File S4. - PLOS

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TPChic0613. SufD protein. FeS assembly. TPChic0079. Putative aldehyde oxidase and xanthine dehydrogenase family protein. Oxidative metabolism of purines.
File S4. Hypothetical new protein function Locus Tag Product (Chicago) TPChic0017 Tetratricopeptide repeat (TPR) domain protein TPChic0024 TrkA domain protein TPChic0025

Possible function or cellular process Unknown

Locus Tag (Chicago) TPChic0502

Product

Tyrosine kinase

TPChic0512

Metalloprotease

TPChic0515

Methyltransferase

TPChic0518

Organic solvent tolerance protein Thiamine pyrophosphokinase

TPChic0522

CvpA family protein

TPChic0544

Putative ankyrin repeatcontaining protein Bifunctional enzyme IspD/IspF

Possible function or cellular process Regulation of antioxidation metabolism MEP cytidylyltransferase/ MECDP-synthase Sugar transporter of phosphotransferase system Thiamine pyrophosphate synthesis Colicin V production

TPChic0034

M16 peptidase family protein rRNA small subunit methyltransferase Mzt family protein

TPChic0035

ATP-binding protein

Manganese/zinc/iron ABC transporter, substratebinding protein Metal transport

TPChic0036

Membrane protein

Metal transport

TPChic0559

Endonuclease/ exonuclease/ phosphatase family ThiI protein

TPChic0042

Putative LysM domain protein Tpl protein

Glucan-binding protein/ glucosyltransferase Signal peptidase II-like activity Purine salvage

TPChic0563

DnaJ domain-containing protein

Thiamine biosynthesis/tRNA modification protein Chaperone

TPChic0564

Putative membrane protein

Unknown

TPChic0572

Unknown

Methyltransferase RNA methyltransferase Na+ transport across membranes

TPChic0580 TPChic0582 TPChic0587

Putative FMN-binding domain protein Lipoprotein releasing system Lipoprotein releasing system HolA protein

Unknown

TPChic0601

Dxr protein

1-deoxy-D-xylulose 5phosphate reductoisomerase Di-trans,poly-cisdecaprenyl cistransferase FeS assembly

TPChic0032

TPChic0046 TPChic0050 TPChic0052 TPChic0054 TPChic0055

TPChic0067

Phosphoribosyl transferase domain protein PrmC protein TrmH family protein Sodium pump decarboxylases, gamma subunit Tetratricopeptide repeat (TPR) domain protein

TPChic0068

Cfr family protein

Generation of catalytic radicals

TPChic0603

UppS protein

TPChic0069

Putative lipoprotein

Unknown

TPChic0612

SufB protein

Intracellular signaling

Permease Permease DNA polymerase III, delta subunit

TPChic0073

HD domain protein

TPChic0079

TPChic0086

Putative aldehyde oxidase and xanthine dehydrogenase family protein Putative FAD-binding protein PilZ protein

TPChic0087

Putative lipoprotein

TPChic0095

Tetratricopeptide repeat (TPR) domain protein MetN protein

TPChic0081

TPChic0120 TPChic0121

Metal-dependent phosphohydrolases Oxidative metabolism of purines

TPChic0613

SufD protein

FeS assembly

TPChic0622

Tetratricopeptide repeat (TPR) domain protein

Unknown

Unknown

TPChic0624

OmpA family protein

Porin

Type IV pilus assembly protein Unknown

TPChic0625

Putative lipoprotein

Unknown

TPChic0628

Unknown

Unknown

TPChic0636

Putative nicotinate phosphoribosyl transferase RecO protein

Methionine import ATPbinding protein Generation of catalytic radicals Unknown

TPChic0646

Putative lipoprotein

Unknown

TPChic0648

Unknown

TPChic0650

Tetratricopeptide repeat (TPR) domain protein Putative metalloprotease

Tyrosine kinase

TPChic0668

Putative yggt family

Repeat found in conserved hypothetical integral membrane proteins Endonuclease

DNA repair

TPChic0139

Putative radical SAM domain protein Tetratricopeptide repeat (TPR) domain protein TrkA domain protein

