Our new low-pressure, high performance Poroshell 120 columns can improve the
speed and resolution of any system—including yours. And new Agilent 1200 ...
It’s the latest revolution in
liquid chromatography. And you’re right at the center of it.
Our measure is your success.
products | applications | software | services
The new LC solutions from Agilent all revolve around your needs. It’s all about you—and getting the performance, productivity and value you’re looking for from your liquid chromatography investment. Whatever industry you’re in, whatever your application—we think you should be able to get to the next level of LC from wherever you happen to be. That the path to better data quality and faster, more confident results should start with your current system, not with a total hardware upgrade or wholesale change of methods. That’s the whole idea behind the newest additions to Agilent’s world-class LC and LC/MS portfolio. Our new low-pressure, high performance Poroshell 120 columns can improve the speed and resolution of any system—including yours. And new Agilent 1200 Series enhancements deliver even more capabilities for both conventional LC and Rapid Resolution LC, while letting you keep all your options open. Wherever you are today, we can help you build on what you have and make it work even harder. No other company gives you a wider range of LC and LC/MS choices. And no other platform lets you so fully optimize your application, while perfectly aligning analysis capabilities with your workflows and your budget. The revolution starts now. With you.
Efficiency
Performance
Turn the page and see how Agilent’s new LC solutions give you more. • More separation power for both RRLC and conventional LC with Agilent’s new, lower pressure Poroshell 120 columns
• More robust nanoflow separations with Agilent’s innovative HPLC-Chip technology and new smallmolecule applications
• More methods development control and flexibility with the new 1200 Series automated method development system
• More powerful LC/MS and LC/MS/MS capabilities with our full line of 6000 Series mass spectrometers
• More detection capabilities—including new UV/Vis and ELSD additions to the 1200 Series lineup • More autosampler performance and near-zero carry-over with the new 1200 Series High Performance Autosampler SL Plus
• More control and data management functionality with the latest enhancements to ChemStation software • More uptime and optimized column and consumables usage with Lab Monitor & Diagnostic software
Budget
Selectivity
Time
Resolution
Get more perfo Maximum separation power—90,000 plates in 4 minutes! The low backpressure of the new Poroshell 120 columns lets you couple several columns together in series, achieving highest separation power per time (90,000 plates in 4 minutes). This enables better separation of complex samples, and makes it possible to resolve more compounds than ever before. Higher speed and efficiency for all types of systems
New Agilent Poroshell 120 Columns: higher productivity, lower pressure Agilent’s breakthrough new Poroshell 120 columns, an extension of the Poroshell 300 portfolio, can enhance the productivity of almost any LC application. Operating at 40 –50% less pressure than conventional high resolution columns, they give you the separation efficiency of a sub-2 µm particle—without the need to upgrade to a high-pressure system!
Delivering the efficiency of a 1.8 µm column at lower pressure, Poroshell 120 columns can accommodate different instrument configurations, applications and sample types. Use them with a standard LC system to achieve higher speeds and better resolution. Use them with a high pressure, ultra-fast system to achieve maximum efficiency and resolution—and even faster separations. Better robustness for all types of samples Standard frits and larger pore sizes reduce the risk of column clogging, even with clinical samples or other dirty matrices. We offer multiple choices for outstanding peak shape—an endcapped highly deactivated column and a non-endcapped version in the popular C18 phase.
Agilent ZORBAX 1.8 µm Rapid Resolution HT columns are still the best choice for many ultra-fast, high-throughput applications. For maximum methods flexibility, Agilent offers more than 140 RRHT, 1.8 µm column choices: An inside look at Poroshell 120. The 2.7 µm particle has a solid core (1.7 µm) and porous 0.5 µm outer layer, providing a shorter diffusion path and more efficient mass transfer.
