Jun 16, 2005 - The human kallikrein 4 gene (KLK4)5 is a member of the human kallikrein family. The expression of this gene,. 1 Department of Pathology and ...
Papers in Press. First published June 16, 2005 as doi:10.1373/clinchem.2005.049692
Clinical Chemistry 51:8 000 – 000 (2005)
Proteomics and Protein Markers
Human Kallikrein 4: Quantitative Study in Tissues and Evidence for Its Secretion into Biological Fluids Christina V. Obiezu,1,2 Shannon J.C. Shan,1,2 Antoninus Soosaipillai,1 Liu-Ying Luo,1,2 Linda Grass,1 Georgia Sotiropoulou,3 Constantina D. Petraki,4 Panos A. Papanastasiou,4 Michael A. Levesque,1 and Eleftherios P. Diamandis1,2*
Background: Human kallikrein 4 (hK4) is a proteolytic enzyme belonging to the tissue kallikrein family of serine proteases. Previous tissue expression studies have demonstrated highest KLK4 mRNA expression in prostatic tissue, but there has been only limited evidence for the presence of hK4 protein in prostate and other tissues and in corresponding biological secretions. Methods: To investigate the concentrations of hK4 in tissues and biological fluids, we developed a new hK4specific sandwich-type immunoassay using a monoclonal antibody as the capture reagent. Results: The assay has a detection limit of 0.02 g/L and
