a) Overlap in genes differentially regulated versus control in CSE-treated and ... 17/127. -1.78. Focal adhesion. 19/202. -1.77. Metabolic pathways. 66/1239.
Figure S1
Fold change Senescence vs. Control
Senescence fold changes -10 0 RNA-seq PCR RNA-seq
+10
RNA-seq
PCR
ALDH3A1
►
►
42.5
38.5
4.9
AKR1C1
►
►
19.4
11.5
4.7
AKR1B10
►
►
16.9
20.6
►
9.6
11.5
GPX2
8.7
13.4
AKR1C3
8.0
9.4
EPHX1
3.8
4.8
OSGIN1
3.7
5.3
ALDH1A3
3.3
4.3
ALDH3A2
2.8
2.5
5.8
7.7
AOX1
3.4 2.9
ALDH1A3
2.8
CSE fold changes RNA-seq PCR
PCR
EPHX4 SOD2
CSE vs. Control
2.9
AKR1C2
XDH
2.3
2.6
ALDH18A1
1.8
1.9
SDR42E1
-2.0
-1.8
AKR1C2
-2.3
-1.7
GSTO2
-2.5
-3.0
AKR1B10
-3.0
-5.1
PRDX1
2.7
3.5
ALDH1L2
-3.2
-3.5
MGST1
2.7
2.2
ADHFE1
-4.4
-6.1
GSTA4
2.5
2.4
ALDH3B2
-6.2
-9.1
AKR1B1
2.0
2.1
-19.5
ALDH1L1
-4.9
-3.7
-15.3
ALDH1L2
-5.2
-5.9
ALDH1A1
►
-7.4
ALDH1L1
►
-24.8
►
Figure S1. Validation of RNA-seq expression fold changes. Comparison of expression fold-changes by RNA-seq and quantitative real-time PCR for genes with significant (FDR < 0.05) changes in expression upon replicative senescence (left) or CSE exposure (right). Bars reflect relative fold changes from control to experimental conditions, up to ±10-fold (with greater changes indicated by ‘►’). Dashed lines connect genes with significant expression changes in both conditions.