Final Report - PIRSA

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Final Report

AQUAFIN CRC – FRDC SOUTHERN BLUEFIN TUNA AQUACULTURE SUBPROGRAM: COORDINATION, FACILITATION AND ADMINISTRATION Steven Clarke and Jane Ham

February 2009 Aquafin CRC Project 5B.1(2) (FRDC Project No. 2004/216)

National Library of Australia Cataloguing-in-Publication Entry Clarke S and Ham J (2009). Aquafin CRC – Southern Bluefin Tuna Aquaculture Subprogram: coodination, administration and facilitation. FRDC Final Report (Project No. 2004/216). SARDI Aquatic Sciences. SARDI Research report series No. 329, 78pp.

© South Australian Research and Development Institute, Aquafin CRC and FRDC This work is copyright. Except as permitted under the Copyright Act 1968 (Cth), no part of this publication may be reproduced by any process, electronic or otherwise, without the specific written permission of the copyright owners. Neither may information be stored electronically in any form whatsoever without such permission. Every attempt has been made to provide accurate information in this document. However, no liability attaches to Aquafin CRC, its Participant organisations or any other organisation or individual concerned with the supply of information or preparation of this document for any consequences of using the information contained in the document.

ISBN 978-1-921563-07-2 SARDI Research report series No. 329 SARDI Aquatic Sciences Publication No. F2007/000876-2 Printed in February 2009 by: PIRSA Customer Services 101 Grenfell St Adelaide SA 5001 Authors: Steven Clarke and Jane Ham Reviewers: Peter Montague and Craig Hayward Approved by: Jason Tanner

Date: 3 February 2009 Distribution: FRDC, FRAB representatives, libraries, scientific and industry contributors. Circulation: Public domain

AQUAFIN CRC – FRDC SOUTHERN BLUEFIN TUNA AQUACULTURE SUBPROGRAM: COORDINATION, FACILITATION AND ADMINISTRATION Steven Clarke and Jane Ham February 2009 Aquafin CRC Project 5B.1(2) (FRDC Project No. 2004/216)

TABLE OF CONTENTS NON-TECHNICAL SUMMARY .................................................................................1 ACKNOWLEDGEMENTS...........................................................................................4 BACKGROUND ...........................................................................................................5 SBT Aquaculture Industry .........................................................................................5 SBT Aquaculture Subprogram...................................................................................5 NEED.............................................................................................................................6 OBJECTIVES ................................................................................................................6 METHODS ....................................................................................................................7 Management...............................................................................................................7 Policy Development...................................................................................................7 R&D Priority Setting and New Project Assessment..................................................7 Infrastructure..............................................................................................................8 Operational Plans and Budgets ..................................................................................8 Budget Reporting .......................................................................................................8 Communication and Technology Transfer ................................................................9 Strategic Planning ....................................................................................................10 RESULTS AND DISCUSSION ..................................................................................10 Industry Development..............................................................................................10 Research Funding.....................................................................................................11 Strategic Planning ....................................................................................................12 Projects.....................................................................................................................17 OUTPUTS....................................................................................................................19 2004..........................................................................................................................19 2005..........................................................................................................................22 2006..........................................................................................................................24 2007..........................................................................................................................25 2008..........................................................................................................................27 Education and Training............................................................................................28 Publications (2004-2008).........................................................................................32 SBT Aquaculture Subprogram Website...................................................................57 BENEFITS...................................................................................................................58 FURTHER DEVELOPMENT.....................................................................................59 PLANNED OUTCOMES............................................................................................59 CONCLUSIONS..........................................................................................................60 REFERENCES ............................................................................................................61 Appendix 1 – Intellectual Property (IP)...................................................................62 Appendix 2 – Staff ...................................................................................................62 Appendix 3 – Project Outcomes and Outputs..........................................................62

NON-TECHNICAL SUMMARY 2004/216

Aquafin CRC – FRDC SBT Aquaculture Subprogram: coordination, administration and facilitation

PRINCIPAL INVESTIGATOR:

Steven Clarke

CO-INVESTIGATOR:

Jane Ham

ADDRESS:

South Australian Research and Development Institute C/- PO Box 120 Henley Beach SA 5022 Telephone: (08) 8207 5443 Fax: (08) 8207 5481

OBJECTIVES: The FRDC Southern Bluefin Tuna (SBT) Aquaculture Subprogram had as its objectives: 1. Strategic Planning: provide a coordinated research and development program addressing the focus areas of the Strategic R&D Plan: 2001-2006 for the SBT Aquaculture Industry and the specified R&D objectives and outcomes of the Aquafin CRC Commonwealth Agreement. Review and update the R&D Plan on an annual basis. 2. Administration: coordinate and manage project, subprogram and program milestones, variations, budgets, communications and meetings relevant to the SBT (southern bluefin tuna) Aquaculture Subprogram. 3. Information Technology Transfer: disseminate information to facilitate the uptake and commercialisation of research outcomes via “Tuna-brief” newsletters, an annual industry conference (including published Conference Proceedings), industry workshops, meetings with individual companies and a regularly updated website.

PLANNED OUTCOMES: The planned outcomes of the SBT Aquaculture Subprogram were: 1. An orderly and structured approach to research and development associated with SBT aquaculture meeting the needs of the Industry and research managers/funders (measured by the level of Industry, Aquafin CRC and FRDC satisfaction in the Subprogram). 2. An up-to-date R&D Plan meeting the needs of Industry participants (measured by the number and success of new research applications).

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3. Efficient management and administration of the Subprogram achieving the specified milestones, outcomes and outputs (measured by the timeliness of milestone and final reports). 4. Efficient and coordinated use of the limited resources available for SBT aquaculture related research and development (measured by the diversity, suitability and satisfaction with the research platforms in use). 5. Appropriately managed communications disseminating research results (measured by the level of communication and absence of negative media issues).

ACHIEVEMENTS: This project implemented the agreed Strategic R&D Plan for the SBT aquaculture industry for the period of 2004 – 2008 and in doing this provided the key linkage with the Fisheries Research and Development Corporation and the Aquafin CRC. It provided an administrative framework and processes that: • engaged stakeholders, specifically the SBT aquaculture industry and the State managers responsible for its development and regulation, in the process of identifying and prioritising research needs and monitoring the projects designed to meet those needs; • enabled research groups to shape their programs to the needs of industry, government and other stakeholders; • maximised and coordinated the use of the limited research infrastructure available; • led to the development of additional research infrastructure, equipment and people capability; and • maximised the transfer of useful information from research projects to endusers. As a consequence, all the projects undertaken within the SBT Aquaculture Subprogram research portfolio were directed at outcomes strongly supported by endusers. The projects all involved a high level of active collaboration between researchers and with the SBT industry, and adoption of the successful research outcomes was rapid. As measured by the Aquafin CRC, the impact of a wide range of research outcomes has already been substantial. Key outcomes of the SBT Aquaculture Subprogram have been: • • • • •

facilitation of the development and distribution of a new SBT Aquaculture Strategic R&D Plan for the Industry; continuing strong involvement in the Aquafin CRC; the development, initiation and management of a wide range of research projects; further development, training and education of industry and research personnel; publication and distribution of considerable extension material to industry; and

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further development of the SBT Aquaculture Subprogram password protected website to act as a comprehensive depository of information for the industry.

The SBT Aquaculture Subprogram has over the current and past project (FRDC Project No. 2001/250) addressed the challenges that have arisen, and successfully delivered the desired outcomes (Clarke et al, 2005). The Subprogram over the past 4 years managed 23 projects worth about $9.44 million (Aquafin CRC and FRDC funds, not including research organisation and industry in-kind; Aquafin CRC figures), building on the portfolio of the previous Subprogram.

KEYWORDS: Southern bluefin tuna, Aquaculture, SBT Aquaculture Subprogram, South Australia, Aquafin CRC, and FRDC.

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ACKNOWLEDGEMENTS We wish to gratefully acknowledge all of the people who have contributed to the success of the SBT Aquaculture Subprogram. In particular: Steering Committee: Rob Lewis (Chair), Richard Stevens (previous Chair), Peter Montague, Patrick Hone, Brian Jeffriess, David Ellis, Daryl Evans, David Warland (previous member), Justin Nelligan (previous member), Ian Nightingale, Pauline Mooney, Mehdi Doroudi, Barbara Nowak, John Volkman, Chris Carter and Anthony Cheshire (previous member). Scientific participants (including from the Australian Southern Bluefin Tuna Industry Association - ASBTIA): Jeff Buchanan, Robert van Barneveld, Philip Thomas, Kathy Schuller, Trent D’Antignana, Mark Thomas, Roger Seymour, Quinn Fitzgibbon, Andrew Pointon, John Carragher, Richard Musgrove, David Padula, Ben Daughtry, Kathy Ophel-Keller, Nathan Bott, Craig Hayward, Hamish Aiken, Jason Tanner, Maylene Loo, Milena Fernandes, Sam Mc Clatchie, John Middelton, Pru Bonham, Lesley Clementson, Mike Hertzfeld, Nugar Margvelishvilli, Peter Thompson, Karen Wild-Allen, Kirsten Rough and Danielle Foote. And the SBT farming companies and their employees: Ajka Pty Ltd, Australian Fishing Enterprises Pty Ltd, Australian Tuna Fisheries Pty Ltd, Blaslov Fishing Pty Ltd, Eyre Tuna Pty Ltd, Emily Kristina (Aust) Pty Ltd, Marnikol Fisheries Pty Ltd, KIS Tuna Pty Ltd, Sarin Marine Farm Pty Ltd, Sekol and Sams Tuna Pty Ltd.

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BACKGROUND SBT Aquaculture Industry The aquaculture of southern bluefin tuna (SBT) commenced in 1990 and over the last 18 years has expanded rapidly, today producing about 7500 tonnes worth about $138 million (2006-07 production figures; ABARE, 2008). In recent years the industry has been challenged by increased production of northern bluefin tuna in the Mediterranean and Mexico, and the appreciation of the Australian dollar against the Japanese Yen, causing the value of SBT farming to drop from its peak of $311 million in 2000-01 (ABARE, 2008) to its current value. Despite this, the SBT industry in 2006-07 is the third largest export orientated Australian fishing industry (excluding pearls), with most of its value coming from aquaculture (ABARE, 2008). It has also had a significant impact on the Eyre Peninsula region, directly and indirectly employing around 1200 people, generating a total of $278.3 million (indirect and flow on effects) in the region (Econosearch, 2007). Despite SBT aquaculture now including most of the Australian SBT quota, opportunity exists for continued industry growth in sales volume from the improved survival of farmed SBT; the farming of SBT for longer periods of time; and the potential farming of overseas based quota and the aquaculture of hatchery produced SBT. Improvements in product quality and advances in feeds, feeding strategies and farming technology have the opportunity to lead to better market prices and reduced operating costs, respectively. The SBT Aquaculture Subprogram over the past 4 years sought to optimise the position of the SBT Aquaculture Industry with respect to these trends through well prioritised and coordinated research and development, and in doing so to address the key issues that maintain or enhance the Industry's competitiveness. SBT Aquaculture Subprogram The SBT Aquaculture Subprogram was formed by FRDC in 1997 to manage research addressing this industry's key research and development priorities and in doing so increase industry’s ownership of the processes involved. The structure and resources used reflected best practice based on some of the other FRDC Subprograms at the time: a Steering Committee with independent chairperson, Communications Subcommittee, Scientific Advisory Committee, and Program Leader supported by a part-time Subprogram Project Officer. Primary sources of communication to industry were through working closely with representatives of the SBT industry association (previously the Tuna Boat Owners Association of Australia - TBOASA and more recently the Australian Southern Bluefin Tuna Industry Association – ASBTIA); by way of the "Tuna-brief', a dedicated newsletter; an annual industry conference; R&D planning and technical workshops; and through direct communication with industry members and companies. Another important source of communication was the SBT Aquaculture Subprogram

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website that has a public area, and two password protected sections, one for industry members and the other for the Steering Committee.

NEED This project was essential for the development of a cohesive research and development approach aimed at meeting the priority needs of the SBT Aquaculture Industry. The industry had developed rapidly since its initiation in 1990 and had the opportunity to continue to do so; however targeted research and development was needed to underpin this development and to assure the long-term sustainability of the industry in an increasingly competitive international market. This project provided the basis for the continuation and further development of the SBT Aquaculture Subprogram and its objectives, as well as ensuring that appropriate inputs and outcomes were delivered to the Aquafin CRC (the SBT Industry was a key participant and the Production and Value Adding Programs were led by the Subprogram Leader). Support of the SBT Aquaculture Subprogram provided the opportunity for an ongoing management structure to oversee the active research projects and ensure that the desired outcomes could be achieved; provide a focused strategy for disseminating research results to industry and obtaining feedback; and setting research priorities. In doing this it also had the opportunity to ensure that the minimum level of duplication occurred in the provision of research services; provide a focus for developing SBT aquaculture related research infrastructure and people; address industry priorities by establishing a mechanism to empower industry's involvement in their research; establish a framework to ensure that SBT aquaculture research is orderly and targeted; and disseminate, where appropriate, research information to stakeholders. The cost of the Subprogram against research funds over the 4 year period was approximately 4.62% (not including In Kind funds). The subprogram throughout this period also supported a number of additional projects, such as the development of an online SBT Industry Micro-algal Database and supporting Real-time environmental data coming from the telemetry system located in the Boston Island SBT Farming Zone (and in the future data from another telemetry system in the Rabbit Island Zone) available on the SBT Aquaculture Subprogram website.

OBJECTIVES 1. Strategic Planning: provide a coordinated research and development program addressing the focus areas of the Strategic R&D Plan: 2001-2006 for the SBT Aquaculture Industry and the specified R&D objectives and outcomes of the Aquafin CRC Commonwealth Agreement. Review and update the R&D Plan on an annual basis. 2. Administration: coordinate and manage project, subprogram and program milestones, variations, budgets, communications and meetings relevant to the SBT Aquaculture Subprogram. 3. Information Technology Transfer: disseminate information to facilitate the uptake and commercialisation of research outcomes via “Tuna-brief”

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newsletters, an annual industry conference (including published Conference Proceedings), industry workshops, meetings with individual companies and a regularly updated website.

METHODS The project outlined here focused on the coordination, facilitation and administration of research undertaken within the SBT Aquaculture Subprogram (i.e. by the Aquafin CRC and FRDC). Management The Subprogram was managed by a Steering Committee that obtained advice from the Scientific Advisory Committee as required. Representation on both committees was sector and expertise based, and included membership of the FRDC (Program Manager), Aquafin CRC (CEO, and Program Leaders - observers), ASBTIA (Executive Director, Research Manager and two SBT Farm Managers), Government representatives (Executive Director – PIRSA Aquaculture) and representatives with appropriate scientific expertise (CSIRO or SARDI). The Subprogram Leader provided leadership, coordination and administration functions. Industry provided input to the Subprogram through committee members, involvement in industry workshops and conferences, and direct communication with the Subprogram Leader and Principal Investigators of operational projects. Key Subprogram tasks were to provide direction on industry research and development priorities, and coordinate and manage Aquafin CRC and FRDC grant applications in association with state based FRABs and the FRDC. The Subprogram Leader also liaised with and provided support to relevant companies and researchers seeking alternative funding sources (e.g. AusIndustry) to address industry priorities. The structure, policy and operations of the Subprogram are documented as part of the Southern Bluefin Tuna Aquaculture Strategic R&D Plan – Towards 2012: Striving for a Profitable and Sustainable Future. Policy Development The SBT Aquaculture Subprogram, as the key body coordinating research and development, continued to develop and document the policies agreed upon by the Steering Committee and made these more accessible by providing them on the SBT Aquaculture Subprogram website. The Subprogram also disseminated the relevant policies of the Aquafin CRC and FRDC. A communications policy existed for the Subprogram and was monitored through a Communications Subcommittee. R&D Priority Setting and New Project Assessment The SBT aquaculture industry’s research and development priorities continued to be reviewed annually as part of the June meetings in order for targeted research project proposals to be developed. All farm owners and managers were invited. Several meetings were also held to discuss the development of the new SBT Aquaculture Strategic Plan – Towards 2012, ensuring that research priorities reflected the needs of

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the SBT industry. Individual projects were then assessed to ensure that they met the priorities of the current strategic plan, as well as the objectives and outcomes published in the Aquafin CRC Commonwealth Agreement. As previously, new project pre-proposals were sourced through the existing FRAB processes. These were reviewed by the Scientific Advisory Committee, where necessary using the feasibility-attractiveness model used by the SAFRAB, and recommendations provided to the Steering Committee who undertook a similar assessment procedure in conjunction with the relevant five year Strategic R&D Plan. Applicants were advised of the outcome and the desirability to proceed with a full application, and relevant FRABs advised of the outcomes. Full applications were then received in October each year and the same process repeated. Subprogram recommendations as to the priority ranking of new projects were then provided to the relevant FRABs and FRDC in December of each year. Infrastructure The field infrastructure available in Port Lincoln to the SBT Aquaculture Subprogram was primarily provided by the SBT industry as for most of the period of this project research involving live tuna was undertaken on commercial farms, although some short-term and small scale research requiring calm conditions was undertaken on a lease held by SARDI in Rotten Bay, south of Boston Island. The details of each coming year's activities were incorporated in the Annual Operating Plan provided to FRDC in December of each year. Office space at SARDI Aquatic Sciences and the Lincoln Marine Science Centre were used by the Subprogram Leader and the Subprogram Project Officer, as well as a number of researchers working on SBT related Aquafin CRC-FRDC projects. These facilities were contributed by the South Australian Government through the South Australian Research and Development Institute (SARDI) and Flinders University of South Australia (FUSA). The extensive outdoor tank facilities at the SARDI South Australian Aquatic Sciences Centre (SAASC), West Beach, Adelaide were used for some nutrition research using surrogates. SARDI also provided access to its Australasian Experimental Stockfeed Extrusion Centre (AESEC) and PPPI analytical nutrition laboratory at the University of Adelaide's campus at Roseworthy. Operational Plans and Budgets The SBT Aquaculture Subprogram provided FRDC with an Annual Operating Plan at the end of each year, which confirmed/clarified outcomes and expenditure for the previous year, as well as a detailed forward operational plan. Each project within the Subprogram reported face-to-face to the Subprogram Steering Committee twice per year and completed milestone reports as specified in their approved application. Budget Reporting For each project the Principal Investigator's organisation continued to receive and be accountable for the income and expenditure associated with the funds received from the Aquafin CRC and the FRDC and offered in-kind in that project. Each Principal Investigator, following liaison with the Program Leader, reported expenditure to the

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Aquafin CRC and the FRDC as part of their six monthly progress report. The Subprogram Leader also collated, summarised and provided data for all projects to the Aquafin CRC and the FRDC as part of the six monthly progress reports for this project. Any income received from consultancy trials, royalties, publication and SBT sales, where these resources were owned by the Aquafin CRC and the FRDC, were credited to the relevant project budget line and reported in six monthly progress reports to the Aquafin CRC and the FRDC along with expenditure. Communication and Technology Transfer One of the objectives of this project was to optimise industry interaction to ensure that decisions were aligned with industry direction and to transfer research results rapidly and comprehensively to industry. Early in this project a number of strategic directions were identified to improve these activities including David Ellis, Research Manager for the SBT aquaculture industry, increasingly taking on the responsibility for the coordination between the Subprogram researchers and industry members. It was also proposed that the industry established its own R&D committee to discuss and provide a more cohesive input to the Subprogram Steering Committee. This has recently been achieved with the establishment of the ASBTIA Research Committee. The Subprogram ensured effective technology transfer through a variety of mechanisms which included: • Regular industry newsletters ‘Tuna-brief’, proving industry with regular updates on research outcomes. • Organising an annual industry workshop, including a workshop proceedings, proving a forum for industry and researchers to discuss the past years results and the future direction of research. • Organising six monthly science group meetings that focussed on research. • Organising quarterly Steering Committee meetings (two face to face and two teleconferences) to monitor the progress of each project within the Subprogram; discuss the priority of project proposals; to ensure that project were aligned to the Strategic R&D Plan; and to generally deal with any governance issues related to the Subprogram. • Facilitating other focussed industry workshops (e.g. SBT health workshop, product quality workshop, workshops on how to use software developed as part of the Aquafin CRC - SBT Aquaculture Subprogram, etc.). • Further developing and maintaining an SBT Aquaculture Subprogram website. This included a public areas and two password protected areas, one for the tuna industry and one for the Steering Committee.

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Strategic Planning A Strategic R&D Plan had been developed under the previous SBT Aquaculture Subprogram project – Southern Bluefin Tuna Aquaculture Strategic R&D Plan 20012006: Sustainability & Innovation for the Future. The Strategic R&D Plan was revisited annually, either through dedicated workshops or as part of annual conferences and ad-hoc workshops, to ensure that the plan was still relevant to the industry. This was extensively revised in consultation with stakeholders as part of the current project to produce a new five year plan – Southern Bluefin Tuna Aquaculture Strategic R&D Plan – Towards 2012: Striving for a Profitable and Sustainable Future.

RESULTS AND DISCUSSION Industry Development During the time of this project, 2004 to 2008, the SBT aquaculture industry has produced a relatively constant volume of product of between about 8000 to 9000 tonnes per annum, primarily dictated by the set Australian quota of 5265 tonnes/annum, most of which is used for farming. However, over this same period there has been a steady increase in the sale price of product from about 1400 to 2100 yen/kg (Figure 1, ASBTIA data), and this has been responsible for the partial recovery of the per annum value of the industry to its peak in 2000–01 (ABARE 2008).

Figure 1. Weight (tonnes) and value (yen/kg) of southern bluefin tuna produced in South Australia over the period 1993-2007 (figures sourced from the Australian Southern Bluefin Tuna Industry Association).

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Research Funding The ASBTIA (previously TBOASA) has and continues to voluntarily pay 0.25% of its gross value of production (GVP) to FRDC each year based on a MOU, which has ensured that the capacity existed to leverage funds to address industry priority R&D issues on a competitive national basis. ASBTIA committed a total of approximately $2.73M to FRDC over the years 2001-2008 (Aquafin CRC figures). Over this seven-year period the total cash expenditure on SBT projects managed by the SBT Aquaculture Subprogram was estimated to be $15.35M (Aquafin CRC figures). This expenditure (not including research organisation and industry in-kind) is contributed as follows: TBOASA/ASBTIA $2.73M FRDC (excl ASBTIA) $2.92M SA Government $3.30M CRC (incl Flinders, Gibsons) $6.39M The leverage on ASBTIA funds over this time is thus just over 5.6:1 in cash terms. Inclusion of in-kind contributions by research organisations approximately doubles this value to 11:1 Actual expenditures in the Aquafin CRC varied widely from year to year as payments are made on a milestone-completed basis and as projects began and finished. Research funds over this period were allocated across the six Aquafin CRC Program Areas (Propagation; Production; Value-adding; Health; Environment; and Subprogram Management) as shown in figure 2.

Subprogram Management, $714,957, 5%

Propagation, $48,469, 0% Production, $5,920,690, 38%

Environment, $3,974,231, 26%

Propagation Production Value-Adding Health Environment Subprogram Management

Health, $1,850,381, 12% Value-Adding, $2,969,825, 19%

Figure 2. Breakdown of Aquafin CRC Southern Bluefin Tuna Research funds (including contributions from Aquafin CRC, FRDC, ASBTIA, and the SA Government), into Aquafin CRC Program Areas (Propagation; Production; Value-adding; Health; Environment and Subprogram Management).

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Strategic Planning The previous Strategic Plan ‘Southern Bluefin Tuna Aquaculture Strategic R&D Plan 2001-2006: Sustainability & Innovation for the Future’ outlined nine focus areas ‘Feeds and Nutrition; Environment; Farm Husbandry and Management; Health; Product Diversification, Quality and Management; Propagation; Communication; People Development and Support; and Work Environment’. All of these nine focus areas were well addressed through Aquafin CRC and FRDC projects that were part of the previous (FRDC Project No. 2001/250) and current Subprogram projects. As part of the current Subprogram project a new Strategic R&D Plan was developed. The new strategic plan ‘Southern Bluefin Tuna Aquaculture Strategic R&D Plan – Towards 2012: Striving for a Profitable and Sustainable Future’ describes the local and international environment in which the SBT Aquaculture Subprogram operates to achieve the desired R&D outcomes and the strategies to achieve the desired outcomes. The SBT Aquaculture Strategic R&D Plan – Towards 2012 covers the strategic direction of the wild-caught SBT Aquaculture industry only and does not include the strategic directions of the hatchery-reared SBT Aquaculture industry sector, a decision of the Steering Committee. The ASBTIA have undertaken to amend this document in the future to include the strategic direction of the SBT wild fisheries sector. A summary of the present aims and strategies associated with SBT farming as per the SBT Aquaculture Strategic R&D Plan – Towards 2012, is listed below (Table 1).

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Table 1. Present aims and strategies associated with southern bluefin tuna farming (an extract from the Southern Bluefin Tuna Aquaculture (Wild-Capture) Strategic R&D Plan – Towards 2012: Striving for a Profitable and Sustainable Future; Clarke and Ham (Eds, 2008))

1. A Responsive Regulatory Environment & Abundant Resources Aim

Strategies

To ensure a ready supply of stock for farming.



