S2 Figure. Colocalization analyses of mCh-ChsB and GFP-TlgB in a hypB5 (sec7 ) strain. Pictures taken at different times after shifting cells at 37ºC, with ...
TlgBTlg2
ChsB
ChsB
ChsB TlgBTlg2
TlgBTlg2
TlgBTlg2
normalized signal intensity (ChsB channel)
normalized signal intensity (ChsB channel)
ChsB
1.0
0.5
2 mm
-0.1
2 mm
hypB5 14 min, 37ºC
0.0
0.1 0.2 (Ai-a)(Bi-B)
0.3
hypB5, 24 min 37ºC
Pearson's co. = 0.47 Li's ICQ = 0.218
-0.2
0.0
0.2
ChsB TlgBTlg2
normalized signal intensity (ChsB channel)
ChsB TlgBTlg2
1.0
0.5
2 mm
2 mm
Pearson's co. = 0.63 -0.1 Li's ICQ = 0.287
0.0
0.1 0.2 (Ai-a)(Bi-B)
1.0
0.5
-0.1
0.3
Pearson's co. = 0.74 Li's ICQ = 0.27
hypB5, 38 min 37ºC
hypB5, 27 min 37ºC
ChsB
ChsB TlgBTlg2
0.0
0.1 0.2 (Ai-a)(Bi-B)
TlgBTlg2
2 mm
normalized signal intensity (ChsB channel)
TlgBTlg2
ChsB TlgBTlg2
ChsB
0.4
(Ai-a)(Bi-B)
hypB5, 26 min 37ºC normalized signal intensity (ChsB channel)(ChsB channel)
TlgBTlg2
ChsB
0.5
Pearson's co. = 0.66 Li's ICQ = 0.253
hypB5, 26 min, 37ºC ChsB TlgBTlg2
1.0
-0.1
1.0
0.5
0.0
0.1
0.2
0.3
(Ai-a)(Bi-B)
normalized signal intensity (ChsB channel)
Pearson's co. = 0.75 Li's ICQ = 0.317
2 mm
-0.1
1.0
0.5
Pearson's co. = 0.62 Li's ICQ = 0.251 0.0
0.1
0.2
0.3
(Ai-a)(Bi-B)
S2 Figure. Colocalization analyses of mCh-ChsB and GFP-TlgBTlg2 in a hypB5 (sec7ts) strain Pictures taken at different times after shifting cells at 37ºC, with indication of Pearson's and Li's colocalization coefficients. Plots were used to calculate Li's ICQ.
