C51/172S/H-Ras. Prdx1+/+. Prdx1-/-. 25. 20. 15. 10. 5. 0. H. 2. O. 2. (n m o l)/1. 0. ^. 6 c e lls. 50. 150. 100. 200. 0. 250. Time (min). Prdx1-/- Prdx1C51/172S.
A H2O2(nmol)/10^6 cells
25
** *
Prdx1-/- Prdx1C51/172S Prdx1-/-
20
** *
15
Prdx1-/-WT
** *
*
10
5
*
0 0
50
B
100 150 Time (min)
200
250
H2O2 release of Prdx1-/-MEFs (filled square), Prdx1-/-MEFs reconstituted with the Prdx1 wildtype gene (Prdx1WT) (filled triangle) and Prdx1-/-MEFs expressing Prdx1C51/172S (filled circle) were measured as described in detail in Materials and methods, by using Amplex Red reagent, which via oxidation, becomes fluorescent resorufin. The y-Axis presents H2O2 release accumulated over time per 1x10^6 cells. For each clone, 6 wells were plated and analyzed. The experiment shown here is representative of 5 independent studies from 3 different sets of MEF clones from Prdx1-littermates. P values were calculated using an unpaired Student's t-Test. *P
