sulawesi, Elisa examination can be 2014; Garcia YF, et al, 2011; Iyer done at the Hasanuddin University Subramanian S, et al, 2012; Kumar Ashok, Hospital in Makassar. As well as research et al, 2000; AmirH. Lisa, et al, 2014) on the benefits of pagoda leaf extract as an Inflammation when left without treatment anti- inflammatory on mammae sprague will result in autoimmune or dawley mice induced by autoinflamation disorders, Staphylococcus aureus bacteria has never neurodegenerative diseases, or cancer. As been done for treatment so far which is still often used for inflammation is conventional treatment. Even though there are now METHODS many herbal plants that can be used as The type of research used in this study is complementary therapies, one of them is pure experimental or true experimental, the pagoda leaf plant (Clerodendrum which is an experiment conducted in the paniculatum L: Hafiz Ihsanul, et al 2016). laboratory, with pre and post test control Shrivastava, et al, 2007). design. The group was divided into 3 From the phytochemical test results found (three) groups: negative control group, that pagoda plants contain active positive control group, treatment group. compounds such as: flavonoids, tannins, This research was carried out in the alkaloids, phenolic acids, sterols, UNHAS Hospital laboratory for glycosides, phenolic acids, and glycosides examination for Enzyme Linked (Joseph Jeenu, 2013). From the results of Immunosorbent Assay (ELISA) and research conducted in India showed that culture of Staphylococcus aureus pagoda leaves are also used as traditional bacteria, Biopharmaca laboratory for medicine in Korea, for HIV, Syphilis, drying plants, Pharmacy Laboratory of typhoid, Cancer, Jaundice, hypertension State Islamic University (UIN) Makassar (M. Praveen, et al., 2012; Thakur, et al., to extract and phytochemical testing, 2013) 65 animal laboratory UNHAS for the process From the various facts facts above which of rats adaptation to the end of the suggest that pagoda plants on the part of treatment given. the pagoda roots and flowers contain The sample in this study were Sprague

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50ml/250g standard w The instrum materials, na Luwu, Sou people's hou the UNHA previously th kg of raw le then sifted sample. Aft extracted by obtained ± 5 experimenta rats strain S 250 grams. (for negative (For positive Staphylococ standard . T making a sus 10 ml mix bacteria col turbidity lev Accuracy o measured by dosage is inj 0,2 mlx108 m is carried ou the breast p duct in rats i and treatmen is then obse infection re (There are si The research carried out Immunosorb IL-10 levels.

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Sprague of about rats strain

