ex-vivo: Porcine study comparing prothrombin

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B e b u lin 3 0. 0. 10. 20. 30. 0. 5 0. 100. 150. 200. 250. 300. Bebulin + Hepzyme. Profilnine + Hepzyme. Minutes n. M. T h ro m b in. High Dabigatran. P ro filn in e ...
Honickel et al.

Reversal of Dabigatran

Supplementary Material to Honickel et al. “Reversal of dabigatran anticoagulation

ex-vivo: Porcine study comparing prothrombin complex concentrates and idarucizumab” (Thromb Haemost 2015; 113.4)

Post Trauma

High Dabigatran

Low Dabigatran

H ig h D a b ig a t r a n

P o s t T ra u m a

L o w D a b ig a t r a n

n M T h r o m b in

n M T h r o m b in

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0 0

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B e r ip le x 3 0 C o fa c t + H e p z y m e

C o fa c t 3 0

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C o fa c t 3 0

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150 100

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0 20

n M T h r o m b in

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n M T h r o m b in

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B e rip le x + H e p z y m e P o s t T ra u m a

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M in u t e s

20

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M in u te s

P r o th r o m p le x 3 0

P r o th ro m p le x + H e p z y m e

O c ta p le x 3 0

O c ta p le x + H e p z y m e

30

C o fa c t + H e p z y m e

B e r ip le x 3 0

C o fa c t + H e p z y m e H ig h D a b ig a t r a n

300

10

20

M in u t e s

B e rip le x + H e p z y m e

C o fa c t 3 0

L o w D a b ig a t r a n B e rip le x + H e p z y m e B e r ip le x 3 0

0

10

M in u te s

M in u t e s

n M T h r o m b in

200

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30

M in u te s

P ro th r o m p le x 3 0

P r o th r o m p le x + H e p z y m e

P r o th r o m p le x 3 0

O c ta p le x 3 0

O c ta p le x + H e p z y m e

O c ta p le x 3 0

P r o th ro m p le x + H e p z y m e O c ta p le x + H e p z y m e P o st T rau m a

H ig h D a b ig a t r a n 300

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M in u te s

M in u t e s

M in u t e s B e b u lin 3 0

B e b u lin + H e p z y m e

B e b u lin 3 0

B e b u lin + H e p z y m e

B e b u lin 3 0

B e b u lin + H e p z y m e

P r o f iln in e 3 0

P r o filn in e + H e p z y m e

P r o f iln in e 3 0

P r o filn in e + H e p z y m e

P r o f iln in e 3 0

P r o filn in e + H e p z y m e

Figure 6

Suppl. Figure 1: Representative curves of plasma thrombin generation before and after the addition of heparinase. Dabigatran dose-dependently inhibited thrombin generation. By adding three-factor PCCs and Beriplex or Cofact, dabigatran-induced inhibition of thrombin generation could be reversed. In contrast, Prothromplex and Octaplex only reversed thrombin generation after addition of heparinase. Therefore, thrombin generation was measured after the addition of heparinase to all samples. Representative curves are shown for “low dabigatran”, “high dabigatran” and “post trauma”.