PGM1, GLO, HLA, and PGM3. There is some evidence for possible linkage with MNSs, Rh, Gm and EsD. Family segregation data confirm the hypothesis for-.
Hum. Genet. 53, 421-423 (1980) © by Springer-Verlag1980
Human Phosphoglycolate Phosphatase (PGP) E.C. 3.1.3.18: Linkage Analysis G. Siebert*, A. Amorim**, and J. K 6 m p f Institut ffir Anthropologie und Humangenetik der Universitfit Tiibingen, Wilhelmstrasse 27, D-7400 Ttibingen, Federal Republic of Germany
Summary. Linkage data on phosphoglycolate phosphatase (PGP) E.C. 3.1.3.18 and 26 other h u m a n genetic markers are presented. One hundred and one families from the southwestern area of Germany were tested. Close linkage between P G P and the following markers could be ruled out: AB0, acP, ADA, GPT, PGM1, G L O , H L A , and PGM3. There is some evidence for possible linkage with MNSs, Rh, G m and EsD. Family segregation data confirm the hypothesis formerly established by Barker and Hopkinson: three common alleles P G P ~, P G P 2 and P G P 3 at an autosomal locus PGP.
Materials and Methods One hundred and one families from the southwestern area of Germany were tested. Sample preparation electrophoresis and staining procedure was performed as described by Amorim et al. (in press).
Results and Discussion Table 1 shows the segregation of P G P in 101 families. With respect to the mating type 1 x 2-1 the segregation of the children's phenotypes seems to be symmetrical. Though more data on the rare mating types are required these family results do agree with the hypothesis that there are three alleles P G P ~, P G P 2, P G P 3 at an autosomal locus PGP, and thus support the results given by Barker and Hopkinson (1978) and Amorim et al. (in press).
Introduction Phosphoglycolate phosphatase (PGP) was first described in plant tissues (Richardson and Tolbert, 1961; Randall et al., 1971). Later Badwey (1977) was able to demonstrate enzyme activity in human erythrocytes. Recently Barker and Hopkinson (1978) reported a genetically determined polymorphism of P G P in a population sample of European origin, and stated that family studies indicate the presence of three alleles P G P 1, P G P 2, P G P 3 at an autosomal locus PGP. We here present data on linkage analysis as well as a family segregation analysis. Offprint requests to: G. Siebert
* Supported by the Deutsche Forschungsgemeinschaft ** Supported by DAAD and Portuguese Inst. for Scientific Research (INIC)
Linkage Analysis Lod scores were calculated according to MaynardSmith tables (1961). Table 2 shows the linkage relations between P G P and 26 other human genetic markers. Z-scores are given for both sexes combined assuming Hardy-Weinberg conditions. Close linkage (0