TPChic0148

Putative membrane protein

Unknown

TPChic0674

TPChic0149

Putative lipoprotein

Unknown

TPChic0691

Putative Smr domain-containing protein ScpA/B protein

TPChic0153

PAP2 family protein

TPChic0693

Putative lipoprotein

TPChic0154

Putative S4 domain protein

TPChic0702

TPChic0156

Putative M23 peptidase domain protein Thioesterase family protein

TPChic0710

TPChic0158

HAD-superfamily hydrolase

Non ribosomal protein synthesis Haloacid dehalogenase-like hydrolase

M23/M37 domain-containing protein M23/M37 domain-containing protein Putative polymerase

Peptidase

TPChic0155

Type 2 phosphatidic acid phosphatase Voltage-gated proton channels Beta-lytic metallopeptidase

TPChic0719

Putative FliO protein

Flagellum biosynthesis

TPChic0123

2

TPChic0700

Unknown

Segregation and condensation protein Unknown

Peptidase Unknown

TPChic0181

Putative septum formation initiator subfamily Putative YrdC domain

Unknown

TPChic0730

CDP superfamily protein

rRNA maturation factor

TPChic0740

HD domain-containing protein

TPChic0231

RNA pseudouridine synthase superfamily

Formation of pseudouridine in 16S rRNA

TPChic0741

NadD protein

TPChic0256

Putative von Willebrand factor type A domain Putative LysM domain protein Putative lipoprotein

Unknown

TPChic0754

MiaB protein

Glucan-binding proteins and glucosyltransferases Unknown

TPChic0764

HD domain-containing protein

TPChic0771

Tetratricopeptide repeat (TPR) domain protein Tetratricopeptide repeat (TPR) domain protein Cytidylyltransferase domain protein HAD-superfamily hydrolase, subfamily IIB FMN-dependent dehydrogenase superfamily Dephospho-CoA kinase

Unknown

TPChic0796

Na+/inorganic phosphate (Pi) cotransporter family protein ApbE lipoprotein

Unknown

TPChic0802

DHH superfamily protein

Phosphoesterase

Aminophosphonate metabolism. Haloacid dehalogenase-like hydrolase Hydroxyacid oxidase

TPChic0815

GNAT family protein

Acetyltransferase

TPChic0819

RnZ protein

Ribonuclease

TPChic0820

Unknown

Pantothenate and CoA biosynthesis.

TPChic0822

Tetratricopeptide repeat (TPR) domain protein Mechanosensitive ion channel family protein

Permease protein

TPChic0839

Putative Lipoprotein

Permease protein

TPChic0840

Penicillin-binding protein

TPChic0854

Unknown

TPChic0864

TPChic0333

LolA protein

TPChic0875

TPChic0335

CAAX amino protease

Outer membrane lipoprotein carrier Amino terminal protease

Putative transporter, major facilitator family HAMP domain-containing protein LysM/M23/M37 domaincontaining protein ATP-binding protein

Unknown

TPChic0312

Putative amino acid or sugar ABC transport systems putative amino acid or sugar ABC transport systems Pasta domain-containing protein Putative membrane protein

TPChic0876

YeaZ protein

Protease

TPChic0182

TPChic0259 TPChic0260 TPChic0268 TPChic0282 TPChic0288 TPChic0290 TPChic0291 TPChic0296

TPChic0301 TPChic0302 TPChic0307

3

Alcohol phosphatidyltransferase Metal-dependent phosphohydrolases Nicotinate (nicotinamide) nucleotide adenylyltransferase tRNA-I(6)A37 thiotransferase Metal-dependent phosphohydrolases Ion transport Thiamine biosynthesis

Response to stretching of the cell membrane and to membrane depolarization Unknown

