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Medical Journal of Babylon – 2007 Volume 4 No .1-2
A Clinico-bacteriological Study of the Interrelation of Corynebacterium urealyticum and Struvite Urolithiasis Ifad kerim Alshibly
Mohammed Sabri Abdulrazzak
Dept. of Microbiology College of MedicineBabylon University
MJ B
Abstract Three hundred twenty urine samples were collected from patients suffering urinary tract affection.Only alkaline sample were selected which formed 86 specimens yielded 8 isolates of Corynebacterium urealyticum . All the 8 isolates were identical regarding cultural characteristics and biochemical reactions. Results of the studied virulence factors expression by Corynebacterium urealyticum showed that urease was the most important factor in pathogenicity . An experimental study was created to assess the ability Struvite stone formation by Corynebacterium urealyticum during its growth in human urine , it was found that there was gradual increase in urine pH with associated elevation of ammonium ion concentration accompanied by bacterial growth and struvite crystals which appeared grossly as a white precipitate at the bottom of the testing tube after an overnieght incubation in human urine . this observation was compared to the failure of E.coli and control urine to increase the urine pH or ammonium concentration in sterile urine specimens , together with the inability form stuvite crystals when incubated under similar experimental conditions . The effects of the proton – pump inhibitors : omeprazole and lansoprazole on growth and pathogenicity of Corynebacterium urealyticum were examined , it was obvious that these compound markedly affected urease activity in a dose -dependent manner and the bacterial growth was significantly (p
