Full List

Full list of publications (in peer reviewed journals only) De Palma M and Lewis CE. (2011). Macrophages limit chemotherapy. Nature 472: 303-304

Welford AF, Biziato D, Coffelt SB, Nucera S, Fisher M, Pucci F, Di Serio C, Naldini L, *De Palma M, *Tozer GM and *Lewis CE. (2011). TIE2Expressing Macrophages Limit the Therapeutic Efficacy of the Vascular Disrupting Agent, Combretastatin A4 Phosphate. J. Clin. Invest. 121:196973. Lewis CE and Ferrara N. (2011). Multiple Effects of Angiopoietin-2 Blockade on Tumors. Cancer Cell. 19: 431-3. Owen MR, Stamper IJ, Muthana M, Richardson GW, Dobson J, Lewis CE, Byrne HM. (2011). Mathematical modelling predicts synergistic antitumor effects of combining a macrophage-based, hypoxia-targeted, gene therapy with chemotherapy. Cancer Res. 71:2826-37. Tazzyman S, Barry ST, Ashton S, Wood P, Blakey D, Lewis CE, Murdoch C. (2011). Inhibition of neutrophil infiltration into A549 lung tumors in vitro and in vivo using a CXCR2-specific antagonist is associated with reduced tumor growth. Int J Cancer. Feb 15. Doi: 10.1002/ijc.25987. [Epub ahead of print]. Muthana M, Giannoudis A, Scott SD, Fang H-Y, Coffelt SB, Morrow FJ, Murdoch M, Burton JL, Cross N, Burke B, Mistry R, Hamdy FC, Brown NJ, Georgopoulos L, Hoskin P, Essand M, Lewis CE* & Maitland NJ.* (2011). Use of Macrophages to Target Therapeutic Adenovirus to Human Prostate Tumors. Cancer Res. 71:1805-15. [* joint senior authors]. Coffelt SB, Chen YY, Muthana M, Welford AF, Tal AO, Scholz A, Plate KH, Reiss Y Murdoch C, De Palma M and Lewis CE. (2011). Angiopoietin-2 stimulates Tie2-expressing monocytes to suppress T cell activation and promote regulatory T cell expansion. J. Immunol. 186:418390. Coffelt SB, Tal OA, Scholz A, De Palma M, Patel S, Urbich C, Biswas SK, Murdoch C, Plate K, Reiss Y and Lewis CE. (2010). Angiopoietin-2 regulates gene expression in Tie2-expressing monocytes and augments their inherent pro-angiogenic functions. Cancer Res. 70:5270-80. Coffelt SB, Lewis CE, Naldini L, Brown JM, Ferrara N and De Palma M. (2009). Elusive identities and overlapping phenotypes of pro-angiogenic myeloid cells in tumors. Am. J. Pathol. 176:1564-76. Fang H-Y, Hughes R, Murdoch C, Coffelt S, Biswas SK, Harris AL, Johnson RS, Imityaz HZ, Simon MC, Fredlund E, Greten R, Rius J & Lewis CE. (2009). Hypoxia inducible factors 1 and 2 are important

transcriptional effectors in primary macrophages experiencing sustained hypoxia Blood 114:844-59. Hagemann T, Biswas SK, Lawrence T, Sica A & Lewis CE. (2009). Regulation of macrophage function in tumors: the multifaceted role of NFkB. Blood 113:3139-46 Coffelt SB, Hughes R & Lewis CE. (2009). Tumor-associated macrophages: effectors of angiogenesis and tumor progression. Biochim Biophys Acta 1796:11-8. Ryan RM, Green J, Williams PJ, Tazzyman S, Hunt S, Harmey JH, Kehoe SC & Lewis CE (2009). Bacterial delivery of a novel cytolysin to hypoxic areas of solid tumors. Gene Therapy 16:329-39. Tazzyman S, Lewis CE, Murdoch C. (2009). Neutrophils: key mediators of tumour angiogenesis. Int J Exp Pathol. 