Experimental proposal - arXiv

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H. Kraus. 2. 1. Diamond Light Source, Harwell Campus, Didcot, OX11 0DE, UK. 2 ...... [18] V. B. Mikhailik, H. Kraus, S. Henry and A. J. B. Tolhurst, , “Scintillation ...
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Non-contact luminescence lifetime cryothermometry for macromolecular crystallography V. B. Mykhaylyk1*, A. Wagner1, H. Kraus2 1

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Diamond Light Source, Harwell Campus, Didcot, OX11 0DE, UK University of Oxford, Department of Physics, Denys Wilkinson Building, Keble Road, Oxford, OX1 3RH, UK

Abstract Temperature is a very important parameter when aiming to minimize radiation damage to biological samples during experiments that utilise intense ionising radiation. A novel technique for remote, non-contact, in situ monitoring of the protein crystal temperature has been developed for the new I23 beamline at the Diamond Light Source, a facility dedicated to macromolecular crystallography (MX) with long-wavelength X-rays. The temperature is derived from the temperature-dependant decay time constant of luminescence from a minuscule scintillation sensor (