Jan 29, 2013 - Enterotoxigenic Escherichia coli (ETEC) infection is common in calves. Egg yolk antibodies (IgY) have been used to treat gastrointestinal ...
Characterization of egg yolk immunoglobulin (IgY) against enterotoxigenic Escherichia coli and evaluation of its effects on bovine intestinal cells Romina Valeria Bellingeri*, Lucila Busso, Fabrisio Eduardo Alustiza, Natalia Yanina Picco, Daniela Paola Molinero, María Carolina Grosso, Carlos Eugenio Motta and Adriana Beatriz Vivas Departamento de Anatomía Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Río Cuarto (5800) Córdoba, Argentina. Accepted 24 January, 2013
Enterotoxigenic Escherichia coli (ETEC) infection is common in calves. Egg yolk antibodies (IgY) have been used to treat gastrointestinal infectious diseases. This study aimed to characterize IgY against bovine ETEC and to evaluate its effects on bovine intestinal cell culture challenged with a bovine ETEC strain. IgY was isolated from the egg yolks of hens immunized with ETEC. The characteristics of IgY were determined by Bradford, ELISA, gel electrophoresis and immunoblotting. Significant differences in anti-ETEC activity between anti-ETEC IgY and non-specific IgY were found in lyophilized fractions. In the bacterial growth assay, anti-ETEC IgY (40 mg/mL) showed growth inhibition of ETEC after 2 h of incubation (p