90:222-31. Murdoch C, Muthana M, Coffelt S & Lewis CE. (2008). The Role of Myeloid Cells in the Promotion of Tumour Angiogenesis. Nature Reviews Cancer 8:618-31. Muthana M, Scott SD, Farrow N, Morrow F, Murdoch C, Grubb S, Brown NJ, Dobson J & Lewis CE. (2008). A novel magnetic approach to enhance the efficacy of cell-based gene therapies. Gene Therapy 15, 902–910. Pahler JC, Tazzyman S, Erez N, Chen YY, Murdoch C, Nozawa H, Lewis CE & Hanahan D. (2008). Plasticity in tumor-promoting inflammation: impairment of macrophage recruitment evokes a compensatory neutrophil response. Neoplasia 10:329-40. Larsen M, Tazzyman S, Lund E, Junker N, Lewis CE, Kristjansen PEG & Murdoch C. (2008). Hypoxia-induced secretion of macrophage migration inhibitory factor from MCF-7 breast cancer cells is regulated in a hypoxiainducible factor-independent manner. Cancer Lett. 265(2):239-49. Biswas SK, Sica A & Lewis CE. (2008). Plasticity of Macrophage Function during Tumor Progression: Regulation by Distinct Molecular Mechanisms. J. Immunol. 180:2011-2017. De Palma M, Murdoch C, Venneri MA, Naldini L & Lewis CE. (2007). Tie2-expressing Monocytes: Regulation of Tumor Angiogenesis and Therapeutic Implications. Trends Immunol. 28(12):545-50 Lewis CE, De Palma M and Naldini L. (2007). Tie2-expressing Monocytes and Tumor Angiogenesis: Regulation by Hypoxia and Angiopoietin-2. Cancer Res. 67(18):8429-32.

Murdoch C, Tazzyman S, Webster S and Lewis CE. (2007). Expression of Tie-2 by human monocytes and their responses to angiopoietin-2. J. Immunol. 178:7405-11. Lewis CE and Hughes R. (2007). Microenvironmental factors regulating macrophage function in breast tumours: hypoxia and angiopoietin-2. Breast Cancer Research 9:209-213. Webb SD, Owen MR, Byrne HM, Murdoch C, Lewis CE. (2007). Macrophage-Based Anti-Cancer Therapy: Modelling Different Modes of Tumour Targeting. Bull. Math Biol. 69:1747-76. Staton CA, Stribbling SM, García-Echeverría C, Bury JP, Tazzyman S, Lewis CE and Brown NJ. (2007). Identification of key residues involved in mediating the in vivo anti-tumour/anti-endothelial activity of Alphastatin. J. Thromb & Haemost 5:846-54. Lewis CE & Pollard J. (2006). Function of macrophage subpopulations in distinct microenvironments of tumors. Cancer Res. 66:605-12. Bingle L, Lewis CE, Corke KP, Reed MW and Brown NJ. (2006). Macrophages promote angiogenesis in human breast tumour spheroids in vivo. Br J Cancer 94:101-7. Macrae EJ, Giannoudis A, Ryan R, Brown NJ, Hamdy FC, Maitland N and Lewis CE. (2006). Gene therapy for prostate cancer: Current strategies and new cell-based approaches. Prostate 66(5):470-94. Ryan RM, Green J and Lewis CE. (2006). Use of bacteria in anti-cancer therapies. Bioessays 28:84-94. Brockington A, Wharton S, Fernando M, Gelsthorpe C, Baxter L, Ince P, Lewis CE and Shaw PG. (2006). Expression of VEGF and its receptors in the central nervous system in amyotrophic lateral sclerosis. J. Neuropath. & Exp. Neurol. 65:26-36. Fernando MS, Simpson JE, Matthews F, Brayne C, Lewis CE, Barber R, Kalaria RN, Forster G, Esteves F, Wharton SB, Shaw PJ, O'Brien JT, Ince PG. (2006). White matter lesions in an unselected cohort of the elderly. molecular pathology suggests origin from chronic hypoperfusion injury. Stroke. 37:13918. Murdoch C, Muthana M and Lewis CE. (2005). Hypoxia regulates macrophage function in inflammation. J. Immunol. 175(10):6257-63. Lewis CE and Murdoch C. (2005). Macrophage responses to hypoxia: implications for tumor progression and anti-cancer therapies. Am. J. Pathol. 167: 627-635.

