Articles in PresS. J Appl Physiol (November 21, 2003). 10.1152/japplphysiol.01003.2003
GREATER EFFECT OF DIET THAN EXERCISE TRAINING ON THE FATTY ACID PROFILE OF RAT SKELETAL MUSCLE
Nigel Turner1,2, Jong Sam Lee 4, Clinton R. Bruce4, Todd W. Mitchell1,2, Paul L. Else1,2, A.J. Hulbert1,3 and John A. Hawley4.
1
Metabolic Research Centre, and Departments of Biomedical2 & Biological3 Sciences,
University of Wollongong, Wollongong, N.S.W. 2522, Australia; 4Exercise Metabolism Group, School of Medical Sciences, RMIT University, Bundoora 3083, Australia.
Running head: Diet, exercise and fatty acid profile
Address for correspondence: John A. Hawley, Ph.D. Exercise Metabolism Group School of Medical Sciences RMIT University PO Box 71 Bundoora 3083 Australia. Email:
[email protected] Fax:
+61-3-9467 8181
Phone: +61-3-9925 7353
Copyright (c) 2003 by the American Physiological Society.
2 ABSTRACT We determined the interaction of diet and exercise-training intensity on membrane phospholipid fatty acid (FA) composition in skeletal muscle from 36 female Sprague-Dawley rats. Animals were randomly divided into one of two dietary conditions; high-carbohydrate (CHO; 64.0% carbohydrate E, n=18) or high-fat (FAT; 78.1% fat E, n=18). Rats in each diet condition were then allocated to one of three subgroups: control, that performed no exercisetraining (NT); low-intensity (8 m.min-1) treadmill run training (LOW); or high-intensity (28 m.min-1) run training (VMAX). All exercise-trained rats ran 1,000m. session-1, 4 d.wk-1 for 8 wk and were sacrificed 48 h after the last training bout. Membrane phospholipids were extracted and FA composition was determined in the red (RVL) and white vastus lateralis (WVL) muscles. Diet exerted a major influence on phospholipid FA composition, with the FAT diet being associated with a significantly (P
