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Culture Media in ISO 6579 Buffered Peptone Water Cat. 1402 RVS Broth Cat. 1174
MKTTn Broth Cat. 1173
XLD Agar Cat.1274 & Salmonella Chromogenic Agar Cat.1122
BUFFERED PEPTONE WATER Peptones / Salts / Buffer Incubate at 37±1°C during 18 ± 2 h
Nutrient Agar Cat. 1060 Biochemical Test
Medium rich in nutrients
Allows the detection of small number of Salmonella or injured
Salmonella
Culture Media in ISO 6579 Buffered Peptone Water Cat. 1402 RVS Broth Cat. 1174
MKTTn Broth Cat. 1173
XLD Agar Cat.1274 & Salmonella Chromogenic Agar Cat.1122
Nutrient AgarCat. 1060 Biochemical Test
RVS Broth (Rappaport Soja acc VASSILIADIS) Peptone / Salts / Buffer Magnesium chloride / Malaquite green 0.1 ml of the incubated buffered peptone water is transfered into 10 ml of RVS Broth. Incubate at 41.5±1°C during 24±2 hours
Malaquite green inhibits the growth of accompanying flora
Magnesium cloride allows a better growth of
Salmonella
After incubation there is a lot of Salmonella cells
Culture Media in ISO 6579 Buffered Peptone Water Cat. 1402 RVS Broth Cat. 1174
MKTTn Broth Cat. 1173
XLD Agar Cat.1274 & Salmonella Chromogenic Agar Cat.1122
Nutrient Agar Cat. 1060 Biochemical Test
MKTTn Broth Peptone / Salts / Buffer Calcium carbonate / Tetrationate / Ox bile / Brilliant Green / Novobiocine 1 ml of the incubated buffered peptone water is transfered into 10 ml tube of MKTTn Broth. Incubate at 37±1°C during 24±2 hours Tetrationate inhibits the growth of intestinal bacteria
Bile stimulate Salmonella growing and inhibits accompanying flora
Brilliant green prevents the growth of Gram negative bacteria
Novobiocine inhibits the
accompanying flora
After incubation there are a lot of Salmonella cells
Culture Media in ISO 6579 Buffered Peptone Water Cat. 1402 RVS Broth Cat.
1174
MKTTn Broth Cat. 1173
XLD Agar Cat.1274 & Salmonella Chromogenic Cat.1122
XLD Agar (Xilose Lisine Desoxicholate) Peptones / Salts / Buffer Xilose, Lactose, Sucrose & Phenol Red / Sodium Thiosulfate y Ferric Ammonium Citrate / L-lysine Incubate at 37±1°C during 24±3 hours
Nutrient Agar Cat. 1060 Biochemical Test
If Xilose, lactose and sucrose are fermented, the pH decrease, changing the color of phenol red, that is a pH indicator
Sodium Thiosulfate provides Sulphur and Ferric ammonium citrate is the indicator for H22S production
Lisine is decarboxilated, producing cadaverine, which increases the pH and forming a purple halo around the colony
Salmonella growth in black centered red colonies
Culture Media in ISO 6579 Buffered Peptone water Cat. 1402 RVS Broth
Cat. 1174
MKTTn Broth Cat. 1173
XLD Agar Cat.1274 & Salmonella Chromogenic Agar Cat.1122
SALMONELLA CHROMOGENIC AGAR Peptones / Salts / Chromogenic Substrate Incubate at 35±1°C during 1824 hours
Nutrient Agar Cat. 1060 Biochemical Test
Esterase activity cleaves one of the chromogenic substrates, giving the colony a magenta color
β-Galactosidase activity cleaves the other chromogenic substrate, giving the colony a green color
Salmonella growth in magenta colonies
Culture Media in ISO 6579 Buffered Peptone water Cat. 1402 RVS Broth Cat. 1174
MKTTn Broth Cat. 1173
XLD Agar Cat.1274 & Salmonella Chromogenic Agar Cat.1122
NUTRIENT AGAR Peptone / Salts / Buffer Incubate at 35±1°C during 2448 hours
Nutrient Agar Cat. 1060 Biochemical Test
Medium rich in nutrients
Isolated colonies for biochemical tests
Culture Media in ISO 6579 Buffered Peptone water Cat. 1402 RVS Broth Cat. 1174
MKTTn Broth Cat. 1173
XLD Agar Cat.1274 & Salmonella Chromogenic Agar Cat.1122
TSI AGAR Salt / Buffer Glucose / Lactose & Sucrose Phenol Red / Ferric ammonium citrate & Sodium thiosulfate Incubate at 35±2°C during 1824 hours
Nutrient Agar Cat. 1060 Biochemical Test
Glucose fermentation turns the medium into yellow and may product gas
Lactose and Sucrose fermentation turns the medium into red
Sodium Thiosulfate provides Sulphur and Ferric Ammonium citrate, which is the indicator for H2S production
Culture Media in ISO 6579 Buffered Peptone water Cat. 1402 RVS Broth Cat. 1174
MKTTn Broth Cat. 1173
XLD Agar Cat.1274 & Salmonella Chromogenic Agar Cat.1122
LYSINE DECARBOXYLASE BROTH Glucose / Bromocresol Purple / Lisine Incubate at 37±1°C during 24 ± 3 hours
Nutrient Agar Cat. 1060 Biochemical Test
Glucose fermentation turns the medium into yellow
Lisine is decarboxilated, producing cadaverine, which increases the pH and forms a purple halo around the colony
Tubes with a positive reaction to lisine decarboxilation will present a purple color