• 14 different column chemistries—13 bonded phases and silica for HILIC • 6 column lengths (150, 100, 75, 50, 30 and 20 mm) • 3 internal diameters (4.6, 3.0 and 2.1 mm ID) • Matching 3.5 µm, 5 µm and 7 µm choices for simple, predictable scale-up and bidirectional methods transfer between conventional HPLC and RRLC
ormance from your current LC system. New detection options: Higher sensitivity, faster detection New Agilent 1200 Series Variable Wavelength Detector SL Plus
3x increase in sensitivity. Redesigned optical components provide highest signal levels and lowest baseline noise.
Agilent 1200 Series LC Method Development Solution The new Agilent 1200 Series method development solution saves hours of lab time by enabling you to determine optimum separation methods without tedious manual column and solvent changing. The highly flexible system—entirely composed of standard Agilent 1200 Series modules—offers customized workflow solutions ideally suited to any LC method development group, and allows you to: • Fully automate your methods development, from modeling through experimental proof—design complex method-scouting campaigns with a few mouse clicks, and screen methods overnight • Automatically select up to eight columns and up to 15 different solvents • Quickly optimize temperature selection with up to 6 independent column temperature zones and thermostatted valves • Extend your method development capabilities with optional 6100 Series quadrupole LC/MS Using two thermostatted column compartments and selection valves, you can combine up to eight columns of up to 100 mm in length, or six columns with a waste port and bypass. Adding a third compartment allows using columns longer than 100 mm.
With sampling rates up to 160 Hz, the new Agilent 1200 Series variable wavelength detector SL Plus can keep up with the fastest LC analysis. Best-in-class noise and drift specifications give you maximum sensitivity for lowest detection limits and precise quantification at trace levels. An extremely wide linear range ensures reliable, simultaneous detection and measurement of primary compounds, by-products and impurities. New Agilent 1200 Series Evaporative Light Scattering Detector
Detection of semi-volatile parabens separated by RRLC.
Fully integrated with Agilent ChemStation software, the Agilent 1200 Series ELSD enables highly sensitive detection of non-UV-absorbing compunds. The detector essentially provides a universal measurement under isocratic and gradient conditions—independent of a compound’s absorbance, fluorescence or electroactivity. Minimized peak broadening ensures compatibility with ultra-fast RRLC.
And your next system, too. New 1200 Series High Performance Autosampler SL Plus: Near-zero sample carryover
After an injection of 240 ng of chlorhexidine, carryover was found to be 0.0028%, equivalent to 8 pg.
The Agilent 1200 Series high performance autosampler SL Plus has been extensively re-engineered to maximize performance and productivity in demanding RRLC and high-sensitivity LC/MS applications. Based on a proven flow-through design that ensures a wide injection volume range and the possibility to inject small volumes without loss of sample, the new SL Plus model achieves near-zero carryover—typically less than 0.004 %.
Agilent HPLC-Chip makes nanoflow LC/MS as easy as “plug and play.” Working seamlessly with the 1200 Series LC and 6000 Series LC/MS systems, Agilent’s revolutionary HPLCChip integrates the sample enrichment and separation columns of a nanoflow LC system with the intricate connections and nanospray tip used in electrospray mass spectrometry—all on a single, reusable microfluidic chip about the size of a microscope slide. Reusable chips are available in multiple functionalities (including custom configurations) to enable easy, interchangeable workflows with minimum downtime and maximum productivity. New small molecule applications complement the popular proteomics selections. Versatile, easy to use HPLC-Chip platform offers unprecedented plug-and-play flexibility. So you can quickly and easily switch between different projects—or different researchers—in minutes.
Agilent’s LC/MS portfolio includes Single Quadrupole, Ion Trap, Triple Quadrupole, TOF and Q-TOF solutions.
Agilent 6000 Series LC/MS systems: A Mass Spec/tacular lineup! If you’re looking for more sensitive target compound analysis and better identification of unknowns, take a close look at Agilent’s comprehensive LC/MS portfolio. Our 6000 Series mass spectrometers are perfectly matched with our 1200 Series LC to deliver exceptional results—routinely. • To match your lab’s analysis needs, choose from a full line of best-in-class LC/MS systems: single quadrupole, ion trap, triple quadrupole, TOF and Q-TOF • Select from the industry’s widest range of ionization sources—all of them easily interchangable—so you can ionize and measure almost all compound classes • Agilent MassHunter software gives you intuitive instrument control, compound-centric data processing, as well as workflowdriven qualitative and quantitative data analysis and reporting
A complete portfolio of software solutions Agilent’s comprehensive software and services solutions for laboratories and enterprises reduce the time, effort and cost to go from raw data to final insight.
