Electrophisiology curves or line-scan images can be analyzed automatically or
manually overproofed data can be generated. • Smoothing and coloring can be ...
Femto2D MULTIPHOTON LASER SCANNING MICROSCOPE
Glutamate receptor mapping using Femto2D microscopes
© Femtonics Kft. Tűzoltó u. 59. H-1094 Budapest Hungary
www.femtonics.eu
[email protected] +36-1-2103349
Glutamate receptor mapping by Femto2D microscopes
• Select sites in rasters for automatic two-photon glutamate uncaging. Each triangle shows different uncaging locations used in the measurement series • Do a series of uncaging measurements using the LineMagic module. • At each uncaging location a little zigzag patterned photostimulation is delivered according to the preset protocol in order to release glutamate in 300 µs
Voltage Clamp (pA)
2µm
0
• Raw somatic current traces measured following glutamate uncaging in locations shown above
-20
-40 -10 -5
0
5 10 T[ms]
15
20
• Quickly analyze data using the UncageMapping module. • Electrophisiology curves or line-scan images can be analyzed automatically or manually overproofed data can be generated • Smoothing and coloring can be changed
copyright 2011 © Femtonics Kft.
[email protected] | Tűzoltó u. 59. H-1094 Budapest, Hungary | +36-1-2103349 | www.femtonics.eu
Glutamate sensitivity map of a pyramidall cell Mapping of glutamate receptors in CA1 pyramidal cell by simultaneous two-photon glutamate uncaging and somatic current recording using a Femto2D microscope Raw EPSC map
Smoothed EPSC map, with siluet of the dendrite
Two-photon fluorescence during uncaging
10µm
Images Courtesy of Gergely Katona, Institute of Experimental Medicine, Hungarian Academy of Sciences
copyright 2011 © Femtonics Kft.
[email protected] | Tűzoltó u. 59. H-1094 Budapest, Hungary | +36-1-2103349 | www.femtonics.eu
Glutamate sensitivity map of an interneuron Mapping of glutamate receptors in CA1 stratum radiatum interneurons by simultaneous two-photon glutamate uncaging and somatic current recording using a Femto2D microscope
Raw EPSC map -25
-20
-15
Green during uncaging
2μm
EPSC (pA)
Smoothed EPSC map
-10
Range: 4-36pA -5
Images Courtesy of Gergely Katona, Institute of Experimental Medicine, Hungarian Academy of Sciences See published: G. Katona, A. Kaszása, G. F. Turi, N. Hájos, G. Tamás, E.S. Vizi, B. Rózsa Roller Coaster Scanning reveals spontaneous triggering of dendritic spikes in CA1 interneurons, Proc. Natl. Acad. Sci. USA,Volume 108, No. 5 (2011), Page 2148-2153 http://dx.doi.org/10.1073/pnas.1009270108 copyright 2011 © Femtonics Kft.
[email protected] | Tűzoltó u. 59. H-1094 Budapest, Hungary | +36-1-2103349 | www.femtonics.eu