Support the management of a sustainable SBT wild fishery through the provision of relevant farm based research.

To ensure availability of lease sites is not limiting for existing or future SBT farmers. To optimise baitfish availability and quality for use as feed. To plan for environmental change so as to optimise benefits and minimise risks. To ensure a ready source of capable employees.

• • •

Ensure adequate environmental knowledge exists to allow for development of appropriate policy Engage government regulatory agencies and the community in R&D outcomes. Through independent research maintain community and government confidence in industry practices.



Support the management of sustainable baitfish wild fisheries through the provision of relevant farm based research.



Support climate change R&D that characterises possible benefits, risks and management options.

• • •

Contribute to the development and review of education and training programs. Ensure that R&D outcomes are rapidly incorporated into curricula. Workshops (training).

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2. Cost-Efficient & Responsible Production Systems To grow the size and value of the industry.

• •

To improve the efficiency of industry farming operations so as to decrease operating costs.

• • • •



To minimise the possible environmental and disease risks associated with tuna farming through a proactive approach and enhanced knowledge.

• • •



Support longer term holding initiatives by participating in industry trials, collating the resultant information and improving feeding strategies. Improve overall growth consistency by better handling practices to reduce stress, and optimising feeding strategies for different age classes of SBT. Understand and better manage the effects of stress on SBT growth, condition and survival. Increase SBT production efficiency through targeted R&D to further refine baitfish feeds and feeding techniques. Understanding tuna physiology to target better nutrition to improve growth and feed efficiency Further improve manufactured pelleted feeds for use as supplementary or primary feeds by enhancing their acceptance by SBT and reducing costs through optimal ingredient selection (including fishmeal and oil replacement). Improve growth rates on manufactured pelleted feeds and reduce feed conversion ratios. Support engineering R&D to develop or introduce and refine existing farm husbandry infrastructure (e.g. fouling management).

Establish real time or early environmental warning systems, relevant environmental trigger points, and farm husbandry and management responses to address the key issues identified. Continuously refine SBT farming data and existing hydro-biogeochemical models of the tuna farming regions to proactively define, evaluate and manage risks. Support proactive research that identifies potential disease issues, develops rapid and cost effective diagnostic capabilities, and addresses the epidemiology of diseases thereby potentially enhancing solutions through animal husbandry and farm management practices (e.g. Uronema). Reduce SBT losses and stress related issues through optimised management of predators.

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3. An Optimised & Integrated Supply Chain To optimise product quality and safety so as to maximise financial returns and improve market competitiveness.

To develop a market & consumer culture.

• • • • •

Continue to align sampling methodologies and collect appropriate environmental residue data to proactively address changing trade standards for existing and new markets. Develop and implement a sophisticated and reliable product trace system to achieve world’s best practise in safety management and provide for markets increasingly desiring more knowledge of their product. Enhance mechanisms to control and optimise product quality characteristics Leverage R&D outcomes to promote the reputation of farmed SBT and its differentiation from competitors. Develop integrated bioeconomic farm support systems in the forms of models and software.



Through market R&D (consumers, wholesalers and retailers) identify and improve farmer and researcher knowledge of the key factors affecting product purchases and how these factors can be enhanced along the whole-of-supply chain.



Support the continuation of the SBT Aquaculture Subprogram in providing industry leadership in identifying, managing and communicating R&D outcomes, as well as forging strong relationships between industry, Government regulators and researchers.



Establish world leading R&D by supporting the development of infrastructure and organisational capability to attract people that can efficiently and effectively address SBT R&D needs. Manage R&D expenditure to develop and retain capable R&D resources. Establish linkages with preferred service providers who target and achieve industry priority R&D outcomes through an integrated multidisciplinary team approach. Share R&D information between industry participants to facilitate the development of decision support tools. Support education and training of industry personnel so that R&D is understood, supported, and rapidly and appropriately applied by industry.

4. People & Partnerships To empower industry to effectively participate in and influence key decision making. To strive for world’s best R&D that delivers a profitable, innovative and continuously improving industry.

• • • •

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To facilitate continuous improvement in OHS&W.



Support engineering R&D and operational solutions to improve OHS&W for employees of commercial SBT farming companies and research organisations, thereby reducing potential insurance liabilities.



Promote the leadership role that the industry provides in sustainable aquaculture development. Use transparency to minimise risk from NGO criticism. Enhance communication of R&D to highlight the direct and indirect benefits of the industry to Government and the community. Communicate the outcomes of R&D on the environment to promote industry’s continuous improvement program. Enhance local and international public awareness through the provision of targeted information on web sites, in newsletters and in communications with the media. Ensure Industry and R&D practices meet with or exceed relevant animal welfare regulatory standards.

5. Image & Communications To continuously improve the industry profile and image.

• • • •

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Projects The SBT Aquaculture Subprogram portfolio comprised the following Aquafin CRC projects: Appendix 3 summarises the Outputs and Outcomes of the research projects completed by May 2008. Production CRC 1A.9: FRDC 2004/212 - Aquafin CRC - FRDC - SBT Aquaculture Subprogram: assessment of alternative platforms for southern bluefin tuna research. Principal Investigator: Wayne Hutchinson (SARDI). COMPLETED. CRC 1A.11: FRDC 2004/205 - Aquafin CRC – Southern Bluefin Tuna Aquaculture Subprogram: longer term holding of SBT. Principal Investigator: David Ellis (ASBTIA). DRAFT FINAL REPORT NEAR COMPLETION. Nutrition & Feed Technology CRC 1A.4: FRDC 2001/249(revised) - Aquafin CRC - SBT Aquaculture Subprogram: Optimisation of farmed southern bluefin tuna (Thunnus maccoyii) nutrition to improve feed conversion efficiency and reduce production costs. Principal Investigator: Robert van Barneveld (Barneveld Nutrition). DRAFT FINAL REPORT NEAR COMPLETION. CRC 1A.7: FRDC 2003/228 - Aquafin CRC - SBT Aquaculture Subprogram: activity metabolism in live-held southern bluefin tuna (Thunnus maccoyii). Principal Investigator: Richard Musgrove (SARDI) (previously Russell Baudinette, University of Adelaide). COMPLETED. CRC 1A.7(2): FRDC 2005/200 - Aquafin CRC - SBT Activity metabolism in liveheld southern bluefin tuna (Thunnus maccoyii), Phase 2. Principal Investigator: Roger Seymour (University of Adelaide). COMPLETED. Product Quality CRC 1A.2(2): FRDC 2000/221.20 - Aquafin CRC - FRDC Southern Bluefin Tuna Aquaculture Subprogram: quality and nutritional evaluation of baitfish used for tuna farming project 2000/221 (extension). Principal Investigator: Jeff Buchanan (SARDI). COMPLETED. CRC 1A.10: FRDC 2004/211 – Aquafin CRC - FRDC - SBT Aquaculture Subprogram: nutritional profiles of baitfish 3: effects of harvest and post-harvest processes on quality of local pilchards for feeding SBT. Principal Investigator: John Carragher (SARDI). COMPLETED. CRC 2.2: FRDC 2001/248 - Aquafin CRC - SBT Aquaculture Subprogram: maximising the control of quality in farmed SBT. Principal Investigator: Philip Thomas (Flinders University). COMPLETED.

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CRC 2.2(2): FRDC 2004/209 - Aquafin CRC - SBT Aquaculture Subprogram: dietary supplements for reducing oxidative stress and improving flesh quality attributes in SBT. Principal Investigator: Philip Thomas (Flinders University). DRAFT FINAL REPORT SUBMITTED. CRC 2.2(3): FRDC 2004/209(extension) - Baitfish extension project. Principal Investigator: Trent D’Antignana (Flinders University). DRAFT FINAL REPORT SUBMITTED. Food Safety CRC 2.1: FRDC 2003/227 - Aquafin CRC - SBT Aquaculture Subprogram: development and validation of baitfish sampling methods to address international residue standards for southern bluefin tuna (Thunnus maccoyii). Principal Investigator: David Padula (SARDI). COMPLETED. CRC 2.1(2): FRDC 2004/206 - Aquafin CRC - SBT Aquaculture Subprogram: management of food safety hazards in farmed southern bluefin tuna to exploit market opportunities. Principal Investigator: David Padula (SARDI). COMPLETED. Environment CRC 4.3.1: FRDC 2001/102 - Aquafin CRC - SBT Aquaculture Subprogram: tuna environment subproject 1. Development of novel methodologies for cost effective assessment of the environmental impact of aquaculture. Principal Investigator: Maylene Loo (previously Anthony Cheshire) (SARDI). COMPLETED. CRC 4.3.2: FRDC 2001/103 - Aquafin CRC - SBT Aquaculture Subprogram: tuna environment subproject 2. Evaluation of waste composition and waste mitigation strategies. Principal Investigator: Milena Fernandes (previously Anthony Cheshire) (SARDI). COMPLETED. CRC 4.3.3: FRDC 2001/104 - Aquafin CRC - SBT Subprogram: tuna environment subproject 3. Development of regional environmental sustainability assessments for tuna sea-cage aquaculture. Principal Investigator: Jason Tanner (previously Anthony Cheshire) (SARDI). COMPLETED. CRC 4.5: FRDC 2003/226 - Aquafin CRC - Southern Bluefin Tuna Aquaculture Subprogram: pilot study into the use and efficacy of antifoulants on the nets used for southern bluefin tuna (Thunnus maccoyii) culture, including residue status of tuna and the surrounding environment. Principal Investigator: Kirsten Rough (ASBTIA). DRAFT FINAL REPORT SUBMITTED.

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CRC 4.5(1): FRDC 2003/226(extension) - extension to current Aquafin CRC/FRDC SBT Projects – 2003/226. Principal Investigator: David Ellis (ASBTIA). DRAFT FINAL REPORT SUBMITTED. CRC 4.6: FRDC 2005/059 - Aquafin CRC - SBT Aquaculture Subprogram: risk and response - understanding the tuna farming environment. Principal Investigator: Jason Tanner (SARDI). COMPLETED. SBT Health CRC 3.5: FRDC 2003/225 - Aquafin CRC - SBT Aquaculture Subprogram: investigation of the relationship between farming practices and southern bluefin tuna health. Principal Investigator: Barbara Nowak (University of Tasmania). COMPLETED. CRC 3.6: FRDC 2004/085 - Detection of SBT pathogens in environmental samples. Principal Investigator: Kathy Ophel-Keller (SARDI). COMPLETED. CRC 3.7: FRDC 2006/225 - Aquafin CRC - SBT Aquaculture Subprogram: improving husbandry and performance of southern bluefin tuna through better understanding of the relationship of fish stress and health. Principal Investigator: Barbara Nowak (University of Tasmania). ONGOING. Infrastructure & Management CRC 1A.5: FRDC 2002/249 - Aquafin CRC – Southern Bluefin Tuna Aquaculture Subprogram: service delivery and infrastructure management for projects requiring Port Lincoln based R&D support. Principal Investigator: Jeff Buchanan (SARDI). COMPLETED. CRC 1A.11: FRDC 2004/205 - Aquafin CRC - SBT Aquaculture Subprogram: provision of research platforms for projects requiring Port Lincoln based R&D support. Principal Investigator: David Ellis (ASBTIA). DRAFT FINAL REPORT SUBMITTED.

OUTPUTS The Subprogram outputs are highlighted on a year by year basis under the headings: Steering Committee Meetings, Science Group Meetings, Industry Workshop, Other Workshops and Other Events. 2004 Steering Committee Meetings Successful Subprogram Steering Committee meetings were held quarterly with detailed agenda and briefing papers distributed prior to meetings. Each year, two meetings were face-to-face in Port Lincoln and two by tele-conference. The format of 19

briefing papers was improved this year, including detailed contents pages and distribution of briefing papers via both e-mail and the SBT Aquaculture Subprogram web site. The SBT Aquaculture Subprogram web site now contains historical briefing papers and minutes back to 1997 when the Subprogram was initiated. Scientific Group Meetings Three successful scientific group meetings were held during 2004 in Port Lincoln with attendance at each by all relevant project Principal Investigators, technical representatives of about eight of the 12 commercial SBT farming companies and a number of Subprogram Steering Committee members. The first meeting focussed on final matters associated with the arrival of the new seasons research tuna and initiation of the Annual Operating Plan. The second and third meetings provided an opportunity to discuss current research projects, coordination between projects, overcome difficulties within projects and discussion of the development of new project proposals. Industry Workshop (25th October, 2004; Boston Bay Winery, Port Lincoln) The annual Subprogram Industry Workshop was successfully held on 25th October 2004 at a new location, Boston Bay Winery, which proved a better venue for the increasing number of people attending. The workshop was well attended with representatives from eight of the 12 commercial companies participating. A guest speaker, Dr Tim Ward, SARDI, was invited to present at this year’s workshop on a matter related to the SBT Industry that was of considerable interest to attendees, ‘Pelagic ecology of a northern boundary current system: effects of upwelling on the production and distribution of sardine, anchovy and SBT in the Great Australia Bight’. This year written articles were received from all presenters and collated in an improved handbook format that had a more professional appearance. The preworkshop handbook was distributed to all members and uploaded to the SBT Aquaculture Subprogram web site. A final version of the handbook was also produced after all articles had been submitted through the SARDI internal publication review process. Other Workshops Tagging Workshop (4th March, 2004; Lincoln Marine Science Centre, Port Lincoln) The SBT Aquaculture Subprogram (supported by FRDC and the Aquafin CRC) held a tagging workshop at the Lincoln Marine Science Centre on 4th March 2004. David Hall (Hallprint Pty Ltd), Kevin Williams (SBT Tagging Consultant) and John Gunn (CSIRO) were guest speakers. They presented up-to-date information based on their knowledge and experience with finfish tags, tagging and finfish behaviour during this process. The workshop included Microsoft PowerPoint presentations and practical demonstrations, specifically on dart tags and archival tags. The workshop was well attended by industry and scientists. SBT Health Workshop (13/14th May, 2004, Lincoln Marine Science Centre, Port Lincoln) The SBT Aquaculture Subprogram facilitated a two-day “SBT Health Workshop” held on May 13-14, 2004 at the Lincoln Marine Science Centre, Port Lincoln and organised by Dr Barbara Nowak and the SBT Health team as part of Aquafin CRC –

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SBT Aquaculture Subprogram project 2002/225: investigation of the relationship between farming practices and SBT health. The workshop leaders included Dr Colin Johnston and Dr Marty Deveney, PIRSA Fish Health Unit and Dr Barbara Nowak, University of Tasmania. The workshop concentrated on improving communication and transfer of information between fish farm workers and diagnostic pathologists and other fish health professionals. The workshop was attended by eleven participants, who all agreed that the workshop was well prepared and interesting. SBT Production Quality Workshop (27th May, 2004, Lincoln Marine Science Centre, Port Lincoln) The Subprogram facilitated a “Sensory Evaluation” workshop organised by Dr Philip Thomas, Flinders University, Principal Investigator of the project on SBT product quality, and his team. The workshop had previously been held at the Aquafin CRC conference in Adelaide in 2003. Due to a very positive response from SBT industry members that attended, a repeat of this workshop was held on 27th May 2004 in Port Lincoln. The interactive, hands-on workshop was well attended by Industry members with some 50 people in total. Tuna Quality Project Workshop (30th November, 2004, Boston Bay Wines, Port Lincoln) The Subprogram facilitated a Tuna Quality Project workshop, organised by Dr Philip Thomas and the SBT product quality team, held on the 30th November 2004 to present the outcomes of the tuna product quality research projects and to provide researchers and the SBT aquaculture industry with an opportunity to discuss their research and research directions. Written presentations were included in both the SBT Aquaculture Subprogram Industry Workshop Handbook (2004) and the Tuna Quality Project Workshop Handbook (2004). Representatives from seven of the twelve commercial SBT farming companies attended the workshop, as did a representative (Phil Clancy) from NIR Technology Australia, who had participated through a Memorandum of Understanding associated with research into the use of NIR for SBT and baitfish. Other Events The Subprogram Leader has attended several meetings with two tuna companies to finalise contract agreements associated with two AusIndustry Start Graduate Employee applications and participate in the management of the Graduate Employee research projects once they commenced. He also took part as a member of an Aquafin CRC working group to review the striped trumpeter project of Stephen Battaglene, TAFI, which is within the Aquafin CRC Production program; liased with Geoff Allan and Stewart Fielder, NSW Fisheries, to facilitate approval of their new temperate finfish Aquafin CRC project, which is within the Aquafin CRC Production Program; represented Anthony Cheshire (SARDI)/Peter Fairweather (Flinders University) at two Aquafin CRC Board meeting when they were not available; attended Aquafin CRC JMAC meetings in Hobart, Melbourne and Adelaide; attended as Aquafin CRC leader of the Production and Value Adding Programs, an Aquafin CRC Technology Transfer workshop in Hobart involving the salmon scientists and industry representatives; attended as Aquafin CRC Leader of the Production and Value Adding Programs, the annual Salmon Subprogram Conference in Hobart, Tasmania; gave a presentation to the SAFRAB (October) on the Subprogram

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activities and new projects under development, and attended a new CRC planning workshop in Melbourne in November. The Subprogram Project Officer was involved in the SARDI/Aquafin CRC/TAFI trade booth at the recent Australasia Aquaculture Conference in Sydney, September 2004, which provided an opportunity to promote the activities of the SBT Aquaculture Subprogram and the SBT Industry in general. 2005 Steering Committee Meetings Two face-to-face Steering Committee meetings in Port Lincoln (31st May and the 24th November) and teleconferences (14th February and the 25th August) were held. Briefing papers were prepared, the meeting conducted and minutes distributed to all members. Science Group Meetings Two face-to-face meetings were held (30th May and the 23rd November) in Port Lincoln. Recent progress on a range of Subprogram projects was presented at the May meeting with considerable exchange of information between scientists and also with technical people from the SBT farming companies. A number of new PhD students participated, in particular those associated with research on residues in SBT and baitfish. The workshop finished with presentations on and consideration of new project proposals. The November meeting addressed on-going research through a number of presentations. These talks were traditionally presented at the industry workshop, however due to the large number being presented this year the talks were split over the two days. Due to this, industry participation in the scientific group meeting was greater than at previous meetings. Extensive discussion occurred on the two new projects proposals ‘Aquafin CRC – SBT Aquaculture Subprogram: relationship between stress, SBT health and performance’, PI: Barbara Nowak, University of Tasmania and ‘Aquafin CRC – SBT Aquaculture Subprogram: longer term holding of southern bluefin tuna (Thunnus maccoyii), PI: David Ellis, TBOASA. Industry Workshop (22nd November, 2005, Boston Bay Wines, Port Lincoln) The Industry Workshop was held at the Boston Bay Winery again this year. The number of talks presented at the Industry Workshop had to be restricted to allow adequate discussion time, with additional talks being presented at the scientific meeting. Talks at the workshop were directed towards current industry issues, while talks presented at the scientific meeting were directed more towards on going research. The workshop was well attended by industry. Feedback from industry was that this was one of the most successful workshops to-date. The format of grouping talks into

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focus areas with discussion time at the end of each session worked well and encouraged industry to participate in discussions. A Draft Industry Handbook was prepared, distributed to all members and uploaded to the SBT Aquaculture Subprogram website. A Final Industry Handbook was produced and included some additional articles. The Handbook was edited through the SARDI internal publication review process prior to being distributed to all members and uploaded to the SBT Aquaculture Subprogram website by the end of February 2005. Other Workshops Fish Health Workshop (20th January, 2005; Lincoln Marine Science Centre, Port Lincoln) The SBT Aquaculture Subprogram facilitated a half-day training “SBT Health Workshop” organised by Dr Barbara Nowak, University of Tasmania. The workshop was attended by six industry members, two PhD students, one Aquafin CRC scientist and one SARDI scientist. Dr Mark Porter gave a presentation on stress in fish and Dr Barbara Nowak presented information on stress and health, including immune response. The workshop feedback was very positive. The workshop was interactive and included two competitions (list of factors which can induce stress in SBT and brief description of normal healthy SBT). Strategic R&D Planning Workshop (1 June, 2005; Hilton Motel, Port Lincoln) The SBT Aquaculture Subprogram ran a half-day workshop to discuss the development of the SBT Aquaculture Subprogram Strategic R&D Plan 2006-2011. The workshop was successful in highlighting the current industry drivers for SBT research and establishing the framework for the draft Strategic R&D Plan. The workshop was attended by most SBT research scientists, some Steering Committee members and a number of industry representatives. Aquafin CRC Technology Transfer Workshop (1 June, 2005; Hilton Motel, Port Lincoln) The SBT Aquaculture Subprogram facilitated a half-day “Technology Transfer Workshop”, organised by the Aquafin CRC. The workshop discussed and identified, from a researchers, regulators and industry point of view, different methods of transferring the results from Aquafin CRC - SBT Aquaculture Subprogram projects to end users, so that strategies for technology transfer and extension could be successfully developed within projects. Attendees at the workshop included representatives from some of the Steering Committee, most SBT research scientists, FRDC (2), Aquafin CRC (2) and the SBT Industry (4). Risk and Response Project Meeting (21st November, 2005, SARDI Aquatic Sciences, Adelaide) The Subprogram facilitated a Risk and Response project meeting, organised by Dr Jason Tanner, SARDI. The full-day meeting was held at SARDI Aquatic Sciences, involving key staff from SARDI, CSIRO and Adelaide University. The meeting allowed participants to gain a much better understanding of the overall project, and to put their components into perspective. It provided a venue for information exchange

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and decision making on what the key data requirements and tasks were for the next 6 12 months. New Health Proposal Workshop (16th January, 2006, SARDI Aquatic Sciences, Adelaide) The Subprogram facilitated a workshop on the new project proposal "Relationship between stress, SBT health and fish performance" (FRDC 2006/225) organised by Dr Barbara Nowak, University of Tasmania. The workshop took place at SARDI West Beach and was attended by Steve Clarke, John Carragher, Phil Thomas, Tim Clark, Marty Deveney, David Ellis, Daryl Evans, Emily Downes, Natalie Moltschaniwskyj, Mark Porter and Barbara Nowak. Workshop objectives were: • Considering the prior art on fish stress and performance, what measurements/observations do we want to make any other measurements than proposed? • Casting a broad net, what sensors, devices and methods might be available and likely to give useful data? • How do we access the technologies we want? A summary of the proposal was presented by Daryl Evans, which was then followed by discussion addressing the workshop objectives. 2006 Steering Committee Meetings In 2006 two face-to-face meetings in Port Lincoln (7th June and 30th November) and one teleconference (14th February) were held. Briefing papers were prepared, the meeting conducted and minutes distributed to all members. During the November meeting a new Chairperson was nominated (Richard Steven’s resigned as of this meeting due to other commitments and Rob Lewis, SARDI was suggested as his replacement); and FRDC funding proposals and Aquafin CRC Technology Transfer applications for the 2007/08 round were prioritised; in addition to the usual business of monitoring of research projects, milestone progress and budgets etc. Scientific Meetings Two face-to-face meetings were held at Boston Bay Wines, Port Lincoln (8th June and the 29th November). A record number of industry members attended the June meeting this year. This meeting focused on four main research areas: longer term holding of SBT; tuna health; metabolism; and environment. A brief update on the new Japanese residue standards and traceability requirements for farmed fish in Japan (from June 2006) was also given and a session held to demonstrate the content of the SBT Aquaculture

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Subprogram website to users and to give people an opportunity to change their password if desired. Most SBT aquaculture industry companies have representatives present. In November, as in the previous year, a number of additional talks that could not be presented at the Industry Workshop were presented at the scientific meeting. A miniworkshop was also held to coordinate the collation of the results of the longer term holding draft final project report. Industry representatives well attended the larger morning session of the scientific meeting. Industry Workshop (28th November, 2006, Boston Bay Wines, Port Lincoln) The number of talks presented at this year’s industry workshop was again restricted to allow adequate discussion time. Talks focused on the following research areas: Longer-term holding; Environment; Nutrition; Product Quality; and Health. The longer term holding presentations was one of the main components of the workshop, with this being the first time that these results have been presented to industry, and one of the first opportunities for researchers of the various project subcomponents to see each others results. A guest speaker, Dr John Middleton (SARDI), was also invited to present at the workshop to provide information on the oceanography research that is currently being undertaken at SARDI. He also provided an overview of the recently approved NCRIS Integrated Marine Observing System (IMOS), including the goals, instrumentation and specific plans for the South Australian region where some $5M in infrastructure will be deployed. He outlined the relevance of the proposal to the SBT industry and instigated discussion on the future location of key equipment and the potential relevance of climate change. Industry attendance was good, with a representative from Ridley Corporation also attending the meeting. Other Workshops Aquafin CRC technology transfer workshop (8th June, 2006, Boston Bay Wines, Port Lincoln) A second Technology Transfer Workshop was held as part of the SBT scientific meeting to identify suitable technology transfer and commercialisation activities that substantially improve the value to the end-users of the current Aquafin CRC’s project results and outputs. The process of submitting and prioritising proposals as outlined at the recent Steering Committee meeting was also discussed and all current proposals and their relative cost were listed. 2007 Steering Committee Meetings In 2007 two face-to-face meetings in Port Lincoln (20th June and 23rd November) and one teleconference (20th February) were held. The August teleconference was cancelled and urgent matters were dealt with out of session.

