Effect of PENS on the ileal digestibility of dietary

Effect of PENS on the ileal digestibility of dietary protein in pigs – preliminary results Janczyk P., Pieper R., Urubschurov V., Souffrant W.B.

Research Unit Nutritional Physiology, Research Institute for the Biology of Farm Animals, Dummerstorf, Germany

Dr. P. Janczyk Bristol 13-14.12.07

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Animals Purebred German Landrace piglets One father for all piglets (Nadler) Trials

Pigs weaned and kept under standardized experimental conditions (experimental swine facility - EAS)

Pigs weaned and kept inpractice under commercial conditions (Pankelow – Wiechmann)

Ileo-rectal anastomosis and tracer trial (15N) (WP3) Dr. P. Janczyk Bristol 13-14.12.07

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Objectives

Effect of PENS on the ileal protein digestibility

Effect of PENS on ileal nitrogen composition – dietary, endogenous, microbial


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Material and methods

Technique Jugular vein catheterisation Ileo-rectal anastomosis


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cannula


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Experimental design 24 Landrace male pigs at 28 d

day

1

2

3

4

5

6

7

8

9

10

11 12 13 14 15 16 17 18

19 8.00

15N-yeast

orally at 8.00 and 18.00 surgery

adaptation

“0” blood recovery sample “0” digesta sample


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no yeast

e x i blood sampling at 8.00 and 18.00 t u left-over s and digesta collection

Diets & groups Group (n=4)

Diet

RF

Reference diet

Starter A

RF + 1.0% Chlorella vulgaris

Starter B

RF + 0.1% Na-alginate

Starter C

RF + 1.5% inulin

Starter D

RF + 0.04% oil mix 1

Starter E

RF + 0.04% oil mix 2


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Calculations %15N-excessplasma = %15Nplasma”i,j” - %15Nplasma”0” “i,j” –12 or 24 hours before the end of the collection period “0” – sample collected before start of 15N feeding

%15N-excessdigesta = %15Ndigesta”i,j” - %15Ndigesta”0” “i,j” – digesta collected over 48 - 24 and 24 – 0 hours before the end of the experiment “0” – digesta collected over 24 hours before start of 15N feeding

%Nend = (%15N-excessdigesta / %15N-excessplasma) x 100 Dr. P. Janczyk Bristol 13-14.12.07

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Calculation of plasma 15N-enrichment curves using nonlinear regression model: y=b+a*exp(-c*x) Modell:v4=b+c*exp(-c*v3) y=(,162253)+(,065224)*exp(-(,065224)*x) 0,42 0,40 0,38 0,36 0,34 0,32

anim1

0,30 0,28 0,26 0,24 0,22 0,20 0,18 0,16 0,14 0,12 0,10 -20

0

20

40

60

80

100

time

i=74 Dr. P. Janczyk Bristol 13-14.12.07

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j=98

120

Calculations

Nintake (g/d) – Ndigesta (g/d) CIAPD = Nintake (g/d)

Nintake (g/d) – Ndigesta (g/d) + Nend (g/d) CIRPD =


Nintake (g/d)

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Preliminary results Coefficients of ileal apparent (CIAPD) and real (CIRPD) dietary protein digestibility CIAPD

1

CIRPD

0,75 0,5 0,25 0 RF

A

RF – reference diet Starters: A – RF + C. vulgaris C – RF+ inulin Dr. P. Janczyk Bristol 13-14.12.07

B Starter C

D

B – RF + Na-alginate D – RF + oils 1

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E

E – RF + oils 2

Conclusions

PENS had no negative influence on the apparent and real ileal dietary protein digestibility in weaned pigs

Further analyzes considering the ileal nitrogen composition are to be conducted


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Thank you for your attention


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