examination for Enzyme Linked Accuracy of turbidity level Mc Farland positive control group, treatment group. is carried out by intramuscular technique in Immunosorbent Assay (ELISA) and measured by the Densi check tool. So that the This research was carried out in the the breast part precisely in the lactiferous culture of Staphylococcus aureus dosage is injected into as many rats as possible duct in rats in all8 groups (Negative, positive UNHAS Hospital laboratory for bacteria, Biopharmaca laboratory for 0,2 mlx10 ml/CFU. Bacterial induction and treatment) after the induction of bacteria examination for Enzyme Linked drying plants, Pharmacy Laboratory of carried out intramuscular technique in is thenis observed ±24byhours later to see an Immunosorbent Assay and Medika Qanun Vol. 3 No. 1precisely Januari 2019 State Islamic University (UIN)(ELISA) Makassar the breast part in the lactiferous infection reaction in the rat breast area of phytochemical Staphylococcus aureus to culture extract and testing, in ratsofininfection all groups (Negative, (Thereduct are signs in the breast) positive bacteria, Biopharmaca animal laboratory UNHAS for laboratory the process for and treatment) after the inductionwas of bacteria The research data collection instrument plants, Pharmacy Laboratory of drying rats adaptation to the end of the of is then observed ±24 hours later to see an carried out by method Enzyme Linked State Islamic treatment given. University (UIN) Makassar infection Assay reaction in theto rat breast area Immunosorbent (ELISA) measure to sample extractin and phytochemical testing, The this study were Sprague are signs of infection in atthe IL-10(There levels. Histopathology test done thebreast) animalrats laboratory UNHAS forofthe process Dawley with a body weight about The research data collection instrument was hospital University of Hasanuddin of 250 ratsgrams. adaptation endstrain of the 200With usetoof the 15 rats carried out by method Enzyme Makassar. The tissue taken for the sample is Linked treatment given. Sprague Dawley divided into 3 groups: 5 Assay (ELISA) to measure breastImmunosorbent tissue in all groups. The sample in this study(aqua were Sprague negative control groups pro IL-10 levels. Histopathology test done This research was approved and issued by at the Dawley 1ml/gramBB) rats with a body of about injections wereweight all given hospital University of Hasanuddin the Ethical Commitment of the University of 200- 250 grams. Withno. use of 15 group rats strain standard feed, positive control Qanun Medika vol. |bulan tahun Makassar. The tissue taken for the sample is Hasanuddin Makassar on February 5, 2018 Sprague Dawley divided groups: (Amoxicilin with 9.6ml ) as 50ml/250gramBB) 5/ 250gramBB rats into were3 all given5 tissue/ inH4.8.4.5.31 all groups. / PP36with breast No. 116 negative control groups (aqua many as 5 rats were fed standard, and pro standard were all all given standard feed. KOMETIK / 2018. was approved and issued by This research group feeding (Amoxicilin 9.6ml / injections 1ml/gramBB) were all include given The instruments used in this study Data the is Ethical processed and analyzed Commitment of the using University of 250gramBB plus pagoda pagoda leaf extract standard namely feed, positive control group materials, leaves from Belopa, computer assistance, to see on the February effect of 5, 2018 Qanun Me Hasanuddin Makassar (Amoxicilin 9.6ml / 250gramBB as Luwu, Southwith Sulawesi, precisely in ) the givingwithpagoda leaves/ H4.8.4.5.31 (Clerodendrum/ PP36No. 116 many ashouse. 5 rats And werecarried all fedout standard, and people's extracted in paniculatum L), to /levels KOMETIK 2018.of IL10 cytokines. halaman groupUNHAS feeding Biopharmaca (Amoxicilin laboratory, 9.6ml / the IL-10 Data levelsisareprocessed seen usingand the analyzed method using 250gramBB plus pagoda leafpicked extract previously the pagoda leaves were ±5 Enzyme Linkedassistance, Immunosorbent computer to see Assay the effect of kg of raw leaves, after that it is cleaned first, (ELISA). Three levels of IL-10 were then sifted to obtain a smooth simplicia examined (3) time, blood collection firsthalaman sample. After that the sample is ready to be when rats have not been treated to assess extracted by maceration method, so that it is whether IL-10 levels are normal or not, then obtained ± 500 gram pagoda thick extract. The taking blood the second time after the mouse experimental animals used in this study were was induced by Staphylococcus aureus rats strain Sprague Dawley with weight 200bacteria, and taking blood last time when 250 grams. And needed Aqua pro injection mice were given good in the negative, (for negative group), and amoxicillin 500 mg, positive and treatment groups. (For positive groups), and the bacteria used are The bivariate test uses a test one way anova to Staphylococcus aureus bacteria strain see differences in IL-10 levels in the negative standard . To make a sample of bacteria by control group before, during and after making a suspension in solution NaCl as much treatment, positive control before, during and 10 ml mixed with Staphylococcus aureus after being given treatment, and treatment bacteria colonies that golden yellow with group before, during and after being given turbidity level Mc Farland 2 x 108 CFU. treatment and done. But if the data is not Accuracy of turbidity level Mc Farland normally distributed then a test is carried out measured by the Densi check tool. So that the Kruskal-Wallis. other than that also done test dosage is injected into as many rats as possible repeted anova if the data is normally 0,2 mlx108 ml/CFU. Bacterial induction distributed to see differences in IL-10 levels in is carried out by intramuscular technique in each group, namely the negative, positive, and the breast part precisely in the lactiferous treatment groups before, during and after duct in rats in all groups (Negative, positive being given treatment. and treatment) after the induction of bacteria is then observed ±24 hours later to see an infection reaction in the rat breast area (There are signs of infection in the breast) The research data collection instrument was carried out by method Enzyme Linked Immunosorbent Assay (ELISA) to measure 66 IL-10 levels. Histopathology test done at the hospital University of Hasanuddin Makassar. The tissue taken for the sample is

Qanun Medika vol.vol. no.no. |bulan tahun Qanun Medika |bulan tahun

Qanun Medika Vol. 3 No. 1

Januari 2019

RESULTS RESULTS Table 1. Phytochemical testtest results of the compound of Leaf Pagoda (Clorodendum Table 1. Phytochemical results of the compound of Leaf Pagoda (Clorodendum Paniculatum L). Paniculatum L). Sample Nama Sample Nama DaunPagoda DaunPagoda (Clorodendum (Clorodendum Paniculatum L). L). Paniculatum

Identification of Compound Groups Identification of Compound Groups Steroid/ Steroid/ Flavonoid Tanin Flavonoidtripernoid Alkaloid Alkaloid Tanin tripernoid + +

-- --

+ +

+ +

Qanun Medika vol.compounds no. |bulan tahun Describsion : + (positive): there are are indications of bioactive Describsion : + (positive): there indications of bioactive compounds Table 2. The mean body weight of mice in each group Weight

