Veterinary World, EISSN: 2231-0916 Available at www.veterinaryworld.org/Vol.9/February-2016/12.pdf
RESEARCH ARTICLE Open Access
Effect of incubation on freezability of cholesterol-loaded cyclodextrin treated buffalo (Bubalus bubalis) spermatozoa S. A. Lone1, J. K. Prasad2, S. K. Ghosh2, G. K. Das2, B. Balamurugan2, R. Katiyar2 and M. R. Verma3 1. Division of Animal Reproduction, Gynecology & Obstetrics, National Dairy Research Institute, Karnal - 132001, Haryana, India; 2. Germplasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly - 243122, Uttar Pradesh, India; 3. Division of Livestock Economics and Statistics, Indian Veterinary Research Institute, Izatnagar, Bareilly - 243122, Uttar Pradesh, India. Corresponding author: S. A. Lone, e-mail:
[email protected], JKP:
[email protected], SKG:
[email protected], GKD:
[email protected], BB:
[email protected], RK:
[email protected], MRV:
[email protected] Received: 02-09-2015, Revised: 21-12-2015, Accepted: 29-12-2015, Published online: 18-02-2016 doi: 10.14202/vetworld.2015.182-185 How to cite this article: Lone SA, Prasad JK, Ghosh SK, Das GK, Balamurugan B, Katiyar R, Verma MR (2016) Effect of incubation on freezability of cholesterol-loaded cyclodextrin treated buffalo (Bubalus bubalis) spermatozoa, Veterinary World 9(2): 182-185.
Abstract Aim: The aim of this study was to investigate the effect of incubation on freezability of cholesterol loaded cyclodextrin (CLC) treated buffalo spermatozoa. Materials and Methods: Semen samples with mass motility of 3+ and greater, collected from Murrah buffalo bulls were utilized. Immediately after collection, four equal groups of semen sample were made. Group I was kept as control and diluted with Tris upto concentration of 60×106 sperm/ml, where as Groups II, III, and IV were treated with CLC at 3 mg/120× 106 spermatozoa, incubated at 37°C for action of CLC for 10, 15 and 20 min, respectively, and diluted with tris upto concentration of 60×106 sperm/ml. All groups were subjected to equilibration and freezing. The evaluation of semen samples from all groups was carried out at fresh, pre-freeze and post-thaw stage for progressive motility, viability and hypoosmotic swelling response (HOS response). Results: At the pre-freeze stage, significantly (p