Murdoch C and Lewis CE. (2005). Macrophage migration and gene expression in response to tumour hypoxia. Int. J. Cancer 117(5):701-8. Staton CA and Lewis CE. (2005). Angiogenesis inhibitors found within the haemostasis pathway. J Cell Mol Med. 9(2):286-302. Franks SJ, Byrne HM, Underwood JC and Lewis CE. (2005). Biological inferences from a mathematical model of comedo ductal carcinoma in situ of the breast. J Theor Biol. 232:523-43. Brockington A, Kirby J, Eggitt D, Schofield E, Morris C, Lewis CE, Ince PG and Shaw PJ. (2005). Screening of the regulatory and coding regions of vascular endothelial growth factor in amyotrophic lateral sclerosis. Neurogenetics. 6:101-4. Murdoch C, Giannoudis A and Lewis CE. (2004). Mechanisms regulating the recruitment of macrophages into hypoxic areas of tumors and other ischemic tissues. Blood 104:2224-34. Staton CA, Brown NJ, Rodgers GR, Corke KP, Tazzyman S, Underwood JCEU and Lewis CE. (2004). Alphastatin, a 24 amino acid fragment of human fibrinogen, is a potent new inhibitor of activated endothelial cells in vitro and in vivo. Blood 103:601-6. Ameri K, Lewis CE, Raida M, Sowter H, Hai T and Harris AL. (2004). Anoxic induction of ATF-4 via HIF-1 independent pathways of protein stabilisation in human cancer cells. Blood 103: 1876-82. Staton CA, Stribbling SM, Tazzyman S, Hughes R, Brown NJ and Lewis CE. (2004). Current methods for assaying angiogenesis in vitro and in vivo. Int J Exp Pathol. 85(5):233-48. Owen MR, Byrne HM and Lewis CE. (2004). Mathematical modelling of the use of macrophages as vehicles for drug delivery to hypoxic tumour sites. J Theor Biol. 226:377-91. Peters CL, Morris CJ, Mapp PI, Blake DR, Lewis CE and Winrow VR. (2004). The transcription factors hypoxia-inducible factor 1alpha and Ets-1 colocalize in the hypoxic synovium of inflamed joints in adjuvant-induced arthritis. Arthritis Rheum. 50:291-6. Daly ME, Makris A, Reed M and Lewis CE. (2003). Hemostatic regulators of tumor angiogenesis: A source of anti-angiogenic agents for cancer treatment? J. Natl. Cancer Inst. 95(22):1660-73. Burke B, Giannoudis A, Corke KP, Gill D, Wells M, Ziegler-Heitbrock L and Lewis CE. (2003). Hypoxia-induced gene expression in human macrophages: implications for ischemic tissues and hypoxia-regulated gene therapy. Am. J. Pathol 163(4):1233-43.