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Briefing papers were prepared, the meeting conducted and minutes distributed to all members. The February teleconference updated members on the status of current projects and other relevant issues (e.g. project proposals currently being considered by FRDC and the withdrawal of the TBOASA from the new Australian Seafood CRC). The status of the SBT Aquaculture Strategic R&D Plan and general communications was also provided. The June meeting focused on project proposals with respect to optimal utilisation of the remaining Aquafin CRC funds. The November meeting focussed on the final seven months of the Aquafin CRC: what needs to be done; what are the key dates; and who is responsible for what. Specifically the Steering Committee focussed on facilitating the development of an MOU between FRDC and the ASBTIA; considering what governance process will replace the current Subprogram at the end of the Aquafin CRC; developing a process for developing and approving projects for the 2008/09 funding cycle in the interim between the new governance process; completing the new Strategic R&D Plan; ensuring projects deliver the required outcomes and outputs; and determining what funds remain unspent and what additional project extensions should be considered. Scientific Meeting (19th June, 2007) One face to face scientific meeting was held at the Boston Bay Winery, Port Lincoln (19th June). The meeting was well attended by industry and presentations were informative and suitably targeted for an industry audience. A number of project outputs, including the Nutrition Manual (FRDC Project No. 2001/249; PI: Robert van Barneveld, Barneveld Nutrition), Health CD (FRDC Project No. 2003/225; PI: Barbara Nowak, University of Tasmania) and Product Quality poster (FRDC Project No. 2004/209; PI: Philip Thomas, Flinders University), were distributed to researchers and industry. Industry Workshop (21st November, 2007, Boston Bay Wines, Port Lincoln) This year’s Industry Workshop was once again held at the Boston Bay Winery. With the end of the Aquafin CRC less than 12 months away and the number of active projects reducing, presentations this year were focussed on Product Quality and Residues, Nutrition, Health, and Environment. Talks from active projects focussed on giving a final summary of outcomes to industry, and prioritising the research that would be done over the next seven months so as to ensure projects deliver their planned outcomes. Bob Gibson from Adelaide University was a guest speaker at the workshop giving a presentation on the human health benefits of eating seafood. His talk focussed on the nutrients in high lipid content fish such as tuna that can be beneficial to the health of humans as well as assist in preventing and treating heart disease. He outlined a number of current studies that are aiming at providing scientific evidence for the health benefits of eating seafood, drawing public attention towards this issue. He also 26

outlined competitors from other food sources in the market place, such as omega 3 enriched eggs and meats, that will be vying for the same share of the market place. The workshop was well attended by industry. Other Workshops Carbon Deposition Model Workshop (20th November, 2007, Lincoln Marine Science Centre, Port Lincoln) A workshop was held to demonstrate the carbon deposition model (Farmér) developed as part of the recently completed RESA project funded by the Aquafin CRC. This model allows farm managers to examine the spatial extent of likely benthic impacts underneath an individual cage or group of cages on a single lease. Understanding this footprint allows pontoons to be located within a lease in such a way as to reduce interactions between them and their effects on the benthos. This workshop was well attended by industry. Mortality Sampling Workshop (20th November, 2007, Sams Seafarm, Port Lincoln) A ‘hands-on’ mortality sampling workshop, organised by David Ellis, Dr Barbara Nowak and Dr Craig Hayward, was held to demonstrate the correct way to collect samples from moribund SBT. The workshop was aimed at people working in the SBT industry. Attendees at the workshop were provided with dissecting kits and, after a demonstration by Marty Deveney and Craig Hayward, they were provided with a SBT mort to practice collecting samples from. By training people in the correct way to collect samples the amount of information obtained from a moribund SBT increases enormously, assisting in providing the necessary information to help understand complex health issues like the ‘six week mortality syndrome’. The workshop was well attended by industry and the feedback from industry was that this sort of ‘hands-on’ informal workshop was an ideal atmosphere for transfering technology. 2008 Steering Committee Meetings A teleconference was held in February (20th February, 2008) focussing on finalising the remaining budget, making sure projects were on track to finish on time and the new governance structure that will replace the current SBT Aquaculture Subprogram. The final face-to-face meeting was held in Port Lincoln (29th May, 2008) focussing on finalising outstanding matters, including due dates for final reports, delivering project outputs and budget matters. It was agreed at the meeting that the current Subprogram would continue until the end of September 2008 to finalise any Aquafin CRC matters and liase with David Ellis to transfer the responsibility associated with new issues to the new ASBTIA – FRDC governance structure. A final teleconference was held on 24th September 2008 and focussed on reviewing remaining project activity and ensuring all outstanding Aquafin CRC and Subprogram matters were appropriately redirected; primarily to the ASBTIA and FRDC. This was 27

also an opportunity to thank industry and research participants for their contributions to individual projects and the overall success of the Subprogram and Aquafin CRC. Scientific Meeting A half-day final scientific meeting was held in Port Lincoln (29th May, 2008) to provide an update on active projects and project outputs (Risk and response project, health and stress project, carbon deposition model, the SBT mircoalgal database, and the economic analysis from the longer term holding project). A guest speaker, Sophie Bestly (CSIRO) also gave a presentation on movement patterns and feeding behaviour of juvenile SBT. Aquafin CRC SBT Wrap-up Workshop (28th May, 2008, Port Lincoln Hotel, Port Lincoln) A workshop was held to give a final account of the achievements of the SBT projects within the Aquafin CRC in an informal atmosphere and to provide an opportunity for industry to discuss, with researchers involved over the 7 year period, different aspects of the projects including the results, outcomes, outputs and future directions of research. Finally, it was an opportunity to thank industry and research participants for their contributions to individual projects and the overall success of the Aquafin CRC. The workshop was well attended by both industry and researchers. Key speakers presented excellent summaries on each research area, followed by panel discussions. Feedback from attendees was that it was a great location, good format and well organised. Education and Training The SBT Aquaculture Subprogram’s involvement with the Aquafin CRC and to a lesser extent AusIndustry provided opportunities for further developing the capabilities of exisiting researchers in the SBT industry and in associated research organisations. The Aquafin CRC also provided educational training to a wide range of university graduate students, 17 (including Aquafin CRC adopted PhD students) having been involved with PhDs focused on SBT projects (Table 2). It has been pleasing to see that a number of the PhD graduates, have since been employed in areas directly benefiting the industry (2), or in aquaculture, aquaculture research and education in Australia (5) or overseas (2). Eleven PhD graduates are still completing their theses, two of these with existing positions in the SBT aquaculture industry.

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Table 2. Aquafin CRC SBT PhD Student Holderships (extract from the Aquafin CRC 2007/08 Annual Report) Name and Year of Commencement

University of Enrolment

Alistair Douglas (2001)

Flinders University

Trent D’Antignana (2002)

Thesis Title

Proj No.

Supervisors

Completed?

First Graduate Destination

Maximising the control of quality of farmed SBT

2.2

Dr John Carragher Dr Philip Thomas

Australian and Japanese Industry Team

Thesis submitted

Managing Director, Seafood Services Japan (based in Tokyo)

Flinders University

Improving the flesh quality of SBT by examining their biochemistry and physiology

2.2

Dr John Carragher Dr Philip Thomas

Mr David Ellis (TBOASA)

Awarded

Postdoctoral Researcher, Marine Innovation SA

Peter Lauer (2002)

Flinders University

Benthic metabolism adjacent to SBT pontoons in South Australia

4.3.1

Prof Anthony Cheshire Prof Peter Fairweather Dr Jason Tanner

Mr Daryl Evans (Marnikol)

Awarded

Environmental Assessment Officer PIRSA Aquaculture

Hamish Aiken (2004)

University of Tasmania

Epidemiology of the blood fluke

3.5

Assoc Prof Barbara Nowak Dr Craig Hayward

Ms Rebecca Paterson (Ajka Pty Ltd)

Writing up.

Aquatic Animal Health Officer - PIRSA Aquaculture _

Sita Balshaw (2005)

Flinders University

Toxicological basis of mercury bioaccumulation in farmed SBT

2.1 (2)

Prof John Edwards Dr Kirstin Ross Mr Ben Daughtry

Mr David Warland (Sekol)

Writing up

_ -

Alexandra Korte (2005)

Flinders University

Dietary and cellular antioxidants and antioxidant enzymes and their role in improving product quality and shelf life in SBT

2.2 (2)

Dr Kathy Schuller Dr Philip Thomas

Dr Mark Crane (CSIRO)

Writing up

Women’s and Children’s Hospital, Adelaide

Samuel Phua (2005)

University of Adelaide

Global modelling methodologies for predicting dioxin and PCB levels in

2.1 (2)

Dr Ken Davey Mr Ben Daughtry

Mr David Warland (Sekol)

Thesis submitted

_

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Industry Mentor

farmed SBT Paul Bierman (2006)

University of Adelaide

Emlyn Jones (2006)

Flinders University

The suitability of remote sensing of phytoplankton and water quality to monitor interactions between aquaculture and the environment in Spencer Gulf. Sediment resuspension and nutrient regeneration in the tuna farming zone

4.6

Dr Megan Lewis Dr Jason Tanner

Not assigned

Writing up

_

4.6

Dr Jochen Kaempf Dr Milena Fernandes

Not assigned

Writing up

CSIRO Marine & Atmospheric Research, Hobart

Table 3. Adopted Aquafin CRC PhD Students (extract from the Aquafin CRC 2007/08 Annual Report) Name and Year of Commencement

University of Enrolment

Thesis Title

Project No.

Supervisors

Industry Mentor

Completed?

Paul Armstrong (2002)

University of Tasmania

Phytoplankton ecology - The key issue whether aquaculture operations stimulate phytoplankton biomass.

4.2

Dr Chris Bolch Dr Peter Thompson

Not assigned

Writing up

Monitoring Officer, Bureau of Meteorology

Dr Sapto Putro (2002)

Flinders University

Spatial and temporal structures and distribution of macrobenthic assemblages inhabiting coarse sediments at SBT farms

4.3.2

Dr Ib Svane Dr Jeremy Robertson

Not assigned

Awarded

University Lecturer, Indonesia

Janene Thompson (2003)

Flinders University

The interaction between enzymatic and non-enzymatic mechanisms protection against oxidative stress in farmed SBT

2.2

Dr Kathy Schuller Dr Philip Thomass

Rachel Lawrie (was Australian Fishing Enterprises)

Writing up

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Graduate Destinations

Student Learning Centre Flinders University, and family business

David Ellis (2004)

University of Tasmania

Thermodynamic responses of SBT to different feeding regimes.

1A.11

Dr John Purser Prof Chris Carter

Not required

Experimental work progressing

Already employed as Research Manager for the ASBTIA

Dr Quinn Fitzgibbon (2004)

The University of Adelaide

Activity metabolism in live held SBT

1A.7

Assoc Prof Roger Seymour Dr Richard Musgrove

Did not want a mentor

Awarded

Post Doctoral position at the Tasmanian Aquaculture and Fisheries Institute

Shelley Harrison (2004)

Flinders University

Interactions between silver gulls and tuna aquaculture in the Port Lincoln area

4.3.3

Dr Jeremy Robertson Dr John Carragher Dr Simon Goldsworthy

David Ellis (TBOASA)

Writing up

SA Department of Environment & Heritage, Port Lincoln Community Engagement Sport Officer; SA Department for Environment and Heritage;

David Padula (2004)

University of Tasmania

Through-chain investigation of residues of economic importance in Australian farmed SBT: Rationale for harmonised sampling approaches for product integrity programs

2.1(2)

A/Prof Barbara Nowak Dr Andrew Pointon

Not required

Experimental work progressing

Already employed as Food Safety Researcher- SARDI Glenside

Daryl Evans (2006)

University of Tasmania

Stress indicators in farmed southern bluefin tuna, the influence of husbandry practices and the implications for fish health and performance

3.7

A/Prof Barbara Nowak

Already in industry

Experimental work progressing

Already employed (Marnikol Fisheries)

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Publications (2004-2008) Scientific papers Aiken HM, Bott, NJ, Mladineo, I, Montero, FE, Nowak, BF and Hayward, CJ 2007, ‘Molecular evidence for cosmopolitan distribution of platyhelminth parasites of tunas (Thunnus spp.)’, Fish and Fisheries, vol. 8, pp. 167-180. Aiken, H, Hayward C, Nowak BF 2006, ‘An epizootic and its decline of a blood fluke, Cardicola forsteri in farmed southern bluefin tuna, Thunnus maccoyii’, Aquaculture, vol. 254, pp. 40-45. Aiken, H, Hayward, C, Crosbie, P, Watts, M and Nowak, B 2008, ‘Serological evidence of an immune response in farmed southern bluefin tuna against blood fluke infection: Development of an indirect enzyme-linked immunosorbent assay’, Fish and Shellfish Immunology, vol. 25, pp. 66-75. Balshaw, S, Edwards, J, Daughtry, B and Ross, K 2007, ‘Mercury in seafood: Mechanisms of accumulation and consequences for consumer health’, Rev. Environ. Health, 2007, Apr-Jun, vol. 22, no. 2, pp. 91-113. Balshaw, S, Edwards, J, Ross, K and Daughtry, B 2008, ‘Mercury residue distribution in the muscle tissue of farmed southern bluefin tuna is inversely related to the lipid content of tissues’, Food Chemistry, 2008, Dec, vol.111, Issue 3, pp. 616-621. Buchanan, J and Thomas, P 2008, ‘Improving the color shelf life of farmed southern bluefin tuna (Thunnus maccoyii) flesh with dietary supplements of vitamins E and C and selenium’, Journal of Aquatic Food Product Technology, vol. 17, pp. 285-302. Cheshire, A and Volkman, J 2004, ‘Australians net benefits of sustainable fish farming’, Nature, vol. 432, p. 671. Deveney, MR, Bayly, JT, Johnston, CT and Nowak, BF 2005, ‘A parasite survey of farmed southern bluefin tuna (Thunnus maccoyii Castelnau)’, Journal of Fish Diseases , vol. 28, pp. 279-284. Fernandes, M, Angove, M, Sedawie, T and Cheshire, A 2007, ‘Dissolved nutrient release from solid wastes of southern bluefin tuna (Thunnus maccoyii, Castelnau) aquaculture’, Aquaculture Research, vol. 38, No. 4, pp. 388-397. Fernandes, M, Lauer, P, Cheshire, A and Angove, M 2007, ‘Preliminary model of nitrogen loads from Southern Bluefin Tuna Aquaculture’, Marine Pollution Bulletin, vol. 54, issue 9, 1321-1332. Fernandes, MB, Cheshire, A and Doonan, A 2006, ‘Sediment geochemistry in lower Spencer Gulf, South Australia: implications for southern bluefin tuna farming’, Australian Journal of Earth Sciences, vol. 53, pp. 421-432. Fitzgibbon, Q, Seymour, RS, Ellis, D, Buchanan, J 2007, ‘The energetic consequence of specific dynamic action in southern bluefin tuna (Thunnus maccoyii)’, Journal of Experimental Biology, vol. 210, pp. 290-298. Fitzgibbon, QP, Baudinette, RV, Musgrove, RJ, Seymour, RS 2008. ‘Routine metabolic rate of southern bluefin tuna (Thunnus maccoyii)’, Comparitive Biochemistry and Physiology AMolecular & Integrative Physiology, vol. 150 (2), June, pp. 231-238. Glencross, B, Carter, C, Gunn. J, van Barneveld, R, Rough, K and Clarke, S 2002, ‘Southern Bluefin tuna, Thunnus maccoyii’, In ‘Nutrient Requirments and Feeding of Finfish for Aquaculture’, CAB International. pp. 159-171. Glencross, BD, Clarke, SM, Buchanan, JG, Carter, CG and van Barneveld, RJ 2002, ‘Temporal growth patterns of farmed juvenile southern bluefin tuna, Thunnus maccoyii (castelnau) fed moist pellets’, Journal World Aquaculture society, vol. 33, part 2, pp. 138-145. Hayward C, Aiken H and Nowak B 2007, ‘Metazoan parasites on gills of southern bluefin tuna (Thunnus maccoyii) do not rapidly proliterate after transfer to seacages’, Aquaculture, vol. 262, no. 1, pp. 10-16.

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Hayward CJ, Aiken H, Nowak, BF 2008, ‘An epizootic of Caligus chiastos (Copepoda, Caligidae) on Southern Bluefin Tuna (Thunnus maccoyii) farmed in Australia’, Diseases of Aquatic Organisms, vol. 79, pp. 57-63. Hayward CJ, Aiken H, Nowak, BF 2008, ‘Epizootics of metazoan gill parasites did not threaten Southern Bluefin Tuna (Thunnus maccoyii) in an extended farming trial over summer months’, Veterinary Parasitology, vol.154, pp. 122-128. Johnston, C, Deveney, M, Nowak, BF 2008, ‘Gross and histopathological characteristics of two lipomas and a neurofibrosarcoma detected in aquacultured southern bluefin tuna (Thunnus maccoyii) in South Australia’, Journal of Fish Diseases vol. 31, pp. 241-247. Nowak B 2007, ‘Parasitic diseases in marine cage culture – An example of experimental evolution of parasites?’, International Journal for Parasitology, vol. 37, issue 6, pp. 581-588. Nowak, B 2003, ‘Assessment of health risks to southern bluefin tuna under current culture conditions’, Bulletin of European Association of Fish Pathologists, vol. 24, pp. 45-51. Nowak, B, Mladineo, I, Aiken, H, Bott, N and Hayward, C 2006, ‘Results of health surveys of two species of farmed tuna: southern bluefin tuna (Thunnus maccoyii) in Australia and northern bluefin tuna (Thunnus thunnus) in the Mediterranean’, Bulletin of the European Association of Fish Pathologists, vol. 26, pp. 38-42. Phua, STG, Ashman, PJ, Daughtry, BJ 2008, ‘Levels of polychlorinated biphenyls (PCB) and polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/F) in fillets of farmed Southern Bluefin Tuna (Thunnus maccoyii)’, Chemosphere, 73, pp. 915-922. Phua, STG, Davey, KR, Daughtry, BJ 2007, ‘A new risk framework for predicting chemical residue(s) - Preliminary research for PCBs and PCDD/Fs in farmed Australian Southern Bluefin Tuna (Thunnus maccoyii)’, Chemical Engineering and Processing, vol. 46, issue 5, pp. 491-496. Rough, KM, Nowak, BF and Reuter, R 2005, ‘Haematology and leucocyte morphology of wild caught southern bluefin tuna’, Journal of Fish Biology, vol. 66, pp. 1649-1659. Schuller, K, Korte, A, Crane, M and Williams, A 2006, ‘Immortal tuna created’, Australasian Science, vol. June 2006, p. 9. Thomas, PM, Carter, CG, Carragher, JF and Glencross, BD 2003, ‘Temporal changes in the blood chemistry of farmed southern bluefin tuna Thunnus maccoyii (Castelnau) after feeding and repeated sampling disturbance’, Aquaculture Research. Vol. 34, pp. 265-267. Thompson, JL, Thomas, PM and Schuller KA 2006, ‘Purification and properties of a glutathione peroxidase from southern bluefin tuna (Thunnus maccoyii) liver’, Comparative Biochemistry and Physiology Part B, Biochemistry and Molecular Biology, vol. 144, no. 1, May 2006, pp. 86-93.

Conferences and Workshops: Presentations, Published Abstracts and Posters Aiken, H 2006, ‘Epidemiology of blood fluke infection, Cardicola forsteri, infection in SBT’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 59-61. Aiken, H, Bott, N, Mladineo, I, Montero, F, Nowak, B and Hayward, C 2007, ‘Cosmopolitan character of tuna blood fluke Cardicola forsteri’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007. Aiken, H, Deveney, M, Hayward, C, Crosbie, P, Watts, M and Nowak, B 2007, ‘Evidence of immune response in farmed southern bluefin tuna naturally infected with the blood fluke Cardicola forsteri’, European Association of Fish Pathology. Aiken, H, Hayward, C and Nowak, B 2006, ‘Diagnostic methods for blood fluke infection in southern bluefin tuna’, 5th International Symposium on Aquatic Animal Health, San Francisco, USA, 2 - 6 September. Aiken, H, Hayward, C and Nowak, B 2006, ‘Epidemiology of the blood fluke Cardicola forsteri in southern bluefin tuna and northern bluefin tuna’, International Symposium for Veterinary Epidemiology and Economics, Cairns. 6-11 August.

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Aiken, H, Hayward, C, Crosbie, P and Nowak, B 2006, ‘Different diagnostic methods and their efficacy for Cardicola forsteri infection in farmed southern bluefin tuna Thunnus maccoyii’, Australasian Aquaculture, Adelaide. Aiken, H, Hayward, C, Crosbie, P, Watts, M and Nowak, B 2007, ‘Evidence of a protective immune response in farmed southern bluefin tuna naturally infected with the blood fluke Cardicola forsteri’, Australian Society of Parasitology Annual Conference, 2007. Aiken, H, Hayward, C, Deveney, M and Nowak, B 2004, Parasite survey in culture southern bluefin tuna (poster presentation), Proceedings of the Australasian Aquaculture Conference, Sydney, September 2004. Aiken, H, Hayward, CJ, Nowak, B 2005, ‘Effect of culture time on infection by the blood fluke, Cardicola forsteri, in farmed southern bluefin tuna, Thunnus maccoyii’, Aquafin CRC conference, Hobart, July 2005. Aiken, H, Hayward, CJ, Nowak, B 2005, ‘Effect of culture time on infection by the blood fluke, Cardicola forsteri, in farmed southern bluefin tuna, Thunnus maccoyii’, European Association of Fish Pathologist conference, Copenhagen, September 2005. Aiken, HM, Hayward, CJ and Nowak, BF 2004, ‘Effect of culture time on infection by the blood fluke, Cardicola forsteri, in cultured southern bluefin tuna, Thunnus maccoyii’ Proceedings of the Biotechnologies for Quality Conference, Aquaculture Europe 2004, Barcelona, Spain, 20-23rd October 2004. Balshaw S, Edwards J, Daughtry, B and Ross, K 2007, ‘The effects of farming mercury accumulation in southern bluefin tuna (Thunnus maccoyii): implications for consumer health’, Proceedings of the 17th Annual General Meeting: European Division, The Society of Environmental Toxicology and Chemistry, Porto, Portugal, 20-24th May 2007. Balshaw, S 2005, ‘Mercury bioaccumulation in southern bluefin tuna (Thunnus maccoyii)’, poster presentation, 6th World Congress on Seafood Safety, Quality and Trade, Sydney, 12th - 14th September. Balshaw, S and Daughtry, B 2006, ‘Predictive model development for mercury in farmed southern bluefin tuna’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 65-68. Balshaw, S, Daughtry, B, Ross, K and Edwards, J 2005, ‘Mercury residues in farmed southern bluefin tuna (Thunnus maccoyii): Distribution and Concentration in Tissues During Grow-out in South Australia’, poster presentation, Proceedings of Balancing the Scales, 6th World Congress on Seafood Safety, Quality and Trade, Sydney, 14-16 September 2005. Balshaw, S, Ross, K, Daughtry, B and Edwards, J 2005, ‘Mercury residues in farmed southern bluefin tuna (Thunnus maccoyii) distribution and concentration in tissues during grow out’, AIEH Conference. Bayly, G 2002, ‘Tuna pelleted feed – a commercialisation trial’, Aquafest 2002, Hobart. Bierman, P 2006, ‘Water column properties and hydrodynamics seaward of Boston Island, lower Spencer Gulf’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005, SBT Aquaculture Subprogram, pp. 105 - 111. Bierman, P, Lewis, M, Ostendorf, B and Tanner, J 2006, ‘The potential for remote sensing to monitor interactions between aquaculture and the environment’, Proceedings of ARSPC 2006: Earth observation - From science to solutions, 13th Australasian Remote Sensing and Photogrammetry Conference, Canberra. Bierman, P, Lewis, M, Ostendorf, B and Tanner, J 2006, ’Remote sensing of phytoplankton and water quality for monitoring interactions between aquaculture and the environment’, Oral presentation, 13th Australian Remote Sensing and Phytogrammetry Conference (ARSPC), Canberra, November, 2006. Bierman, P, Lewis, M, Tanner, J and Ostendorf, B 2006, ‘Remote sensing of the Spencer Gulf environment’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 26-28.

