Page 1 of 36 Articles in PresS. Am J Physiol Endocrinol Metab (July 5, 2006). doi:10.1152/ajpendo.00097.2006
Gender differences in hormone-sensitive lipase expression, activity and phosphorylation in skeletal muscle at rest and during exercise Carsten Roepstorff1, Morten Donsmark2, Maja Thiele1, Bodil Vistisen1, Greg Stewart3, Kristian Vissing4,5, Peter Schjerling4,6, D. Grahame Hardie3, Henrik Galbo7, and Bente Kiens1 1
The Copenhagen Muscle Research Centre, Dept. of Human Physiology, Institute of Exercise and
Sport Sciences, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. 2
The Copenhagen Muscle Research Centre, Dept. of Medical Physiology, Panum Institute,
University of Copenhagen, DK-2200 Copenhagen N, Denmark. 3
Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dundee DD1
5EH, Scotland, United Kingdom. 4
The Copenhagen Muscle Research Centre, Dept. of Molecular Muscle Biology, Rigshospitalet,
DK-2100 Copenhagen Ø, Denmark. 5
Dept. of Sport Science, University of Aarhus, DK-8200 Aarhus N, Denmark.
6
Dept. of Medical Biochemistry and Genetics, University of Copenhagen, DK-2200 Copenhagen N,
Denmark. 7
The Copenhagen Muscle Research Centre, Dept. of Rheumatology, Bispebjerg Hospital, DK-2400
Copenhagen NV, Denmark. Running head: Gender difference in regulation of HSL Correspondence: Carsten Roepstorff The Copenhagen Muscle Research Centre Department of Human Physiology Institute of Exercise and Sport Sciences Universitetsparken 13 DK-2100 Copenhagen Ø Denmark Phone: + 45 35 32 16 18 Fax: + 45 35 32 16 00 E-mail:
[email protected]
Copyright © 2006 by the American Physiological Society.
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ABSTRACT Women have been shown to use more intramuscular triacylglycerol (IMTG) during exercise compared with men. To investigate whether this could be due to gender-specific regulation of HSL and to use gender-comparison as a model to gain further insight into HSL regulation, nine women and eight men performed bicycle exercise (90 min, 60% VO2 peak), and skeletal muscle HSL expression, phosphorylation, and activity were determined. Supporting previous findings, basal IMTG content (p
