T JJJ
r ;
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n]ls.
Effect of Fermented Product trom Bacillus subtilis on Feed Conversion Efficiency, Lipid Accumulation and Ammonia Production in Broiler Chicks
a
2.
nization
;
I
U. Santosor, K. Tanaka*. S. Ohtanir and trI. Snkaidal [-aboatoriur]r
cit."- in
9
Roie
tev
iL'rv.
ABSTBACT
NII I-N vali\.es,
of A:titnal
:
l'his sltrdl inreslilt!1"'d Ihe !'lJicts ol- llnncnr(l pr(xl,rcr lronr
of FPBS as lhc conlrol and
bul ilrlo.
o[ Biorcsourc.'s and Bioproducrion. Cradrnrc School Hokkaido Univcrsirv. Sapporo 060 0E09, Japan
NLrtrition, Division
2001. VoL 11, N'o.
3
:
tlrt
othc'r' thrce
1l rcr11as
of Asricultruc
srrbri/ir fFPBS) ()n 1cc,:l convcrsion
lrouPs NerL' 1e(l conrnrL'rciitl rlicis r'nraining FPBS. eiiIcr 0.-5, L0 or
I07.
tin
333-337)
INTRODUCTION
Reccntly, thcrc is grcutcr A$rArcness an,onl]!:i consumcrs conccnring dictarr fat anri its advcrsc cffccis on hunan hcalth. In ordci (o consLnrc nrcat with lorvcr fat contcnt, consulllcn tcnd lo consuntc nrcat of broilcrs slaughtcrccl lll loLrngcr agcs (28 dal,s of age). Horvcvcr-, ccononric rctunrs arc lorvcr as comparcd to convcnlienill slaLrghtcr 1t.12 dlvs oi agtr (Sanloso ct al , i998). Thcrcforc. an rr.nrpr shoLrlci ir.' nrade lo inrprovc ptocluction bl itnprovirtr lced convcrsion cliicicncy ancl bclclr rvcight r'ain at y,ourrgcr agcs ibcforc 42 da),s). Althorrgh rt an carly agc broilcr chicks raiscd ir a u,inclorvlcss l:ousc prorlucccl lcss aruronia gas lltan at latc: agc (Nishilurtra, 199-1), lhc conccD(mliol is still highcr thtn thc silic lcvcl. Wilaya (2000) statcd that anrnolrir al 20 ppnr rcduccd body wcight arld fccd convcrsion cfficicncy. Thcrcforc, a lower ammonia gas releasc is ngcdcd. Previous cxperimcnrs sho*,ed that thc inciusion of microorganisms in thc diets improved fecd convenion efficjency (Jirapochakul et al., 1990; Santoso et al., 1995) and reducod fat accumulation of broilcr chicks
*
Address reprint reqresl 1o K. Tanaka. Tel & Fax: +81l-70b-2470. L.-mail:
[email protected] ac.jp. ' Depaflmenr oI Animal Science. Faculr) of Agricullure. I
Bengkulu UniveNity, Indonesia.
- Department of Animal Science and Technology, oI Agricukure. Cifu Univenity, Jepan.
Faculty
' Japan Biotics Company. Received May 10, 2000; Accepred November 17, 2OO0
r1 .11 (la!s oI agc (Slnritso ct al., 1995; Tanlka c( lrl., l99t ) Sanlos.r cl al. (1999) sl,orvcci that fccding driccl
Batillus subtilis cullLue al 0-5% irclusioo lcvcl thc rclcasc of antmonia gas. Thc prcscnt stutlv, thcrcfbrc, invcstigalccl Ihc cflcct of comrncrcial li'rnrcrttcd prorluct fronr llatillus subtilis (FPBS) on
rcclrrccci
fccd convcrsion cflicicrrcv, l'at accurlulaiion anrnoria gau rclcasc al 18 Cal's of agc
and
MATERIALS AND METHODS Fcmalc broilcr chicks (strair Clrunky) Lrscrl in rhis a cornnrcrcial hatclrcry. At 7 da)s of agc, thcy \!,crc \!,cighcd indivi(lrrall\', and sixl] b:oilcr chicks rvcrc sclcctccl, u,int-blndcd, ranclon-,ly slLr(ly wcrc obtaincrl fronr
disrribulcd to lbrlr lrcatllcnl groups and raiscd in incliviclual cagcs. From I la 14 da_vs of agc, supplcrncntal bcat rvas providcd, and Ihcrcaflcr thc room lcmpcraturc was kcpl at 221 2t \r,ith (he lighl on from 08:00 to 20;00. Thc chicks were raiscd to 28 days of agc in individual cages. Onc group was fcd a commercial diet without supplementation of FPBS as the control, and thc othcr thrcc groups rvere fed contmercial dicts supplcmcntcd with eithcr 0.5, 1.0 or 1.0% oi' FPBS for' 2i ciays from 7 to 28 days of age. Commcrcial FPBS was produced by fermenting the media using Bacll/as sabtills, dricd, ground and pelleted. Water and. feed were fed ad. libitwn. Body weight was individually measured on a wcekly basis. The composition of diets is presented in table L
SANTOSO ET AL,
334
At the end of the experimertal period (28 day of of each group were killed, and livers and weighed. Five gram of liver was were removed placcd in icc-cold salinc to mcasure thc activitics of age), ten chicks
lipogenic enzymes, and the remainder of the liver was frozen arrd srored at -30C for thc analysis of various
lipid fractioi's. Bioc