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Bierman, P, Lewis, M, Tanner, J and Ostendorf, B 2007, ‘Remote sensing of sea surface temperature and phytoplankton in Spencer Gulf’, Australian Marine Science Association - SA Post Grad Workshop. Bierman, P, Lewis, M, Tanner, J and Ostendorf, B 2007, ‘Seasonal variability in chlorophyll and sea surface temperature throughout Spencer Gulf, South Australia’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007. Bierman, P, Lewis, M, Tanner, J and Ostendorf, B 2008, ‘Satellite Derived Phytoplankton Variability In Spencer Gulf, South Australia’, Australian Remote Sensing and Photogrammetry. Bierman, P, Lewis, M, Tanner, J and Ostendorf, B 2008, ‘Understanding phytoplankton variability throughout Spencer Gulf, South Australia, via satellite derived chlorophyll-a’, International Geoscience and Remote Sensing Symposium conference in Boston, July 2008. Bott, N and Ophel-Keller, K 2005, ‘Development of real-time PCR assays to detect pathogens of southern bluefin tuna, Thunnus maccoyii in environmental and net samples’, FRDC Aquatic Animal Health Sub-program meeting. Cairns, Australia, July 26-28, 2005. Bott, N and Ophel-Keller, K 2006, ‘Detection of SBT pathogens in environmental samples’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005. SBT Aquaculture Subprogram, pp. 128 - 130. Bott, N and Ophel-Keller, K 2006, ‘Environmental detection of parasites of southern bluefin tuna, Thunnus maccoyii using Quantitative Real-time PCR’, The Australian Society for Parasitology Inc. and ARC/NHMRC Research Network for Parasitology Annual Conference. Gold Coast, QLD, Australia, July 2-5, 2006. Bott, N, Loo, M, McKay, A and Ophel-Keller, K 2007, ‘Molecular taxonomy and its application’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007. Bott, NJ and Ophel-Keller, K 2005, ‘Development of real-time PCR assays to detect pathogens of southern bluefin tuna, Thunnus maccoyii in environmental and net samples’, 12th Conference of the European Association of Fish Pathologists. Copenhagen, Denmark. September 11-15, 2005. Buchanan, J 2003, ‘Pilchard with added vitamins for SBT; Do they get in?’, Southern Bluefin Tuna Industry Workshop, 3rd November, Port Lincoln. Buchanan, J 2007, ‘Further developments in air stable pellets’, DRAFT SBT Aquaculture Subprogram Industry Workshop Handbook 2007, SBT Aquaculture Subprogram Industry Workshop, 21st November 2007, Port Lincoln. SBT Aquaculture Subprogram, p. 21-22. Buchanan, J and Thomas, P 2003, ‘Preliminary report on the effect of coating pilchards with dietary anti-oxidants to improve the shelf life of southern bluefin tuna (Thunnus maccoyii)’, Southern Bluefin Tuna Industry Workshop, Port Lincoln. Buchanan, J and Thomas, P 2004 ‘Coating pilchards with dietary anti-oxidants to improve the shelf life of southern bluefin tuna Thunnus maccoyii’, poster presentation, Proceedings of the Australasian Aquaculture Conference, Sydney, September 2004. Buchanan, J and Thomas, P 2006, ‘Baitfish quality: changed in vitamins’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005. SBT Aquaculture Subprogram, pp. 45 - 49. Buchanan, J and van Barneveld, J 2004, ‘Preliminary report on the digestibility of extruded tuna diets’, Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Buchanan, J and van Barneveld, R 2004, ‘Flattened pellets to produce improved apparent intake in southern bluefin tuna: Interim report for SBT Nutrition Project’, Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Buchanan, J and van Barneveld, R 2004, ‘Nutrition: optimising manufactured feeds (pellets) for southern bluefin tuna (Thunnus maccoyii) metabolic physiology’, poster presentation, SARDI, MISA launch. Buchanan, J, Carragher J, Ellis, D, Rough, K, van Barneveld, R, Thomas, P, Micke, W, Musgrove, R, Manning, A and Zammit, B 2006, ‘Feeding southern bluefin tuna baitfish – integrated

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research gets the best productivity and product quality out of a complex feed resource’, CDROM, Program and abstract book of the Skretting Australiasian Aquaculture Conference 2006, Adelaide. Buchanan, JG and Thomas, PM 2004, ‘Coating pilchards with dietary antioxidants to improve the shelf life of southern bluefin tuna (Thunnus maccoyii)’, proceedings of the Biotechnologies for Quality Conference, Aquaculture Europe 2004 Barcelona, Spain, 20-23rd October 2004. Carragher, J 2003, ‘Digital image analysis of colour and the quality of farmed SBT’, Southern Bluefin Tuna Industry Workshop. 3rd November, Port Lincoln. Carragher, J 2006, ‘Nutritional quality of local baitfish for feeding southern bluefin tuna’, SBT Aquaculture Subprogram Industry Workshop, June. Carragher, J, Ellis, D, Rough, K, Buchanan, J, Micke, W, Musgrove, R, Thomas, P and Manning, A 2006, ‘Effect of harvest and post-harvest processes on quality of local baitfish for feeding southern bluefin tuna’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005, SBT Aquaculture Subprogram, pp. 50 - 58. Carragher, JF 2005, ‘Nutritional quality of local baitfish for feeding southern bluefin tuna’, SBT Aquaculture Subprogram Industry Workshop, November. Cheshire, A 2003, ‘Preliminary investigations of the effects of a Wattyl anti-foulant on tuna farm nets’, Southern Bluefin Tuna Industry Workshop. 3rd November, Port Lincoln. Cheshire, A 2007, ‘Environmental management of finfish aquaculture in Australia: Lessons from the farming of southern bluefin tuna’, New Zealand Seafood Industry Conference, New Zealand. Cheshire, A 2007, ‘Environmental management of finfish aquaculture in Australia: Lessons from the farming of southern bluefin tuna’, New Zealand Aquaculture Industry Conference. Cheshire, A, Fernandes, MB, Loo, M and Lauer, P 2005, ‘The impact of tuna farming on benthic systems: effects and recovery’, World Aquaculture Society Annual International Meeting. Nusa Dua, Bali, Indonesia, May 9-13, 2005. Cheshire, A, Fernandes, MB, Svane, I, Tanner, J, Clarke, S and Branford, A 2002, ‘Evaluation of waste composition and waste mitigation strategies for tuna aquaculture’, Aquafin CRC-FRDC Industry Workshop, Book of Abstracts, Port Lincoln, Australia, 4 November, pgs 64-78. Cheshire, A, Loo, M, Ophel-Keller, K and McKay, A 2002, ‘Development of novel methodologies for cost effective assessment of the environmental impact of aquaculture’, Aquafin CRC-FRDC Industry Workshop, Book of Abstracts, Port Lincoln, Australia, 4 November, pgs 53-63. Cheshire, AC 2002, ‘Regional environmental monitoring of aquaculture: a risk assessment approach’, invited seminar, 13th Meeting of the Governing Council of NACA, Langkawi, Malaysia. January. Cheshire, AC 2002, ‘Regional environmental monitoring of aquaculture: A risk assessment approach’, invited seminar, Chinese Institute for Oceanology, Qingdao, China, August. Cheshire, AC 2002, ‘The effects of tuna pontoons on benthic systems: Modelling carbon and nitrogen outputs from a tuna pontoon’, Aquafin CRC Conference, Hobart, 22nd - 24th September. Cheshire, AC 2002, ‘Towards the development of a comprehensive environmental monitoring system for the Aquaculture Industry’, invited seminar, Nanyang University, Singapore, January. Cheshire, AC 2002, ‘Towards the development of regional environmental monitoring systems to ensure sustainable development of the aquaculture industry’, invited seminar, Symposium on Sustainable Fisheries and Aquaculture. 50th Anniversary Celebration of the Dalian Fisheries University, Dalian, China, September. Clarke S 2005, ‘Production program highlights’, Proceedings of the 3rd Aquafin CRC conference, Aquafin CRC, Hobart, p. 3. Clarke S 2005, ‘SBT Aquaculture Subprogram Report: 2005’, SBT Aquaculture Subprogram Industry Workshop, Draft Industry Handbook 2005, p.5-16. Port Lincoln, South Australia, 22nd November 2005.

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Clarke S 2006, ‘SBT Aquaculture Subprogram report: 2005’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005, SBT Aquaculture Subprogram, pp. 8 - 18. Clarke S and Ellis D 2006, ‘An overview of the success of the Aquafin CRC southern bluefin tuna research and development’, CD-ROM, program and abstract book of the Skretting Australasian Aquaculture Conference 2006, Adelaide. Clarke S and Ham J 2006, ‘Southern Bluefin Tuna Aquaculture Subprogram report: 2006’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 4-8. Clarke S and Ham J 2007, ‘Subprogram leaders report. DRAFT SBT Aquaculture Subprogram Industry Workshop Handbook 2007’, SBT Aquaculture Subprogram Industry Workshop, 21st November 2007, Port Lincoln. SBT Aquaculture Subprogram, pp. 3-8. Clarke S and Ham J 2007, ‘Draft SBT Aquaculture Subprogram Industry Workshop Handbook 2007, SBT Aquaculture Subprogram, p. 49. Clarke, S 2002, ‘Progress and future challenges for tuna R&D’, Aquafest 2002, Hobart. Clarke, S 2004, ‘SBT Aquaculture Subprogram Report: 2004’, Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Clarke, SM 2002, ‘Grow-out of southern bluefin tuna (Thunnus maccoyii) - the Australian Experience’, 1st International Symposium on Domestication of Bluefin Tuna, Cartagena, Spain; 3-8 February 2002. Clarke, SM 2003, ‘Aquaculture in South Australia’, informal presentation, PIRSA Animal Health Conference, Port Lincoln, February. Clarke, SM 2003, ‘SBT Aquaculture Subprogram Report’, Southern Bluefin Tuna Industry Workshop. 3rd November, Port Lincoln. Crane, M 2007, ‘Aquatic animal health in Australia - How disease free are we?’, 4th Aquafin CRC Conference, Barossa Valley, SA, 14-17 May. Crane, M and Williams, L 2004, ‘Aquafin CRC - FRDC Southern Bluefin Tuna Aquaculture Subprogram: tuna cell line development and their application to tuna aquaculture health surveillance’, Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. D’Antignana, T 2003, ‘Carcass mapping in commercially produced SBT fed a diet fortified with vitamins’, Southern Bluefin Tuna Industry Workshop. 3rd November, Port Lincoln. D’Antignana, T, Carragher, J and Thomas, P 2006, ‘The effect of dietary antioxidants and freezing on the flesh quality attributes of farmed southern bluefin tuna (Thunnus maccoyii)’, Australasia Aquaculture, Adelaide, August. D’Antignana, T, Carragher, J and Thomas, P 2006, ‘The effect of different combinations of dietary vitamins on the distribution of antioxidants in farmed southern bluefin tuna’, Australasian Aquaculture Conference, Adelaide, August. D’Antignana, T, Carragher, J, Buchanan, J and Thomas, P 2006, ‘The effect of dietary antioxidants and freezing on the flesh attributes of farmed southern bluefin tuna (Thunnus maccoyii)’, CDROM, program and abstract book of the Skretting Australiasian Aquaculture Conference 2006, Adelaide. D’Antignana, T, Carragher, J. and Thomas, P 2006, ‘The effect of different combinations of dietary vitamins on the distribution of antioxidants in farmed southern bluefin tuna’, CD-ROM, program and abstract book from the Australasian Aquaculture Conference 2006, August, Adelaide. D’Antignana, T, Thomas, M and Thomas, P 2007, ‘Baitfish nutrient injection: a practical approach to delivering dietary supplements to southern bluefin tuna’, DRAFT SBT Aquaculture Subprogram Industry Workshop Handbook 2007, SBT Aquaculture Subprogram Industry Workshop, 21st November 2007, Port Lincoln. SBT Aquaculture Subprogram, pp. 18-20.

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D’Antignana, T, Thomas, P and Carragher, J 2005, ‘Fresh vs Frozen Tuna – How do they compare?’, SBT Aquaculture Subprogram Industry Workshop, Draft Industry Handbook 2005, Port Lincoln, South Australia, 22nd November 2005, pp.123-124. D’Antignana, T, Thomas, P and Carragher, J 2006, ‘Fresh vs frozen tuna – how do they compare?’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005. SBT Aquaculture Subprogram, pp. 132 – 133. D’Antignana, T, Thomas, PM and Carragher, J 2004, ‘The effect of different dietary combinations of vitamin e, vitamin c and selenium on the distribution of these anti-oxidants in the carcasses of southern bluefin tuna (Thunnus macoyii)’, Aquaculture Europe, Biotechnologies for Quality, Barcelona, Spain. October 20-23. D'Antignana, T, Thomas, DM, Carragher, JF and Schuller, K 2002, ‘Biochemical and physiological factors affecting the composition and flesh quality characteristics of farmed southern bluefin tuna’, Aquafin CRC – southern bluefin tuna Aquaculture Subprogram, southern bluefin tuna Subprogram Industry Workshop, Port Lincoln, November. D'Antignana, T, Thomas, P and Carragher, J 2004, ‘The effect of different dietary combinations of vitamin E, vitamin C and selenium on the distribution of these anti-oxidants in the carcasses of farmed southern bluefin tuna (Thunnus maccoyii)’, Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. D'Antignana, T, Thomas, PM and Carragher, J 2004, ‘The effect of dietary combinations of vitamin E, vitamin C and selenium on the distribution of these anti-oxidants in carcasses of southern bluefin tuna (Thunnus maccoyii)’, Aquaculture Europe 2004 - Biotechnologies for Quality Conference Proceedings, Barcelona, Spain, 20-23rd October 2004. Daughtry, B, Phua, S, Balshaw, S, Madigan, T and Padula, D 2006, ‘Preliminary results for metal residue concentrations in farmed SBT during grow-out’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005, SBT Aquaculture Subprogram, pp. 59 - 64. Daughtry, BJ, Phua, S, Balshaw, S and Padula, DJ 2005, ‘Framework for modelling residue levels in farmed southern bluefin tuna’, 3rd Aquafin CRC Conference, Hobart, 5th - 7th July. Daughtry, BJ, Phua, STG, Padula, DJ 2005, ‘Evaluation of different approaches to calculate lower bound dioxin and PCB TEQs: blank detection issues’, AgriQuality 20th Conference of Residue Chemists, Wellington, New Zealand, 5-7 October 2005. Davey, K 2006, ‘Predictive modelling of newtonian cooling of freshly harvested fish in ice slurry’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 73-75. Deveney, M, Bayly, T and Nowak, B 2003, ‘Parasite survey in southern bluefin tuna in 2003 – Preliminary results’, Aquatic Animal Health Subprogram Scientific Conference, Geelong, Victoria. Deveney, M, Bayly, T and Nowak, B 2003, ‘Parasite survey in southern bluefin tuna in 2003 – Preliminary results’, Southern Bluefin Tuna Subprogram Industry Workshop, Port Lincoln. Deveney, M, Bayly, T and Nowak, B 2003, ‘Sampling and identification of SBT parasites’, Lincoln Marine Sciences Centre, Port Lincoln, 3rd November. Deveney, M, Bayly, T, and Nowak, BF 2003, ‘A parasite survey of southern bluefin tuna in 2003 preliminary results’, Aquafin CRC Conference, 28-30 October Adelaide, South Australia. Deveney, M, Hayward, C, Bott, N and Nowak, B 2007, ‘Uronema nigricans infections in farmed southern bluefin tuna (Thunnus maccoyii Castelnau)’, The 7th International Symposium of Fish Parasitology, Viterbo, Italy, 2007. Douglas, AE, Carragher, JF, Thomas, PM and Paterson, B 2002, ‘Do quality characteristics of farmed Southern bluefin tuna (Thunnus maccoyii) influence auction price in Japan?’, World Aquaculture Society (WAS) Meeting, Beijing, China. 23-27 April. Douglas, AE, Thomas, P, Carragher, JC and Bremner, A 2003, ‘Digital image analysis of colour and the quality of farmed southern bluefin tuna’, Aquafin CRC Conference, 28-30 October Adelaide, South Australia.

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Douglas, AE, Thomas, PM, Carragher, JF and Bremner, HA 2004, ‘Post-harvest management of farmed, fresh southern bluefin tuna from Port Lincoln to Japan: same day vs next day processing’, Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Elliott, N, Jackson, C, Van Barneveld, R, Gunn, J, Musgrove, R, Fitzgibbon, Q, Crane, M, Butler, R, Cook, M and Ophel-Keller, K 2003, ‘Innovative methodologies’, Aquafin CRC Conference, 28-30 October Adelaide, South Australia. Ellis, D 2006, ‘Innovation and current farming practices – southern bluefin tuna (Thunnus maccoyii) farming industry’, viewed .Proceed ings for the Aqua 2006 Conference, European Aquaculture Society and World Aquaculture Society, Florence, Italy, May. Ellis, D and Clarke, S 2006, ‘Southern bluefin tuna industry – current status and adaptive management strategies for sustainability’, CD-ROM, program and abstract book of the Skretting Australiasian Aquaculture Conference 2006, Adelaide. Ellis, D and van Barneveld, R 2005, ‘Response of farmed southern bluefin tuna (Thunnus maccoyii) to strategic feeding regimes using formulated combinations of baitfish’, SBT Aquaculture Subprogram Industry Workshop, Draft Industry Handbook 2005, p.17-25. Port Lincoln, South Australia, 22nd November 2005. Ellis, D and van Barneveld, R 2006, ‘Response of farmed southern bluefin tuna (Thunnus maccoyii) to strategic feeding regimes using formulated combinations of baitfish’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005. SBT Aquaculture Subprogram, pp. 19 - 27. Evans, D 2007, ‘Historical farm data’, DRAFT SBT Aquaculture Subprogram Industry Workshop Handbook 2007, SBT Aquaculture Subprogram Industry Workshop, 21st November 2007, Port Lincoln. SBT Aquaculture Subprogram, pp. 23-30. Fernandes, M 2003, ‘Waste characterisation and mitigation: natural and aquaculture loadings and the effects of fallowing. Southern Bluefin Tuna Industry Workshop’, 3rd November, Port Lincoln. Fernandes, M, Doonan, A and Cheshire, A 2004, ‘Revisiting the fallowing dataset: grain size and compositional trends of sediments’, Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Fernandes, M, Lauer, P and Cheshire, A 2004, ‘Nitrogen flows associated with tuna farming in Port Lincoln, South Australia’, Australian Marine Science Association Conference Proceedings, Hobart, Tasmania, 6-9 July. Fernandes, MB, Doonan, A and Cheshire, A 2004, ‘Revisiting the fallowing dataset: Grain size and compositional trends of sediments’, Aquafin CRC-FRDC Industry Workshop Handbook, Port Lincoln, Australia, 25th October, pp. 87-103. Fernandes, MB, Lauer, P and Cheshir,e A 2005, ‘Natural regeneration of the benthic environment: tuna aquaculture as a case study’, Australian Marine Sciences Association 2005 Meeting, Book of Abstracts, Darwin, Australia, July 11-13, 2005, p.73. Fernandes, MB, Lauer, P and Cheshire, A 2005, ‘Seasonal changes in SBT farm environmental inputs and their effect on sediments’, Aquafin CRC Conference, Conference Proceedings, Hobart, Australia, July 5-7, 2005, p.22. Fernandes, MB, Loo, M, Putro, S and Cheshire, A 2003, ‘Chemical and biological composition of sediments during fallowing’, Aquafin CRC-FRDC Industry Workshop, Book of Abstracts, Port Lincoln, 3rd November, pp. 71-76. Fernandes, MB, Loo, M, Svane, I and Cheshire, A 2002, ‘Experimental design for assessing the recovery of sediments under tuna pontoons during fallowing’, Aquafin CRC Conference, Hobart, Tasmania, 22-24 September. Fitzgibbon, Q, Clark, T, Seymour, R, Taylor, B, Frappell, P, Ellis, D, Carragher, J and Buchanan, J 2006, ‘Cardio-metabolic physiology of southern bluefin tuna: aiming to model expenditure’,

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Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005. SBT Aquaculture Subprogram, pp. 37 - 44. Fitzgibbon, Q, Ellis, D and Seymour, R 2007, ‘Relationship between metabolic rate and visceral warming in southern bluefin tuna (Thunnus maccoyii)’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007. Fitzgibbon, Q, Musgrove, R, Carragher, J and Buchanan, J 2004, ‘Activity metabolism in live-held southern bluefin tuna (Thunnus maccoyii)’, Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Fitzgibbon, Q, Musgrove, R, Seymour, R and Carragher, J 2004, ‘Metabolism: using a sea-cage based mesocosm to measure southern bluefin tuna (Thunnus maccoyii) metabolic physiology’, poster presentation, SARDI, MISA launch. Fitzgibbon, Q, Seymour, R, Ellis, D and Buchanan, J 2005, ‘The energetic consequences of specific dynamic action in southern bluefin tuna (Thunnus maccoyii)’, 3rd Aquafin CRC Conference, Hobart, 5th - 7th July. Fitzgibbon, Q, Seymour, R, Ellis, D and Buchanan, J 2006, ‘The energetic consequence of specific dynamic action in southern bluefin tuna (Thunnus maccoyii)’, program and abstract book of the Australian and New Zealand Society for Comparative Physiology and Biochemistry Conference 2005, Dunedin. Fitzgibbon, Q, Seymour, R, Ellis, D and Buchanan, J 2006, ‘The energetic consequence of specific dynamic action in southern bluefin tuna (Thunnus maccoyii)’, CD-ROM, program and abstract book of the Skretting Australiasian Aquaculture Conference 2006, Adelaide. Gibson, B 2007, ‘Health benefits of eating seafood’, DRAFT SBT Aquaculture Subprogram Industry Workshop Handbook 2007, SBT Aquaculture Subprogram Industry Workshop, 21st November 2007, Port Lincoln. SBT Aquaculture Subprogram, pp. 13-15. Goldsworthy, S, Hamer, D and Shaughnessy, P 2006, ‘Addressing seal interactions in the Finfish Aquaculture Industry’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005. SBT Aquaculture Subprogram, pp. 97 - 104. Harris, J, Leef, M and Powell M 2003, ‘Metabolic rate comparisons between Atlantic salmon, Salmo salar, infected with Neoparamoeba pemaquidensis or Tenacibaculum maritimum’, 20th Annual Australia and New Zealand Society of Comparative Physiology and Biochemistry Conference, Hobart, 5th – 7th December. Harrison, S, Carragher, J, Robertson, J, Svane, I, Ellis, D and Shimmin, G 2007, ‘Feathers and fins Seabirds at tuna farms: Problems, consequences and solutions’, European Aquaculture Society Conference, Turkey, October 2007. Harrison, S, Robertson, J and Carragher, J 2004, ‘Interactions between seabirds and tuna farms near Port Lincoln’, The Australiasian Management Wildlife Society, 17th Scientific Meeting Proceedings, November 2004, pg. 48. Harrison, S, Robertson, J, Carragher, J and Svane, I 2006, ‘Silver gulls in the Port Lincoln area’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005. SBT Aquaculture Subprogram, pp. 82 - 91. Harrison, S, Shimmin, G, Robertson, J, Carragher, J, Svane, I and Ellis, D 2006, ‘Egg oiling as a control method for an expanding Silver Gull population’, Proceedings of the 2006 Australian Wildlife Management Society conference, Auckland, New Zealand, 4-7 December 2006. Harrison, S, Shimmin, G, Robertson, J, Carragher, J, Svane, I and Ellis, D 2006, ‘Silver gulls and tuna farms: management and data calibration’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 76-79. Harrison, S, Robertson, J, Carragher, J, Svane, I and Cheshire, A. (2003). The interactions between seabirds and tuna farms near Port Lincoln. Proceedings of the 2nd Aquafin CRC Conference, 28-30 October Adelaide, South Australia. Hayward, C 2004, ‘Polyopisthocotyleans and their impact in marine fish culture’, As part of the PhD course, ‘Diagnosis and Control of Fish Diseases’. Royal Veterinary and Agricultural University, Copenhagen, Denmark, November.

