1Department of Microbiology, Ibrahim Badamasi Babangida University, Lapai. 2Science Laboratory Technology Department, Federal polytechnic Bida.
Heavy Metal Tolerance of Bacteria Isolated From Mechanic Workshops Mohammed, J. N1*, Aliyu M. B2, Kasim Z. J1 and Gana, M1. Department of Microbiology, Ibrahim Badamasi Babangida University, Lapai 2 Science Laboratory Technology Department, Federal polytechnic Bida Corresponding author: +2347067855089
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Abstract: A study was conducted to determine the heavy metal tolerance of bacteria isolated from soil samples of mechanic workshops. Soil samples from mechanic workshops were serially diluted and cultured on nutrient agar. The isolates were characterized and identified using conventional biochemical tests. The isolates were pour plated on nutrient agar and four holes were bored in the media using a sterile cork borer. Four concentrations (1.00mg/ml, 0.75mg/ml, 0.50mg/ml and 0.25mg/ml) were introduced into the holes and incubated for 24hours. The isolated bacteria were Pseudomonas sp, Bacillus sp and Micrococcus sp. Pseudomonas sp had better tolerance to Chromium Chloride. At concentrations 0.50mg/ml and 0.25mg/ml of Chromium chloride. there was no zone of inhibition by the metal on Pseudomonas and Bacillus sp when compared with Micrococcus sp that has 15mm for both concentrations. The result from this study also showed that Micrococcus sp has higher tolerance to Copper II Chloride than other isolated bacteria. The overall microbial tolerance of these bacteria indicates that Pseudomonas sp could tolerate ferrous sulphate and chromium chloride than Micrococcus sp. Bacterial tolerance were significantly different (p