Franks SJ, Byrne HM, Mudhar HS, UnderwoodJCE and Lewis CE. (2003). Mathematical modelling of comedo ductal carcinoma in situ of the breast . Math. Med. & Biol. 20: 277-308. Staton CA, Brown NJ and Lewis CE. (2003). The role of fibrinogen and related fragments in tumour angiogenesis and metastasis. Expert Opin Biol Ther. 3(7):1105-20. Franks SJ, Byrne HM, King JR, Underwood JCE and Lewis CE. (2003). Modelling the early growth of ductal carcinoma in situ of the breast. J. Math. Biol. 47:424-52. Breward CJ, Byrne HM and Lewis CE. (2003). A multiphase model describing vascular tumour growth. Bull. Math. Biol. 65: 609-40. Ameri K, Burke B, Lewis CE and Harris AL (2002). Regulation of a rat VL30 element in human breast cancer cells in hypoxia and anoxia: role of HIF-1. Brit. J. Cancer 87, 1173 - 1181. Brown NJ, Staton CA, Rodgers GR, Corke KP, Underwood JCE and Lewis CE. (2002). Fibrinogen E fragment selectively disrupts the vasculature and inhibits the growth of murine tumours in a syngeneic murine model. Brit. J. Cancer 86: 1813-16. Burke B, Sumner S, Maitland N and Lewis CE. (2002). Macrophages in gene therapy: cellular delivery vehicles and in vivo targets. J. Leuk. Biol. 72: 417428. Breward CJ, Byrne HM and Lewis CE. (2002). The role of cell-cell interactions in a two-phase model for avascular tumor growth. J. Math. Biol. 45:125-152. Bingle L, Brown NJ and Lewis CE. (2002). The role of tumour-associated macrophages in tumour progression: implications for new anticancer therapies. J. Pathol. 196: 254-265. Burke B, Ameri K, Mahon PC, Harris AL and Lewis CE. (2002). Macrophages in gene therapy. In: ‘The Macrophage’. 2nd edition. In Burke B and Lewis CE (Eds). Oxford University Press, Oxford, U.K (in press). Burke B, Tang N, Corke KP, Tazzyman D, Ameri K, Wells M and Lewis CE. (2002). Expression of HIF-1alpha by human macrophages: implications for the use of macrophages in hypoxia-regulated cancer gene therapy. J Pathol. 196:204-12. Kelly CE, Leek RD, Byrne HM, Cox SM, Harris AL and Lewis CE. (2002). Modelling macrophage infiltration into avascular tumours. J. Theor. Med. 4:21-38.

Breward CJW, Byrne HM and Lewis CE (2001). Modelling the interactions between tumour cells and a blood vessel in a microenvironment within a vascular tumour. Euro. J. Appl. Math. 12: 529-556. Bootle-Wilbraham CA, Tazzyman S, Thompson WD, Stirk CM and Lewis CE. (2001). Fibrin fragment E stimulates the proliferation, migration and differentiation of human microvascular endothelial cells in vitro. Angiogenesis 4:269-275. Hehlgans T, Seitz C, Lewis CE and Mannel. (2001). Hypoxic upregulation of TNF receptor type 2 expression involves NF-IL-6 and is independent of HIF-1 or HIF-2. J. Interferon Cytokine Res. 21(9):757-62. Crowther M, Brown NJ, Bishop EB and Lewis CE. (2001). Microenvironmental influence on macrophage regulation of angiogenesis in wounds and malignant tumors. J. Leukoc. Biol. 70: 478-490. Donovan D, Brown NJ, Bishop ET and Lewis CE. (2001). Comparison of three in vitro human ‘angiogenesis’ assays with capillaries formed in vivo. Angiogenesis 4(2):113-21. Hollander AP, Corke KP, Freemont AJ and Lewis CE. (2001). Expression of HIF-1alpha by macrophages in the rheumatoid synovium: implications for targeting therapeutic genes to the inflamed joint. Arthrtitis & Rheumat. 