Enterprise Application Integration Analytical
New ChemStation software: A perfect fit for your workflow and your application Agilent ChemStation software makes it easy even for non-expert operators to take full advantage of the powerful capabilities of any Agilent 1200 Series LC system. From the friendly, familiar interface to new, advanced Level-5 instrument control, you’ll find everything designed to help you make the most of every run and every workday. • Seamless fit in both regulated and non-regulated environments (including support for 21 CFR part 11 compliance) • Easily extendable functionality through built-in macro language • Enhanced workflow support and user-customizable capabilities • Outstanding usability and faster results review • Integration of third-party detectors (ESA, CAD, CCIII) • Control of Agilent LC, GC, CE, CE/MS and LC/MS instruments, as well as generic A/D converters
Biology
Chemistry
Laboratory Workflows Chromatography, Instrumentation, Spectrometry Enterprise Content Management Service & Support Agilent’s Software Solutions integrate all your lab’s applications, instruments and data.
• Agilent OpenLAB operating system for the lab, including instrument control, advanced reporting, enterprise content management, business process management • Agilent EZChrom Elite CDS for labs with instruments from multiple vendors • Agilent Kalabie Electronic Lab Notebook (ELN) for simplified and streamlined knowledge creation and data management
• Integration with Agilent OpenLAB Enterprise Content Manager (ECM) for secure, centralized storage of all electronic data generated in your organization • Advanced reporting of ChemStation data through Agilent OpenLAB Intelligence Reporter—including complex calculations, matrix-style tables, as well as grouping, sorting and filtering of result data
Agilent’s flexible, modular architecture lets you add exactly the functionality your lab needs, as you need it.
The Agilent 1200 Series. One flexible platform for all your LC needs.
To learn more about the newest Agilent LC solutions for your lab, visit www.agilent.com/chem/futurelcnow.
Agilent: One step ahead in LC technology, right in step with you Whatever your liquid chromatography needs, our integrated portfolio of instruments, applications, software and industryleading services can help your lab generate better results, faster than ever. From our new low-pressure, high performance Poroshell columns, to our enhanced 1200 Series modules for conventional LC, and all the latest innovations for Rapid Resolution LC—Agilent can help you get more performance from your current system. And your next system, too.
Lab Monitor & Diagnostic software: eliminate problems before they happen Innovative Lab Monitor & Diagnostic software continuously monitors all of your lab’s Agilent LC and LC/MS systems in real time to alert you to maintenance needs and instrument problems—before they can affect your results. The software also keeps track of injections, hours of operation and other user-specified parameters. It helps you optimize column and consumables replacement schedules, and lets you know when it’s time to perform basic upkeep tasks. It makes it quick and easy to perform automated diagnostic routine, and helps you meet regulatory requirements by keeping all maintenance, event and run logs in a single, easily accessible location.
The Agilent Service Advantage: Helping you focus on what you do best In addition to industry-leading products, workflow-driven applications and powerful, intuitive software, Agilent brings you a worldwide network of highly skilled, experienced professionals committed to your success. Whether you need support for a single instrument or a multi-laboratory operation, we can help you solve problems quickly, increase your uptime and optimize your lab’s resources.
For more information Learn more: www.agilent.com/chem/futurelcnow Buy online: www.agilent.com/chem/store Find an Agilent customer center in your country:
www.agilent.com/chem/contactus U.S. and Canada 1-800-227-9770
[email protected] Europe
[email protected] Asia Pacific
[email protected] © Agilent Technologies, Inc. 2008 Printed in USA July 1, 2008 5989-8876EN