Cell signaling Peptidase Unknown

TPChic0358

family Glycosyl hydrolase

TPChic0371

IspE protein

TPChic0373 TPChic0381 TPChic0383

tRNA(Ile)-lysidine synthase Integral membrane protein MraZ protein

TPChic0384

MraW protein

TPChic0385 TPChic0392

FtsL protein Tetratricopeptide repeat (TPR) domain protein SurE protein

TPChic0419 TPChic0421 TPChic0431 TPChic0436 TPChic0438 TPChic0441

Tetratricopeptide repeat (TPR) domain protein Type III pantothenate kinase DHH superfamily protein, subfamily 1 RdgB/HAM1 family protein

TPChic0449

Probable inorganic polyphosphate/ATP-NAD kinase Putative LysM domain protein Putative lipoprotein

TPChic0456 TPChic0458

Putative lipoprotein ScpB protein

TPChic0459

RNA pseudouridine synthase superfamily

TPChic0444

Synthesis and breakage of glycosidic bonds. 4-(cytidine 5'-diphospho)-2C-methyl-D-erythritol kinase

TPChic0877

HD domain-containing protein

TPChic0883

YjgP/YjgQ family protein

Metal-dependent phosphohydrolases Permease

TPChic0884 TPChic0901 TPChic0906

YjgP/YjgQ family protein MATE family protein KH domain-containing protein

Permease Efflux pump RNA processing

TPChic0907

RimM protein

16S rRNA processing protein

TPChic0911 TPChic0912

FlhB domain protein HD domain-containing protein

5'-nucleotidase

TPChic0915

Unknown

TPChic0920

Pantothenate and CoA biosynthesis. Phosphoesterase

TPChic0937

RdgB non-canonical purine NTP pyrophosphatase Phosphorylation of NAD

TPChic0949

Tetratricopeptide repeat (TPR) domain protein Tetratricopeptide repeat (TPR) domain protein Phosphodiesterase family protein Tetratricopeptide repeat (TPR) domain protein Inner membrane protein OxaA

TPChic0954

Tetratricopeptide repeat (TPR) domain protein

Glucan-binding proteins and glucosyltransferases Unknown

TPChic0962

Putative efflux ABC transporter

Permease

TPChic0965

Efflux transporter

Unknown Segregation and condensation protein Formation of pseudouridine in 16S rRNA

TPChic0972 TPChic0975

RND family protein, MFP subunit FTR1 family protein Tetrapyrrole methylase family protein TatD family protein

Unknown Cell-wall biosynthesis and cell division S-adenosylmethyltransferase Cell division Unknown

TPChic0944

4

TPChic0979

Metal-dependent phosphohydrolases Unknown Unknown Unknown Unknown Integration of membrane proteins Unknown

Iron permease Unknown Hydrolase

TPChic0460 TPChic0464

Tetratricopeptide repeat (TPR) domain protein TrmB protein

TPChic0465

Putative lipoprotein

TPChic0470

Tetratricopeptide repeat (TPR) domain protein Tetratricopeptide repeat (TPR) domain protein Putative membrane protein

Unknown

TPChic0994

Multiple antibiotic resistance protein Tetratricopeptide repeat (TPR) domain protein TatD family protein

Unknown

TPChic1003

Putative membrane protein

Unknown

Unknown

TPChic1018

Similar to protein Ppro_2673 Metallo-beta-lactamase family protein Tetratricopeptide repeat (TPR) domain protein

Unknown

TPChic1029

2',3'-cyclic-nucleotide 2'phosphodiesterase DbpA protein

Cell wall synthesis

TPChic1033

Patatin family protein

Purine and pyrimidine metabolism RNA-binding domaincontaining protein Phospholipase

Unknown

TPChic1034

putative K+-dependent Na+/Ca+ exchanger homolog

TPChic0471 TPChic0473 TPChic0474 TPChic0489 TPChic0496

Unknown

TPChic0986

DUF6 protein

tRNA (guanine-N(7)-)methyltransferase Unknown

TPChic0988 TPChic0990

5

Putative integral membrane protein Efflux pump Unknown Hydrolase

Ion transport