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Hayward, C 2004, ‘Preliminary results from southern bluefin tuna health survey in 2004’, December 24 2004, Kinki University aquaculture field station, Uragami, Wakayama Prefecture, Japan. Hayward, C 2005, ‘Tuna health’, School of Aquaculture, University of Tasmania, September. Hayward, C Aiken, H Nowak, B 2005, ‘Metazoan parasites on the gills of southern bluefin tuna (Thunnus maccoyii) do not rapidly proliferate after transfer to culture cages’, FRDC Aquatic Animal Health Subprogram Scientific Conference, Cairns, July 2005. Hayward, C, Aiken, C and Nowak, B 2004, ‘Metazoan parasites on the gills of southern bluefin tuna (Thunnus maccoyii) do not rapidly proliferate after transfer to culture cages’, Australian Society for Parasitology Conference Proceedings, Western Australia, September 2004. Hayward, C, Aiken, H and Nowak, B 2006, ‘An epizootic of Caligus sp. (Copepoda, Caligidae) on southern bluefin tuna farmed in Australia’, CD-ROM, Proceedings of the International Symposium for Veterinary Epidemiology and Economics, Cairns, 6-11 September. Hayward, C, Aiken, H and Nowak, B 2007, ‘Epizootics of metazoan gill parasites did not threaten feasibility of farming southern bluefin tuna (Thunnus maccoyii) in a trial extending over summer months’, 7th International Symposium of Fish Parasites, Viterbo, Italy, 24-28 September 2007. Hayward, C, Aiken, H, I’Anson, C, Crosbie, P and Nowak, B 2006, ‘Effects of holding time on health of SBT’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005. SBT Aquaculture Subprogram, pp. 112 - 125. Hayward, C, Aiken, H, I'Anson, C, Crosbie, P and Nowak, B 2005, ‘Effects of holding time on health of SBT’, SBT Aquaculture Subprogram Industry Workshop, Draft Industry Handbook 2005, p.103-116. Port Lincoln, South Australia, 22nd November 2005. Hayward, C, Aiken, H, Nowak, B 2005, ‘Metazoan parasites on the gills of southern bluefin tuna (Thunnus maccoyii) do not rapidly proliferate after transfer to culture cages’, European Association of Fish Pathologist conference, Copenhagen, September 2005. Hayward, C, Bott, N and Nowak, B 2007, ‘Sea lice (Caligus spp.) infection in southern bluefin tuna’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007. Hayward, C, Bott, N, Aiken, H and Nowak, B 2007, ‘Sea lice (Caligus spp.) infections in southern bluefin tuna’, FRDC Animal Health Conference, Cairns, 2007. Hayward, C, Bott, N, Itoh, N, Iwashita, M, Okihiro, M and Nowak, B 2007, ‘Three species of parasites emerging on the gills of mulloway, Argyrosomus japonicus (Temminck and Schlegel, 1843) cultured in Australia’, poster presentation, 4th Aquafin CRC Conference, Barossa Valley, SA. 14-17 May. Hayward, C, Deveney, M, Aiken, H, Nowak, B 2005, ‘Monogenean infections of cultured southern bluefin tuna, Thunnus maccoyii, in 2003 and 2004’, Fifth International Conference on Monogenea, Guangzhou, China, August 2005. Hayward, CJ, Bott, NJ, Aiken, H and Nowak, B 2006, ‘An epizootic of Caligus sp. on farmed southern bluefin tuna (Thunnus maccoyii) off South Australia’, Proceedings of the 5th International Symposium for Aquatic Animal Health, San Francisco, 2-6 September 2006. Herzfeld, M 2006, ‘Pilot models of the Spencer Gulf and Boston Bay area’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005, SBT Aquaculture Subprogram, p. 94. Herzfeld, M, Andrewartha, J and Middleton, J 2007, ‘Hydrodynamic models used in Aquafin CRC numerical studies’, 4th Aquafin CRC Conference, Barossa Valley, SA. May 14-17. Hutchinson, W 2007, ‘Current status and issues confronting hatchery production of marine finfish in South Australia’, 4th Aquafin CRC Conference, Barossa Valley, SA. 14-17 May. Hutchinson, W and Buchanan, J 2004, ‘Aquafin CRC-SBT Aquaculture Subprogram Project 2004/212 "Assessment of alternative platforms for southern bluefin tuna research’, Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Jeffriess, B 2002, ‘Aquaculture – the wider issues’, Aquafest 2002, Hobart.

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Jeffriess, B 2003, ‘TBOASA R&D Report’, Southern Bluefin Tuna Industry Workshop. 3rd November, Port Lincoln. Jeffriess, B 2004, ‘TBOASA research and development report’, Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Jeffriess, B 2006, ‘SBT Industry Update: 2006’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 1-3. Jeffriess, B 2006, ‘SBT Industry updated: 2005’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005. SBT Aquaculture Subprogram, pp. 1-7. Jeffriess, B 2007, ‘Southern Bluefin Tuna Industry update’, DRAFT SBT Aquaculture Subprogram Industry Workshop Handbook 2007, SBT Aquaculture Subprogram Industry Workshop, 21st November 2007, Port Lincoln. SBT Aquaculture Subprogram, p. 9-12. Jones, E and Kaempf, J 2007, ‘Modelling the wave climate of south west Spencer Gulf, South Australia, Australia’, Australian Meteorological Oceanographic Society (AMOS) Conference, Adelaide, 5-8 February 2007. Jones, E, Kaempf, J and Fernandes, M 2007, ‘The development of a wave model for south-west Spencer Gulf, South Australia, Australia’, Waves in Shallow Environments Conference, Lorne, Victoria, April 2007. Jones, E, Kaempf, J and Fernandes, M 2007, ‘The development of a wave model for south-west Spencer Gulf, South Australia, Australia’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007. Jones, E, Kaempf, J and Fernandes, M 2008, ‘The interaction between waves and the observed sediment distribution of south-west Spencer Gulf, South Australia, Australia’, Coastal Environment 2008, UK. Jones, M, Powell, M and Carter, C 2005, ‘Metabolic scope of Atlantic salmon is reduced by disease’, FRDC Aquatic Animal Health Conference, Cairns, 26th - 28th July. Jones, M, Powell, M and Carter, CG 2005, ‘Metabolic scope of Atlantic salmon is reduced by disease, ‘European Association of Fish Pathologists Conference, Copenhagen, Denmark’, 11th - 16th September. Jones, M, Powell, M, Becker, J and Carter, C 2006), ‘The effects of gill disease on the metabolic rate of Atlantic salmon’, 12th International Symposium on Fish Nutrition and Feeding, Biarritz, France. 28th - 31st May. Jones, M, Powell, M, Becker, J and Carter, C 2007, ‘The effect of amoebic gill disease and feed deprivation on the metabolic rate of Atlantic salmon’, 4th Aquafin CRC Conference, Barossa Valley, SA, 14 -17 May. Jones, MA, Powell, MD and Carter, CG 2005, ‘Metabolic scope of Atlantic salmon is reduced by disease’, 3rd Aquafin CRC Conference, Hobart, 5-7 July. Kaempf, J 2007, ‘Coastal upwelling in the eastern Great Australian Bight’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007. Korte, A and Schuller, K 2004, ‘Development of an in vitro method for high-throughput testing of antioxidants using fish cell cultures’, Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Korte, A and Schuller, K 2007, ‘The application of cell lines for quality and nutritional studies in yellowtail kingfish and southern bluefin tuna’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007. Korte, A, Thomas, P and Schuller, K 2006, ‘Development of southern bluefin tuna primary cell cultures derived from gonad tissue and the development of an in vitro method for highthrough put testing of anti-oxidants’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005. SBT Aquaculture Subprogram, pp. 139 – 143. Korte, A, Thomas, P and Schuller, K 2007, ‘Antioxidant screen using the southern bluefin tuna cell line derived from gonad tissue’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007.

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Korte, A, Thomas, P, Williams, L, Crane, M and Schuller, K 2006, ‘Development and characterisation of southern bluefin tuna primary cell cultures derived from gonad tissue’, poster presentation, proceedings for the Aqua 2006 Conference, European Aquaculture Society and World Aquaculture Society, Florence, Italy, May, 2006. viewed . Korte, A, Thomas, P, Williams, L, Crane, M and Schuller, K 2006, ‘Development and characterisation of southern bluefin tuna primary cell cultures derived from gonad tissue’, CD-ROM, program and abstract book of the Skretting Australiasian Aquaculture Conference 2006, Adelaide. Korte, A, Williams, L, Ellis, D, Thomas, P, Crane, M and Schuller, K 2006, ‘Development of the southern bluefin tuna (SBT) gonad cell line as a platform for screening antioxidants’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 46-50. Korte, A, Williams, L, Ellis, D, Thompson, J, Hutchinson, R, Thomas, P, Crane, M and Schuller, K 2006, ‘Development and characterisation of a southern bluefin tuna (Thunnus maccoyii) cell line derived from gonad tissue’, Aqua 2006 Conference, EAS, Italy, May 2006. Kruesman, M, Adams, M, Hayward, C and Nowak, B 2006, ‘Histopathology of three metazoan gill parasites in southern bluefin tuna, Thunnus maccoyii’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005. SBT Aquaculture Subprogram, pp. 126 – 127. Lauer P, Fernandes, MB, Tanner, J, Fairweather, P and Cheshire, A 2005, ‘SBT farming and nutrient flows. European Aquaculture Conference’, Trondheim, Norway, August 5-8, 2005. Lauer, P, Fernandes, M, Fairweather, P, Tanner, J and Cheshire, A 2005, ‘Southern bluefin tuna (Thunnus maccoyii) farming and benthic fluxes in South Australia’, Aquaculture Europe. Lauer, P, Fernandes, M, Tanner, J, Fairweather, P and Cheshire, A 2004, ‘Tuna farming and the seafloor’, poster presentation, Australian Marine Sciences Association Conference Proceedings, Hobart, Tasmania. Lauer, P, Fernandes, M, Tanner, J, Fairweather, P and Cheshire, A 2004, ‘Tuna farming and the seafloor’, Australasian Aquaculture Conference Proceedings, Sydney, 26-29 September 2004. Lauer, P, Fernandes, M, Tanner, J, Fairweather, P and Cheshire, A 2004, ‘SBT farming and nutrient flows; Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Lauer, P, Fernandes, MB, Tanner, J, Fairweather, P and Cheshire, A 2005, ‘The contribution of sediments to nutrient cycles in the context of finfish aquaculture’, Aquafin CRC Conference, Conference Proceedings, p.38. Hobart, Australia, July 5-7, 2005. Lauer, PR, Tanner, J, Fairweather, P and Cheshire, A 2003, ‘Benthic respiration adjacent to tuna pontoons’, Aquafin CRC Conference, 28-30 October Adelaide, South Australia. Leef, M, Harris, J and Powell, M 2002, ‘The pathophysiology of amoebic gill disease in Atlantic salmon, Salmo salar’, Aquafin CRC Conference, Hobart, Tasmania, 22-24 September. Loo, G and Schuller, K 2007’, Expression and purification of a 2-cys peroxiredoxin gene from southern bluefin tuna (Thunnus maccoyii)’, Poster presentation, 4th Aquafin CRC Conference, Barossa Valley, SA. 14-17 May. Loo, M 2003, ‘Regional environmental assessment: A regional multi-year interpretation of una environmental monitoring and the establishment of an in-situ telemetry based monitoring system’, Southern Bluefin Tuna Industry Workshop, 3rd November, Port Lincoln. Loo, M 2007, ‘What is new in the Tuna Environmental Monitoring Program’, DRAFT SBT Aquaculture Subprogram Industry Workshop Handbook 2007, SBT Aquaculture Subprogram Industry Workshop, 21st November 2007, Port Lincoln, p. 38-48. Loo, M and Cheshire, A 2003, ‘Telemetry water quality monitoring system’, SBT Aquaculture Subprogram - Aquafin CRC - FRDC Industry Workshop, 3rd November, Port Lincoln. Loo, M and Cheshire, A 2004, ‘Development of regional environmental sustainability assessments for tuna sea cage aquaculture (RESA)’, Aquafin CRC SBT Environment Workshop, Adelaide, 10th May.

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Loo, M and Cheshire, A 2004, ‘Update on project: Development of regional sustainability assessments for tuna sea cage aquaculture’, SBT Scientific and Steering Committee Meeting, Port Lincoln, 31st May - 1st June. Loo, M and Cheshire, A 2007, ‘Oxytuna: A model of oxygen availability in a finfish sea cage’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007. Loo, M, Ophel-Keller, K, Cheshire, A, Herdina, Drabsch S, McKay, A and Hartley, D 2006 ‘Rapid environmental assessment and monitoring of tuna aquaculture using DNA – based technique’, CD-ROM, Program and abstract book of the Skretting Australiasian Aquaculture Conference 2006, Adelaide. Loo, M, Ophel-Keller, K, Hartley, D, McKay, A and Cheshire, A 2002, ‘Rapid methods for assessing the environmental effects of aquaculture’, Aquafin CRC Conference, Hobart, Tasmania, 22-24 September. Loo, M, Turner, D and Cheshire, A 2004, ‘SBT telemetry-based environmental monitoring system. In S. Clarke and J. Ham (Eds.). Southern Bluefin Tuna Aquaculture Subprogram-Aquafin CRCFRDC Industry Workshop Handbook’, Port Lincoln, Australia: SARDI Aquatic Sciences, pp. 109-114. Loo, M, Turner, D and Cheshire, A 2004, ‘SBT telemetry-based environmental monitoring system’, In: Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004, pp. 109114. McGowan, T, Hayward, C, Aiken, H, Crosbie, P and Nowak, B 2006, ‘Haemoglobin levels in southern bluefin tuna, Thunnus maccoyii, in response to time in culture and blood feeding parasite burden’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, p. 62. Middleton J 2007, ‘Ocean currents of Boston Bay: Observation’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007. Middleton, J 2006, ‘Ocean circulation off South Australia, implications for fisheries and the Integrated Marine Observing System’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, p. 9. Miller, K, Sanderson, K and Thomas, PM 2002, ‘Muscle tissue histology of wild and farmed southern bluefin tuna’, SBT Subprogram Industry Workshop, Port Lincoln, SA, 4th November. Miller, K, Thomas, P, Lewis, M, Carragher, J and Sanderson, K 2003, ‘Muscle tissue histology of farmed southern bluefin tuna’, Aquafin CRC Conference, 28-30 October Adelaide, South Australia. Miller, K, Thomas, P, Lewis, M, Carragher, J and Sanderson, K 2005, ‘Muscle tissue histology of farmed southern bluefin tuna (Thunnus maccoyii)’, RV Baudinette Memorial Symposium, October. Montague, P 2002, ‘Australian southern bluefin tuna farming and research activity –national report’, 1st International Symposium on Domestication of Bluefin Tuna, Cartagena, Spain; 3-8 February 2002. Montague, P 2002, ‘Australian southern bluefin tuna farming and research activity : national report’, First International Symposium on Domestication of Bluefin Tuna, Cartagena, Spain. 3-8 February. Mount, G, Fernandes, M and Cheshire, A 2005, ‘Polyculture as a waste management strategy for finfish aquaculture’, SBT Aquaculture Subprogram Industry Workshop, Draft Industry Handbook 2005, Port Lincoln, South Australia, 22nd November, pp.69-71. Musgrove, R, Carragher, J, Manning, A, Thomas, P and Buchanan, J 2007, ‘Nutritional profiles of baitfish: Effects of harvest and post-harvest processes on qualty of local baitfish for feeding southern bluefin tuna’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007. Musgrove, R, Carragher, J, Manning, A, Thomas, P and Buchanan, J 2007, ‘Nutritional profiles of baitfish: Effects of harvest and post-harvest processes on qualty of local baitfish for feeding southern bluefin tuna’, Poster presentation, 4th Aquafin CRC Conference, Barossa Valley, SA. 14-17 May.

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Musgrove, R, Carragher, J, Slattery, S, Manning, A, Thomas, P and Buchanan, J 2007, ‘Factors affecting rates of deterioration in Australian sardine (Sardinops sagax) quality during post harvets handling’, World Seafood Congress, Dublin, Ireland. Musgrove, RJ, Fitzgibbon, Q, Baudinette, RV and Moxham, K 2003, ‘Can’t take the tuna to the respirometer? Proposed technologies to examine southern bluefin tuna metabolic physiology’, 2nd Aquafin CRC Conference, Adelaide. Nightingale, I and Montgomery, H 2007, ‘Why plan for aquaculture?’, 4th Aquafin CRC Conference, Barossa Valley, SA, 14-17 May. Nowak B 2005, ‘Aquafin CRC Health program’, FRDC Aquatic Animal Health Subprogram Scientific Conference, Cairns, July 2005. Nowak B 2005, ‘Effects of husbandry on SBT health’, Aquafin CRC conference, Hobart July 2005. Nowak, B 2003, ‘Disease and parasites: outcomes of the SBT health surveillance project to-date’, Southern Bluefin Tuna Industry Workshop, 3rd November, Port Lincoln. Nowak, B 2001, ‘Tuna health risks’, Southern bluefin tuna Aquaculture Subprogram Industry Workshops, Port Lincoln, 13 November. Nowak, B 2002, ‘Aquafin CRC Health Program - research highlights’, Aquafest, Hobart, Tasmania, 19-22 September. Nowak, B 2002, ‘Evaluation of health risks to farmed southern bluefin tuna’, 4th International Symposium on Aquatic Animal Health, New Orleans, Louisiana, USA, 1 - 5 September. Nowak, B 2002, ‘Evaluation of health risks to the farmed southern bluefin tuna’, Aquafin CRC Conference, Hobart, Tasmania, 22-24 September. Nowak, B 2002, ‘Hazard identification - industry perception of potential health hazards in tuna farming, tuna industry risk register’, Hazard identification workshop, 24 January. Nowak, B 2002, ‘Tuna health risks – Risk register’, Southern Bluefin Tuna Aquaculture Subprogram Industry Workshops, Port Lincoln, 24 January. Nowak, B 2002, ‘Tuna health risks’, Southern Bluefin Tuna Aquaculture Subprogram Industry Workshops, Port Lincoln, 29 July. Nowak, B 2004, ‘Aquafin CRC Health Program’, Proceedings of the Australasian Aquaculture Conference, Sydney, September 2004. Nowak, B 2006, ‘Diversity of parasites in aquaculture - contrasts with wild populations’, oral presentation, Australian Parasitology Society Conference. Nowak, B 2006, ‘Parasite conditions in Australian mariculture’, Proceedings of the SCOFDA (Sustainable Control of Fish Diseases in Aquaculture) Conference, Denmark. Nowak, B and Cameron, A 2002, ‘Evaluation of health risks to the farmed southern bluefin tuna’, Southern Bluefin Tuna Aquaculture Subprogram Industry Workshops, Port Lincoln, 4 November. Nowak, B, Aiken, H and Hayward, C 2006, ‘Effect of husbandry practices on infections with gill parasites in farmed southern bluefin tuna’, CD-ROM, Proceedings of the International Symposium for Veterinary Epidemiology and Economics, Cairns. Nowak, B, Aiken, H and Hayward, C 2006, ‘Effect of longer term holding on SBT health’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 10-11. Nowak, B, Hayward, C and Aiken, H 2004, ‘Preliminary results from SBT health survey in 2004’, In: Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Nowak, B, Hayward, C, Aiken, H, Deveney, M, Bott, N, Johnston, C, Ophel-Keller, K, Crane, M, Williams, N, Korte, A, Schuller, K, Evans, D, Ellis, D, Warland, D and Clarke, S 2006, ‘Southern bluefin tuna health: risks, surveillance and science’, CD-ROM, Program and abstract book of the Skretting Australiasian Aquaculture Conference, Adelaide, 27th-30th August 2006.

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Nowak, B, Hayward, C, Aiken, H, Deveney, M, Bott, N, Johnston, C, Ophel-Keller, K, Crane, M, Williams, N, Korte, A, Schuller, K, Evans, D, Ellis, D, Warland, D and Clarke, S 2006’, Southern bluefin tuna health: Risks, surveillance and science’, SBT Aquaculture Subprogram Industry Workshop, August. Nowak, B, Mladineo, I, Oraic, D and Vittrup, C 2003, ‘Health risks in tuna culture’, 11th International Conference of the European Association of Fish Pathologists, Malta, 23rd September. Nowak, BF 2002, ‘Evaluation of health risks to the farmed southern bluefin tuna’, Proceedings 4th International Symposium on Aquatic Animal Health, September 1-5, 2002, New Orleans, USA. Nowak, BF 2003, ‘Health risks of southern bluefin tuna cultured in South Australia’, 11th International Conference of the European Association of Fish Pathologists, Malta. Nowak, BF 2004, ‘Aquafin CRC Health Program’, September 2004, Australasia Aquaculture, Sydney, 26th – 29th September. Nowak, BF 2008, ‘How healthy are farmed Southern Bluefin Tuna?’, 5th International Symposium of the Japanese Society for Fish Pathology. Nowak, BF, Aiken, H and Hayward, C 2006, ‘Effects of holding time on health of southern bluefin tuna’, Proceedings of the 5th International Symposium on Aquatic Animal Health, San Francisco, 2-6 September 2006. Nowak, BF, Aiken, HM, Bott, N, Deveney, M and Hayward, CJ 2008, ‘Parasites of farmed Southern Bluefin Tuna, Thunnus maccoyii’, ASP. Ophel-Keller, K 2004, ‘Detection of SBT pathogens in environmental samples’, In: Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Ophel-Keller, K and Loo, M, Cheshire, A, McKay, A and Hartley, D 2004, ‘Development of novel methodologies for cost effective assessment of the environmental impact of aquaculture’, In: Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004, pp. 133137. Ophel Keller, K, Loo, M, Cheshire, A, Herdina, Drabsch, S, McKay, A and Hartley, D 2005, ‘Rapid environmental assessment and monitoring of tuna aquaculture using molecular-based technique’, SBT Aquaculture Subprogram Industry Workshop, Draft Industry Handbook 2005, p.65-68. Port Lincoln, South Australia, 22nd November 2005. Ophel-Keller, K 2004, ‘Detection of SBT pathogens in environmental samples’, Southern Bluefin Tuna Aquaculture Subprogram Industry Workshop - Book of Abstracts. Port Lincoln, Australia, November, pp. 78- 79. Padula, D 2003, ‘SBT residues: present and future research directed at meeting regulatory export and import requirements’, Southern Bluefin Tuna Industry Workshop. 3rd November, Port Lincoln. Padula, D 2003, ‘Review of the 2002 residue data and 2003 preliminary data’, Food Standards Australia, Canberra, 29th October. Padula, D 2004, ‘A through-chain risk-based assessment of residues in southern bluefin tuna (Thunnus maccoyii): Predictive model development’, Australasian Aquaculture Conference, Sydney, p. 224. Padula, D 2004, ‘Japanese 2003 official import testing data on levels of dioxin in imported Australian and Mediterranean Bluefin Tuna’, In: Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Padula, D 2004, ‘Levels of dioxin (PCDD/F) and PCBs in a random sample of Australian aquacultureproduced southern bluefin tuna (Thunnus maccoyii) Organohalogen Compounds’, Dioxin Symposium, Germany, 66, pp. 2097-2102. Padula, D 2005, ‘Export of farmed SBT into China: Regulatory environment and challenges’, SBT Aquaculture Subprogram Industry Workshop, Draft Industry Handbook 2005, Port Lincoln, South Australia, 22nd November 2005, p.122.

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Padula, D 2005, ‘Recent visits to China and Vietnam in October and November 2005’, Southern Bluefin Tuna Subprogram Industry Workshop, Port Lincoln, November. Padula, D 2006, ‘Market access traceability requirements for bluefin tuna: global trends’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 56-58. Padula, D 2006, ‘Update on the new Japanese Positive List System for Maximum Residue Limits (MRLs) for bluefin tuna’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 51-55. Padula, D 2006, ‘Xenobiotic residues and contaminants in Australian aquaculture produced marine finfish’, International Conference on Environmental and Public Health Management: Aquaculture and Environment, Baptist University, Hong Kong, 7-9 December, p. 9. Padula, D 2007, ‘Trade outcomes from product integrity research into Australian cultured marine finfish, 4th Aquafin CRC Conference, Barossa Valley, SA, 14-17 May. Padula, D and Daughtry, B 2005, ‘Tuna residue project update’, Southern Bluefin Tuna Subprogram Industry Workshop, Port Lincoln, November 2005. Padula, D, Daughtry, B, Madigan, T, Phua, S and Balshaw, S 2006, ‘Residues in SA farmed southern bluefin tuna: research underpinning market access needs’, CD-ROM, Program and abstract book of the Skretting Australiasian Aquaculture Conference 2006, Adelaide. Padula, D, Kiermeier, A, Madigan, TL, Phua, STG 2005, ‘A market access guide for seafood exporters: international residues standards’, poster presentation, 6th World Congress on Seafood Safety, Quality and Trade: Balancing the Scales, Sydney, 14-16 September. Padula, D, Madigan, T, Kiermeier, A, Daughtry, B and Pointon, A 2004, ‘Levels of dioxin (PCDD/F) and PCBs in a random sample of Australian aquaculture-produced Southern Bluefin Tuna (Thunnus maccoyii)’, In: Organohalogen Compounds - Volume 66 (2004), Dioxin 2004 Symposium, Berlin, Germany, September 9th 2004, pp. 2097-2102. Padula, DJ 2005, ‘Consistent residue standards. International Association of Fish Inspectors Conference, Sydney (pre conference workshop conveyor), 11th September. Padula, DJ 2005, ‘Management of food safety hazards in farmed southern bluefin tuna to exploit market opportunities’, Yellow Sea Fisheries Research Institute, Qingdao, China, March. Padula, DJ 2005, ‘Management of food safety hazards in farmed southern bluefin tuna to exploit market opportunities’, Chinese Ministry of Agriculture, Beijing, March. Padula, DJ 2005, ‘Management of food safety hazards in farmed southern bluefin tuna to exploit market opportunities’, poster presentation, 6th World Congress on Seafood Safety, Quality and Trade, Sydney, 12th - 14th September. Padula, DJ 2005, ‘Mercury in capture based southern bluefin tuna (Thunnus maccoyii) aquaculture’, International Association of Fish Inspectors Conference, Sydney (pre conference workshop conveyor), 11th September. Padula, DJ 2006, ‘Export of farmed SBT into China: Regulatory environment and challenges’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005, Port Lincoln, SBT Aquaculture Subprogram, p. 131. Padula, DJ and Daughtry, BJ 2005, ‘Management of food safety hazards in farmed southern bluefin tuna to exploit market opportunities’, Southern Bluefin Tuna industry workshop, Port Lincoln, November. Padula, DJ, Daughtry, BJ, Madigan, TL, Phua, STG & Balshaw, S 2006, ‘Residues in SA farmed southern bluefin tuna: research underpinning market access needs’, Australasian Aquaculture 2006. Padula, DJ, Kiermeier, A, Daughtry, BJ, Madigan, TL and Pointon, AM 2004, ‘A through-chain riskbased assessment of residues in southern bluefin tuna Thunnus maccoyii: predictive model development’, In: Australasian Aquaculture Conference Proceedings, Sydney, September 2004.

