Roberto Bellelli, Valerie Borel, Clare Logan, Jennifer Svendsen, Danielle E. Cox, ... Nye, Kay Metcalfe, Susan M. O'Connell, Gordon Stamp, Helen R. Flynn, ...
Molecular Cell, Volume 70
Supplemental Information
Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis Roberto Bellelli, Valerie Borel, Clare Logan, Jennifer Svendsen, Danielle E. Cox, Emma Nye, Kay Metcalfe, Susan M. O'Connell, Gordon Stamp, Helen R. Flynn, Ambrosius P. Snijders, François Lassailly, Andrew Jackson, and Simon J. Boulton
Figure S1 A.
B.
Pole4 (inbred) +/-
+/+
Pole4 (inbred) -/-
(127)
36.0
(25)
63.6
(50)
-/-
11.5dpc
+/+
161
2.0
4.4 4.2 4.0 3.8 Pole4+/+ Pole4-/-
0.35 0.30
0.70
0.60
0.60
0.50 0.45
Pole4+/+ Pole4-/-
0.55
Pole4+/+ Pole4-/-
0.55
0.60 0.55 0.50
Pole4+/+ Pole4-/-
Pole4+/+ Pole4-/-
G.
F. 90.0
Pole4+/+ Pole4+/Pole4-/-
80.0
40.0 30.0
n=16
Pole4+/+ Pole4-/-
-/n=16
10000
I.
5000
20.0
0.40
p=0.01 15000
50.0
0.45
H. +/+
70.0 60.0
0.50
20000
Time on rotarod (ms)
Mice (%)
0.25
0.65
0.65
0.55
Pole4+/+ Pole4-/-
0.30
Pole4+/+ Pole4-/-
Humerus/femur length
0.40
0.35
Pole4-/-
0.6
Pole4+/+ Pole4-/-
Pelvis/femur length
Tibia/femur length
0.45
100
0.40
0.7
1.4
0.65
0.50
(25)
0.8
1.6
1.2
0.4
Pole4+/+
Radius/femur length
3.6
253
p5 gH2AX foci (** p< 0.01; *** p< 0.001); Error bars ±standard deviation (SD) are included; Scale bar, 30µM. (C) Left: representative 53BP1 immunofluorescence staining of CTR1 and POLE1 mutant (P1 and P2) cells. DAPI was used for nuclear counterstaining. Right: Bar graph showing percentage of CTR and POLE1 mutant cells with >3 53BP1 foci (** p< 0.01; *** p< 0.001); Error bars ±standard deviation (SD) are included; Scale bar, 30µM.
