Exploring passive clearing for 3D optical imaging of nanoparticles in intact tissues Shrey Sindhwani,1† Abdullah Muhammad Syed,1† Stefan Wilhelm,1 Warren C. W. Chan1,2, 3, 4, 5,*
Affiliations 1
Institute of Biomaterials and Biomedical Engineering, Rosebrugh Building, Room 407, 164 College Street, Toronto, Ontario M5S 3G9, Canada
2
Department of Chemistry, 80 St George Street, Toronto, Ontario M5S 3H6, Canada
3
Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Room 230, Toronto, Ontario M5S 3E1, Canada
4
Department of Chemical Engineering, 200 College Street, Toronto, Ontario M5S 3E5, Canada
5
Department of Material Science and Engineering, 160 College Street, Room 450, University of Toronto, Toronto M5S 3E1, Canada *: Correspondence to Warren C. W. Chan (
[email protected]) †: These authors contributed equally to this work.
Table of Contents Figure S1: Liver, kidney and brain tissues clear at varying rates as measured in SDS but achieve similar transparency after refractive index matching. ....................................................................................................... 3 Figure S2: Image Quality Score (BRISQUE) as a function of imaging depth in a liver tissue containing QDs. ........... 4
Figure S1: Liver, kidney and brain tissues clear at varying rates as measured in SDS but achieve similar transparency after refractive index matching. a) Optical density measured at 647 nm decreased more rapidly for brain hemisphere than for liver and kidneys when cleared at 37°C passively. b) After 28 days of clearing, refractive index matching of liver, kidney and brain tissues achieved similar levels of transparency. SDS: sodium dodecyl sulfate. RIMS: refractive index matching solution (88% Iohexol, full recipe in methods). All points indicate mean ± standard deviation for 3 replicates.
Figure S2: Image Quality Score (BRISQUE) as a function of imaging depth in a liver tissue containing QDs. Over 500 µm there is no loss of image quality in a liver tissue cleared for 6 days with passive clearing (50 °C).