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Padula, DJ, Kiermeier, A, Madigan, TL, Phua, STG 2005, ‘A market access guide for seafood exporters: international residues standards’, AgriQuality 20th Conference of Residue Chemists, Wellington, New Zealand, 5-7 October. Padula, DJ, Kiermerier, A, Madigan, TL and Pointon, AM 2004, ‘Residues: contaminant levels in southern bluefin tuna (Thunnus maccoyii)’, poster presentation, SARDI, MISA launch. Padula, DJ, Madigan, TL, Kiermeier, A, Daughtry, BJ, Pointon, AM 2005, ‘Fulfillment of market access requirements: lessons from the seafood trade’, AgriQuality 20th Conference of Residue Chemists, Wellington, New Zealand, 5-7 October. Phua, S, Daughtry, B, Davey, K, Lewis, D and Ashman, P 2006, ‘A quantitative study on the bioaccumulation of PCB TEQ levels in southern bluefin tuna (Thunnus maccoyii) farmed in South Australia’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 69-72. Phua, S, Davey, K and Daughtry, BJ 2005, ‘Framework for modelling chemical residuals of polychlorinated Dibenzo-p-dioxins, polychlorinated dibenzofurans (PCDD/Fs) and polychlorinated biphenyls (PCBs) in Australian ranched southern bluefin tuna (Thunnus maccoyii)’, poster presentation, Chemeca, Brisbane, 25th - 28th September. Phua, STG, Daughtry, BJ and Davey, KR 2006, ‘Modelling bioaccumulation of PCB TEQ levels in farmed southern bluefin tuna (Thunnus maccoyii)’, Proceedings of the 26th International Symposium on Halogenated Persistent Organic Pollutants, DIOXIN 2006 Conference, August 21 – 25, Oslo Norway, pp. 631-634, vol. 68. Phua, STG, Daughtry, BJ, Padula, DJ 2005, ‘Modelling of chemical residues in Australian marine finfish: Fat or fiction?’, AgriQuality 20th Conference of Residue Chemists, Wellington, New Zealand, 5-7 October. Phua, STG, Davey, KR and Daughtry, BJ 2005, ‘A preliminary global modelling methodology for quantifying PCDD/Fs and co-planar PCBs in Australian ranched southern bluefin tuna (Thunnus maccoyii)’, poster presentation, 6th World Congress on Seafood Safety, Quality and Trade: Balancing the Scales, Sydney, 14-16 September 2005. Phua, STG, Davey, KR and Daughtry, BJ 2006, ‘Framework for modeling chemical residuals of polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans (PCDD/Fs) and polychlorinated biphenyls (PCBs) in Australian ranched southern bluefin tuna (Thunnus maccoyii)’, Dioxin 2006 Conference. Phua, STG, Davey, KR, Daughtry, BJ 2005, ‘Framework for modelling chemical residuals of Polychlorinated Dibenzo-p-dioxins, Polychlorinated Dibenzofurans (PCDD/Fs) and Polychlorinated Biphenyls (PCBs) in Australian ranched southern bluefin tuna (Thunnus maccoyii)’, Chemical Engineering Conference Australasia (CHEMECA), 25-28 September 2005, Brisbane. Purser, J 2004, ‘Methods for measuring feeding behaviour and food intake in fish’, In: Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Putro, S, Svane, I and Robertson, J 2003, ‘Studies of the spatial and temporal structures and distributions of macrobenthic assemblages inhabiting coarse sediments under southern bluefin tuna farms’, Aquafin CRC Conference Proceedings, Adelaide, October, pp. 20-21. Rough, K, Gunn, J, Paterson, T and Hartog, J 2004, ‘Effect of feeding frequency on the growth of southern bluefin tuna Thunnus maccoyii’, poster presentation, In: Australasian Aquaculture Conference Proceedings, Sydney, September 2004. Rough, K, Loo, M, Ellis, D, de Nys, R and Padula, D 2007, ‘Management of biofouling on tuna nets’, DRAFT SBT Aquaculture Subprogram Industry Workshop Handbook 2007, SBT Aquaculture Subprogram Industry Workshop, 21st November, Port Lincoln, p. 37. Rough, K, Loo, M, Ellis, D, DeNys, R and Padula, D 2007, ‘Management of biofouling on tuna nets’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007. Rough, KM, Nowak, B and Reuter, R 2004, ‘Haematology of southern bluefin tuna Thunnus maccoyii, poster presentation, In: Australasian Aquaculture Conference Proceedings, Sydney, September 2004.

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Schuller, K 2006, ‘Potential applications of the SBT cell line in tuna nutrition, reproduction and health’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 63-64. Seymour, R, Fitzgibbon, Q, Frappell, P, Ellis, D, Carragher, J, Buchanan, J and Musgrove, R 2006, ‘Activity metabolism in live-held southern bluefin tuna (Thunnus maccoyii), Phase II’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 34-37. Smullen, R 2007, ‘Feed development and trends: A feed company perspective’, 4th Aquafin CRC Conference, Barossa Valley, SA. 14 - 17 May. Stone, D 2007, ‘Past experiences: implications for future SBT nutrition research’, DRAFT SBT Aquaculture Subprogram Industry Workshop Handbook 2007, SBT Aquaculture Subprogram Industry Workshop, 21st November 2007, Port Lincoln, pp. 16-17. Svane, I 2003, ‘Scavengers at tuna farms’, Scientific Committee Meeting, Southern Bluefin Tuna Aquaculture Subprogram, Port Lincoln, 4th November. Tanner J, Volkman, J, Blackburn, S, Bryars, S, Fernandes, M, Ganf, G, Herzfeld, M, Kaempf, J, Lewis, M, McClatchie, S, Parslow, J, Svane, I, Thompson, P, Wild-Allen, K 2005, ‘Risk & Response: The first few months and plans for the coming year’, SBT Aquaculture Subprogram Industry Workshop, Draft Industry Handbook 2005, Port Lincoln, South Australia, 22nd November, pp. 83-84. Tanner, J 2007, ‘Overview of environmental research in the tuna farming region’, 4th Aquafin CRC Conference, Barossa Valley, SA, May. Tanner, J, Middleton, J, Thompson, P and van Ruth, P 2007, ‘Risk & Response: understanding the tuna farming environment. Update on progress’, DRAFT SBT Aquaculture Subprogram Industry Workshop Handbook 2007, SBT Aquaculture Subprogram Industry Workshop, 21st November 2007, Port Lincoln, pp. 31-34. Tanner, J, Volkman, J, Blackburn, S, Bryars, S, Fernandes, M, Ganf, G, Hertzfeld, M, Kaempf, J, Lewis, M, McClatchie, S, Parslow, J, Svane, I, Thompson, P and Wild-Allen, K 2006, ‘Risk and response: The first few months and plans for the coming year’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Handbook 2005. SBT Aquaculture Subprogram, pp. 92-93. Tanner, J, Volkman, J, Blackburn, S, Bryars, S, Fernandes, M, Hertzfeld, M, Kaempf, J, Lewis, M, McClatchie, S, Margvelashvili, N, Thompson, P and Wild-Allen, K 2006, ‘Risk and Response: project progress and implications to industry’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 12-16. Tanner, J, Volkman, J, Cheshire, A, Fernandes, M, Hertzfeld, M, Loo, M, Madigan, S and Wild-Allen, K 2006, ‘Towards and integrated model of interactions between tuna aquaculture and the environment’, CD-ROM, program and abstract book of the Skretting Australiasian Aquaculture Conference 2006, Adelaide. Theil, M, Loo, M, de Jong, S, Madigan, S, Clarke, S and Tanner, J 2005, ‘Environmental risk assessment for tuna aquaculture in South Australia’, Southern Bluefin Tuna Scientific Meeting, Port Lincoln, 30th May. Thomas, P 2003, ‘Product quality evaluation from production to consumer’, Southern Bluefin Tuna Industry Workshop. 3rd November, Port Lincoln. Thomas, P 2007, ‘Aquaculture farm practices: The first step in maintaining and improving product quality’, Oceanic Institute Conference, Hawaii. Thomas, P 2007, ‘Aquafin CRC product quality research’, Nuffield Australia, Farming Scholars, Lincoln Marine Science Centre, 17 March. Thomas, P and Buchanan, J 2004, ‘Muscle concentration dose response of farmed SBT fed antioxidant fortified diets; product shelf life and sensory evaluation (preliminary results)’, In: Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Thomas, P and Buchanan, J 2006, ‘Baitfish and pelleted feeds provide dietary antioxidants to extend colour shelf life in farmed juvenile southern bluefin tuna (Thunnus maccoyii)’, Proceedings from the World Aquaculture Society Conference, World Aquaculture Society, Florence, Italy,

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May 2006, viewed . Thomas, P, Buchanan, J, Schuller, K, Carragher, J, Douglas, A, D’Antignana, T, Thompson, J, Korte, A, Chick, R and Bubner, E 2007, ‘Quality of farmed southern bluefin tuna - The odyssey’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007. Thomas, P, Buchanan, J, Schuller, K, Carragher, J, Douglas, A, Thompson, J, Korte, A, Thomas, M, Chick, R and Bubner, E 2006, ‘Maximising the control of quality in farmed southern bluefin tuna (Thunnus maccoyii): an overview’, CD-ROM, Program and abstract book of the Skretting Australiasian Aquaculture Conference 2006, Adelaide. Thomas, P, Buchanan, J, Schuller, K, Carragher, J, Douglas, A, Thompson, J, Korte, A, Thomas, M, Chick, R and Bubner, E 2006, ‘Maximising the control of quality in farmed southern bluefin tuna (Thunnus maccoyii): An overview’, SBT Aquaculture Subprogram Industry Workshop, November. Thomas, P, Buchanan, J, Thomas, M and Chick, R 2005, ‘Preliminary report on the effect of varying the fat and protein level of commercial baitfish diets on the chemical composition and quality characteristics of farmed southern bluefin tuna (Thunnus macoyii) flesh’, SBT Aquaculture Subprogram Industry Workshop, Draft Industry Handbook 2005, Port Lincoln, South Australia, 22nd November, pp. 26-34. Thomas, P, Buchanan, J, Thomas, M and Chick, R 2006, ‘Preliminary report on the effect of varying the fat and protein level of commercial baitfish diets on the chemical composition and quality characteristics of farmed southern bluefin tuna (Thunnus maccoyii) flesh’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005, SBT Aquaculture Subprogram, pp. 28 - 36. Thomas, P, Buchanan, J, Thomas, M, Bubnar, E and Chick, R 2006, ‘Making progress with dietary vitamin supplements for SBT’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 38-45. Thomas, P, Carragher, J, Bremner, A, Douglas, A, Thomas, M and Nottingham, S 2003, ‘To market to market to buy a fat tuna’, Tuna quality project sensory evaluation workshop, Aquafin CRC Conference, 28-30 October Adelaide, South Australia. Thomas, P, Carragher, J, Bremner, A, Douglas, A, Thomas, M, Buchanan, J and Nottingham, S 2004, ‘To market to buy a fat tuna - Aquafin CRC Tuna Quality Project, Sensory Evalutation Workshop’, In: Australasian Aquaculture Conference Proceedings, Sydney, September 2004. Thomas, P, D’Antignana, T, Thomas, M and Englander, R 2007, ‘Using animation to transfer information from product quality research’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007. Thomas, P, Thomas, M, McArthur, R, Schuller, K, Buchanan, J, Carragher, J, Bremner, A, Douglas, A, Thompson, J and D'Antignana, T 2004, ‘Product Quality: farm to market research on southern bluefin tuna (Thunnus maccoyii)’, poster presentation, SARDI, MISA launch. Thomas, PM 2002, ‘The effect of harvest stress on quality characteristics of southern bluefin tuna’, Aquafin CRC – southern bluefin tuna Aquaculture Subprogram, southern bluefin tuna Subprogram Industry Workshop, Port Lincoln, November. Thomas, PM and Buchanan, J 2002, ‘The effect of dietary anti-oxidants on the shelf life of southern bluefin tuna (Thunnus maccoyii)’, Aquafin CRC – southern bluefin tuna Aquaculture Subprogram, southern bluefin tuna Subprogram Industry Workshop, Port Lincoln, November. Thomas, PM and Buchanan, J 2004, ‘The effect of dietary anti-oxidants on the shelf life of pellet fed southern bluefin tuna (Thunnus maccoyii)’, Aquaculture Europe, Biotechnologies for Quality. Barcelona Spain, October 20-23. Thomas, PM and Buchanan, J 2004, ‘The effect of dietary anti-oxidants on the shelf life of pellet fed southern bluefin tuna (Thunnus maccoyii)’, In: Aquaculture Europe 2004 - Biotechnologies for Quality Conference Proceedings, Barcelona, Spain, October 20-23rd 2004. Thomas, PM and Buchanan, J 2006, ‘The use of dietary antioxidants to extend colour shelf life in farmed juvenile southern bluefin tuna (Thunnus maccoyii)’, Proceedings of the Alltech's 22nd

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International Feed Industry Symposium, Kentucky, USA , Alltech, Nottingham University Press, UK, pp. 453-460. Thomas, PM and Clancy, P 2004, ‘NIR calibration for fat and water content in farmed southern bluefin tuna’, In: Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Thomas, PM, Carragher, JF, Sanderson, K, Schuller, K, D'Antignana, T, Douglas, A, Miller, K, Thorn, W, Woods, B and Stead, K 2002, ‘Maximising the control of quality in farmed southern bluefin tuna’, Aquafin CRC – southern bluefin tuna Aquaculture Subprogram, southern bluefin tuna Subprogram Industry Workshop, Port Lincoln, November. Thomas, PT, Carragher, J, Bremner, A, Douglas, A, Thomas, M, Buchanan, J and Nottingham, S 2004, ‘To market to market to buy a fat tuna: Aquafin-CRC Tuna Quality Project, Sensory Evaluation Workshop’, Australasian Aquaculture, Sydney 26-29 September. Thompson, J 2003, ‘Oxidative stress in farmed SBT’, Southern Bluefin Tuna Industry Workshop, Port Lincoln, 3rd November. Thompson, J, Schuller, K, Thomas, P and Catcheside, D 2003, ‘Investigations into the enzymatic mechanism of protection against oxidative stress in farmed southern bluefin tuna’, Aquafin CRC Conference, 28-30 October Adelaide, South Australia. Thompson, J, Schuller, K, Thomas, P and Catcheside, D 2003, ‘Investigation into the enzymatic mechanisms of protection against oxidative stress in farmed southern bluefin tuna’, 2nd Aquafin CRC Conference, Adelaide, October. Thompson, J, Thomas, P and Schuller, K 2006, ‘Coding sequence for a glutathione peroxidase gene from southern bluefin tuna, Thunnus maccoyii’, Proceedings for the Aqua 2006 Conference, European Aquaculture Society and World Aquaculture Society, Florence, Italy, May, 2006 viewed . Thompson, J, Thomas, P, Buchanan, J and Schuller, K 2005, ‘The effects of dietary antioxidants on enzymatic and non-enzymatic protection against lipid oxidation in farmed southern bluefin tuna (Thunnus maccoyii) flesh’, SBT Aquaculture Subprogram Industry Workshop, Draft Industry Handbook 2005,. Port Lincoln, South Australia, 22nd November, pp.125-128. Thompson, J, Thomas, P, Buchanan, J and Schuller, K 2006, ‘The effects of dietary antioxidants in farmed southern bluefin tuna (Thunnus maccoyii) flesh’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Workshop Handbook 2005. SBT Aquaculture Subprogram, pp. 134 – 138. Thompson, JL, Thomas, PM and Schuller, KA 2004, ‘The role of glutathione peroxidase in maintaining flesh quality in farmed southern bluefin tuna (Thunnus maccoyii)’, Aquaculture Europe, Biotechnologies for Quality, Barcelona Spain, October 20-23. Thompson, JL, Thomas, PM and Schuller, KA 2004, ‘The role of glutathione peroxidase in maintaining flesh quality in farmed southern bluefin tuna (Thunnus maccoyii)’, In: Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Thompson, JL., Thomas, PM and Schuller, KA 2004, ‘The role of glutathione peroxidase in maintaining flesh quality in farmed southern bluefin tuna (Thunnus maccoyii)’, poster presentation, ComBio Conference Proceedings, Perth, October 2004. Thompson, P, Bonham, P, Venema, S and Eglinton, Y 2006, ‘Aquafin CRC Risk and Response Project: initial interpretation of the phytoplankton data’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 17 - 25. Thorn, W, D’Antignana, T, Thomas, PM and Schuller, K 2002, ‘Oxidative stress in southern bluefin tuna: Effects of harvest stress and storage on glutathione peroxidase activity’, Southern bluefin tuna Subprogram Industry Workshop, Port Lincoln, SA, 4th November. Tuna environment team 2003, ‘R&D workshop on quantitative DNA assays using the SARDI/CSIRO platform technologies’, Aquafin CRC-SBT Aquaculture Subprogram, Spencer Tafe Lecture Theatre, Port Lincoln, 6th November.

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Van Barneveld, Ellis, D and Vandepeer, M 2006, ‘Tuna nutrition: recent outcomes and future plans’, DRAFT SBT Aquaculture Subprogram Workshop Handbook 2006, SBT Aquaculture Subprogram, pp. 29-33. Van Barneveld, R 2004, ‘Development and commercial evaluation of tuna diets’, Southern Bluefin Tuna Aquaculture Subprogram Workshop, Port Lincoln, October. Van Barneveld, R 2004, ‘Development commercial evaluation of tuna diets’, Southern Bluefin Tuna Aquaculture Subprogram Workshop, Port Lincoln, October. Van Barneveld, R 2004, ‘Finfish nutrition’, Australasian Aquaculture Conference, Sydney (workshop conveyor), September. Van Barneveld, R 2004, ‘Recent advances in the practical nutrition of southern bluefin tuna (Thunnus maccoyii)’, Australasian Aquaculture Conference, Sydney, September. Van Barneveld, R 2004, ‘Southern bluefin tuna nutrition’, Australasian Aquaculture Conference, Sydney, September. Van Barneveld, R 2006, ‘Evolution of nutritional strategies and feeding management for farmed southern bluefin tuna, Thunnus maccoyii’, CD-ROM, Program and abstract book of the Skretting Australiasian Aquaculture Conference 2006, Adelaide. Van Barneveld, R 2006, ‘Nutritional management of farmed southern bluefin tuna’, Australasian Aquaculture Conference, Adelaide, 27-30 August. Van Barneveld, R 2006, ‘Practical feeding of pelagic fish in off shore farming systems’, Australasian Aquaculture Conference, Adelaide, 27-30 August. Van Barneveld, R, Ellis, D and Buchanan, J 2004, ‘Optimisation of farmed southern bluefin tuna (Thunnus maccoyii) nutrition to improve feed conversion efficiency and reduce production costs’, In: Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Van Barneveld, R, Ellis, D and Buchanan, J 2004, ‘Recent advances in the practical nutrition of southern bluefin tuna (Thunnus maccoyii)’, In: Australasian Aquaculture Conference Proceedings, Sydney, September 2004. Van Barneveld, R, Vandepeer, M, Ham, J and Gluis, M 2006, ‘Influence of feeding frequency and nutrient density on the growth performance and feeding efficiency of blue mackrel (Scomber australasicus) as a surrogate for southern bluefin tuna (Thunnus maccoyii)’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Handbook 2005, SBT Aquaculture Subprogram, pp. 65 - 74. Van Barneveld, RJ 2005, ‘Update of results from project 1A.4 - Development and commercial evaluaton of tuna diets’, SBT Aquaculture Subprogram Industry Workshop, November. Van Barneveld, RJ 2006, ‘Future perspectives for the nutrition of farmed bluefin tuna’, Proceedings for the Aqua 2006 Conference, European Aquaculture Society and World Aquaculture Society, Florence, Italy, May, viewed . Van Barneveld, RJ 2006, ‘Nutrition of farmed southern bluefin tuna Thunnus maccoyii for optimum production efficiency’, Proceedings for the Aqua 2006 Conference, European Aquaculture Society and World Aquaculture Society, Florence, Italy, May, 2006, viewed . Van Barneveld, RJ, Ellis, D, and Buchannan, J 2004, ‘Optimisation of farmed southern bluefin tuna (Thunnus maccoyii) nutrition to improve feed conversion efficiency and reduce production costs’, Southern Bluefin Tuna Aquaculture Subprogram (Aquafin CRC - FRDC) Industry Workshop Handbook (updated) 2004, pp. 59-67. Van Barneveld, RJ, Vanderpeer, M, Ham, J, and Glius, M 2005, ‘Influence of feeding frequency and nutrient density on the growth performance and feeding efficiency of blue mackrel (Scomber australasicus) as a surrogate for souther bluefin tuna (Thunnus maccoyii)’, Southern Bluefin Tuna Aquaculture Subprogram (Aquafin CRC - FRDC) Industry Workshop Handbook (updated) 2005, pp. 65-74.

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Van Ruth, P 2007, ‘Seasonal variability in phytoplankton abundance and community composition in the Lincoln Offshore Subtidal Aquaculture Zone’, DRAFT SBT Aquaculture Subprogram Industry Workshop Handbook 2007, SBT Aquaculture Subprogram Industry Workshop, 21st November 2007, Port Lincoln, pp. 35-36. Van Ruth, P, Thompson, P, Bonham, P, Clementson, L and Blackburn, S 2007, ‘Seasonal variability in phytoplankton abundance and community composition in the Port Lincoln tuna farming zone’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007. Volkman, J 2006, ‘Best practice in environmental management of complex ecosystems with multiple users’, Multiple Use Workshop, CSIRO, Hobart, 13 - 14 June. Volkman, J 2006, ‘The biogeochemical outputs for a possible future feed scenario’, DPIWE, Hobart, 18th May. Volkman, J 2007, ‘Aquaculture research and development in Australia’, BioEco Conference, Tianjin, China, June 2007. Volkman, J 2007, ‘Australian research to ensure sustainability in aquauculture’, Bio-Eco Conference, Tianjin, June 2007. Volkman, J.K. (2007). Australian research to ensure sustainability of aquaculture. BioEco 2007 International Conference for Bioeconomy, Tianjin, China, June 26-28. (Extended Abstracts, pp. 221-223). Volkman, J.K. (2007). Environmental research for finfish aquaculture in Australia. In Open Ocean Aquaculture – Moving Forward (Cheng-Sheng Lee and Patricia J. O’Bryen, editors). The Oceanic Institute, Hawaii, pp. 51-56. Volkman, JK 2002, ‘Environmental research for aquaculture environmental sustainable development’, Aquafest Australia, Wrest Point Convention Centre, Hobart, Australia, 19-22 September. Volkman, JK 2002, ‘Environmental research for aquaculture’, Sustainable Australian Aquaculture – Practical Solutions to Achieving ESD, Melbourne, 14-16 July. Volkman, JK 2002, ‘Finfish aquaculture and the Australian environment’, Sustainable Australian Aquaculture- Practical Solutions to Achieving ESD, Melbourne, 14-16 July. Volkman, JK 2005, ‘Environment program highlights’, 3rd Aquafin CRC Conference, Hobart, 5th - 7th July. Volkman, JK 2005, ‘Environmental risks, adaptive management and regional monitoring’, 3rd Aquafin CRC Conference, Hobart, 5th - 7th July. Volkman, JK 2005, ‘Indicators for finfish aquaculture’, Ecological Indicators Workshop, CSIRO Marine Laboratories, Hobart, 11th - 12th July. Volkman, JK 2005, ‘Marine genomics and ecological indicators’, Ecological Indicators Workshop, CSIRO Marine Laboratories, Hobart, 11th - 12th July. Volkman, JK 2006, ‘Environmental research for finfish aquaculture in Australia’, Open Ocean Aquaculture – Moving Forward, International workshop at East-West Center, Honolulu, October 21-29 2006. Volkman, JK and Lyne, V 2005, ‘Climate and seasonal changes to key environmental parameters’, 3rd Aquafin CRC Conference, Hobart, 5th - 7th July. Ward, T, Mc Leay, L, Dimmlich, WF, Rogers, PJ, McClatchie, S, Matthews, R, Kampf and van Ruth, P 2004, ‘Pelagic ecology of a northern boundary current system: effects of upwelling on the production and distribution of sardine, anchovy and southern bluefin tuna in the Great Australian Bight’, In: Southern Bluefin Tuna Industry Workshop Handbook, Port Lincoln, October 2004. Wild-Allen, K 2005, ‘Biogeochemical Model Formulation and Examples’, SBT Aquaculture Subprogram Industry Workshop, Draft Industry Handbook 2005, Port Lincoln, South Australia, 22nd November 2005, pp. 86-87.