Mean ± SD

Min-Maks

Nilai ρ

226 ± 12 213 – 239 Negative control group 220 ± 8 208 – 233 Poaitive control group 0, 56 220 ± 10 208 – 231 Treatment Group *One way ANOVA. Significant value ρ 5 0.05. There were no differences in body weight in all groups, both in the negative, positive and treatment control groups (ρ = 0.56) Table 3:Average differences in IL-10 levels in each female rat group Sprague Dawley strain before, after induction of Staphylococcus aureus bacteria and after treatment. IL-10 levels (pg / ml) Before Induction Mean ± SD

After Induction Mean ± SD

After Treatment Mean ± SD

Value ρ

111,2 ± 10,5

34,5 ± 8,2

24,1 ± 5,6

0.00a

115,1 ± 16,9

33,7 ± 8,2

75,4 ± 7,6

0.001a

Treatment (Antibiotic Amoxicilin 9,6ml/250grBB + pagoda leaf extract50 ml/grBB (n =6)

111,9 + 19,6

31,8 + 16,1

90,3 + 11,4

0,003

Nilai ρ

0, 58b

0.68b

0.000

Group Negative Control (n =6) Positif Control (Antibiotic Amoxicilin 9,6 ml/250grBB ) (n =6)

The results of the statistical test of one way ANOVA before induced Staphylococcus aureus bacteria in each group obtained the value ρ = 0.58 greater than the value of α = 0.05. This means that there is no significant difference in the levels of IL-10 cytokines before induced Staphylococcus aureus bacteria between the negative, positive and treatment control groups. Whereas IL-10 levels after induced Staphylococcus aureus bacteria ± 24 hours in each group obtained values ρ = 0.68 greater than the value of α = 0.05.

Picture 1: Trend of IL-10 levels in each group of female rats with Sprague Dawly strain before, after induced Staphylococcus.Aureus and after treatment..

67

halaman halaman

leaf extract50 ml/grBB (n =6) Nilai ρ

0, 58b

0.68b

0.000

The results of the statistical test of one way ANOVA before induced Staphylococcus aureus bacteria in each group obtained the value ρ = 0.58 greater than the value of α = 0.05. This means that there is no significant difference in the levels of IL-10 cytokines before induced Staphylococcus aureus bacteria between the negative, positive and treatment control groups. Whereas IL-10 levels Medika after inducedVol. Staphylococcus bacteria ± 242019 hours in Qanun 3 No. aureus 1 Januari each group obtained values ρ = 0.68 greater than the value of α = 0.05.

Picture 1: Trend of IL-10 levels in each group of female rats with Sprague Dawly strain before, after induced Staphylococcus.Aureus and after treatment..

Qanun Medika vol. no. |bulan tahun Description: IL-10 levels in each group before induced Staphylococcus aureus bacteria (homogeneous), and after induced Staphylococcus aureus bacteria all groups experienced a decrease in IL-10 levels. Whereas after giving the treatment the positive control group and the treatment increased, the group while the negative control group decreased.

halaman Table 4: Analysis of differences in IL-10 levels before, after induced Staphylococcus aureus bacteria and after treatment in each group

Measurement

Before Treatment Negative Control Positive Control Negative Control Treatment Positive Control Treatment

Mean ± SD

111,2 ± 10,5 115,1 ± 16,9 111,2 ± 10,5 120,4 ± 16,7 115,1 ± 16,9 120,4 ± 16,7

After Inductions bakteria s. aureus Negative Control 34,5 ± 8,2

Positive Control Negative Control

33,7 ± 8,2 34,5 ± 8,2

Treatment Positive Control

30,8 ± 6,6 33,7 ± 8,2

Treatment After Treatment Negative Control

30,8 ± 6,6

Positive Control Negative Control

75,4 ± 7,6 24,1 ± 5,6

Treatment Positive Control

90,3 + 11,4 75,4 ± 7,6

Treatment

90,3 + 11,4

24,1 ± 5,6

Difference Mean

Nilai ρ*

-3.8

1,00

-9.1

0,92

-5.3

1,00

0,8

1,00

3,7

1,00

2,9

1,00

-51.3

0.00

-66.2

0.00

-14.9

0.56

Nilai Ρ

0,58

0, 68

0,00

Post hoc analysis before and after induced Staphylococcus 68aureus bacteria obtained a value of ρ> 0.05 between each group. It showed no difference in levels of IL-10 cytokines, both before and after induction of ± 24 hours of Staphylococcus aureus bacteria in all groups. Whereas after being given treatment, the value of p