4(7):1540-4. Gammack D, Byrne HM and Lewis CE (2001). Estimating the selective advantage of mutant p53 tumour cells to repeated round of hypoxia. Bull. Math. Biol. 63:135-166. Ferreux E, Lewis CE, Perrett CW and M. Wells (2001). Regulation of angiogenesis in ovarian cancer: role of p53, vascular endothelial growth factor and hypoxia. In: 'Ovarian cancer'. (Eds: I.Jacobs, C.N.Hudson, J.Shepherd, D.Oram, A.Blackett, D.Luelsey, A.Berchuck), Oxford University Press, Oxford, UK Bootle-Wilbraham CA, Tazzyman S, Marshall JM and Lewis CE (2000). Fibrinogen E-fragment inhibits the migration and tubule formation of human dermal microvascular endothelial cells in vitro. Cancer Res. 60: 4719-24. Lewis J, Landers R, Underwood JCE, Harris AL and Lewis CE. (2000). Expression of VEGF by macrophages is upregulated in avascular areas of breast carcinomas. J. Pathol. 192:150-8. Griffiths L, Binley K, Iqball S, Kan O, Maxwell P, Lewis CE, Harris AL, Kingsman S. and Naylor S. (2000). The macrophage- a novel system to deliver gene therapy to pathological hypoxia. Gene Therapy 7:255-62. Burke B, Lewis JS and Lewis CE. (2000). Cytokine production by individual cells. In: Cytokine Cell Biology. F.R. Balkwill (Ed). Oxford University Press,

Oxford. Leek RD, Hunt NC, Royds JR, Landers RJ, Lewis CE and Harris AL (2000). Macrophage infiltration is associated with VEGF and EGFR expression in breast cancer. J. Pathol. 190:430-36. Lewis JS, Lee J, Underwood JCE, Harris AL and Lewis CE. (1999). Macrophage responses to hypoxia: implications for disease mechanisms. J. Leuk. Biol. 66:889-900. Leek RD, Landers RJ, Harris AL and Lewis CE (1999). Necrosis correlates with high vascular density and focal macrophage infiltration in invasive carcinoma of the breast. Brit. J. Cancer 79:991-5. Zhang L, Scott PA, Turley H, Leek R, Lewis CE, Gatter KC, Harris AL, Mackenzie IZ, Rees MC, Bicknell R. (1998). Validation of anti-vascular endothelial growth factor (anti-VEGF) antibodies for immunohistochemical localization of VEGF in tissue sections: expression of VEGF in the human endometrium. J. Pathol. 185:402-8. Royds JR, Quarstrom E, Dower S and Lewis CE (1998). Response of tumour cells to hypoxia: role of p53 and NFkB. Mol. Pathology. 51: 55-61. Leek RD, Landers RJ, Fox SB, Ng F, Harris AL and Lewis CE (1998). Association of TNF alpha and its receptors with thymidine phosphorylase expression in invasive breast carcinoma Brit. J. Cancer 77:2246-51. Leek RD, Lewis CE, Whitehouse R and Harris AL. (1997). Association of macrophage infiltration with angiogenesis and prognosis in invasive breast carcinoma. Cancer Res. 56: 4625-9. Lewis CE, Leek RD, Harris AL and McGee JO'D. (1995). Cytokine regulation of angiogenesis in breast tumours: the role of macrophages. J. Leuk. Biol. 57: 747-51. O'Sullivan C and Lewis CE (1994). Tumour-infiltrating leukocytes : friend or foe in breast carcinoma. J. Pathol. 172 : 2292-35. Leek RD, Harris AL and Lewis CE (1994). Cytokine networks in solid human tumours: regulation of angiogenesis. J. Leuk. Biol. 56 : 423-35. O'Sullivan C, Lewis CE, Harris AL and McGee JO (1993). Secretion of epidermal growth factor by macrophages associated with breast carcinoma. Lancet. 342(8864):148-9. Pusztai L, Lewis CE and McGee JO. (1993). Epidermal growth factor, insulin-like growth factor-1 and basic fibroblast growth factor modulate the cytostatic effect of tumour necrosis factor-alpha on the breast cancer cell line, T47D. Cytokine. 5(2):169-72.