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Wild-Allen, K 2006, ‘Biogeochemical model formulation and examples’, Aquafin CRC/FRDC SBT Aquaculture Subprogram Industry Handbook 2005, SBT Aquaculture Subprogram, pp. 95-96. Wild-Allen, K 2006, ‘Biogeochemical modelling - An update’, ASAS Research Seminar, TAFI marine Research Labs, Hobart, 8th March. Wild-Allen, K 2007, ‘Biogeochemical modelling in coastal waters’, 4th Aquafin CRC Conference, Barossa Valley, SA, May 2007. Wild-Allen, K 2007, ‘Use of a coastal biogeochemical model to design an environmental monitoring program’, International Symposium on Integrated Coastal Zone Management, ICES/CIEM, Norway. Wild-Allen, K 2007, ‘Use of a coastal biogeochemical model to design an environmental monitoring program’, poster presentation, 4th Aquafin CRC Conference, Barossa Valley, SA. 14-17 May. Woods, B, Walker, S and Thomas, PM 2002, ‘Determination of fat content of southern bluefin tuna using NIR spectroscopy’, Aquafin CRC – southern bluefin tuna Aquaculture Subprogram, southern bluefin tuna Subprogram Industry Workshop, Port Lincoln, November.

Final Reports Approved and Distributed Bott, N, Hayward, C and Ophel-Keller, K 2008, ‘Aquafin CRC – SBT Aquaculture Subprogram: detection of SBT Pathogens in environmental samples’, FRDC Final report (FRDC Project No. 2004/085). SBT Aquaculture Subprogram, 58pp. Buchanan, J 2005, ‘Aquafin CRC - Southern Bluefin Tuna Aquaculture Subprogram: service delivery and infrastructure management for projects requiring Port Lincoln based R&D support’, Aquafin CRC Project 1A.5(3) (FRDC Project No. 2002/249), SBT Aquaculture Subprogram, SARDI Aquatic Sciences, South Australia, 27pp. Buchanan, J 2007, ‘Aquafin CRC - SBT Aquaculture Subprogram: Quality and nutritional evaluation of baitfish used for tuna farming’, FRDC Final report (FRDC Project No. 2000/221.20), SBT Aquaculture Subprogram, 74pp. Clarke, SM, Ham, JM and Bushell, JJ 2005, ‘Aquafin CRC – FRDC Southern Bluefin Tuna Aquaculture Subprogram: coordination, facilitation and administration’, Final Report Aquafin CRC Project 5B.1 (FRDC Project No. 2001/250 and 1997/361), SBT Aquaculture Subprogram, SARDI Aquatic Sciences, South Australia, 33pp. Accompanying documents are provided on a COMMERCIAL IN CONFIDENCE DVD. Crane, MJ and Williams, LM 2005, ‘Aquafin CRC - FRDC - Southern Bluefin Tuna Aquaculture Subprogram: tuna cell line development and their application to tuna aquaculture health surveillance’, Aquafin CRC Project 3.2 (FRDC Project No. 2001/200), SBT Aquaculture Subprogram, SARDI Aquatic Sciences, South Australia, 44pp. Ellis, D and Rough, K 2005, ‘Aquafin CRC – SBT Aquaculture Subprogram: quality and nutritional evaluation of baitfish used for tuna farming’, Final Report Aquafin CRC Project 1A.2 (FRDC Project No. 2000/221), Tuna Boat Owners Association of South Australia, Australia, 13pp. Ellis, D and Rough, K 2005, ‘Aquafin CRC – SBT Aquaculture Subprogram: quality and nutritional evaluation of baitfish used for tuna farming’, Final Report Aquafin CRC Project 1A.2 (FRDC Project No. 2000/221), Tuna Boat Owners Association of South Australia, Australia, 39pp. COMMERCIAL IN CONFIDENCE. Fitzgibbon, Q, Frappell, P, Clark, T, Buchanan, J, Ellis, D, Carter, C, Musgrove, R, Carragher, J and Seymour, R 2008. ‘Activity metabolism in live-held southern bluefin tuna (Thunnus maccoyii), Phase 2’, FRDC Final report (FRDC Project No. 2005/200), Aquafin CRC, 123pp. Fernandes, M 2007, ‘Aquafin CRC - SBT Aquaculture Subprogram: tuna environment subproject evaluation of waste composition and waste mitigation’, FRDC Final report (FRDC Project No. 2001/103), SBT Aquaculture Subprogram, 289p. Gordon, R, Bayly, G and Foster, C 2006, ‘Commercialisation trials for a manufactured tuna feed’, FRDC Final Report (FRDC Project No. 2001/201), Aquafin CRC, 123 pp. COMMERCIAL IN CONFIDENCE.

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Hutchinson, W, Black, I and Buchanan, J 2008. Assessment of alternative platforms for southern bluefin tuna research. Part A. Benefit and cost analysis for alternative research platforms. Part B. Sea-based research platform assessment trials. FRDC Final Report (FRDC Project No. 2004/212), SARDI, 57pp. Loo, M, Ophel-Keller, K and Cheshire, A (Eds.) 2006, ‘Development of novel methodologies for cost effective assessment of the environmental impact of aquaculture’, FRDC Final Report (FRDC Project No. 2001/102), SARDI, 116 pp. COMMERCIAL IN CONFIDENCE. Musgrove, R and Fitzgibbon, Q 2006, ‘Aquafin CRC – SBT Aquaculture Subprogram: activity metabolism in live-held southern bluefin tuna (Thunnus maccoyii)’, FRDC Final Report (FRDC Project No. 2003/228), SARDI, 60 pp. Musgrove, R, Carragher, J, Manning, A, Zammit, B, Thomas, P and Buchanan, J 2007, ‘Nutritional profiles of baitfish 3: effects of harvest and post-harvest processes on quality of local baitfish for feeding SBT’, FRDC Final Report (FRDC Project No. 2004/211), SBT Aquaculture Subprogram, 74pp. Nowak, B, Aiken, H, Bott, N, Deveney, M, Johnston, C, McGowan, T and Hayward, C 2007, ‘Aquafin CRC – SBT Aquaculture Subprogram: investigations of the relationship between farming practices and SBT health’, FRDC Final Report (FRDC Project No. 2003/225), SBT Aquaculture Subprogram, 142pp. Padula, DJ, Daughtry, BJ, Balshaw, S and Phua, STG 2008, ‘Management of food safety hazards in farmed southern bluefin tuna to exploit market opportunities’, FRDC Final report (FRDC Project No. 2004/206), 125pp. Padula, DJ, Kiermeier, A, Daughtry, BJ, Madiagan, TL and Pinton, AM 2004, ‘Aquafin CRC – Southern bluefin tuna aquaculture subprogram: development and validation of baitfish sampling methods to address international residue standards for southern bluefin tuna (Thunnus maccoyii)’, Public release. Padula, DJ, Kiermeier, A, Daughtry, BJ, Madiagan, TL and Pinton, AM 2004, ‘Aquafin CRC – Southern bluefin tuna aquaculture subprogram: development and validation of baitfish sampling methods to address international residue standards for southern bluefin tuna (Thunnus maccoyii)’, Commercial in Confidence. Padula, DJ, Kiermeier, A, Daughtry, BJ, Madigan, TL and Pointon, AM 2004, ‘Aquafin CRC – SBT Aquaculture Subprogram: development and validation of baitfish sampling methods to address international residue standards for southern bluefin tuna (Thunnus maccoyii)’, SARDI. FRDC Final Report. Project No. 2003/227 (Restricted and general distribution versions). Tanner, J (Ed) 2007, ‘Aquafin CRC – Southern Bluefin Tuna Aquaculture Subprogram: tuna environment development of regional environmental sustainability’, FRDC Final Report (FRDC Project No. 2001/104), SBT Aquaculture Subprogram, 278pp. Thomas, P 2007, ‘Aquafin CRC - Southern Bluefin Tuna Aquaculture Subprogram: maximising the control of quality in farmed SBT’, FRDC Final Report (FRDC Project No. 2001/248). SBT Aquaculture Subprogram, 216pp.

Newsletters – Tuna-Briefs Aiken, H, Hayward, C and Nowak B 2004, ‘Epidemiology of blood fluke infection in southern bluefin tuna’ Tuna-brief, 2004-12, SBT Aquaculture Subprogram, 3pp. Bierman, P 2005, ‘Evaluation of the oceanographic conditions that determine nutrient dispersal in the offshore southern bluefin tuna farming zone’, Tuna-brief, 2005 – 8, SBT Aquaculture Subprogram, 5pp. Bryars, S 2007, ‘Environmental interactions of southern bluefin tuna farming off Port Lincoln’, Tunabrief, 2007- 4, SBT Aquaculture Subprogram, 6pp. Buchanan, J 2007, ‘Slashing electricity bills by developing shelf stable pellets’, Tuna-brief, 2007-8, SBT Aquaculture Subprogram, 2pp. Buchanan, J 2004, ‘Baitfish quality project update’, Tuna-brief, 2004-11, SBT Aquaculture Subprogram, 2pp.

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Buchanan, J 2004, ‘Shape really does matter’, Tuna-brief, 2004-13, SBT Aquaculture Subprogram, 2pp. Carragher J, Musgrove R, Manning A, Zammit B, Thomas P, Ellis D and Buchanan J 2006, ‘Effect of harvest and post-harvest processes on quality of local baitfish for feeding southern bluefin tuna’, Tuna-brief, 2006 – 1, SBT Aquaculture Subprogram, 5pp. Clark, T 2007, ‘Activity metabolism in live-held southern bluefin tuna (Thunnus maccoyii)’, Tunabrief, 2007-7, SBT Aquaculture Subprogram, 2pp. Clark T, Fitzgibbon Q, Taylor B, Frappell P, Seymour R, Ellis D, Carragher J and Buchanan J 2005, ‘Using heart rate to monitor the physiological condition and energy expenditure of southern bluefin tuna, Tuna-brief, 2005 – 7, SBT Aquaculture Subprogram, 2pp. Douglas, A 2004, ‘The loining and sectioning of a tuna carcass in Japan’ Tuna-brief, 2004-1, SBT Aquaculture Subprogram, 4pp. Ellis, D 2005, ‘Commercial sea-cage based tuna research farm, 2004’, Tuna-brief, 2005 – 1, SBT Aquaculture Subprogram, 4pp. Ellis, D 2005, ‘Commercial sea-cage based tuna research farm, 2005: update, Tuna-brief, 2005 – 6, SBT Aquaculture Subprogram, 6pp. Fitzgibbon, Q 2007, ‘Southern bluefin tuna and oxygen’, Tuna-brief, 2007-9, SBT Aquaculture Subprogram, 4pp. Ham, J and Clarke, S (Editors) 2004, ‘Tagging workshop’ Tuna-brief, 2004-8, SBT Aquaculture Subprogram, 6pp. Harrison, S, Robertson, J, Carragher, J and Svane, I 2005, ‘Silver Gulls and tuna farms’ Tuna-brief, 2005 – 11, SBT Aquaculture Subprogram, 7pp. Hayward, C, Aiken, H and Nowak, B 2005, ‘Effects of holding time on gill and heart parasites of southern bluefin tuna’ Tuna-Brief, 2005 – 9, SBT Aquaculture Subprogram, 3pp. Midling, KO and Aas, K 2004, ‘High velocity towing of live fish and flat-bottomed net cage for aquaculture and storage of live seafood’, Tuna-brief, 2004-5, SBT Aquaculture Subprogram, 5pp. Musgrove, R and Carragher, J 2007, ‘Taking care of baitfish’, Tuna-brief, 2007-10, SBT Aquaculture Subprogram, 3pp. Musgrove, R 2004, ‘Activity metabolism in live-held southern bluefin tuna (Thunnus maccoyii)’, Tunabrief, 2004-2, SBT Aquaculture Subprogram, 4pp. Nowak, B 2006, ‘Happy fish make prosperous farms’, Tuna-brief, 2006 – 4, SBT Aquaculture Subprogram, 2pp. Nowak, B and Aiken, H 2007, ‘What do we know about blood fluke effects of southern bluefin tuna (SBT)?’, Tuna-brief, 2007-6, SBT Aquaculture Subprogram, 4pp. Nowak, B and Hayward, C 2005, ‘The interaction of the copepod Caligus elongatus with southern bluefin tuna’, Tuna-brief, 2005 – 5, SBT Aquaculture Subprogram, 2pp. Nowak, B 2004, ‘Tuna health research and farming in Malta’, Tuna-brief, 2004-7, SBT Aquaculture Subprogram, 2pp. Nowak, B 2004, ‘Tuna industry health training, 2004’, Tuna-brief, 2004-6, SBT Aquaculture Subprogram, 3pp. Nowak, B 2004, ‘Update on the SBT Health Subprogram and upcoming events’, Tuna-brief, 2004-3, SBT Aquaculture Subprogram, 2pp. Padula, DJ 2007, ‘Market access traceability requirements for bluefin tuna: global trends’, Tuna-brief, 2007-1, SBT Aquaculture Subprogram, 3pp. Padula, DJ 2007, ‘New Zealand laboratory liaison’, Tuna-brief, 2007-3, SBT Aquaculture Subprogram, 2pp. Padula, DJ 2007, ‘Update on the new Japanese Positive List System for Maximum Residue Limits (MRLs) for bluefin tuna’, Tuna-brief, 2007-2, SBT Aquaculture Subprogram, 6pp.

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Rough, K 2005, ‘Net fouling management project: update’, Tuna-brief, 2005 – 3, SBT Aquaculture Subprogram, 5pp. Rough, K, Nowak, B and Reuter, R 2004, ‘Haematology of southern bluefin tuna’, Tuna-brief, 2004-9, SBT Aquaculture Subprogram, 3pp. Schuller, K and Crane, M 2006, ‘A continuous cell line for southern bluefin tuna – a world first’, Tunabrief, 2006 – 2, SBT Aquaculture Subprogram, 4pp. Svane, I and Barnett, J 2007, ‘Benthic scavengers at tuna farms’, Tuna-brief, 2007-5, SBT Aquaculture Subprogram, 3pp. Tanner, J 2005, ‘Deployment of oceanographic moorings for integrated hydrodynamic/biochemical models’, Tuna-brief, 2005 – 10, SBT Aquaculture Subprogram, 4pp. Tanner, J 2005, ‘Potential for remote sensing in environmental monitoring’, Tuna-brief, 2005 – 2, SBT Aquaculture Subprogram, 4pp. Thomas, M, D’Antignana, T and Thomas, P 2008, ‘Baitfish nutrient injection: a practical dietary supplement delivery system for southern bluefin tuna’, Tuna-brief, 2008 – 1, SBT Aquaculture Subprogram, 4pp. Thomas, P 2005, ‘Sampling procedure, analysis and interpretation of results; an explanation of some common analyses’, Tuna-brief, 2005 – 6, SBT Aquaculture Subprogram, 5pp. Thomas, P, Buchanan, J, Thomas, M and Chick, R 2006, ‘Fat and protein level of commercial baitfish diets affect the chemical composition and quality characteristics of farmed SBT’, Tuna-brief, 2006 – 3, SBT Aquaculture Subprogram, 5pp. Thomas, P 2004, ‘Consumer evaluation of farmed southern bluefin tuna in Port Lincoln, Tuna-brief, 2004-10, SBT Aquaculture Subprogram, 3pp. Thomas, P 2004, ‘Update of the product quality subprogram’, Tuna-brief, 2004- 4. SBT Aquaculture Subprogram, 4pp.

SBT Aquaculture Subprogram Website The SBT Aquaculture Subprogram website that was developed as part of the FRDC Project 2001/250 continued to be administered, having both historical and current outputs from the SBT Aquaculture Subprogram uploaded to the website. The website, in addition to a Public area, continued to include two login protected areas (Tuna Industry and Steering Committee areas) allowing the web site to be used much more extensively, providing industry members with access to Commercial In Confidence reports that were secured in these areas. As part of this project (2004/216) historical information, such as copies of all briefing papers and minutes from SBT Aquaculture Subprogram Steering Committee minutes, all SBT Aquaculture Subprogam final reports, in addition to current documents, were uploaded to the site providing an effective depository for all SBT Aquaculture Subprogram outputs. The website was also used as a communication tool, providing information on upcoming events, as well as copies of presentations in the Tuna Industry area, following industry workshops.

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The Environmental data in Port Lincoln (Real-time environmental data coming from the telemetry system located in the Boston Island SBT Farming Zone) remained available on a password protected site. An SBT Industry Microalgal database is nearly competition that will also be available on the site.

BENEFITS Beneficiaries: • The SBT aquaculture industry in South Australia. • The SBT and Australian sardine wild fishery in Australia. • Other aquaculture industries (e.g. yellowtail kingfish). • Suppliers and producers of services. • The regional economy of Port Lincoln and surrounding areas. This project implemented the Strategic R&D Plans for the SBT aquaculture industry, and the SBT aquaculture component of the Aquafin CRC Research Program, for the period 2004-2008. Key benefits included: 1. Providing a framework for an orderly and structured approach to R&D, including the production of the SBT Aquaculture Strategic Plan ‘Southern Bluefin Tuna Aquaculture Strategic R&D Plan – Towards 2012: Striving for a Profitable and Sustainable Future’, involving all relevant stakeholders. The review of the Strategic R&D Plan, and the consultation that occurred as part of this, provided the opportunity for all levels of industry and relevant stakeholders to be informed of R&D progress and to participate in the future direction of R&D. 2. Improved management and review of existing projects, ensuring they are in line with the changing priorities of industry. 3. Improved communication of the Subprogram process through the timely delivery of briefing papers to the Steering Committee, the annual production of the Industry Workshop Handbook, the annual production of an AOP to FRDC, delivery of project reports to Aquafin CRC and FRDC and the successful running of scientific meetings, increasing the communication between researchers and providing the opportunity to develop and discuss the direction of new research proposals as well as develop synergies within and between project groups. 4. Improved technology transfer with research results being disseminated to all relevant stakeholders through regular Tuna-briefs, the annual Industry Workshop, meetings and smaller focussed workshops.

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FURTHER DEVELOPMENT The SBT Aquaculture Subprogram ends 30th September 2008, along with Aquafin CRC. At this time the ASBTIA Research Committee will take over management of SBTSBT research and development. A new agreement between the ASBTIA and FRDC is expected to be finalised in November 2008 and will lead to the initiation of the SBT Research Council, which will operate under a new governance structure. David Ellis is the ASBTIA R&D Manager and is an appropriate contact at this time.

PLANNED OUTCOMES The SBT Aquaculture Subprogram successfully achieved the planned outcomes for this project. The Subprogram has provided: 1. An orderly and structured approach to research and development associated with SBT aquaculture so as to meet the needs of the industry and research managers/funders. The Subprogram, over the period of the project, has managed 23 projects and has been involved in all aspects of the projects in consultation with the industry, and Aquafin CRC and FRDC. The Subprogram has submitted every year, in consultation with industry, an Annnual Operating Plan to FRDC documenting activities that have occurred over the past 12 months and outlining future plans. 2. An up-to-date R&D Plan meeting the needs of industry participants. A new R&D plan ‘Southern Bluefin Tuna Aquaculture Strategic R&D Plan – Towards 2012: Striving for a Profitable and Sustainable Future’ was developed as part of this project in close consultation with all stakeholders. Project proposals were previously measured against the needs to the industry as outlined in the appropriate plan at the time ‘Southern Bluefin Tuna Aquaculture Strategic R&D Plan 2001-2006: Sustainability & Innovation for the Future’. 3. Efficient management and administration of the Subprogram achieving the specified milestones, outcomes and outputs. The Subprogram monitored the progress of each project, meeting with Principal Investigators at the two face-to-face Steering Committee meetings held each year, and provided updates in the briefing papers for all Steering Committee meetings. Research direction and progress was also discussed at the two scientific group meetings held annually. Throughout the year the Subprogram liased closely with all project Principal Investigators, dealing with any issues that arose.

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4. Efficient and coordinated use of the limited resources available for SBT aquaculture related research and development (measured by the diversity, suitability and satisfaction with the research platforms in use). During the period of this project most facilities for research using live SBT were located on commercial farms. However, short-term, smaller scale research requiring calmer waters did occur on a SARDI lease in Rotten Bay, south of Boston Island. 5. Appropriately managed communications disseminating research results (measured by the level of communication and absence of negative media issues). Research results were disseminated to industry during this project through Tuna-brief newsletters, the annual workshop (including a handbook), ad-hoc workshops and the SBT Aquaculture Subprogram website. 6. Relevant outcomes stated in the Commonwealth Agreement of the Aquafin CRC Business Planning • Efficient translation of research results into industry advantage The SBT Aquaculture Subprogram has worked closely with the SBT aquaculture industry to identify, manage and transfer outcomes from research projects. Industry-wide dissemination • Improved and innovative industry practices • Competitive advantage to participants The SBT Aquaculture Subprogram has made a signifcant effort to ensure a detailed depository of information remains available for the SBT aquaculture industry at the end of this project. All Subprogram administrative and project technical information and outcomes have been provided on the dedicated SBT Aquaculture Subprogram web site, including final reports and newsletters. A bibiography of all publications has also been included, as has a number of outputs (databases and models) and workshop and conference presentations. The independent impact assesssment by FERM, who consulted key industry representatives and researchers in carrying out their study, confirmed that significant impacts have already been realised.

CONCLUSIONS The SBT Aquaculture Subprogram has operated efficiently and effectively over the period of this project and has facilitated the development of a new administrative structure, governence procedures, a web based information depository and personnel succession planning, as the SBT aquaculture industry moves to a more dominant role in driving and managing future research. 60

Strategic R&D plans have been reviewed and updated as necessary to meet with new industry priorities. In doing this the Subprogram has delivered strongly when measured against the designated strategies and objectives, as well as outcomes. An independent analysis of the impacts of a range of projects has demonstrated significant finacial return. Industry technology transfer has also been a strong outcome of this project, with industry representatives very much involved through the Subprogram in advising on preferred communication methodologies and the form for outputs. The Subprogram has delivered well attended conferences, workshops and meetings to faciliate verbal communications; and project reports, scientific publications, handbooks and manuals, interactive models, news-letters, a comprehensive web site, and posters to facilitate written communication.

REFERENCES Clarke S and Ham J (Eds) (2008). Southern Bluefin Tuna Aquaculture (WildCapture) Strategic Plan – Towards 2012: Striving for a Sustainable and Profitable Future. Aquafin CRC FRDC Southern Bluefin Tuna Aquaculture Subprogram, 20pp. Clarke S, Ham J and Bushell J (2005). Aquafin CRC – FRDC Southern Bluefin Tuna Aquaculture Subprogram: Coordination, facilitation and administration. Montague P and Ham J (Eds) (2008). Cooperative Research Centre for the Aquaculture of Finfish Annual Report 2007 to 2008. Aquafin CRC, 131pp.

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APPENDICIES Appendix 1 – Intellectual Property (IP) There was no specific IP associated with this project. IP associated with each individual project managed through this project has been addressed through the Aquafin CRC Wind-up Agreement or through FRDC. Appendix 2 – Staff Mr Steven Clarke (SBT Aquaculture Subprogram Leader, SARDI) Ms Jane Ham (Project Officer, SARDI) Temporary project staff Ms Di Leith (Project Officer, SARDI) Ms Alison Hughes (Project Officer, SARDI) Ms Louise Handley (Aquaculture Administration Officer, SARDI) Appendix 3 – Project Outcomes and Outputs Production CRC Project No. FRDC Project No. Title:

Principal Investigator: Outputs: Achievements:

1A.9 2004/212 Aquafin CRC - FRDC - SBT Aquaculture Subprogram: assessment of alternative platforms for southern bluefin tuna research. Wayne Hutchinson - SARDI. Final Report. Developed a detailed analysis of the required system specifications, and cost benefit analysis of alternative platforms suitable for holding SBT for research, from which the best alternatives can be followed. R&D platforms considered include: (1) Industry seacages, (2) On-shore system of tanks holding smaller hatchery reared fish, (3) On-shore system of tanks holding wild-caught SBT, (4) Onshore system of tanks holding smaller hatchery reared fish, (5) An existing onshore R&D tank facility. The preferred platform was industry seacages; however this platform is critically dependent on contractual commitments by industry to carry out research trials, independent of commercial operational factors. Designed and tested a prototype sea-based platform for holding SBT and collecting faecal and waste feed samples required for research.

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CRC Project No. FRDC Project No. Title: Principal Investigator: Expected outputs: Achievements:

Expected Outcomes:

1A.11 2004/205 Aquafin CRC – Southern Bluefin Tuna Aquaculture Subprogram: longer term holding of SBT. David Ellis - ASBTIA. Final Report. Established that none of the downside risks to survival, health, flesh quality or residue status, anticipated for longterm holding, were realised. Noted that there were indications that environmental impacts would need to be carefully monitored in full-scale trials if long-term holding was to occur. The preliminary results of long-term holding in terms of carcase condition and growth rate were disappointing. Feeding behaviour, assimilation and growth are greatly affected by age cohort, time in culture and carcase condition and therefore feeding strategies and farming period need to be further investigated to ensure the highest rate of return. Complete an economic analysis to determine the full implications of the less than expected growth rates and carcass condition.

