figures JASN supp data Lenoir et al.pptx

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Supplemental figure 1: Immunohistological characterization of nephrin and podocin expression in primary podocyte culture. (A) Representative image of nephrin ...

A

nephrin / DAPI

B

podocin / DAPI

Supplemental figure 1: Immunohistological characterization of nephrin and podocin expression in primary podocyte culture. (A) Representative image of nephrin expression (green) in primary podocytes isolated from WT mice. (B) Representative image of podocin expression (green) in primary podocytes isolated from WT mice. Nuclei were counterstained with DAPI stain (blue). Adherent glomeruli are indicated by arrows. Scale bar 200μm.

Fold change in fluorescence intensity (% of ET1 stimulated cells)

*

120 100 80

***

60 40 20 0

ET1!

BQ-123! BQ-788!

+ -

+ + -

+ +

+ + +

Supplemental figure 2: Activation of the Ca2+ pathway in podocytes stimulated by ET-1 in the presence of ETAR antagonist, BQ-123, or ETBR antagonist, BQ-788. Primary podocytes were stimulated with ET-1 (1μM) in the presence of BQ-123 (1μM), BQ-788 (1μM) or both BQ-123+BQ-788 and maximal fluorescence ratios are measured. Values are mean ± sem of three independent experiments. * p