Figure S1

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a) Overlap in genes differentially regulated versus control in CSE-treated and ... 17/127. -1.78. Focal adhesion. 19/202. -1.77. Metabolic pathways. 66/1239.
Figure S1

Fold change Senescence vs. Control

Senescence fold changes -10 0 RNA-seq PCR RNA-seq

+10

RNA-seq

PCR

ALDH3A1





42.5

38.5

4.9

AKR1C1





19.4

11.5

4.7

AKR1B10





16.9

20.6



9.6

11.5

GPX2

8.7

13.4

AKR1C3

8.0

9.4

EPHX1

3.8

4.8

OSGIN1

3.7

5.3

ALDH1A3

3.3

4.3

ALDH3A2

2.8

2.5

5.8

7.7

AOX1

3.4 2.9

ALDH1A3

2.8

CSE fold changes RNA-seq PCR

PCR

EPHX4 SOD2

CSE vs. Control

2.9

AKR1C2

XDH

2.3

2.6

ALDH18A1

1.8

1.9

SDR42E1

-2.0

-1.8

AKR1C2

-2.3

-1.7

GSTO2

-2.5

-3.0

AKR1B10

-3.0

-5.1

PRDX1

2.7

3.5

ALDH1L2

-3.2

-3.5

MGST1

2.7

2.2

ADHFE1

-4.4

-6.1

GSTA4

2.5

2.4

ALDH3B2

-6.2

-9.1

AKR1B1

2.0

2.1

-19.5

ALDH1L1

-4.9

-3.7

-15.3

ALDH1L2

-5.2

-5.9

ALDH1A1



-7.4

ALDH1L1



-24.8



Figure S1. Validation of RNA-seq expression fold changes. Comparison of expression fold-changes by RNA-seq and quantitative real-time PCR for genes with significant (FDR < 0.05) changes in expression upon replicative senescence (left) or CSE exposure (right). Bars reflect relative fold changes from control to experimental conditions, up to ±10-fold (with greater changes indicated by ‘►’). Dashed lines connect genes with significant expression changes in both conditions.

Figure S2 b

Senescent CSE

Senescent Down

1291 243 41% CSE

356

Both up

638 25 14%

297

CSE down Sen. up

835

599

42 22%

CSE

CSE up Sen. down

Senescent

c

164

Down

Up

Down

115 28%

115

64

Up

762

147

Up

Up

Both down

Neither

Down

All FDR