Nutrition & Feed Technology CRC Project No.

1A.3

FRDC Project No. Title:

2001/201 Aquafin CRC - SBT Aquaculture Subprogram: commercialisation trials for a manufactured tuna feed. Hagen Stehr – Stehr Group and Craig Foster - Skretting. Final Report. Achieved a world first by growing and fattening SBT fed on a pelleted feed on a commercial basis. Demonstrated commercially pellet fed SBT attained product quality and sale prices equal to or better than baitfish fed SBT. Developed methods for handling pellets and feeding tuna on a commercial scale: introduction of a vessel mounted feed conveyor, feed blower and underwater cameras. Enabled tuna farmers to make a cost benefit analysis for

Principal Investigators: Outputs: Achievements:

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baitfish and pelleted feeds. CRC Project No. FRDC Project No. Title:

Principal Investigator: Outputs:

Expected Outputs: Achievements:

1A.4 2001/249 (revised) Aquafin CRC - SBT Aquaculture Subprogram: Optimisation of farmed southern bluefin tuna (Thunnus maccoyii) nutrition to improve feed conversion efficiency and reduce production costs. Robert van Barneveld – Barneveld Nutrition. An industry manual “Practical Nutrition of Farmed Southern Bluefin Tuna (Thunnus maccoyii)”, outlining existing knowledge on tuna nutrition and explaining the theory behind nutrition and diet formulation. A literature review on identifying a surrogate fish species for SBT nutrition research. A report on the digestive physiology and microbiology of blue mackerel (Scomber australasicus) and skipjack tuna (Katsuwonus pelamis). Developed, in conjunction with FRDC Project 2000/221, Formu-bait© software; a feed formulation software program that accepts information on potential supplies of baitfish, including their cost and nutrient composition, and relates them to a stated nutritional requirement for feeding to SBT. Using this software, farmers can quickly identify the least-cost mixture of baitfish, based on what they have available, which when combined will produce a given protein and fat level as specified by them. Semi-moist manufactured feeds and manufacturing processes with enhanced binding properties and attractiveness for farmed southern bluefin tuna. Australasian Experimental Stockfeed Extrusion Centre for production of experimental volumes of manufactured semimoist feed for farmed southern bluefin tuna incorporating a Wenger X-85 extruder, a drying and cooling system, and a UAS vacuum infusion system. Final Report. Investigated the effects of different feeding strategies on the growth of SBT, suggesting that maintaining a constant and balanced supply of protein and fat, achieved the greatest length increase, the greatest weight gain and the lowest feed conversion ratios. Suggested that there did not appear to be any advantage in supplying either high protein/low fat or

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low protein/high fat diets at the beginning of the season to exploit higher intakes and potential growth of SBT, or later to influence fish condition prior to harvest. Identified that yellowtail kingfish, Seriola lalandi, was a more suitable surrogate in certain nutrition experiments than blue mackerel, Scomber australasicus. Made significant advances on the development of manufactured pelleted diets for SBT; improving consumption rates with moist pellets rather than dry, extruded flat pellets rather than extruded round pellets, and 30mm pellets rather than 60mm pellets. Attractants investigated had no effect on consumption by SBT. Digestibility (protein and energy) of the current manufactured diet was shown to vary throughout the tuna farming season. Commenced development of a shelf stable pellet, with preliminary results in yellowtail kingfish as a surrogate, indicating that one of the two preservatives trialled, “MycoCurb®”, showed no significant difference in apparent intake, specific growth rate or FCR. Trials still need to be undertaken with SBT. CRC Project No. FRDC Project No. Title:

Principal Investigator: CRC Project No. FRDC Project No. Title: Principal Investigator: Outputs:

Achievements:

1A.7 2003/228 Aquafin CRC - SBT Aquaculture Subprogram: activity metabolism in live-held southern bluefin tuna (Thunnus maccoyii). Richard Musgrove – SARDI (previously Russell Baudinette – University of Adelaide). 1A.7(2) 2005/200 Aquafin CRC - SBT Activity metabolism in live-held southern bluefin tuna (Thunnus maccoyii), Phase 2. Roger Seymour – University of Adelaide. Final Report. Mesocosm respirometer, installed in-situ into a marine farm research pontoon, and supporting methadology, that enables the oxygen consumption of commercial size actively swimming SBT to be measured. Defined SBT’s short term sub-lethal and lethal behavioural characteristics and tolerance limits to dissolved oxygen, providing important practical information for comparison to 65

in-situ observations and measurements. Recorded the metabolic cost of food ingestion for SBT – the first for any tuna species, finding that the energetic cost of food processing is twice that recorded for any other species. Showed that the lipid/energy content of the baitfish ingested has little influence on the energetic cost of processing. Measured the rates of nitrogen excretion and protein synthesis of SBT, showing that ammonia excretion rates of SBT are more than double those recorded for any other species. Recorded the free-swimming heart rate for SBT – a first for any tuna species; preliminary analysis suggests that postfeeding heart rate correlates closely with the size of the meal ingested. Provided data to other Aquafin CRC projects, incorporating the data into bioenergetic and environmental models, improving industry’s knowledge of tuna nutrition and how tuna aquaculture interacts with the environment. Product Quality CRC Project No. FRDC Project No. Title: Principal Investigator: CRC Project No. FRDC Project No. Title:

Principal Investigator: Outputs:

Achievements:

1A.2 2000/221 Nutritional Profiles of Baitfish 1; Quality and nutritional evaluation of baitfish used for tuna farming. Kirsten Rough and David Ellis - ASBTIA. 1A.2(2) 2000/221.20 Aquafin CRC - FRDC Southern Bluefin Tuna Aquaculture Subprogram: quality and nutritional evaluation of baitfish used for tuna farming project 2000/221 (extension). Jeff Buchanan - SARDI. Final Report for FRDC Projects 2000/221 and 2000/221.20. Baitfish composition database, providing nutritional profiles (proximate analysis, amino acid and fatty acid profiles) for all types of baitfish used for feed in SBT industry. Provided amino acid and fatty acid analysis for wild caught SBT. Established the level of vitamin C and E in fresh sardines (American pilchards and Australian sardines), and

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identified that the levels of these vitamins decline substantially during frozen storage, and that these lower levels could be potentially limiting to SBT growth and/or health. Extended the work done by FRDC Project 2001/249; developed a calibration for a portable NIR (Near Infra Red) machine (Cropscan 2000B NIR unit) which was sufficiently accurate (3% across all fat levels) to rapidly identify baitfish that are outside the expected range of fat content. Provided industry with the information to objectively compare different baitfish sources on the market for their nutritional value and choosing products that most cost effectively meets their feed requirements. CRC Project No. FRDC Project No. Title:

Principal Investigator: Outputs:

Achievements:

1A.10 2004/211 Aquafin CRC - FRDC - SBT Aquaculture Subprogram: nutritional profiles of baitfish 3: effects of harvest and postharvest processes on quality of local pilchards for feeding SBT. John Carragher – SARDI. Final Report. Poster for suppliers to improve post-harvest treatment of baitfish to optimise quality. Demonstrated the effects of harvest and post-harvest practices on the nutritional quality of locally-caught Australian sardines (Sardinops sagax) and of redbait (Emmelichthys nitidus nitidus). Identified that there were substantial losses in key elements of the nutritional profile (i.e. vitamins and nucleotides), associated with increases in rancidity and loss of freshness. Established that greater losses in vitamin E, quality and freshness occurred during post-harvest transport and freezing, and thawing of baitfish, prior to feeding SBT. It was also established that losses during extended frozen storage can be substantial. Recommended that baitfish should be kept on ice or refrigerated when transferred from the fishing vessel’s refrigerated seawater tanks to the factory; storage time limited; and block feeding at sea is recommended over land-based thawing as rapid thawing may reduce vitamin losses.

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Established a correlation between TBARS and remaining vitamin E, indicating that TBARS is a good indicator of antioxidant status and should be included as an analytical requirement for baitfish quality in Port Lincoln. CRC Project No. FRDC Project No. Title: Principal Investigator: CRC Project No. FRDC Project No. Title:

Principal Investigator: CRC Project No. FRDC Project No. Title: Principal Investigator: Outputs:

Expected outputs: Achievements:

2.2 2001/248 Aquafin CRC - SBT Aquaculture Subprogram: maximising the control of quality in farmed SBT. Philip Thomas – Flinders University. 2.2(2) 2004/209 Aquafin CRC - SBT Aquaculture Subprogram: dietary supplements for reducing oxidative stress and improving flesh quality attributes in SBT. Philip Thomas – Flinders University. 2.2(3) 2004/209 (extension). Baitfish extension project. Trent D’Antignana – Flinders University. Final Report 2001/248. Produced an immortal tuna cell line. The cell line has been used to test the effectiveness of antioxidant vitamin supplements in vitro. Final Report 2004/209 and 2004/209 (extension). Traced chilled SBT to the marketplace in Japan with industry responding to identified points of risk. Carried out objective measures and sensory analysis of SBT quality by Japanese and Australian researchers. Achieved correlations between Japanese and Australian assessments of SBT quality. Observed Japanese practices, enabling sensory analysis of tuna flesh to be carried out in Port Lincoln. Achieved preliminary objective colour measurement using digital camera technology, providing a good correlation with Japanese market price and quality grading. Determined the levels of vitamin C and E and selenium in wild and farmed SBT. Achieved increases in the levels of vitamin C in SBT flesh by sprinkling a vitamin mix onto baitfish, however did not find any increases in the level of vitamin E using the same method.

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Achieved higher levels of vitamin C, E and selenium in the flesh of SBT fed pellets and this resulted in significantly longer shelf life for SBT flesh. Showed that increasing the level of antioxidants in the flesh of SBT did not adversely affect human sensory qualities, and did extend the colour shelf life of fresh, and frozen and thawed (75-80% of the total product exported to Japan), SBT sashimi. Found that the quality and shelf-life stability of frozen/thawed SBT product was not dissimilar to the fresh product. Cold Wave® technology was well suited to freezing SBT and maintaining its quality. Mapped the distributions of fat, glycogen and vitamins in SBT carcasses, and their changes with season, providing information useful in determining harvest strategies, particularly if the farm cycle is extended. Assessed NIR for in-line measurement of carcase fat, as a major tool in quality grading. Established that current best commercial harvest practice, along with transport practices (a) shipped on day of harvest) and (b) shipped the following day), did not have a major effect of quality characteristics of SBT. Food Safety CRC Project No.

2.1

FRDC Project No. Title:

2003/227 Aquafin CRC - SBT Aquaculture Subprogram: development and validation of baitfish sampling methods to address international residue standards for southern bluefin tuna (Thunnus maccoyii). David Padula - SARDI. 2.1(2) 2004/206 Aquafin CRC - SBT Aquaculture Subprogram: management of food safety hazards in farmed southern bluefin tuna to exploit market opportunities. David Padula - SARDI. Final Reports 2003/227 and 2004/206. Southern Bluefin Tuna (Thunnus maccoyii) South Australian Residue Research Data 2005-2006 booklet. Guide to Residue Assessment in Feeds for southern bluefin

Principal Investigator: CRC Project No. FRDC Project No. Title:

Principal Investigator: Outputs:

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Achievements:

(Thunnus maccoyii) Sampling Protocols Baseline Data and Standards (Bi-lingual document). A new sample collection method has been adopted, which provides equivalence between data reported in this study, and those officially reported in Japan. Showed that the levels of mercury, dioxins and PCBs in wild and farmed SBT were low by international regulatory standards, regardless of the cut (akami, chu-toro, o-toro). Identified that imported baitfish were the dominant source of regulatory significant PCBs in farmed SBT. In relation to lead (Pb), aquaculture-produced tuna was not significantly different from wild-caught tuna. Performed preliminary dietary modelling of PCBs indicating that adult Japanese consumers can be assured that consumption of mixed portions of whole SBT on a regular (but not daily) basis will not incur an elevated health risk. Established that dioxins or PCB concentration of any tissue could be directly related to its fat content. Found that farmed SBT contain different types of dioxins to wild SBT; the concentration of PCBs were higher in farmed SBT than wild SBT. Performed preliminary dietary modelling of mercury indicating that consumers can eat twice as many servings of farmed SBT as wild SBT; mercury levels of wild-caught SBT decline during the first season of growth on-farm through growth-dilution. Highlighted that Japanese Government (MAFF) import testing data for wild and farmed bluefin tuna entering the Japanese domestic market indicate that Australian SBT compares favourably with overseas competitors’ product. Developed risk management (predictive) tools to assist industry in the selection of baitfish and feeding strategies that support product integrity specifications of major markets. Achieved revision of the FSANZ and Japanese Government public mercury statement in favour of SBT. Assurance of compliance with new Japanese Government regulatory standards and access to new markets including China, South Korea, United Arab Emirates and the European Union.

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Environment CRC Project No. FRDC Project No. Title:

Principal Investigator: Outputs: Achievements:

CRC Project No. FRDC Project No. Title:

Principal Investigator: Outputs:

Achievements:

4.3.1 2001/102 Aquafin CRC - SBT Aquaculture Subprogram: tuna environment subproject 1. Development of novel methodologies for cost effective assessment of the environmental impact of aquaculture. Maylene Loo (previously Anthony Cheshire) - SARDI. Final Report. Developed and validated a DNA assay system of key benthic fauna extracted from sediment samples as indicator taxa to rapidly assess the “environmental health” of the seabed in the vicinity of the tuna cages. Developed an Environmental Compliance Scorecard (ECS) system to provide a packaged set of statistical routines for analysing, integrating and summarising results from the DNA assay system and to support decision making for environmental compliance. This novel technique was adopted by the South Australian regulatory authorities for routine environmental compliance monitoring for SBT farms – a world first. Extending this technology to other SA finfish sectors (specifically yellowtail kingfish and mulloway). 4.3.2 2001/103 Aquafin CRC - SBT Aquaculture Subprogram: tuna environment subproject 2. Evaluation of waste composition and waste mitigation strategies. Milena Fernandes (previously Anthony Cheshire) - SARDI. Final Report and journal articles. Models for nutrient (phosphorus and nitrogen) inputs and outputs within tuna farms, including processes for recycling and transport. Characterised the natural sedimentary environment in the tuna farming region explaining much of the variability in terms of natural processes. Highlighted that, unlike in many other finfish aquaculture farms systems, seafloor scavengers (fish and invertebrates) can rapidly consume much of the baitfish wastes from tuna farms.

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Established the patterns and rates of recovery associated with fallowing period of tuna sites. Confirmed fallowing is a useful waste mitigation strategy and that 12 months is an adequate time for most sites to return to normal. Potential polyculture techniques for tuna farmers were reviewed and potential options highlighted. CRC Project No. FRDC Project No. Title:

Principal Investigator: Outputs:

Achievements:

4.3.3 2001/104 Aquafin CRC - SBT Subprogram: tuna environment subproject 3. Development of regional environmental sustainability assessments for tuna sea-cage aquaculture. Jason Tanner (previously Anthony Cheshire) - SARDI. Final report. Two preliminary models of carrying capacity for the tuna farming zone (1) Zone-based model to predict the likely increases in dissolved nutrients in the water column with a given level of aquaculture production (2) Carbon deposition model of the seafloor on a lease scale These models are now used by PIRSA Aquaculture to help set initial maximum stocking rates for aquaculture zones as they are revised. Provided a broad environmental risk analysis associated with tuna farming. Assessed the applicability of remote sensing for environmental monitoring in the region. Constructed and assessed a telemetry based environmental monitoring system that was used regularly by industry. Generated the first quantitative data showing that an economically important fraction of feed can be consumed by birds if appropriate measures to deter them are not taken. Demonstrated, through analysis of infauna data at control and compliance sites, that SBT farming is having an undetectable impact on the benthos at compliance sites (150m from lease boundaries), although natural geographic gradients were detected. Reviewed and documented the type and location of available environmental data-sets relevant to tuna farming.

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CRC Project No. FRDC Project No. Title:

Principal Investigator: CRC Project No. FRDC Project No. Title: Principal Investigator: Expected Outputs:

Achievements:

Expected Outcomes:

4.5 2003/226 Aquafin CRC - Southern Bluefin Tuna Aquaculture Subprogram: pilot study into the use and efficacy of antifoulants on the nets used for southern bluefin tuna (Thunnus maccoyii) culture, including residue status of tuna and the surrounding environment. Kirsten Rough - ASBTIA. 4.5 2003/226 (extension) Extension to current Aquafin CRC/FRDC SBT Projects – 2003/226. David Ellis - ASBTIA. Final Report. OxyTuna© - a model for the oxygen dynamics of a southern bluefin tuna sea-cage system which provides a quantitative prediction of the changes in oxygen concentration through time for different sea-cage configurations (cage size, net type, stocking density) in response to changes in ambient conditions (temperature, salinity, ambient dissolved oxygen, current speed). Reviewed and documented the methods available to control biofouling on nets and associated structures (using both physical and chemical means) for various marine finfish species. Determined efficacy of select antifoulant products for reducing fouling growth and their impact on net integrity using net panels in the local tuna farming environment. Identified the pattern of development of fouling communities on commercial tuna cages under current industry practices, and related this to oxygen levels monitored on the outside and inside of these nets. Using a flume tank, established a relationship between the percentage cover of fouling communities with water flow, net weight and net drag. Preliminary assessment of 2nd antifouling product with view to registration for SBT farming.

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CRC Project No. FRDC Project No. Title: Principal Investigator: Outputs:

Achievements:

4.6 2005/059 Aquafin CRC - SBT Aquaculture Subprogram. Risk and response - understanding the tuna farming environment. Jason Tanner – SARDI. Final Report. A broad-scale hydrodynamic model of Spencer Gulf and a fine scale model of the tuna farming zone at Port Lincoln describing the wave environment and currents, as well as water residence times and particle transport patterns. A fine scale model of the tuna farming zone including a biogeochemical model that provides information on dissolved nitrogen, surface chlorophyll, primary production, as well as other parameters of interest. A sediment model that relates sediment resuspension to wave energy which can be used to calculate the probability of resuspended sediment concentrations at different areas within the tuna farming zone. A set of physical and biochemical data for the tuna farming zone that can be used to calibrate the various models and to establish a baseline for the ecosystem under present farming practices. Increased understanding of nutrient cycling around tuna farms in the context of what occurs naturally without the presence of farming. An understanding of how the tuna farming region is connected to the broader Spencer Gulf, as well as shelf waters. An ability to run model scenarios to examine the consequences of altered stocking regimes, and of adverse natural events such as storms and phytoplankton blooms propagating into the area from outside.

SBT Health CRC Project No. FRDC Project No. Title:

Principal Investigator:

3.2 2001/200 Aquafin CRC - FRDC Southern Bluefin Tuna Aquaculture Subprogram: tuna cell line development and their application to tuna aquaculture health surveillance. Mark Crane – CSIRO livestock industries.

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Outputs: Achievements:

Final Report. Achieved primary (mortal) tuna cell cultures for farmed SBT. Identified the materials (e.g. culture media) and methodology (tissue explants and enzymatic dissociation) required to establish primary cell cultures; this preliminary work was instrumental in the development of the immortal cell lines achieved in FRDC Project No. 2004/209.

CRC Project No. FRDC Project No. Title:

3.1 2001/253 Aquafin CRC - SBT Aquaculture Subprogram: a risk assessment of factors influencing the health of farmed southern bluefin tuna. Barbara Nowak – University of Tasmania. 3.5 2003/225 Aquafin CRC - SBT Aquaculture Subprogram: investigation of the relationship between farming practices and southern bluefin tuna health. Barbara Nowak – University of Tasmania. 3.7 2006/225 Aquafin CRC - SBT Aquaculture Subprogram: Improving husbandry and performance of southern bluefin tuna through better understanding of the relationship of fish stress and health. Barbara Nowak – University of Tasmania. Final Report for FRDC Project 2001/253 and 2003/225. A CDROM on SBT Health distributed to SBT industry and aquaculture education providers. A password protected SBT Health web site. Best practice sampling protocols and preliminary laboratory diagnostic procedures for the SBT industry training in these techniques for the SBT industry and researchers. A SBT health surveillance program for the tuna industry.

Principal Investigator: CRC Project No. FRDC Project No. Title:

Principal Investigator: CRC Project No. FRDC Project No. Title:

Principal Investigator: Outputs:

Achievements:

Achieved greater ability to anticipate and guard against fish health related incursions in tuna farms. Identified that the current disease threat to tuna farms is negligible to low, however noted that changes to farming practices may influence the potential threat. Parasites of SBT have been identified. 75

Expected Outcomes

CRC Project No. FRDC Project No. Title: Principal Investigator: Outputs:

Achievements:

Developed partial understanding of the epidemiology of blood fluke, Cardicola forsteri. Provided a baseline for SBT microbiology by identifying bacteria associated with SBT as well as investigation of the effects of post mortem on changes in microbial populations. Potential for significant gains in SBT productivity by stress reduction. Potential for development of an integrated database for SBT industry. Improved capability to determine the health status of SBT. Improved capability to predict the performance of farmed SBT. Understanding the relationship between stress, health and performance in farmed SBT. Development of nonterminal markers for prediction of performance of farmed SBT (either individual marker or a combination of markers). 3.6 2004/085 Detection of SBT pathogens in environmental samples. Kathy Ophel-Keller - SARDI Final Report DNA probes for Uronema nigricans (ciliate), Cardicola forsteri (blood fluke) and Hexostoma thynni (gill fluke). Developed quantitative DNA tests for three key SBT parasites, Uronema nigricans (ciliate), Cardicola forsteri (blood fluke) and Hexostoma thynni (gill fluke), which can be used to quantify DNA of parasites from environmental samples such as sediment, water, sea-cage netting. Made contributions to the understanding of the biology of Uronema nigricans, Cardicola forsteri and Hexostoma thynni, and the dispersal of their larvae in the farm environment. Links to FRDC project 2003/225.

Infrastructure & Management CRC Project No. FRDC Project No.

1A.5, 1A.5 (2) and 1A.5 (3) 2000/219 & 2001/252

Title:

Aquafin CRC – Southern Bluefin Tuna Aquaculture Subprogram: Infrastructure Management, Service Delivery

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Principal Investigator: CRC Project No. FRDC Project No. Title:

Principal Investigator: CRC Project No. FRDC Project No. Title:

Principal Investigator: Outputs:

Expected outputs: Achievements:

CRC Project No. FRDC Project No. Title:

Principal Investigator: CRC Project No. FRDC Project No. Title:

and Technical Support. Jeff Buchanan - SARDI. 1A.5 2002/249 Aquafin CRC – Southern Bluefin Tuna Aquaculture Subprogram: Service delivery and infrastructure management for projects requiring Port Lincoln based R&D support. Jeff Buchanan - SARDI. 2004/205 Aquafin CRC - SBT Aquaculture Subprogram: provision of research platforms for projects requiring Port Lincoln based R&D support. David Ellis - ASBTIA. Final Report for FRDC Projects 2000/219 & 2001/252, and 2002/249. A procedures manual for SBT reserach operations in Port Lincoln. Final Report 2004/205. Delivered experimental service to project Principal Investigators utilising live SBT in a controlled and managed experimental environment. Developed methodology for designing tuna experiments that considered the logistics involved (handling, cost benefits etc.). Developed methodology to release a live white shark, which was subsequently refined and adopted by industry. Contributed to the outcomes of SBT nutrition and product quality projects by providing R&D support. 5B.1 2001/250 Aquafin CRC - Southern Bluefin Tuna Aquaculture Subprogram: implementation and coordination of research experiments conducted with caged SBT to assess manufactured diets, feeding regimes and harvesting techniques. Steven Clarke - SARDI. 5B.1(2) 2004/216 Aquafin CRC - SBT Aquaculture Subprogram:

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Principal Investigator: Outputs:

Expected outputs:

Achievements:

coordination, facilitation and administration. Steven Clarke - SARDI. Final Report (2001/250) Password protected SBT Aquaculture Subprogram website (www.sbtuna.com) that was regularly updated, providing all SBT Aquaculture Subprogram material (reports, tunabriefs, Annual Operating Plan, Strategic Plans etc.) available to industry and researchers. Southern Bluefin Tuna Aquaculture Strategic Plan R&D Plan 2001 – 2006: Sustainability & Innovation for the future. Southern Bluefin Tuna Aquaculture Strategic R&D Plan Towards 2012: Striving for a Profitable & Sustainable Future (the currently document focuses only on aquaculture and will be updated to incorporate the wild sector in the near future). 58 Tuna-brief newsletters providing research outcomes to industry. Final Report (2004/216). A micro-algal database available on the SBT Aquaculture Subprogram website, containing historical and current micro-algal data from the tuna farming region in Port Lincoln. Achieved an orderly and structured approach to research and development (R&D) associated with SBT aquaculture, involving a high level of industry involvement. Provided a means to use limited resouces available for SBT aquaculture related to R&D in an efficient and coordinated way. Managed and coordinated the delivery of outcomes for all projects within the Subprogram, including milestone reports, media releases, final reports and dissemination of research results to industry through production of regular Tuna-briefs and Industry Handbooks as well as the organisation of regular meetings and workshops.

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