Pusztai L, Lewis CE, Lorenzen J and McGee JO. (1993). Growth factors: regulation of normal and neoplastic growth. J. Pathol.;169(2):191-201. Pusztai L, Lewis CE and McGee JO. (1993) Growth arrest of the breast cancer cell line, T47D, by TNF alpha; cell cycle specificity and signal transduction. Br J Cancer. 67(2):290-6. Baigrie RJ, Lewis CE, Lamont PM, Morris PJ and McGee JO. (1992). Effect of major surgery on the release of interferon gamma by peripheral blood mononuclear cells: an investigation at the single cell level using the reverse haemolytic plaque assay. Cytokine 4(1):63-5. Lorenzen J, Lewis CE, McCracken D, Horak E, Greenall M and McGee JO. (1991). Human tumour-associated NK cells secrete increased amounts of interferon-gamma and interleukin-4. Br J Cancer. 64(3):457-62. Lewis CE. (1991). Detecting cytokine production at the single-cell level. Cytokine. 3(3):184-8. Lewis CE, McCracken D, Ling R, Richards PS, McCarthy SP and McGee JO. (1991). Cytokine release by single, immunophenotyped human cells: use of the reverse hemolytic plaque assay. Immunol Rev. 119:23-39. Lewis CE, Ramshaw AL, Lorenzen J and McGee JO. (1991). Basic fibroblast growth factor and interleukins 4 and 6 stimulate the release of IFN-gamma by individual NK cells. Cell Immunol. 132(1):158-67. Lewis CE, McCarthy SP, Lorenzen J, McGee JO. (1990). Differential effects of LPS, IFN-gamma and TNF alpha on the secretion of lysozyme by individual human mononuclear phagocytes: relationship to cell maturity. Immunology. 69(3):402-8. Lewis CE, McCarthy SP, Richards PS, Lorenzen J, Horak E and McGee JO. (1990). Measurement of cytokine release by human cells. A quantitative analysis at the single cell level using the reverse haemolytic plaque assay. J. Immunol Methods.;127(1):51-9. McCarthy SP, Lewis CE and McGee JO. (1990). Effects of ethanol on human monocyte/macrophage lysozyme storage and release. Implications for the pathobiology of alcoholic liver disease. J. Hepatol. 10(1):90-8. Lewis CE, McCarthy SP, Lorenzen J, McGee JO. (1989). Heterogeneity among human mononuclear phagocytes in their secretion of lysozyme, interleukin 1 and type-beta transforming growth factor: a quantitative analysis at the single-cell level. Eur J Immunol. 19(11):2037-43. Lewis CE, Richards PS and Morris JF. (1989). Heterogeneity of responses to LH-releasing hormone and phorbol ester among rat gonadotrophs: a study using a reverse haemolytic plaque assay for LH. J. Mol. Endocrinol. 2(1):5563.

Lewis CE, Clark A, Ashcroft SJ, Cooper GJ and Morris JF. (1988). Calcitonin gene-related peptide and somatostatin inhibit insulin release from individual rat B cells. Mol Cell Endocrinol. 57(1-2):41-9. Cooper GJ, Willis AC, Reid KB, Clark A, Baker CA, Turner RC, Lewis CE, Morris JF, Howland K and Rothbard JB. (1987). Diabetes-associated peptide. Lancet. 24;2(8565):966. Clark A, Cooper GJ, Lewis CE, Morris JF, Willis AC, Reid KB and Turner RC. (1987). Islet amyloid formed from diabetes-associated peptide may be pathogenic in type-2 diabetes. Lancet 2(8553):231-4. Lewis CE, Megson A, Morris JF and Charlton HM. (1986). Multiple injections of LH-releasing hormone into hypogonadal (hpg) mice induce the appearance of two morphologically distinct populations of gonadotrophs. J. Endocrinol. 111(3):483-93. Lewis CE, Megson A, Morris JF and Charlton HM. (1986). Multiple injections of LH-releasing hormone into hypogonadal (hpg) mice induce the appearance of two morphologically distinct populations of gonadotrophs. J Endocrinol. 111(3):483-93. Lewis CE, Morris JF, Fink G and Johnson M. (1986). Changes in the granule population of gonadotrophs of hypogonadal (hpg) and normal female mice associated with the priming effect of LH-releasing hormone in vitro. J Endocrinol. 109(1):35-44. Lewis CE, Morris JF and Fink G. (1985). The role of microfilaments in the priming effect of LH-releasing hormone: an ultrastructural study using cytochalasin B. J Endocrinol. 106(2):211-8.